PB-EF1α-Oct4-Sox2-Klf4-Myc-IRES-GFP Human 4-in-1 iPSC PiggyBac Vector

Take advantage of the large insert capabilities of the PiggyBac Transposon System to simultaneously deliver four human reprogramming factors for iPSC generation

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PB-EF1α-Oct4-Sox2-Klf4-Myc-IRES-GFP Human 4-in-1 iPSC Vector

10 µg
PB630A-1
$ 1458
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Overview

Easy transgenesis means easy reprogramming

With the large insert capabilities of the PiggyBac Transposon System and the system’s easy, consistent transgenesis, you can use a single vector—the PB-EF1α-Oct4-Sox2-Klf4-Myc-IRES-GFP Human 4-in-1 iPSC PiggyBac Vector—to generate human iPSCs. This pre-built vector is ready-to-co-transfect with the Super PiggyBac Transposase Expression Vector (Cat.# PB210PA-1), and delivers human Oct4, Sox2, Klf4, and Myc co-expressed from a moderate EF1α promoter. The vector also includes a GFP reporter to simplify selection of transfectants.

PB-EF1α-Oct4-Sox2-Klf4-Myc-IRES-GFP Human 4-in-1 iPSC PiggyBac Vector

With the PiggyBac Transposon System, you can:

  • Make transgenic cell lines with a single transfection
  • Integrate multiple PiggyBac Vectors in a single transfection
  • Insert an expression cassette into human, mouse, and rat cells
  • Deliver virtually any-sized DNA insert, from 10 – 100 kb
  • Choose from PiggyBac Vectors that express your gene-of-interest from constitutive or inducible promoters and include a variety of markers
  • Determine the number of integration events with the PiggyBac qPCR Copy Number Kit (# PBC100A-1)

Customer Agreements
Academic customers can purchase PiggyBac Transposon System components for internal research purposes for indefinite use, whereas commercial customers must sign a customer agreement for a six-month, limited-use license to test the technology.
For end user license information, see the following:

* SBI is fully licensed to distribute PiggyBac vectors as a partnership with Transposagen Biopharmaceuticals, Inc.

How It Works

The PiggyBac Transposon System’s Cut-and-Paste Mechanism

The efficient PiggyBac Transposon System uses a cut-and-paste mechanism to transfer DNA from the PiggyBac Vector into the genome. If only temporary genomic integration is desired, the Excision-only PiggyBac Transposase can be transiently expressed for footprint-free removal of the insert, resulting in reconstitution of the original genome sequence.

The PiggyBac Transposon System’s cut-and-paste mechanism

Figure 1. The PiggyBac Transposon System’s cut-and-paste mechanism.

  • The Super PiggyBac Transposase binds to specific inverted terminal repeats (ITRs) in the PiggyBac Cloning and Expression Vector and excises the ITRs and intervening DNA.
  • The Super PiggyBac Transposase inserts the ITR-Expression Cassette-ITR segment into the genome at TTAA sites.
  • The Excision-only Super PiggyBac Transposase can be used to remove the ITR-Expression Cassette-ITR segment from the genome, for footprint-free removal

Supporting Data

Easily reprogram human cells with the PiggyBac Transposon System

Easily reprogram human cells with the PiggyBac Transposon System

Figure 2. Easily reprogram human cells with the PiggyBac Transposon System. Human neonatal skin fibroblasts were co-transfected with PB-EF1α-Oct4-Sox2-Klf4-Myc-IRES-GFP Human 4-in-1 iPSC PiggyBac Vector (Cat.# PB630A-1) and the Super PiggyBac Transposase Expression Vector (Cat.# PB210PA-1). iPS cells were derived using morphological selection criteria. When cultured under standard human ES cell culture conditions, the morphology of the custom human iPS cells was identical to that of human ES cells. Additionally, the cells express the pluripotency markers Oct4, Nanog, SSEA3, TRA-1-60, and demonstrate strong endogenous alkaline phosphatase staining.


