Cas9 & gRNA Delivery

Find the best Cas9 and gRNA delivery options for your genome engineering application—choose from mRNA, lentivectors, protein, AAV, and more

With a wealth of options for delivering Cas9 and gRNA, SBI supports a full range of genome engineering applications. Deliver Cas9 and gRNA via mRNA, lentivector, or AAV, or use purified Cas9 protein, and then choose from our all-in-one systems that deliver Cas9 and gRNA on a single vector or two-component systems for separate introduction of Cas9 and gRNA.

Not sure which system to choose? Take a look at our table below, or contact technical support—we’re here to help you find the right product for your application and can provide expert experimental support.

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Cas9 & gRNA Delivery Products

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CAG-T7-hspCas9-T2A-RFP-H1-gRNA All-in-one Cas9 SmartNuclease™ Plasmid

Easily verify transfection efficiencies with this All-in-one Cas9 SmartNuclease Plasmid that coordinately expresses RFP-CAG-T7-hspCas9-T2A-RFP-H1-gRNA
Size:10 Reactions (Cat. # CAS721R-1)

CMV-T7-hspCas9-Nickase-T2A-RFP-H1-gRNA All-in-one Cas9 SmartNickase™ Plasmid

Easily verify transfection efficiencies with this All-in-one Cas9 SmartNickase Plasmid that coordinately expresses RFP-CMV-T7-hspCas9-Nickase-T2A-RFP-H1-gRNA
Size:10 Reactions (Cat. # CAS791R-1)

CMV-T7-hspCas9-H1-gRNA All-in-one Cas9 SmartNuclease™ Plasmid

Clone your own gRNA into this vector and simplify delivery of hspCas9 and gRNA.
Size:10 Reactions (Cat. # CAS740A-1)

EF1α-hspCas9-nickase-H1-gRNA All-in-one Cas9 SmartNickase Plasmid (circular)

Use this first-generation SBI EF1α-hspCas9 SmartNickase when you want to reduce off-target events, have transfectable cells, and prefer an intact vector.
Size:10 µg (Cat. # CAS800A-1)

CMV-T7-hspCas9-H1-gRNA All-in-one Cas9 SmartNuclease™ Plasmid & Multiplex gRNA Cloning Kit

Easily create an All-in-one Cas9 SmartNuclease Plasmid that delivers multiple gRNAs—kit bundles CMV-T7-hspCas9-H1-gRNA with the Multiplex gRNA Cloning Kit
Size:10 Reactions (Cat. # CAS740A-KIT)

EF1α-hspCas9-DM-H1-gRNA All-in-one Cas9 Null Nuclease Plasmid (circular)

Use the EF1α-hspCas9-DM-H1-gRNA All-in-one Cas9 Null Nuclease Plasmid as a DNA cleavage/DNA nicking-deficient control for your studies.
Size:10 µg (Cat. # CAS805A-1)

CMV-T7-hspCas9-T2A-GFP-H1-gRNA All-in-one Cas9 SmartNuclease™ Plasmid

Easily verify transfection efficiencies with this All-in-one Cas9 SmartNuclease Plasmid that coordinately expresses GFP- CMV-T7-hspCas9-T2A-GFP-H1-gRNA
Size:10 Reactions (Cat. # CAS740G-1)

CAG-hspCas9-nickase-H1-gRNA All-in-one Cas9 SmartNickase Plasmid (circular)

Use this first-generation SBI CAG-hspCas9 SmartNickase when you want to reduce off-target events, have transfectable cells, and prefer an intact vector.
Size:10 µg (Cat. # CAS820A-1)

CMV-T7-hspCas9-T2A-RFP-H1-gRNA All-in-one Cas9 SmartNuclease™ Plasmid

Easily verify transfection efficiencies with this All-in-one Cas9 SmartNuclease Plasmid that coordinately expresses RFP-CMV-T7-hspCas9-T2A-RFP-H1-gRNA
Size:10 Reactions (Cat. # CAS741R-1)

CMV-hspCas9-nickase-H1-gRNA All-in-one Cas9 SmartNickase Plasmid (circular)

Use this first-generation SBI CMV-hspCas9 SmartNickase when you want to reduce off-target events, have transfectable cells, and prefer an intact vector.
Size:10 µg (Cat. # CAS840A-1)

EF1α-T7-hspCas9-Nickase-H1-gRNA All-in-one Cas9 SmartNickase™ Plasmid

Use the EF1α-T7-hspCas9-Nickase-H1-gRNA All-in-one Cas9 SmartNickase plasmid when you want to reduce off-target events and have transfectable cells.
Size:10 Reactions (Cat. # CAS750A-1)

EF1α-hspCas9-H1-gRNA All-in-one Cas9 SmartNuclease Plasmid (circular)

SBI’s first generation Cas9 vector places hspCas9 under the control of the EF1α promoter and is now available as a circular plasmid
Size:10 µg (Cat. # CAS900A-1)

EF1α-T7-hspCas9-Nickase-H1-gRNA All-in-one Cas9 SmartNickase™ Plasmid & Multiplex gRNA Cloning Kit

Easily create an All-in-one Cas9 SmartNickase Plasmid that delivers multiple gRNAs—kit bundles EF1α-T7-hspCas9-Nickase-H1-gRNA with the Multiplex gRNA Cloning Kit
Size:10 Reactions (Cat. # CAS750A-KIT)

CAG-hspCas9-H1-gRNA All-in-one Cas9 SmartNuclease Plasmid (circular)

SBI’s first generation Cas9 vector places hspCas9 under the control of the CAG promoter and is now available as a circular plasmid
Size:10 µg (Cat. # CAS920A-1)

EF1α-T7-hspCas9-Nickase-T2A-GFP-H1-gRNA All-in-one Cas9 SmartNickase™ Plasmid

Easily verify transfection efficiencies with this All-in-one Cas9 SmartNickase Plasmid that coordinately expresses GFP-EF1α-T7-hspCas9-Nickase-T2A-GFP-H1-gRNA
Size:10 Reactions (Cat. # CAS750G-1)

CMV-hspCas9-H1-gRNA All-in-one Cas9 SmartNuclease Plasmid (circular)

SBI’s first generation Cas9 vector places hspCas9 under the control of the CMV promoter and is now available as a circular plasmid
Size:10 µg (Cat. # CAS940A-1)

EF1α-T7-hspCas9-Nickase-T2A-RFP-H1-gRNA All-in-one Cas9 SmartNickase™ Plasmid

Easily verify transfection efficiencies with this All-in-one Cas9 SmartNickase Plasmid that coordinately expresses RF-EF1α-T7-hspCas9-Nickase-T2A-RFP-H1-gRNA
Size:10 Reactions (Cat. # CAS751R-1)

MSCV-hspCas9-H1-gRNA All-in-one Cas9 SmartNuclease Plasmid (circular)

SBI’s first generation Cas9 vector places hspCas9 under the control of the MSCV promoter and is now available as a circular plasmid
Size:10 µg (Cat. # CAS960A-1)

EF1α-T7-hspCas9-H1-gRNA All-in-one Cas9 SmartNuclease™ Plasmid

Clone your own gRNA into this vector and simplify delivery of hspCas9 and gRNA.
Size:10 Reactions (Cat. # CAS700A-1)

CAG-T7-hspCas9-Nickase-H1-gRNA All-in-one Cas9 SmartNickase™ Plasmid

Use the CAG-T7-hspCas9-Nickase-H1-gRNA All-in-one Cas9 SmartNickase plasmid when you want to reduce off-target events and have transfectable cells.
Size:10 Reactions (Cat. # CAS770A-1)

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