Cytosol Cyto-Tracer, pCT-Cyto-GFP (CMV)

Overview

Easily study the dynamics of cellular processes with SBI’s Cyto-Tracers™

Leverage SBI’s high-quality lentivector technology to study subcellular localization and dynamics with our line of Cyto-Tracer vectors. Easily create stable reporter cell lines with our validated, pre-built lentivectors—available as transfectable plasmids or ready-to-transduce, pre-packaged virus. The Cytosol Cyto-Tracer, pCT-Cyto-GFP (CMV), expresses an untagged copGFP, which naturally localizes to the cytosol. copGFP is expressed from the CMV promoter for strong expression in common cell types such as HeLa, HEK293, and HT1080.

Stable lentivector-based system
Great for creating stable reporter cell lines
Ideal for co-localization studies
Monitor cellular dynamics and functional studies in real time
Label cells with GFP for a broad range of studies

Choose the Cyto-Tracer that’s right for you

Promoter (Cat.#)	Cyto-Tracer	Target Location	Peptide Tag
CMV (CYTO100-PA-1/CYTO100-VA-1) MSCV (CYTO100-PB-1/CYTO100-VB-1)	pCT-Mem-GFP	Plasma Membrane	Neuromodulin
CMV (CYTO101-PA-1/CYTO101-VA-1) MSCV (CYTO101-PB-1/CYTO101-VB-1)	pCT-InnerMem-GFP	Inner Leaflet of Membrane	Farnesylation signal
CMV (CYTO102-PA-1/CYTO102-VA-1) MSCV (CYTO102-PB-1/CYTO102-VB-1)	pCT-Mito-GFP	Mitochondria	Cytochrome C oxidase VIII
CMV (CYTO103-PA-1/CYTO103-VA-1)	pCT-ER-GFP	Endoplasmic Reticulum	ER targeting consensus
CMV (CYTO104-PA-1/CYTO104-VA-1)	pCT-Golgi-GFP	Golgi Apparatus	Galactosyltrasferase
CMV (CYTO105-PA-1/CYTO105-VA-1)	pCT-Secretory-GFP	ER-Golgi Vesicles	Secretory consensus peptide
CMV (CYTO106-PA-1/CYTO106-VA-1)	pCT-Lyso-GFP	Lysosome	Cathepsin B
CMV (CYTO107-PA-1/CYTO107-VA-1)	pCT-Endo-GFP	Endosome	RhoB
CMV (CYTO107R-PA-1/CYTO107R-VA-1)	pCT-Endo-RFP	Endosome	RhoB
CMV (CYTO108-PA-1/CYTO108-VA-1) MSCV (CYTO108-PB-1/CYTO108-VB-1)	pCT-Pero-GFP	Peroxisome	Peroxisomal consensus signal
CMV (CYTO120-PA-1/CYTO120-VA-1)	pCT-Autophago-GFP	Autophagosome	LC3
CMV (CYTO120R-PA-1/CYTO120R-VA-1)	pCT-Autophago-RFP	Autophagosome	LC3
CMV (CYTO109-PA-1/CYTO109-VA-1)	pCT-CD63-GFP	Exosome/Secretory	CD63 Tetraspanin
CMV (CYTO109R-PA-1/CYTO109R-VA-1)	pCT-CD63-RFP	Exosome/Secretory	CD63 Tetraspanin
CMV (CYTO122-PA-1/CYTO122-VA-1)	pCT-CD9-GFP	Exosome/Secretory	CD9 Tetraspanin
CMV (CYTO123-PA-1/CYTO123-VA-1)	pCT-CD9-RFP	Exosome/Secretory	CD9 Tetraspanin
CMV (CYTO124-PA-1/CYTO124-VA-1)	pCT-CD81-GFP	Exosome/Secretory	CD81 Tetraspanin
CMV (CYTO125-PA-1/CYTO125-VA-1)	pCT-CD81-RFP	Exosome/Secretory	CD81 Tetraspanin
CMV (CYTO110-PA-1/CYTO110-VA-1) MSCV (CYTO110-PB-1/CYTO110-VB-1)	pCT-Actin-GFP	Cytoskeleton	β-actin
CMV (CYTO111-PA-1/CYTO111-VA-1) MSCV (CYTO111-PB-1/CYTO111-VB-1)	pCT-Tublin-GFP	Cytoskeleton	α-tublin
CMV (CYTO111R-PA-1/CYTO111R-VA-1)	pCT-Tublin-RFP	Cytoskeleton	α-tublin
CMV (CYTO112-PA-1/CYTO112-VA-1)	pCT-MAP4-GFP	Microtubule	Microtubule associated protein 4 (MAP4)
CMV (CYTO113-PA-1/CYTO113-VA-1) MSCV (CYTO113-PB-1/CYTO113-VB-1)	pCT-H2B-GFP	Nucleus	H2B
CMV (CYTO114-PA-1/CYTO114-VA-1)	pCT-Apoptosis-Luc	Caspase 3/7 activation	Cyclic inactive luciferase
CMV (CYTO115-PA-1/CYTO115-VA-1)	pCT-GFP-BAX	Cytosol to Mitochondria	BAX
CMV (CYTO116-PA-1/CYTO116-VA-1)	pCT- Catenin-GFP	Cytosol to Nucleus/Cell Membrane	β-Catenin activation
CMV (CYTO117-PA-1/CYTO117-VA-1)	pCT-CMV-PSD95-GFP	Dendrite Membranes	PSD-95
CMV (CYTO118-PA-1/CYTO118-VA-1) MSCV (CYTO118-PB-1/CYTO118-VB-1)	pCT-Cyto-GFP	Cytosolic Compartment	GFP-Untagged
CMV (CYTO119-PA-1/CYTO119-VA-1)	pCT-Cyto-RFP	Cytosolic Compartment	RFP-Untagged
MSCV (CYTO121-PB-1/CYTO121-VB-1)	pCT-Cyto-GFpLuc	GFP and Luciferase Control	GFP and Luciferase (Untagged)