Citations

  • Schertzer, MD, et al. (2019) lncRNA-Induced Spread of Polycomb Controlled by Genome Architecture, RNA Abundance, and CpG Island DNA. Mol. Cell. 2019 Jun 27;. PM ID: 31256989
  • Denes, LT, et al. (2019) Culturing C2C12 myotubes on micromolded gelatin hydrogels accelerates myotube maturation. Skelet Muscle. 2019 Jun 7; 9(1):17. PM ID: 31174599
  • Richter, JF, et al. (2019) Occludin knockdown is not sufficient to induce transepithelial macromolecule passage. Tissue Barriers. 2019 Jun 4; 7(2):1612661. PM ID: 31161924
  • Mao, X, et al. (2019) Schedule-dependent potentiation of chemotherapy drugs by the hypoxia-activated prodrug SN30000. Cancer Biol. Ther.. 2019 May 26;:1-12. PM ID: 31131698
  • Chapnick, DA, et al. (2019) Temporal Metabolite, Ion, and Enzyme Activity Profiling Using Fluorescence Microscopy and Genetically Encoded Biosensors. Methods Mol. Biol.. 2019 May 24; 1978:343-353. PM ID: 31119673
  • Shinmura, K, et al. (2019) POLQ Overexpression Is Associated with an Increased Somatic Mutation Load and PLK4 Overexpression in Lung Adenocarcinoma. Cancers (Basel). 2019 May 24; 11(5). PM ID: 31137743
  • Li, F, et al. (2019) A piggyBac-based TANGO GFP assay for high throughput screening of GPCR ligands in live cells. Cell Commun. Signal. 2019 May 23; 17(1):49. PM ID: 31122241
  • Shrestha, M, et al. (2019) The transition of tissue inhibitor of metalloproteinases from -4 to -1 induces aggressive behavior and poor patient survival in dedifferentiated liposarcoma via YAP/TAZ activation. Carcinogenesis. 2019 May 10;. PM ID: 31074490
  • Gan, L, et al. (2019) The lysosomal GPCR-like protein GPR137B regulates Rag and mTORC1 localization and activity. Nat. Cell Biol.. 2019 May 1; 21(5):614-626. PM ID: 31036939
  • Ahmad, ST, et al. (2019) Capicua regulates neural stem cell proliferation and lineage specification through control of Ets factors. Nat Commun. 2019 May 1; 10(1):2000. PM ID: 31043608
  • Rivera, FJ, et al. (2019) Aging restricts the ability of mesenchymal stem cells to promote the generation of oligodendrocytes during remyelination. Glia. 2019 Apr 30;. PM ID: 31038798
  • Inoue, M, et al. (2019) Structural Basis of Sarco/Endoplasmic Reticulum Ca2+-ATPase 2b Regulation via Transmembrane Helix Interplay. Cell Rep. 2019 Apr 23; 27(4):1221-1230.e3. PM ID: 31018135
  • Bielczyk-Maczyńska, E, et al. (2019) Loss of adipocyte identity through synergistic repression of PPARγ by TGF-β and mechanical stress. bioRxiv. 2019 Apr 11;. Link: bioRxiv
  • Paydarnia, N, et al. (2019) Synergistic effect of granzyme B-azurin fusion protein on breast cancer cells. Mol. Biol. Rep.. 2019 Apr 1;. PM ID: 30937652
  • Sakahara, M, et al. (2019) IFN/STAT signaling controls tumorigenesis and the drug response in colorectal cancer. Cancer Sci.. 2019 Apr 1; 110(4):1293-1305. PM ID: 30724425
  • Jones, EM, et al. (2019) A Scalable, Multiplexed Assay for Decoding GPCR-Ligand Interactions with RNA Sequencing. Cell Syst. 2019 Mar 27; 8(3):254-260.e6. PM ID: 30904378
  • Sauter, EJ, et al. (2019) Induced Neurons for the Study of Neurodegenerative and Neurodevelopmental Disorders. Methods Mol. Biol.. 2019 Mar 23; 1942:101-121. PM ID: 30900179
  • Laugsch, M, et al. (2019) Modeling the Pathological Long-Range Regulatory Effects of Human Structural Variation with Patient-Specific hiPSCs. Cell Stem Cell. 2019 Mar 21;. PM ID: 30982769
  • Farhadi, A, et al. (2019) Ultrasound Imaging of Gene Expression in Mammalian Cells. bioRxiv. 2019 Mar 18;. Link: bioRxiv
  • Garivet, G, et al. (2019) Small-Molecule Inhibition of the UNC-Src Interaction Impairs Dynamic Src Localization in Cells. Cell Chem Biol. 2019 Mar 13;. PM ID: 30956149