Integrase-based Systems

When you want non-viral expression from low or single-copy integration events, and/or have a large insert size, choose one of SBI’s integrase-based systems

Choose one of SBI’s integrase-based gene expression systems when you want a non-viral system that integrates at low- or single-copy. Both our PhiC31 and PinPoint Systems support unlimited insert sizes, and come in a variety of promoter and reporter combinations.

The PhiC31 Integrase System

  • Frequently used for gene therapy development and related applications
  • Non-viral transgene delivery
  • Single-step process
  • Single-copy integration
  • Preferential integration at active sites
  • Delivery of inserts with no size constraints
  • Easy generation of cell lines

The PinPoint Targeted Integration System

  • Isogenic cell line creation
  • Reliable and efficient cell line generation and retargeting
  • Non-viral transgene delivery
  • Two-step process
  • High-efficiency, site-specific, single-copy integration
  • Delivery of inserts with no size constraints
  • Compatible with high-throughput workflows

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Integrase-based Systems

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PinPoint-FC 293T Platform Kit for Targeted Gene Insertion (PinPoint attP already placed)

Get your PinPoint projects going fast with this kit that includes 293T cells that already have a PinPoint attP site and all the necessary PinPoint plasmids
Size:
array(1) { [0]=> array(24) { ["attributes"]=> array(1) { ["attribute_size"]=> string(5) "1 Kit" } ["availability_html"]=> string(0) "" ["backorders_allowed"]=> bool(false) ["dimensions"]=> array(3) { ["length"]=> string(0) "" ["width"]=> string(0) "" ["height"]=> string(0) "" } ["dimensions_html"]=> string(3) "N/A" ["display_price"]=> float(4498) ["display_regular_price"]=> float(4498) ["image"]=> array(18) { ["title"]=> string(20) "pinpoint-293t-kit-01" ["caption"]=> string(0) "" ["url"]=> string(89) "https://systembio.com/wp-content/uploads/gene-expression-systems/pinpoint-293t-kit-01.png" ["alt"]=> string(0) "" ["src"]=> string(97) "https://systembio.com/wp-content/uploads/gene-expression-systems/pinpoint-293t-kit-01-600x600.png" ["srcset"]=> string(310) "https://systembio.com/wp-content/uploads/gene-expression-systems/pinpoint-293t-kit-01-600x600.png 600w, https://systembio.com/wp-content/uploads/gene-expression-systems/pinpoint-293t-kit-01-300x300.png 300w, https://systembio.com/wp-content/uploads/gene-expression-systems/pinpoint-293t-kit-01-100x100.png 100w" ["sizes"]=> string(31) "(max-width: 600px) 100vw, 600px" ["full_src"]=> string(89) "https://systembio.com/wp-content/uploads/gene-expression-systems/pinpoint-293t-kit-01.png" ["full_src_w"]=> int(2500) ["full_src_h"]=> int(2500) ["gallery_thumbnail_src"]=> string(97) "https://systembio.com/wp-content/uploads/gene-expression-systems/pinpoint-293t-kit-01-100x100.png" ["gallery_thumbnail_src_w"]=> int(100) ["gallery_thumbnail_src_h"]=> int(100) ["thumb_src"]=> string(97) "https://systembio.com/wp-content/uploads/gene-expression-systems/pinpoint-293t-kit-01-300x300.png" ["thumb_src_w"]=> int(300) ["thumb_src_h"]=> int(300) ["src_w"]=> int(600) ["src_h"]=> int(600) } ["image_id"]=> string(5) "11074" ["is_downloadable"]=> bool(false) ["is_in_stock"]=> bool(true) ["is_purchasable"]=> bool(true) ["is_sold_individually"]=> string(2) "no" ["is_virtual"]=> bool(false) ["max_qty"]=> string(0) "" ["min_qty"]=> int(1) ["price_html"]=> string(144) "$4,498" ["sku"]=> string(11) "PIN320A-KIT" ["variation_description"]=> string(124) "

PinPoint-FC 293T Platform Kit for Targeted Gene Insertion (includes PIN320A-1, PIN200A-1, PIN510A-1 & PIN600A-1)

" ["variation_id"]=> int(64630) ["variation_is_active"]=> bool(true) ["variation_is_visible"]=> bool(true) ["weight"]=> string(0) "" ["weight_html"]=> string(3) "N/A" } }
(Cat. # PIN320A-KIT)

