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96-well ExoELISA Plate

Pack of ten 96-well ExoELISA plates optimized for use with any of SBI’s ExoELISA assays.

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96-well ExoELISA plate (12×8-well strips, pack of 10 plates)
10 Plates
$ 153


Additional plates optimized for our ExoELISA Kits

For researchers who just need additional ExoELISA plates, we offer 96-well plates as a pack of ten. Each 96-well plate comes as a 12 strips of 8 wells each to maximize your flexibility—unused strips can be stored at room temperature for later use.

Choose the exosome quantitation method that’s best for your studies

EXOCET FluoroCet
Use For fast and sensitive antibody-based quantitation of exosomes For sensitive quantitation of exosomes when time and input sample are not limiting For fast quantitation of extracellular vesicles with moderate sample input requirements For the most sensitive quantitation of extracellular vesicles with very low sample input requirements
Detection method Antibody Antibody Enzymatic Enzymatic
Quantitation chemistry Enzymatic (HRP) Enzymatic (HRP) Colorimetric Fluorescent
Total protocol time 4 hours (no overnight incubation) 24 hours 20 min 60 min
Input sample amount (protein equivalent) 1 – 200 µg >500 µg 50 µg <1 µg
Learn More ExoELISA-ULTRA CD63


EXOCET FluoroCet

How It Works

Our ExoELISA Kits have all the reagents you need to run the ELISA—just add lysed exosome particles. The kits are compatible with exosomes isolated using most methods, including ExoQuick®, ExoQuick-TC®, or ultracentrifugation.

The lysed exosome particles (and, thus, exosomal proteins) are directly immobilized onto the wells of the microtiter plate, and after binding, a blocking agent is added to prevent non-specific binding of the primary detection antibody (anti-CD9, -CD63, or -CD81). Following addition of the primary antibody, a secondary antibody (goat anti-rabbit) linked to horseradish peroxidase (HRP) is also added to amplify the signal and increase assay sensitivity.

The amount of the exosome marker (CD9, CD63, or CD81) is measured via activity of the bound HRP-secondary antibody using a colorimetric assay with extra-sensitive TMB as the substrate. The accumulation of colored product is proportional to the amount of marker present in each well, and is measured using a microtiter plate reader at 450 nm absorbance.

Each ExoELISA Kit includes a set of standards calibrated to a known amount of exosome particles as determined by NanoSight analysis. These standards can be used to generate a calibration curve enabling quantitation of exosomes carrying the marker of interest from the ExoELISA data.


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