pCDH-EF1α-MCS-T2A-RFP (PGK-Puro) Bidirectional Promoter Cloning and Expression Lentivector

Get another option for robust, coordinated dual gene expression with the pCDH-EF1α-MCS-T2A-RFP (PGK-Puro) Bidirectional Promoter Lentivector

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pCDH-EF1α-MCS-T2A-RFP (PGK-Puro) Cloning and Expression Lentivector

10 µg
CD822A-1
$ 532
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Overview

Get robust, stable expression of your gene-of-interest in dividing and quiescent cells

Well-regarded in the industry for reliable gene expression, SBI’s lentiviral vectors come in a variety of formats that support a wide range of applications. The pCDH-EF1α-MCS-T2A-RFP (PGK-Puro) Bidirectional Promoter Cloning and Expression Lentivector gives you another option for robust, coordinated expression of your gene-of-interest with two different markers. Co-express your gene-of-interest and RFP via a T2A element, and divergently express a puromycin resistance marker from the PGK promoter.

This promoter configuration takes advantage of the natural bidirectional activity of the PGK promoter, and this divergent configuration is both stable and resistant to silencing in embryonic stem (ES) cells, trophectoderm stem (TS) cells, and extra embryonic endoderm (XEN) stem cells1.
In addition, SBI’s Bidirectional Promoter Cloning and Expression Lentivectors enable:

  • Coordinate expression of your gene-of-interest with a marker without the use of IRES elements, which can provide weak co-expression of two cDNAs
  • Avoidance of unidirectional promoter interference issues that can sometimes arise when using lentivectors

pCDH-EF1α-MCS-T2A-RFP (PGK-Puro) Bidirectional Promoter Cloning and Expression Lentivector

With over thirty HIV-based lentivector options for gene expression, SBI’s family of pCDH Cloning and Expression Lentivectors provide the right lentivector for almost any gene expression project:

  • Multiple promoter options
  • Multiple marker options—Puro, Neo, Hygro, GFP, or RFP
  • Coordinated co-expression via T2A or IRES
  • Single promoter, dual promoter, and bidirectional promoter formats
  • High expression in most hematopoietic, embryonic, and stem cells via the MSCV CpG-deficient promoter

Not sure which promoter is right for you? This table should help:

Promoter Expression Level Application
CMV High Commonly used in most cell lines (HeLa, HEK293, HT1080, etc.)
MSCV High Hematopoietic and stem cells
EF1α Medium Most cell types including primary cells and stem cells
PGK Medium Most cell types including primary cells and stem cells
UbC Low Most cell types including primary cells and stem cells

References

  1. Golding MC and Mann MR. A bidirectional promoter architecture enhances lentiviral transgenesis in embryonic and extraembryonic stem cells. Gene Ther. 2011 Aug; 18(8):817-26. PMID: 21390068.

Supporting Data

Express multiple transgenes simultaneously with SBI’s Bidirectional Promoter Lentivectors

Express multiple transgenes simultaneously with SBI’s Bidirectional Promoter Lentivectors.

Sample data showing effective transduction with SBI’s Bidirectional Promoter Lentivectors. HEK293 cells were transduced with pCDH-EF1α-MCS-(PGK-Puro) (Cat.# CDH810A-1, upper left panel), pCDH-EF1α-MCS-(PGK-GFP-T2A-Puro) (Cat.# CDH813A-1, upper right panel), pCDH-EF1α-MCS-T2A-RFP (PGK-Puro) (Cat.# CDH822A-1, bottom left panel), pCDH-EF1α-MCS-T2A-GFP (PGK-Puro) (Cat.# CDH823A-1, bottom right panel). After four days, cells were imaged—the high number of cells present after puromycin selection (upper left panel) and cells showing GFP or RFP signal (all other panels) demonstrates the effectiveness of this lentivector family.


Citations

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