PinPoint-FC Murine iPSC Platform Cell Line for Targeted Gene Insertion (PinPoint attP already placed)

Jump start your PinPoint cell line creation with this Murine iPS cell line that already has a single PinPoint attP site inserted into the genome
Size:
array(1) { [0]=> array(24) { ["attributes"]=> array(1) { ["attribute_size"]=> string(13) ">2x10^5 Cells" } ["availability_html"]=> string(0) "" ["backorders_allowed"]=> bool(false) ["dimensions"]=> array(3) { ["length"]=> string(0) "" ["width"]=> string(0) "" ["height"]=> string(0) "" } ["dimensions_html"]=> string(3) "N/A" ["display_price"]=> float(2809) ["display_regular_price"]=> float(2809) ["image"]=> array(18) { ["title"]=> string(42) "pinpoint-murine-ipsc-platform-cell-line-01" ["caption"]=> string(0) "" ["url"]=> string(111) "https://systembio.com/wp-content/uploads/gene-expression-systems/pinpoint-murine-ipsc-platform-cell-line-01.png" ["alt"]=> string(0) "" ["src"]=> string(119) "https://systembio.com/wp-content/uploads/gene-expression-systems/pinpoint-murine-ipsc-platform-cell-line-01-600x600.png" ["srcset"]=> string(376) "https://systembio.com/wp-content/uploads/gene-expression-systems/pinpoint-murine-ipsc-platform-cell-line-01-600x600.png 600w, https://systembio.com/wp-content/uploads/gene-expression-systems/pinpoint-murine-ipsc-platform-cell-line-01-300x300.png 300w, https://systembio.com/wp-content/uploads/gene-expression-systems/pinpoint-murine-ipsc-platform-cell-line-01-100x100.png 100w" ["sizes"]=> string(31) "(max-width: 600px) 100vw, 600px" ["full_src"]=> string(111) "https://systembio.com/wp-content/uploads/gene-expression-systems/pinpoint-murine-ipsc-platform-cell-line-01.png" ["full_src_w"]=> int(2500) ["full_src_h"]=> int(2500) ["gallery_thumbnail_src"]=> string(119) "https://systembio.com/wp-content/uploads/gene-expression-systems/pinpoint-murine-ipsc-platform-cell-line-01-100x100.png" ["gallery_thumbnail_src_w"]=> int(100) ["gallery_thumbnail_src_h"]=> int(100) ["thumb_src"]=> string(119) "https://systembio.com/wp-content/uploads/gene-expression-systems/pinpoint-murine-ipsc-platform-cell-line-01-300x300.png" ["thumb_src_w"]=> int(300) ["thumb_src_h"]=> int(300) ["src_w"]=> int(600) ["src_h"]=> int(600) } ["image_id"]=> string(5) "11081" ["is_downloadable"]=> bool(false) ["is_in_stock"]=> bool(true) ["is_purchasable"]=> bool(true) ["is_sold_individually"]=> string(2) "no" ["is_virtual"]=> bool(false) ["max_qty"]=> string(0) "" ["min_qty"]=> int(1) ["price_html"]=> string(144) "$2,809" ["sku"]=> string(11) "PIN340iPS-1" ["variation_description"]=> string(114) "

PinPoint-FC Murine iPSC Platform Cell Line for Targeted Gene Insertion (with PinPoint site already placed)

" ["variation_id"]=> int(64632) ["variation_is_active"]=> bool(true) ["variation_is_visible"]=> bool(true) ["weight"]=> string(0) "" ["weight_html"]=> string(3) "N/A" } }
(Cat. # PIN340iPS-1)

PinPoint-FC Murine iPSC Platform Kit for Targeted Gene Insertion (PinPoint attP already placed)

