SeraMir Exosome RNA Purification Kit

Isolate exosomes and then purify exosomal RNAs for biomarker discovery and more (for serum, plasma, or ascites fluid)

Products

Catalog Number Description Size Price Quantity Add to Cart
RA806A-1 SeraMir Exosome RNA Purification kit (5 mL ExoQuick and 20 exoRNA columns) 20 Preps $511
- +

Overview

Overview

Optimized exosomal RNA extraction for biomarker discovery and more—for serum, plasma, and ascites fluid

Looking for biomarkers? Studying exosomal RNAs (exoRNAs)? Turn to SBI’s SeraMir Exosome RNA Purification Kit for optimized isolation of exoRNAs. The kit includes ExoQuick for efficient exosome isolation from serum, plasma, or ascites fluid, and SeraMir columns and reagents for purification of RNA from your isolated exosomes (for exoRNA isolation from other biofluids, try the SeraMir Exosome RNA Purification Kit for Media and Urine).

Reliable, reproducible exoRNA isolation from serum, plasma, or ascites fluid Find exosomal RNA biomarkers using the SeraMir Exosome RNA Amplification Kit

With cargo that reflects the makeup of their parent cells and their easy isolation, researchers are increasingly turning to exosomes as a source of disease-related biomarkers. To simplify and standardize the isolation of RNA from exosomes, SBI has developed the SeraMir family of products.

The SeraMir Exosome RNA Purification Kit includes everything you need for optimized isolation of exosomal RNAs from serum, plasma, or ascites fluid samples—ExoQuick for fast and efficient exosome isolation and a phenol-free lysis buffer and rapid spin columns to extract RNA from your isolated exosomes.

Choose the SeraMir Kit that’s right for you
Cat. #NameKit includes
ExoQuick or ExoQuick-TCSeraMir RNA columns and reagentsSeraMir cDNA synthesis and amplification reagents384-well plate miRNAs for human, mouse, or rat
RA800A-1Complete SeraMir Exosome RNA Amplification Kit 
RA800TC-1Complete SeraMir Exosome RNA Amplification Kit for Media and Urine 
RA806A-1SeraMir Exosome RNA Purification Kit  
RA806TC-1SeraMir Exosome RNA Purification Kit for Media and Urine  
RA808A-1SeraMir Exosome RNA Purification Column Kit   
RA820A-1Human Complete SeraMir Exosome RNA Amplification and Profiling Kit
RA820TC-1Human Complete SeraMir Exosome RNA Amplification and Profiling Kit for Media and Urine
RA821A-1Mouse Complete SeraMir Exosome RNA Amplification and Profiling Kit
RA821TC-1Mouse Complete SeraMir Exosome RNA Amplification and Profiling Kit for Media and Urine
RA822A-1Rat Complete SeraMir Exosome RNA Amplification and Profiling Kit
RA822TC-1Rat Complete SeraMir Exosome RNA Amplification and Profiling Kit for Media and Urine

How It Works

How It Works

Go from sample to amplified exoRNAs in a single day

  • Isolate exosomes from patient biofluids with the included ExoQuick reagent
  • Purify exoRNAs with SeraMir columns

Purified exoRNAs are fully compatible with most downstream applications, such as qPCR, microarray analysis, or NGS.

Isolate serum exosomes and purify exoRNAs

Streamline biomarker discovery with seramir – the workflow, steps 1 and 2

Tail exoRNAs and synthesize double-tagged cDNAStreamline biomarker discovery with seramir – the workflow, steps 3 and 4

Use the SeraMir spike-in RNA control in a qPCR assay to control for exoRNA recovery, tailing, and cDNA synthesis.Use the SeraMir spike-in RNA control in a qPCR assay to control for exoRNA recovery, tailing, and cDNA synthesis

Supporting Data

Supporting Data

Better qPCR profiling with SeraMir

SeraMir provides reliably better qPCR profiling than exosmal RNAs isolated using phenol/trizol

Figure 1. Serum RNA prepared by the SeraMir Kit delivers more reliable, reproducible qPCR profiles than when the RNA is isolated using conventional Trizol methods. Profiling of 380 Human microRNAs across the SeraMir 384 Profiler. The phenol-free exosome lysis step coupled to the small RNA binding columns isolates exoRNAs with much higher purity than Trizol/Phenol based methods. The SeraMir exoRNAs are compatible with downstream polyadenylation and reverse trancription reactions for amplification and accurate qPCR profiling.

