Cas9 & gRNA Delivery

Find the best Cas9 and gRNA delivery options for your genome engineering application—choose from mRNA, lentivectors, protein, AAV, and more

With a wealth of options for delivering Cas9 and gRNA, SBI supports a full range of genome engineering applications. Deliver Cas9 and gRNA via mRNA, lentivector, or AAV, or use purified Cas9 protein, and then choose from our all-in-one systems that deliver Cas9 and gRNA on a single vector or two-component systems for separate introduction of Cas9 and gRNA.

Not sure which system to choose? Take a look at our table below, or contact technical support—we’re here to help you find the right product for your application and can provide expert experimental support.

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Cas9 & gRNA Delivery Products

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CAG-hspCas9-H1-gRNA All-in-one Cas9 SmartNuclease Plasmid (circular)

SBI’s first generation Cas9 vector places hspCas9 under the control of the CAG promoter and is now available as a circular plasmid
Size:10 µg (Cat. # CAS920A-1)

CMV-hspCas9-H1-gRNA All-in-one Cas9 SmartNuclease Plasmid (circular)

SBI’s first generation Cas9 vector places hspCas9 under the control of the CMV promoter and is now available as a circular plasmid
Size:10 µg (Cat. # CAS940A-1)

MSCV-hspCas9-H1-gRNA All-in-one Cas9 SmartNuclease Plasmid (circular)

SBI’s first generation Cas9 vector places hspCas9 under the control of the MSCV promoter and is now available as a circular plasmid
Size:10 µg (Cat. # CAS960A-1)

EF1α-T7-hspCas9-H1-gRNA All-in-one Cas9 SmartNuclease™ Plasmid

Clone your own gRNA into this vector and simplify delivery of hspCas9 and gRNA.
Size:10 Reactions (Cat. # CAS700A-1)

PGK-hspCas9-H1-gRNA All-in-one Cas9 SmartNuclease Plasmid (circular)

SBI’s first generation Cas9 vector places hspCas9 under the control of the PGK promoter and is now available as a circular plasmid
Size:10 µg (Cat. # CAS980A-1)

EF1α-T7-hspCas9-H1-gRNA All-in-one Cas9 SmartNuclease™ Plasmid & Multiplex gRNA Cloning Kit

Easily create an All-in-one Cas9 SmartNuclease Plasmid that delivers multiple gRNAs—kit bundles EF1α-T7-hspCas9-H1-gRNA with the Multiplex gRNA Cloning Kit
Size:10 Reactions (Cat. # CAS700A-KIT)

EF1α-hsaCas9-U6-gRNA(SA) All-in-one AAV Cas9 SmartNuclease™ Plasmid

Extend genome editing capabilities to cutting edge in vivo applications with our All-in-one AAV Cas9 SmartNuclease Plasmid EF1α-hsaCas9-U6-gRNA(SA).
Size:10 Reactions (Cat. # CASAAV100PA-1)

EF1α-T7-hspCas9-T2A-GFP-H1-gRNA All-in-one Cas9 SmartNuclease™ Plasmid

Easily verify transfection efficiencies with this All-in-one Cas9 SmartNuclease Plasmid that coordinately expresses GFP - EF1α-T7-hspCas9-T2A-GFP-H1-gRNA
Size:10 Reactions (Cat. # CAS700G-1)

EF1α-hsaCas9 Two Vector AAV Cas9 SmartNuclease™ Expression Plasmid

Extend genome editing capabilities to cutting edge in vivo applications with our Two Vector AAV Cas9 SmartNuclease Expression Plasmid EF1α-hsaCas9.
Size:10 µg (Cat. # CASAAV200PA-1)

EF1α-T7-hspCas9-T2A-RFP-H1-gRNA All-in-one Cas9 SmartNuclease™ Plasmid

Easily verify transfection efficiencies with this All-in-one Cas9 SmartNuclease Plasmid that coordinately expresses RFP- EF1α-T7-hspCas9-T2A-RFP-H1-gRNA
Size:10 Reactions (Cat. # CAS701R-1)