Get your PinPoint projects moving with this kit that includes Murine iPS cells that already have a PinPoint attP site and all the necessary PinPoint plasmids
Size:
array(1) { [0]=> array(24) { ["attributes"]=> array(1) { ["attribute_size"]=> string(5) "1 Kit" } ["availability_html"]=> string(0) "" ["backorders_allowed"]=> bool(false) ["dimensions"]=> array(3) { ["length"]=> string(0) "" ["width"]=> string(0) "" ["height"]=> string(0) "" } ["dimensions_html"]=> string(3) "N/A" ["display_price"]=> float(4498) ["display_regular_price"]=> float(4498) ["image"]=> array(18) { ["title"]=> string(27) "pinpoint-murine-ipsc-kit-01" ["caption"]=> string(0) "" ["url"]=> string(96) "https://systembio.com/wp-content/uploads/gene-expression-systems/pinpoint-murine-ipsc-kit-01.png" ["alt"]=> string(0) "" ["src"]=> string(104) "https://systembio.com/wp-content/uploads/gene-expression-systems/pinpoint-murine-ipsc-kit-01-600x600.png" ["srcset"]=> string(331) "https://systembio.com/wp-content/uploads/gene-expression-systems/pinpoint-murine-ipsc-kit-01-600x600.png 600w, https://systembio.com/wp-content/uploads/gene-expression-systems/pinpoint-murine-ipsc-kit-01-300x300.png 300w, https://systembio.com/wp-content/uploads/gene-expression-systems/pinpoint-murine-ipsc-kit-01-100x100.png 100w" ["sizes"]=> string(31) "(max-width: 600px) 100vw, 600px" ["full_src"]=> string(96) "https://systembio.com/wp-content/uploads/gene-expression-systems/pinpoint-murine-ipsc-kit-01.png" ["full_src_w"]=> int(2500) ["full_src_h"]=> int(2500) ["gallery_thumbnail_src"]=> string(104) "https://systembio.com/wp-content/uploads/gene-expression-systems/pinpoint-murine-ipsc-kit-01-100x100.png" ["gallery_thumbnail_src_w"]=> int(100) ["gallery_thumbnail_src_h"]=> int(100) ["thumb_src"]=> string(104) "https://systembio.com/wp-content/uploads/gene-expression-systems/pinpoint-murine-ipsc-kit-01-300x300.png" ["thumb_src_w"]=> int(300) ["thumb_src_h"]=> int(300) ["src_w"]=> int(600) ["src_h"]=> int(600) } ["image_id"]=> string(5) "11183" ["is_downloadable"]=> bool(false) ["is_in_stock"]=> bool(true) ["is_purchasable"]=> bool(true) ["is_sold_individually"]=> string(2) "no" ["is_virtual"]=> bool(false) ["max_qty"]=> string(0) "" ["min_qty"]=> int(1) ["price_html"]=> string(144) "$4,498" ["sku"]=> string(13) "PIN340iPS-KIT" ["variation_description"]=> string(133) "

PinPoint-FC Murine iPSC Platform Kit for Targeted Gene Insertion (includes PIN340iPS-1, PIN200A-1, PIN510A-1 & PIN600A-1)

" ["variation_id"]=> int(64634) ["variation_is_active"]=> bool(true) ["variation_is_visible"]=> bool(true) ["weight"]=> string(0) "" ["weight_html"]=> string(3) "N/A" } }
(Cat. # PIN340iPS-KIT)

PinPoint-HR attP Placement Vector with MCS

Place a PinPoint attP site into a specific location in the target genome with the CRISPR/Cas9 System and this PinPoint HR Targeting Vector
Size:
array(1) { [0]=> array(24) { ["attributes"]=> array(1) { ["attribute_size"]=> string(6) "10 µg" } ["availability_html"]=> string(0) "" ["backorders_allowed"]=> bool(false) ["dimensions"]=> array(3) { ["length"]=> string(0) "" ["width"]=> string(0) "" ["height"]=> string(0) "" } ["dimensions_html"]=> string(3) "N/A" ["display_price"]=> float(1120) ["display_regular_price"]=> float(1120) ["image"]=> array(18) { ["title"]=> string(45) "pinpoint-hr-attp-placement-vector-with-mcs-01" ["caption"]=> string(0) "" ["url"]=> string(114) "https://systembio.com/wp-content/uploads/gene-expression-systems/pinpoint-hr-attp-placement-vector-with-mcs-01.png" ["alt"]=> string(0) "" ["src"]=> string(122) "https://systembio.com/wp-content/uploads/gene-expression-systems/pinpoint-hr-attp-placement-vector-with-mcs-01-600x600.png" ["srcset"]=> string(385) "https://systembio.com/wp-content/uploads/gene-expression-systems/pinpoint-hr-attp-placement-vector-with-mcs-01-600x600.png 600w, https://systembio.com/wp-content/uploads/gene-expression-systems/pinpoint-hr-attp-placement-vector-with-mcs-01-300x300.png 300w, https://systembio.com/wp-content/uploads/gene-expression-systems/pinpoint-hr-attp-placement-vector-with-mcs-01-100x100.png 100w" ["sizes"]=> string(31) "(max-width: 600px) 100vw, 600px" ["full_src"]=> string(114) "https://systembio.com/wp-content/uploads/gene-expression-systems/pinpoint-hr-attp-placement-vector-with-mcs-01.png" ["full_src_w"]=> int(2500) ["full_src_h"]=> int(2500) ["gallery_thumbnail_src"]=> string(122) "https://systembio.com/wp-content/uploads/gene-expression-systems/pinpoint-hr-attp-placement-vector-with-mcs-01-100x100.png" ["gallery_thumbnail_src_w"]=> int(100) ["gallery_thumbnail_src_h"]=> int(100) ["thumb_src"]=> string(122) "https://systembio.com/wp-content/uploads/gene-expression-systems/pinpoint-hr-attp-placement-vector-with-mcs-01-300x300.png" ["thumb_src_w"]=> int(300) ["thumb_src_h"]=> int(300) ["src_w"]=> int(600) ["src_h"]=> int(600) } ["image_id"]=> string(5) "11147" ["is_downloadable"]=> bool(false) ["is_in_stock"]=> bool(true) ["is_purchasable"]=> bool(true) ["is_sold_individually"]=> string(2) "no" ["is_virtual"]=> bool(false) ["max_qty"]=> string(0) "" ["min_qty"]=> int(1) ["price_html"]=> string(144) "$1,120" ["sku"]=> string(9) "PIN400A-1" ["variation_description"]=> string(75) "