 SeraMir provides reliably better qPCR profiling than exosmal RNAs isolated using phenol/trizol

Figure 2. Serum exoRNAs prepared using SeraMir deliver excellent performance in microarray studies. Samples from a pooled normal serum preparation and from a male caucasian (age 73) with adenocarcinoma of the colon were used in this study. Exosomes were precipitated from 250 µL of serum using the SeraMir Exosome RNA Amplification Kit. The T7-amplified “sense” exoRNAs were then used for direct labeling analyses on LC Sciences miRBase ver.16 array chips (performed in triplicate). The exoRNAs were hybridized across 1,214 different microRNAs on the probe set.
Of the 1,214 microRNAs analyzed, 79 microRNAs showed a signal intensity >32. Within this set of 79, there was a clear colon versus normal “signature set” of 40 microRNAs that could discriminate normal from colon cancer serum samples with a p-value < 0.01. The identities of the microRNAs found in this study have been masked while further investigation continues.

FAQs

Resources

Citations

  • Peng, Y, Huleihel, L & Cardenes, N. (2017) Different MiroRNA Expression In MSC-Derived Exosomes: IPF Patients And Age-Matched Normal Individuals. Conference. 2017;. Link: Conference
  • Sueta, A, et al. (2017) Abstract P1-02-13: Differential expression of exosomal miRNAs between breast cancer patients with recurrence and no-recurrence. Abstract. 2017;. Link: Abstract
  • Selmaj, I, et al. (2017) Global Exosome Transcriptome Profiling Reveals Biomarkers for Multiple Sclerosis. Ann. Neurol.. 2017;. PM ID: 28411393
  • Hubal, MJ, et al. (2017) Circulating adipocyte-derived exosomal MicroRNAs associated with decreased insulin resistance after gastric bypass. Obesity (Silver Spring). 2017; 25(1):102-110. PM ID: 27883272
  • Faust, A, et al. (2017) Urinary bladder extracellular matrix hydrogels and matrix-bound vesicles differentially regulate central nervous system neuron viability and axon growth and branching. J Biomater Appl. 2017; 31(9):1277-1295. PM ID: 28447547
  • Tsukita, S, et al. (2017) MicroRNAs 106b and 222 Improve Hyperglycemia in a Mouse Model of Insulin-Deficient Diabetes via Pancreatic β-Cell Proliferation. EBioMedicine. 2017; 15:163-172. PM ID: 27974246
  • Zhang, R, et al. (2017) Serum long non coding RNA MALAT-1 protected by exosomes is up-regulated and promotes cell proliferation and migration in non-small cell lung cancer. Biochem. Biophys. Res. Commun.. 2017;. PM ID: 28623135
  • Huleihel, L, et al. (2017) Matrix bound nanovesicles recapitulate extracellular matrix effects on macrophage phenotype. Tissue Eng Part A. 2017;. PM ID: 28580875
  • Holliday, LS, et al. (2017) Exosomes: novel regulators of bone remodelling and potential therapeutic agents for orthodontics. Orthod Craniofac Res. 2017;:95-99. PM ID: 28643924
  • Koyama, S, et al. (2017) Dynamic changes of serum microRNA-122-5p through therapeutic courses indicates amelioration of acute liver injury accompanied by acute cardiac decompensation. ESC Heart Fail. 2017; 4(2):112-121. PM ID: 28451447
  • Protocol, IIEI. (2017) List of Components. Product. 2017;. Link: Product
  • Josson, S, Gururajan, M & WKChung, L. (2017) miRNA Characterization from the Extracellular Vesicles. bio-protocol. 2017; 7(4). Link: bio-protocol
  • Nakano, T, et al. (2017) Hepatic miR-301a as a Liver Transplant Rejection Biomarker? And Its Role for Interleukin-6 Production in Hepatocytes. OMICS. 2017; 21(1):55-66. PM ID: 28271982
  • Zhou, X, et al. (2017) Characterization of mouse serum exosomal small RNA content: The origins and their roles in modulating inflammatory response. Oncotarget. 2017; 8(26):42712-42727. PM ID: 28514744
  • Val, S, et al. (2017) Purification and characterization of microRNAs within middle ear fluid exosomes: implication in otitis media pathophysiology. Pediatr. Res.. 2017;. PM ID: 28157838
  • Crossland, RE, et al. (2016) Evaluation of optimal extracellular vesicle small RNA isolation and qRT-PCR normalisation for serum and urine. J. Immunol. Methods. 2016; 429:39-49. PM ID: 26723490
  • Kudo, M. (2016) Speaker’s Lecture Abstracts. Liver Cancer. 2016; 5(1):1–94. Link: Liver Cancer
  • Clayton, A, et al. (2016) The 2nd United Kingdom Extracellular Vesicle Forum Meeting Abstracts: 15 December 2015, Hadyn Ellis Building, Cardiff University.. J Extracell Vesicles. 2016; 5:30924. PM ID: 26928673
  • Delić, D, et al. (2016) Urinary Exosomal miRNA Signature in Type II Diabetic Nephropathy Patients. PLoS ONE. 2016; 11(3):e0150154. PM ID: 26930277
  • Huang, Y, et al. (2016) 血清中外泌体及外泌体 RNA 提取方法的比较. 中华检验医学杂志. 2016; 39:427-432. Link: 中华检验医学杂志

