pGreenFire 2.0 NFκB Reporter Lentivector & Virus

Study NF-κB signaling with the pGreenFire 2.0 NFκB Reporter (red firefly luciferase & GFP), which is engineered for reliable stable cell line generation.

Description
Size
Catalog Number
Price
Quantity
Add to Cart

pGreenFire 2.0 NFkB reporter plasmid (pGF2-NFκB-rFluc-T2A-GFP-mPGK-Puro)

10 µg
TR412PA-P
$ 660

pGreenFire 2.0 NFkB reporter virus (pGF2-NFκB-rFluc-T2A-GFP-mPGK-Puro)

>2 x10^6 IFUs
TR412VA-P
$ 660
Contact Us Speak to a specialist
1-888-266-5066

Overview

Monitor signal transduction in real time with our re-engineered pGreenFire 2.0 Lentivectors

SBI has upgraded our popular pGreenFire signaling pathway reporter lentivectors with a design that leads to more reliable generation of stable cell lines. We’ve also swapped in the red firefly luciferase reporter (rFLuc), which opens up the possibility of performing a dual-spectral luciferase assay and delivers greater sensitivity for in vivo applications than conventional luciferase.

With the pGreenFire 2.0 NFκB Reporter Lentivector & Virus (pGF2-NFκB-rFluc-T2A-GFP-mPGK-Puro), the core reporter functionality is similar to the original pGreenFire lentivector—NF-κB transcriptional response elements (TREs) are placed upstream of a minimal CMV promoter (mCMV) which together drive co-expression of rFLuc and GFP in response to NF-κB activity. The result is the ability to quantitatively measure NF-κB activity using both fluorescence and luciferase activity.

What makes our next-gen pGreenFire 2.0 vectors even better than other TRE reporter vectors is the smart design, which adds in a constitutive selection cassette for stable cell line generation while minimizing interference with the upstream TRE. By using a weak/moderate mPGK promoter to drive the antibiotic selection marker (puromycin resistance) and carefully arranging the conditional reporter genes, the selection marker is reliably expressed without compromising conditional expression of rFLuc and GFP.

As with our original pGreenFire1 vectors, all pGreenFire 2.0 lentivectors leverage our reliable lentivector technology and save you time with pre-built signal transduction pathway reporters that come as ready-to-transduce pre-packaged lentivirus and plasmid that can be transfected into the lentivirus producing system of your choice*.

  • Sort responsive cells with GFP
  • Measure activity with red firefly luciferase
  • Leverage SBI’s highly-regarded lentivectors
  • Create stable signaling pathway reporter cell lines
  • Introduce reporters into difficult-to-transfect cell types, including primary and non-dividing mammalian cell lines

 pGreenFire 2.0 NFκB Reporter Lentivector & Virus

*Please note that these vectors only function properly when transduced. Transfection keeps the constitutive RSV promoter intact, leading to nonspecific expression of the reporter genes.

Supporting Data

See our pGreenFire 2.0 transcriptional response element reporters in action

The pGreenFire 2.0 NFκB Reporter efficiently and quantitatively reports on NFκB activity in MDA-MB-213 cells

Figure 1. The pGreenFire 2.0 NFκB Reporter efficiently and quantitatively reports on NFκB activity in MDA-MB-213 cells. Relative luciferase activity (A) and GFP activity (B) both increase in response to TNFα, and NFκB inducer. (C) Like all pGreenFire 2.0 lentivectors, the pGreenFire 2.0 NFκB Reporter contains an mPGK-Puro cassette to streamline creation of stable reporters integrated into the cell lines of your choice.