PinPoint-HR attP Placement Vector – Empty MCS for cloning HR Arms

" ["variation_id"]=> int(64636) ["variation_is_active"]=> bool(true) ["variation_is_visible"]=> bool(true) ["weight"]=> string(0) "" ["weight_html"]=> string(3) "N/A" } }
(Cat. # PIN400A-1)

PinPoint-HR System for Platform Cell Line Generation & Retargeting

Get all the PinPoint plasmids you need for PinPoint Targeted Integration using the CRISPR/Cas9 System to place the PinPoint attP site into the target genome
Size:
array(1) { [0]=> array(24) { ["attributes"]=> array(1) { ["attribute_size"]=> string(5) "1 Kit" } ["availability_html"]=> string(0) "" ["backorders_allowed"]=> bool(false) ["dimensions"]=> array(3) { ["length"]=> string(0) "" ["width"]=> string(0) "" ["height"]=> string(0) "" } ["dimensions_html"]=> string(3) "N/A" ["display_price"]=> float(2527) ["display_regular_price"]=> float(2527) ["image"]=> array(18) { ["title"]=> string(21) "pinpoint-hr-system-01" ["caption"]=> string(0) "" ["url"]=> string(90) "https://systembio.com/wp-content/uploads/gene-expression-systems/pinpoint-hr-system-01.png" ["alt"]=> string(0) "" ["src"]=> string(98) "https://systembio.com/wp-content/uploads/gene-expression-systems/pinpoint-hr-system-01-600x600.png" ["srcset"]=> string(313) "https://systembio.com/wp-content/uploads/gene-expression-systems/pinpoint-hr-system-01-600x600.png 600w, https://systembio.com/wp-content/uploads/gene-expression-systems/pinpoint-hr-system-01-300x300.png 300w, https://systembio.com/wp-content/uploads/gene-expression-systems/pinpoint-hr-system-01-100x100.png 100w" ["sizes"]=> string(31) "(max-width: 600px) 100vw, 600px" ["full_src"]=> string(90) "https://systembio.com/wp-content/uploads/gene-expression-systems/pinpoint-hr-system-01.png" ["full_src_w"]=> int(2500) ["full_src_h"]=> int(2500) ["gallery_thumbnail_src"]=> string(98) "https://systembio.com/wp-content/uploads/gene-expression-systems/pinpoint-hr-system-01-100x100.png" ["gallery_thumbnail_src_w"]=> int(100) ["gallery_thumbnail_src_h"]=> int(100) ["thumb_src"]=> string(98) "https://systembio.com/wp-content/uploads/gene-expression-systems/pinpoint-hr-system-01-300x300.png" ["thumb_src_w"]=> int(300) ["thumb_src_h"]=> int(300) ["src_w"]=> int(600) ["src_h"]=> int(600) } ["image_id"]=> string(5) "11138" ["is_downloadable"]=> bool(false) ["is_in_stock"]=> bool(true) ["is_purchasable"]=> bool(true) ["is_sold_individually"]=> string(2) "no" ["is_virtual"]=> bool(false) ["max_qty"]=> string(0) "" ["min_qty"]=> int(1) ["price_html"]=> string(144) "$2,527" ["sku"]=> string(11) "PIN400A-KIT" ["variation_description"]=> string(138) "