Products

Catalog Number Description Size Price Quantity Add to Cart
RA806A-1 SeraMir Exosome RNA Purification kit (5 mL ExoQuick and 20 exoRNA columns) 20 Preps $511
- +

Overview

Overview

Optimized exosomal RNA extraction for biomarker discovery and more—for serum, plasma, and ascites fluid

Looking for biomarkers? Studying exosomal RNAs (exoRNAs)? Turn to SBI’s SeraMir Exosome RNA Purification Kit for optimized isolation of exoRNAs. The kit includes ExoQuick for efficient exosome isolation from serum, plasma, or ascites fluid, and SeraMir columns and reagents for purification of RNA from your isolated exosomes (for exoRNA isolation from other biofluids, try the SeraMir Exosome RNA Purification Kit for Media and Urine).

Reliable, reproducible exoRNA isolation from serum, plasma, or ascites fluid Find exosomal RNA biomarkers using the SeraMir Exosome RNA Amplification Kit

With cargo that reflects the makeup of their parent cells and their easy isolation, researchers are increasingly turning to exosomes as a source of disease-related biomarkers. To simplify and standardize the isolation of RNA from exosomes, SBI has developed the SeraMir family of products.

The SeraMir Exosome RNA Purification Kit includes everything you need for optimized isolation of exosomal RNAs from serum, plasma, or ascites fluid samples—ExoQuick for fast and efficient exosome isolation and a phenol-free lysis buffer and rapid spin columns to extract RNA from your isolated exosomes.

