Overview
Easily study the dynamics of cellular processes with SBI’s Cyto-Tracers™
Leverage SBI’s high-quality lentivector technology to study subcellular localization and dynamics with our line of Cyto-Tracer vectors. Easily create stable reporter cell lines with our validated, pre-built lentivectors—available as transfectable plasmids or ready-to-transduce, pre-packaged virus. The Cytosol Cyto-Tracer, pCT-Cyto-GFP (CMV), expresses an untagged copGFP, which naturally localizes to the cytosol. copGFP is expressed from the CMV promoter for strong expression in common cell types such as HeLa, HEK293, and HT1080.
- Stable lentivector-based system
- Great for creating stable reporter cell lines
- Ideal for co-localization studies
- Monitor cellular dynamics and functional studies in real time
- Label cells with GFP for a broad range of studies
Choose the Cyto-Tracer that’s right for you
Promoter (Cat.#) | Cyto-Tracer | Target Location | Peptide Tag |
---|---|---|---|
CMV (CYTO100-PA-1/CYTO100-VA-1) MSCV (CYTO100-PB-1/CYTO100-VB-1) |
pCT-Mem-GFP | Plasma Membrane | Neuromodulin |
CMV (CYTO101-PA-1/CYTO101-VA-1) MSCV (CYTO101-PB-1/CYTO101-VB-1) |
pCT-InnerMem-GFP | Inner Leaflet of Membrane | Farnesylation signal |
CMV (CYTO102-PA-1/CYTO102-VA-1) MSCV (CYTO102-PB-1/CYTO102-VB-1) |
pCT-Mito-GFP | Mitochondria | Cytochrome C oxidase VIII |
CMV (CYTO103-PA-1/CYTO103-VA-1) | pCT-ER-GFP | Endoplasmic Reticulum | ER targeting consensus |
CMV (CYTO104-PA-1/CYTO104-VA-1) | pCT-Golgi-GFP | Golgi Apparatus | Galactosyltrasferase |
CMV (CYTO105-PA-1/CYTO105-VA-1) | pCT-Secretory-GFP | ER-Golgi Vesicles | Secretory consensus peptide |
CMV (CYTO106-PA-1/CYTO106-VA-1) | pCT-Lyso-GFP | Lysosome | Cathepsin B |
CMV (CYTO107-PA-1/CYTO107-VA-1) | pCT-Endo-GFP | Endosome | RhoB |
CMV (CYTO107R-PA-1/CYTO107R-VA-1) | pCT-Endo-RFP | Endosome | RhoB |
CMV (CYTO108-PA-1/CYTO108-VA-1) MSCV (CYTO108-PB-1/CYTO108-VB-1) |
pCT-Pero-GFP | Peroxisome | Peroxisomal consensus signal |
CMV (CYTO120-PA-1/CYTO120-VA-1) | pCT-Autophago-GFP | Autophagosome | LC3 |
CMV (CYTO120R-PA-1/CYTO120R-VA-1) | pCT-Autophago-RFP | Autophagosome | LC3 |
CMV (CYTO109-PA-1/CYTO109-VA-1) | pCT-CD63-GFP | Exosome/Secretory | CD63 Tetraspanin |
CMV (CYTO109R-PA-1/CYTO109R-VA-1) | pCT-CD63-RFP | Exosome/Secretory | CD63 Tetraspanin |
CMV (CYTO122-PA-1/CYTO122-VA-1) | pCT-CD9-GFP | Exosome/Secretory | CD9 Tetraspanin |
CMV (CYTO123-PA-1/CYTO123-VA-1) | pCT-CD9-RFP | Exosome/Secretory | CD9 Tetraspanin |
CMV (CYTO124-PA-1/CYTO124-VA-1) | pCT-CD81-GFP | Exosome/Secretory | CD81 Tetraspanin |
CMV (CYTO125-PA-1/CYTO125-VA-1) | pCT-CD81-RFP | Exosome/Secretory | CD81 Tetraspanin |
CMV (CYTO110-PA-1/CYTO110-VA-1) MSCV (CYTO110-PB-1/CYTO110-VB-1) |
pCT-Actin-GFP | Cytoskeleton | β-actin |
CMV (CYTO111-PA-1/CYTO111-VA-1) MSCV (CYTO111-PB-1/CYTO111-VB-1) |
pCT-Tublin-GFP | Cytoskeleton | α-tublin |
CMV (CYTO111R-PA-1/CYTO111R-VA-1) | pCT-Tublin-RFP | Cytoskeleton | α-tublin |
CMV (CYTO112-PA-1/CYTO112-VA-1) | pCT-MAP4-GFP | Microtubule | Microtubule associated protein 4 (MAP4) |
CMV (CYTO113-PA-1/CYTO113-VA-1) MSCV (CYTO113-PB-1/CYTO113-VB-1) |
pCT-H2B-GFP | Nucleus | H2B |
CMV (CYTO114-PA-1/CYTO114-VA-1) | pCT-Apoptosis-Luc | Caspase 3/7 activation | Cyclic inactive luciferase |
CMV (CYTO115-PA-1/CYTO115-VA-1) | pCT-GFP-BAX | Cytosol to Mitochondria | BAX |
CMV (CYTO116-PA-1/CYTO116-VA-1) | pCT- Catenin-GFP | Cytosol to Nucleus/Cell Membrane | β-Catenin activation |
CMV (CYTO117-PA-1/CYTO117-VA-1) | pCT-CMV-PSD95-GFP | Dendrite Membranes | PSD-95 |
CMV (CYTO118-PA-1/CYTO118-VA-1) MSCV (CYTO118-PB-1/CYTO118-VB-1) |
pCT-Cyto-GFP | Cytosolic Compartment | GFP-Untagged |
CMV (CYTO119-PA-1/CYTO119-VA-1) | pCT-Cyto-RFP | Cytosolic Compartment | RFP-Untagged |
MSCV (CYTO121-PB-1/CYTO121-VB-1) | pCT-Cyto-GFpLuc | GFP and Luciferase Control | GFP and Luciferase (Untagged) |