PinPoint-HR System for Platform Cell Line Generation & Retargeting (includes PIN400A-1, PIN200A-1, PIN510A-1, & PIN600A-1)

" ["variation_id"]=> int(64638) ["variation_is_active"]=> bool(true) ["variation_is_visible"]=> bool(true) ["weight"]=> string(0) "" ["weight_html"]=> string(3) "N/A" } }
(Cat. # PIN400A-KIT)

PinPoint-HR attP Placement Vector Targeting the AAVS1 Safe Harbor Site

Place a PinPoint attP site into the AAVS1 Safe Harbor Site in the target genome with the CRISPR/Cas9 System and this PinPoint HR Targeting Vector
Size:
array(1) { [0]=> array(24) { ["attributes"]=> array(1) { ["attribute_size"]=> string(6) "10 µg" } ["availability_html"]=> string(0) "" ["backorders_allowed"]=> bool(false) ["dimensions"]=> array(3) { ["length"]=> string(0) "" ["width"]=> string(0) "" ["height"]=> string(0) "" } ["dimensions_html"]=> string(3) "N/A" ["display_price"]=> float(1120) ["display_regular_price"]=> float(1120) ["image"]=> array(18) { ["title"]=> string(52) "pinpoint-hr-attp-placement-vector-targeting-aavs1-01" ["caption"]=> string(0) "" ["url"]=> string(121) "https://systembio.com/wp-content/uploads/gene-expression-systems/pinpoint-hr-attp-placement-vector-targeting-aavs1-01.png" ["alt"]=> string(0) "" ["src"]=> string(129) "https://systembio.com/wp-content/uploads/gene-expression-systems/pinpoint-hr-attp-placement-vector-targeting-aavs1-01-600x600.png" ["srcset"]=> string(406) "https://systembio.com/wp-content/uploads/gene-expression-systems/pinpoint-hr-attp-placement-vector-targeting-aavs1-01-600x600.png 600w, https://systembio.com/wp-content/uploads/gene-expression-systems/pinpoint-hr-attp-placement-vector-targeting-aavs1-01-300x300.png 300w, https://systembio.com/wp-content/uploads/gene-expression-systems/pinpoint-hr-attp-placement-vector-targeting-aavs1-01-100x100.png 100w" ["sizes"]=> string(31) "(max-width: 600px) 100vw, 600px" ["full_src"]=> string(121) "https://systembio.com/wp-content/uploads/gene-expression-systems/pinpoint-hr-attp-placement-vector-targeting-aavs1-01.png" ["full_src_w"]=> int(2500) ["full_src_h"]=> int(2500) ["gallery_thumbnail_src"]=> string(129) "https://systembio.com/wp-content/uploads/gene-expression-systems/pinpoint-hr-attp-placement-vector-targeting-aavs1-01-100x100.png" ["gallery_thumbnail_src_w"]=> int(100) ["gallery_thumbnail_src_h"]=> int(100) ["thumb_src"]=> string(129) "https://systembio.com/wp-content/uploads/gene-expression-systems/pinpoint-hr-attp-placement-vector-targeting-aavs1-01-300x300.png" ["thumb_src_w"]=> int(300) ["thumb_src_h"]=> int(300) ["src_w"]=> int(600) ["src_h"]=> int(600) } ["image_id"]=> string(5) "10993" ["is_downloadable"]=> bool(false) ["is_in_stock"]=> bool(true) ["is_purchasable"]=> bool(true) ["is_sold_individually"]=> string(2) "no" ["is_virtual"]=> bool(false) ["max_qty"]=> string(0) "" ["min_qty"]=> int(1) ["price_html"]=> string(144) "$1,120" ["sku"]=> string(9) "PIN410A-1" ["variation_description"]=> string(87) "

PinPoint-HR attP Placement Vector – for targeting the Human AAVS1 Safe Harbor

" ["variation_id"]=> int(64640) ["variation_is_active"]=> bool(true) ["variation_is_visible"]=> bool(true) ["weight"]=> string(0) "" ["weight_html"]=> string(3) "N/A" } }
(Cat. # PIN410A-1)

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