Choose the SeraMir Kit that’s right for you
Cat. #NameKit includes
ExoQuick or ExoQuick-TCSeraMir RNA columns and reagentsSeraMir cDNA synthesis and amplification reagents384-well plate miRNAs for human, mouse, or rat
RA800A-1Complete SeraMir Exosome RNA Amplification Kit 
RA800TC-1Complete SeraMir Exosome RNA Amplification Kit for Media and Urine 
RA806A-1SeraMir Exosome RNA Purification Kit  
RA806TC-1SeraMir Exosome RNA Purification Kit for Media and Urine  
RA808A-1SeraMir Exosome RNA Purification Column Kit   
RA820A-1Human Complete SeraMir Exosome RNA Amplification and Profiling Kit
RA820TC-1Human Complete SeraMir Exosome RNA Amplification and Profiling Kit for Media and Urine
RA821A-1Mouse Complete SeraMir Exosome RNA Amplification and Profiling Kit
RA821TC-1Mouse Complete SeraMir Exosome RNA Amplification and Profiling Kit for Media and Urine
RA822A-1Rat Complete SeraMir Exosome RNA Amplification and Profiling Kit
RA822TC-1Rat Complete SeraMir Exosome RNA Amplification and Profiling Kit for Media and Urine

How It Works

How It Works

Go from sample to amplified exoRNAs in a single day

  • Isolate exosomes from patient biofluids with the included ExoQuick reagent
  • Purify exoRNAs with SeraMir columns

Purified exoRNAs are fully compatible with most downstream applications, such as qPCR, microarray analysis, or NGS.

Isolate serum exosomes and purify exoRNAs

Streamline biomarker discovery with seramir – the workflow, steps 1 and 2

Tail exoRNAs and synthesize double-tagged cDNAStreamline biomarker discovery with seramir – the workflow, steps 3 and 4

Use the SeraMir spike-in RNA control in a qPCR assay to control for exoRNA recovery, tailing, and cDNA synthesis.Use the SeraMir spike-in RNA control in a qPCR assay to control for exoRNA recovery, tailing, and cDNA synthesis

Supporting Data

Supporting Data

Better qPCR profiling with SeraMir

SeraMir provides reliably better qPCR profiling than exosmal RNAs isolated using phenol/trizol

Figure 1. Serum RNA prepared by the SeraMir Kit delivers more reliable, reproducible qPCR profiles than when the RNA is isolated using conventional Trizol methods. Profiling of 380 Human microRNAs across the SeraMir 384 Profiler. The phenol-free exosome lysis step coupled to the small RNA binding columns isolates exoRNAs with much higher purity than Trizol/Phenol based methods. The SeraMir exoRNAs are compatible with downstream polyadenylation and reverse trancription reactions for amplification and accurate qPCR profiling.

 SeraMir provides reliably better qPCR profiling than exosmal RNAs isolated using phenol/trizol

Figure 2. Serum exoRNAs prepared using SeraMir deliver excellent performance in microarray studies. Samples from a pooled normal serum preparation and from a male caucasian (age 73) with adenocarcinoma of the colon were used in this study. Exosomes were precipitated from 250 µL of serum using the SeraMir Exosome RNA Amplification Kit. The T7-amplified “sense” exoRNAs were then used for direct labeling analyses on LC Sciences miRBase ver.16 array chips (performed in triplicate). The exoRNAs were hybridized across 1,214 different microRNAs on the probe set.
Of the 1,214 microRNAs analyzed, 79 microRNAs showed a signal intensity >32. Within this set of 79, there was a clear colon versus normal “signature set” of 40 microRNAs that could discriminate normal from colon cancer serum samples with a p-value < 0.01. The identities of the microRNAs found in this study have been masked while further investigation continues.