Citations

  • Winston, C, et al. (2019) Complement protein levels in plasma astrocyte-derived exosomes are abnormal in conversion from mild cognitive impairment to Alzheimer’s disease dementia. Alzheimer's & Dementia: Diagnosis, Assessment & Disease Monitoring. 2019 Dec 1; 11:61-66. Link: Alzheimer's & Dementia: Diagnosis, Assessment & Disease Monitoring
  • Kim, Y & Mok, H. (2019) Citraconylated exosomes for improved internalization into macrophages. Appl Biol Chem. 2019 Dec 1; 62(1). Link: Appl Biol Chem
  • Saunders, CA, et al. (2019) Genetic manipulation of PLB-985 cells and quantification of chemotaxis using the underagarose assay. Methods Cell Biol.. 2019 Oct 29; 149:31-56. PM ID: 30616826
  • Sekiba, K, et al. (2019) Inhibition of HBV Transcription From cccDNA With Nitazoxanide by Targeting the HBx-DDB1 Interaction. Cell Mol Gastroenterol Hepatol. 2019 Oct 24; 7(2):297-312. PM ID: 30704981
  • Yang, JS, et al. (2019) Investigation of lipidomic perturbations in oxidatively stressed subcellular organelles and exosomes by asymmetrical flow field-flow fractionation and nanoflow ultrahigh performance liquid chromatography-tandem mass spectrometry. Anal. Chim. Acta. 2019 Sep 27; 1073:79-89. PM ID: 31146839
  • Ishihara, R, et al. (2019) Design of a surface-functionalized power-free microchip for extracellular vesicle detection utilizing UV grafting. Reactive and Functional Polymers. 2019 Sep 1; 142:183-188. Link: Reactive and Functional Polymers
  • Gamboa, L, et al. (2019) Heat-triggered remote control of CRISPR-dCas9 for tunable transcriptional modulation. bioRxiv. 2019 Aug 14;. Link: bioRxiv
  • Liu, L, et al. (2019) IL‑17A promotes CXCR2‑dependent angiogenesis in a mouse model of liver cancer. Mol Med Rep. 2019 Aug 1; 20(2):1065-1074. PM ID: 31173199
  • Xu, Q, et al. (2019) miR‑132 inhibits high glucose‑induced vascular smooth muscle cell proliferation and migration by targeting E2F5. Mol Med Rep. 2019 Aug 1; 20(2):2012-2020. PM ID: 31257477
  • Hisano, Y, et al. (2019) Lysolipid receptor cross-talk regulates lymphatic endothelial junctions in lymph nodes. J. Exp. Med.. 2019 Jul 1; 216(7):1582-1598. PM ID: 31147448
  • Choi, YW, et al. (2019) Abrogation of B-RafV600E induced senescence by FoxM1 expression. Biochem. Biophys. Res. Commun.. 2019 Jun 30;. PM ID: 31270027
  • Madhu, LN, et al. (2019) Neuroinflammation in Gulf War Illness is linked with HMGB1 and complement activation, which can be discerned from brain-derived extracellular vesicles in the blood. Brain Behav. Immun.. 2019 Jun 27;. PM ID: 31255677
  • Amado, F, et al. (2019) Sample Treatment for Saliva Proteomics. Adv. Exp. Med. Biol.. 2019 Jun 26; 1073:23-56. PM ID: 31236838
  • Tian, L, et al. (2019) Long-read sequencing unveils IGH-DUX4 translocation into the silenced IGH allele in B-cell acute lymphoblastic leukemia. Nat Commun. 2019 Jun 26; 10(1):2789. PM ID: 31243274
  • Chen, CY, et al. (2019) Extracellular vesicles from human urine-derived stem cells prevent osteoporosis by transferring CTHRC1 and OPG. Bone Res. 2019 Jun 26; 7:18. PM ID: 31263627
  • Zhang, L & Li, D. (2019) MORC2 regulates DNA damage response through a PARP1-dependent pathway. Nucleic Acids Research. 2019 Jun 25;. Link: Nucleic Acids Research
  • Lee, H, et al. (2019) Caveolin-1 selectively regulates microRNA sorting into microvesicles after noxious stimuli. J. Exp. Med.. 2019 Jun 24;. PM ID: 31235510
  • Ariyoshi, K, et al. (2019) Radiation-Induced Bystander Effect is Mediated by Mitochondrial DNA in Exosome-Like Vesicles. Sci Rep. 2019 Jun 24; 9(1):9103. PM ID: 31235776
  • Zhao, J, et al. (2019) Tumor-Derived Extracellular Vesicles Inhibit Natural Killer Cell Function in Pancreatic Cancer. Cancers (Basel). 2019 Jun 22; 11(6). PM ID: 31234517
  • Wang, X, et al. (2019) Prostate carcinoma cell-derived exosomal MicroRNA-26a modulates the metastasis and tumor growth of prostate carcinoma. Biomed. Pharmacother.. 2019 Jun 20; 117:109109. PM ID: 31229922