Monitor the balance of T cell dynamics in autoimmunity, inflammation, and cancer
Simplify your studies of T cell dynamics with SBI’s ready-to-use, pre-built Treg and Th17 vectors and cell lines. With the Mouse RORγt Promoter GreenFire Lentivector you can combine SBI’s well-regarded and high quality lentivector technology with a RORγt promoter-reporter architecture that enables easy and quantitative monitoring of RORγt expression. The RORγt promoter drives coordinated expression of GFP and luciferase reporters.
The promoter sequences and design are based upon Dang, et al.1, Lazarevic, et al.2, and Ruan, et al3.
- Dang, EV, et al. Nuclear factor-κB in immunity and inflammation: the Treg and Th17 connection. Adv Exp Med Biol. 2012; 946:207-21. PMID: 21948370.
- Lazarevic, V. et al. T-bet represses T(H)17 differentiation by preventing Runx1-mediated activation of the gene encoding RORγt. Nat Immunol. 2011 Jan; 12(1):96-104. PMCID: PMC3077962.
- Ruan, Q, et al. The Th17 immune response is controlled by the Rel-RORγ-RORγ T transcriptional axis. J Exp Med. 2011 Oct 24; 208(11):2321-33. PMCID: PMC3201209.