Exo-APC Exosome FACS Stain
An APC-based stain to visualize exosomes immobilized to Exo-Flow beads for FACS and other imaging applications
Visualize bead-immobilized exosomes with our proprietary, reversible APC-based stain
Optimized for our Exo-Flow Capture Kits and available only from SBI, Exo-APC is a proprietary reversible fluorescent stain for visualizing exosomes. Exo-APC is comprised of the commonly used fluorophore allophycocyanin (APC) conjugated to a protein that recognizes post-translational modifications on exosomal surface proteins, enabling general staining of exosomes.
However, the feature that really makes Exo-APC stand out as an ideal reagent for FACS-based isolation of exosomes is its reversibility—after staining with Exo-APC and flow-sorting by APC-positive gating (Exo-APC’s excitation and emission wavelengths are 650 nm and 660 nm, respectively), Exo-APC can be removed from exosomes through the simple addition of Exosome Elution Buffer, which simultaneously elutes exosomes from the Exo-Flow magnetic beads and removes bound Exo-APC.
Note that we also offer reversible Exo-FITC (Cat.# Exo-FLOW800A-1) as an alternative stain to Exo-APC.
Use Exo-APC with any of our Exo-Flow Capture Kits:
|EXOFLOW100A-1||CD9 Exo-Flow Capture Kit|
|EXOFLOW150A-1||Tetraspanin Exo-Flow Combo Capture Kit|
|EXOFLOW200A-1||CD31 Exo-Flow Capture Kit|
|EXOFLOW210A-1||CD44 Exo-Flow Capture Kit|
|EXOFLOW300A-1||CD63 Exo-Flow Capture Kit|
|EXOFLOW400A-1||CD81 Exo-Flow Capture Kit|
|EXOFLOW500A-1||Rab5b Exo-Flow Capture Kit|
|EXOFLOW600A-1||HLA-G Exo-Flow Capture Kit|
|EXOFLOW610A-1||CD14 Exo-Flow Capture Kit|
|EXOFLOW620A-1||CD68 Exo-Flow Capture Kit|
|EXOFLOW630A-1||EpCAM Exo-Flow Capture Kit|
|EXOFLOW660A-1||CD73 Exo-Flow Capture Kit|
How It Works
Easily purify exosomes using FACS with our Exo-Flow Capture Kits
At a glance
Simply (1) couple biotinylated antibody to the magnetic streptavidin beads, (2) use the antibody-coupled magnetic beads to capture exosomes that have been isolated using either ExoQuick® or ultracentrifugation, (3) wash away unbound exosomes, and then (4) stain with reversible Exo-APC (excitation and emission wavelengths of 650 nm and 660 nm, respectively).
Your sample is now ready for FACS analysis.
To use the purified exosomes after FACS, add the included Exosome Elution Buffer to simultaneously remove the Exo-APC stain and elute intact exosomes from the beads.
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