FAQs

Citations

  • Peng, Y, Huleihel, L & Cardenes, N. (2017) Different MiroRNA Expression In MSC-Derived Exosomes: IPF Patients And Age-Matched Normal Individuals. Conference. 2017;. Link: Conference
  • Sueta, A, et al. (2017) Abstract P1-02-13: Differential expression of exosomal miRNAs between breast cancer patients with recurrence and no-recurrence. Abstract. 2017;. Link: Abstract
  • Selmaj, I, et al. (2017) Global Exosome Transcriptome Profiling Reveals Biomarkers for Multiple Sclerosis. Ann. Neurol.. 2017;. PM ID: 28411393
  • Hubal, MJ, et al. (2017) Circulating adipocyte-derived exosomal MicroRNAs associated with decreased insulin resistance after gastric bypass. Obesity (Silver Spring). 2017; 25(1):102-110. PM ID: 27883272
  • Faust, A, et al. (2017) Urinary bladder extracellular matrix hydrogels and matrix-bound vesicles differentially regulate central nervous system neuron viability and axon growth and branching. J Biomater Appl. 2017; 31(9):1277-1295. PM ID: 28447547
  • Tsukita, S, et al. (2017) MicroRNAs 106b and 222 Improve Hyperglycemia in a Mouse Model of Insulin-Deficient Diabetes via Pancreatic β-Cell Proliferation. EBioMedicine. 2017; 15:163-172. PM ID: 27974246
  • Zhang, R, et al. (2017) Serum long non coding RNA MALAT-1 protected by exosomes is up-regulated and promotes cell proliferation and migration in non-small cell lung cancer. Biochem. Biophys. Res. Commun.. 2017;. PM ID: 28623135
  • Huleihel, L, et al. (2017) Matrix bound nanovesicles recapitulate extracellular matrix effects on macrophage phenotype. Tissue Eng Part A. 2017;. PM ID: 28580875
  • Holliday, LS, et al. (2017) Exosomes: novel regulators of bone remodelling and potential therapeutic agents for orthodontics. Orthod Craniofac Res. 2017;:95-99. PM ID: 28643924
  • Koyama, S, et al. (2017) Dynamic changes of serum microRNA-122-5p through therapeutic courses indicates amelioration of acute liver injury accompanied by acute cardiac decompensation. ESC Heart Fail. 2017; 4(2):112-121. PM ID: 28451447
  • Protocol, IIEI. (2017) List of Components. Product. 2017;. Link: Product
  • Josson, S, Gururajan, M & WKChung, L. (2017) miRNA Characterization from the Extracellular Vesicles. bio-protocol. 2017; 7(4). Link: bio-protocol
  • Nakano, T, et al. (2017) Hepatic miR-301a as a Liver Transplant Rejection Biomarker? And Its Role for Interleukin-6 Production in Hepatocytes. OMICS. 2017; 21(1):55-66. PM ID: 28271982
  • Zhou, X, et al. (2017) Characterization of mouse serum exosomal small RNA content: The origins and their roles in modulating inflammatory response. Oncotarget. 2017; 8(26):42712-42727. PM ID: 28514744
  • Val, S, et al. (2017) Purification and characterization of microRNAs within middle ear fluid exosomes: implication in otitis media pathophysiology. Pediatr. Res.. 2017;. PM ID: 28157838
  • Crossland, RE, et al. (2016) Evaluation of optimal extracellular vesicle small RNA isolation and qRT-PCR normalisation for serum and urine. J. Immunol. Methods. 2016; 429:39-49. PM ID: 26723490
  • Kudo, M. (2016) Speaker’s Lecture Abstracts. Liver Cancer. 2016; 5(1):1–94. Link: Liver Cancer
  • Clayton, A, et al. (2016) The 2nd United Kingdom Extracellular Vesicle Forum Meeting Abstracts: 15 December 2015, Hadyn Ellis Building, Cardiff University.. J Extracell Vesicles. 2016; 5:30924. PM ID: 26928673
  • Delić, D, et al. (2016) Urinary Exosomal miRNA Signature in Type II Diabetic Nephropathy Patients. PLoS ONE. 2016; 11(3):e0150154. PM ID: 26930277
  • Huang, Y, et al. (2016) 血清中外泌体及外泌体 RNA 提取方法的比较. 中华检验医学杂志. 2016; 39:427-432. Link: 中华检验医学杂志