CMV-hspCas9-T2A-Puro Two Vector Cas9 SmartNuclease™ Lentivector

When working with difficult-to-transfect-cells, use our lentiviral Cas9 SmartNuclease System. This vector drives Cas9 expression with CMV and has Puro selection.
Sizes:10 µg (Cat. # CASLV100PA-1), 1 Kit (Cat. # CASLV100PA-KIT), 2 x 25 µL (Cat. # CASLV100VA-1)

CAG-T7-hspCas9-H1-gRNA All-in-one Cas9 SmartNuclease™ Plasmid

Clone your own gRNA into this vector and simplify delivery of hspCas9 and gRNA.
Size:10 Reactions (Cat. # CAS720A-1)

CMV-hspCas9-EF1α-GFP Two Vector Cas9 SmartNuclease™ Lentivector

When working with difficult-to-transfect-cells, use our lentiviral Cas9 SmartNuclease System. This vector drives Cas9 expression with CMV and has a GFP marker.
Sizes:10 µg (Cat. # CASLV105PA-1), 1 Kit (Cat. # CASLV105PA-KIT), 2 x 25 µL (Cat. # CASLV105VA-1)

CAG-T7-hspCas9-H1-gRNA All-in-one Cas9 SmartNuclease™ Plasmid & Multiplex gRNA Cloning Kit

Easily create an All-in-one Cas9 SmartNuclease Plasmid that delivers multiple gRNAs – kit bundles CAG-T7-hspCas9-H1-gRNA with the Multiplex gRNA Cloning Kit
Size:10 Reactions (Cat. # CAS720A-KIT)

MSCV-hspCas9-T2A-Puro Two Vector Cas9 SmartNuclease™ Lentivector

When working with difficult-to-transfect-cells, use our lentiviral Cas9 SmartNuclease System. This vector drives Cas9 expression with MSCV and has Puro selection.
Sizes:10 µg (Cat. # CASLV120PA-1), 1 Kit (Cat. # CASLV120PA-KIT), 2 x 25 µL (Cat. # CASLV120VA-1)

CAG-T7-hspCas9-T2A-GFP-H1-gRNA All-in-one Cas9 SmartNuclease™ Plasmid

Easily verify transfection efficiencies with this All-in-one Cas9 SmartNuclease Plasmid that coordinately expresses GFP—CAG-T7-hspCas9-T2A-GFP-H1-gRNA
Size:10 Reactions (Cat. # CAS720G-1)

MSCV-hspCas9-EF1α-GFP Two Vector Cas9 SmartNuclease™ Lentivector

When working with difficult-to-transfect-cells, use our lentiviral Cas9 SmartNuclease System. This vector drives Cas9 expression with MSCV and has a GFP marker.
Sizes:10 µg (Cat. # CASLV125PA-1), 1 Kit (Cat. # CASLV125PA-KIT), 2 x 25 µL (Cat. # CASLV125VA-1)

CAG-T7-hspCas9-T2A-RFP-H1-gRNA All-in-one Cas9 SmartNuclease™ Plasmid

Easily verify transfection efficiencies with this All-in-one Cas9 SmartNuclease Plasmid that coordinately expresses RFP-CAG-T7-hspCas9-T2A-RFP-H1-gRNA
Size:10 Reactions (Cat. # CAS721R-1)

CMV-hspCas9-T2A-Puro-H1-gRNA All-in-one Cas9 SmartNuclease™ Lentivector

Get the convenience of all-in-one Cas9 & gRNA delivery in a lentivector and streamline genome editing in hard-to-transfect-cells—CMV-hspCas9-T2A-Puro-H1-gRNA.
Size:10 Reactions (Cat. # CASLV300PA-1)

CMV-T7-hspCas9-H1-gRNA All-in-one Cas9 SmartNuclease™ Plasmid

Clone your own gRNA into this vector and simplify delivery of hspCas9 and gRNA.
Size:10 Reactions (Cat. # CAS740A-1)

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