How It Works

Visualizing the cell with Cyto-Tracers

SBI’s Cyto-Tracers fuse a specific protein tag to copGFP or RFP, directing the fluorescent protein to the desired subcellular location.

Supporting Data

See some of our Cyto-Tracers in action

Citations

Kessler, M, et al. (2019) Chronic Chlamydia infection in human organoids increases stemness and promotes age-dependent CpG methylation. Nat Commun. 2019 Mar 18; 10(1):1194. PM ID: 30886143

Gonzalez-King, H, et al. (2017) Hypoxia Inducible Factor-1α Potentiates Jagged 1-Mediated Angiogenesis by Mesenchymal Stem Cell-Derived Exosomes. Stem Cells. 2017 Jul 1; 35(7):1747-1759. PM ID: 28376567

Jawhari, S, et al. (2017) Autophagy and TrkC/NT-3 signaling joined forces boost the hypoxic glioblastoma cell survival. Carcinogenesis. 2017 Jun 1; 38(6):592-603. PM ID: 28402394

Braganza, A, et al. (2017) UBE3B Is a Calmodulin-regulated, Mitochondrion-associated E3 Ubiquitin Ligase. J. Biol. Chem.. 2017 Feb 10; 292(6):2470-2484. PM ID: 28003368

Soares, VYR. (2017) O papel das vesículas extracelulares na fisiopatologia da perda auditiva ocasionada pelo Schwannoma vestibular. Thesis. ;. Link: Thesis

Yoon, C, et al. (2016) Delivery of miR-155 to retinal pigment epithelial cells mediated by Burkitt’s lymphoma exosomes. Tumour Biol.. 2016 Jan 1; 37(1):313-21. PM ID: 26211004

Lo Cicero, A, et al. (2015) Exosomes released by keratinocytes modulate melanocyte pigmentation. Nat Commun. 2015 Jun 24; 6:7506. PM ID: 26103923

Jawhari, S. (2015) Etude des signalisations autophagique et neurotrophique dans des lignées de glioblastome humain activées lors de l’hypoxie. Thesis. ;. Link: Thesis

VARLET, P, PU-PH, P & DJAVAHERI-MERGNY, M. (2015) SOHA JAWHARI. Thesis. ;. Link: Thesis

Liang, Y. (2015) Exosome/Microvesicle Cargo Contents Recruitment in a Glycosylation-dependent Pathway. Thesis. ;. Link: Thesis

Fang, Q, et al. (2014) HSP90 regulates DNA repair via the interaction between XRCC1 and DNA polymerase β. Nat Commun. 2014 Nov 26; 5:5513. PM ID: 25423885

Liang, Y, et al. (2014) Complex N-linked glycans serve as a determinant for exosome/microvesicle cargo recruitment. J. Biol. Chem.. 2014 Nov 21; 289(47):32526-37. PM ID: 25261472

Sato, Y, et al. (2014) MTCL1 crosslinks and stabilizes non-centrosomal microtubules on the Golgi membrane. Nat Commun. 2014 Nov 4; 5:5266. PM ID: 25366663

Cytosol Cyto-Tracer™, pCT-Cyto-GFP (CMV)

Study cellular processes and physiology with this Cyto-Tracer that expresses un-tagged copGFP from the CMV promoter - untagged copGFP localizes in the cytosol

Overview

How It Works

Supporting Data

Product Documentation

Citations

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Study cellular processes and physiology with this Cyto-Tracer that expresses un-tagged copGFP from the CMV promoter - untagged copGFP localizes in the cytosol

Overview

How It Works

Supporting Data

Product Documentation

Citations

Resources

Related Products

PRODUCTS

SERVICES

RESOURCES

Blog

Support