Exo-FlowMag96 96-well Magnetic Plate

96-well magnetic plate for Exo-Flow IP Kits—high-throughput, plate-based immunopurification of exosomes from serum, plasma, media, and other biofluids

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Exo-FlowMag96 96-well magnetic plate

1 Kit
EXOFLOWMAG-1
$ 582
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Overview

Simplify your bead-based immunopurifications

Validated to work with any of our Exo-Flow32 or Exo-Flow96 IP Kits, the Exo-FlowMag96 Magnetic Plate simplifies plate-based exosome isolation using magnetic beads.

Use the Exo-Flow Multifunctional Magnetic Stand with any of our Exo-Flow Capture Kits:

Cat.# Kit
EXOFLOW100A-1 CD9 Exo-Flow Capture Kit
EXOFLOW150A-1 Tetraspanin Exo-Flow Combo Capture Kit

EXOFLOW200A-1 CD31 Exo-Flow Capture Kit
EXOFLOW210A-1 CD44 Exo-Flow Capture Kit
EXOFLOW300A-1 CD63 Exo-Flow Capture Kit
EXOFLOW400A-1 CD81 Exo-Flow Capture Kit
EXOFLOW500A-1 Rab5b Exo-Flow Capture Kit
EXOFLOW600A-1 HLA-G Exo-Flow Capture Kit
EXOFLOW610A-1 CD14 Exo-Flow Capture Kit
EXOFLOW620A-1 CD68 Exo-Flow Capture Kit
EXOFLOW630A-1 EpCAM Exo-Flow Capture Kit
EXOFLOW660A-1 CD73 Exo-Flow Capture Kit

How It Works

Take a closer look at the Exo-FlowMag96 96-well Magnetic Plate

How the Exo-FlowMag96 96-well magnetic plate works

Once the Exo-Flow32 or Exo-Flow96 clear plastic plate is placed into the Exo-FlowMag96 96-well Magnetic Plate, the magnetic beads stick to the bottom of each well simplifying removal of buffer for washing and elution.

 

Supporting Data

See isolation data using Exo-FLOW IP Kits

For these studies, either human serum or HEK293 exosomes concentrated from cell culture media using ExoQuick-TC were added to the antibody-coupled magnetic beads. After washing, exosomes were eluted and recovery estimated using a standard BCA protein assay.

Western Blot showing selective immunopurification using Exo-Flow96 IP Kits

Figure 1. CD63 and CD9, two exosome markers, are readily detected in samples purified using Exo-Flow Kits. Approximately 1 µg of protein was loaded per well on a 4-20% gradient protein PAGE. The proteins were separated and transferred to nitrocellulose membranes for Western blot analysis. The blots were probed with either with anti-CD63 or anti-CD9 antibodies to detect the exosome protein markers.

NanoSight analysis shows Exo-Flow Kits deliver expected exosome-sized particles and good yieldsNanoSight analysis shows Exo-Flow Kits deliver expected exosome-sized particlesNanoSight analysis shows Exo-Flow Kits deliver expected exosome-sized particlesNanoSight analysis shows Exo-Flow Kits deliver expected exosome-sized particles

Figure 2. NanoSight analysis shows Exo-Flow IP Kits deliver good yields of particles whose sizes are consistent with exosomes.


Citations

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