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mRNAExpress™ hMyoD Transcript

Save time and deliver human hMyoD mRNA to cells for differentiation or other studies using this ready-to-transfect, pre-made mRNAExpress transcript

  • An anti-reverse cap analog (ARCA) to increase translation efficiency
  • Modified nucleotides (5-Methylcytidine-5′-Triphosphate and Pseudouridine-5′-Triphosphate) and a poly-A tail to enhance stability and reduce host cell immune response
  • Removal of the 5′ triphosphates at the end of the RNA to further reduce innate immune responses in mammalian cells
Catalog Number
Description
Size
Price
Quantity
Add to Cart
MR900A-1
mRNAExpress hMyoD transcript
10 µg
$ 213
MR900A-2
mRNAExpress hMyoD transcript
20 µg
$ 306

Overview

Save time by delivering pre-made mRNAs

Save time when you want instant expression after transfection and use one of SBI’s pre-made, ready-to-transfect mRNAs. Like all of our pre-made mRNAs, the mRNAExpress™ hMyoD Transcript contains a number of different features to enhance performance:

  • An anti-reverse cap analog (ARCA) to increase translation efficiency
  • Modified nucleotides (5-Methylcytidine-5′-Triphosphate and Pseudouridine-5′-Triphosphate) and a poly-A tail to enhance stability and reduce host cell immune response
  • Removal of the 5′ triphosphates at the end of the RNA to further reduce innate immune responses in mammalian cells

Generate induced pluripotent stem cells (iPSCs) with mRNAExpress transcripts:

Cat.# mRNAExpress Transcript
MR100A-1 mRNAExpress hOct4 transcript
MR200A-1 mRNAExpress hSox2 transcript
MR300A-1 mRNAExpress hKlf4 transcript
MR400A-1 mRNAExpress hcMyc transcript
MR500A-1 mRNAExpress hLin28 transcript
MR600A-1 mRNAExpress hNanog transcript
MR700A-1 mRNAExpress GFP transcript (positive control)

Supporting Data

The mRNAExpress hMyoD transcript drives transdifferentiation

Figure 1. The mRNAExpress hMyoD transcript drives transdifferentiation into myocytes. Human foreskin fibroblasts were transfected with 1 µg of the mRNAExpress hMyoD Transcript per 24-well plate three times on three consecutive days (transfection was enhanced with RNAfection Transfection Reagent (Cat.# MR750A-1)). After an additional three days of transdifferentiation, the cells were immunostained for Desmin, a myogenic marker, to visualize myotube formation, and nuclei imaged with DAPI. The mRNAExpress hMyoD Transcript successfully transdifferentiated fibroblasts into myocytes.

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