mRNAExpress™ hMyoD Transcript
Save time and deliver human hMyoD mRNA to cells for differentiation or other studies using this ready-to-transfect, pre-made mRNAExpress transcript
- An anti-reverse cap analog (ARCA) to increase translation efficiency
- Modified nucleotides (5-Methylcytidine-5′-Triphosphate and Pseudouridine-5′-Triphosphate) and a poly-A tail to enhance stability and reduce host cell immune response
- Removal of the 5′ triphosphates at the end of the RNA to further reduce innate immune responses in mammalian cells
Overview
Save time by delivering pre-made mRNAs
Save time when you want instant expression after transfection and use one of SBI’s pre-made, ready-to-transfect mRNAs. Like all of our pre-made mRNAs, the mRNAExpress™ hMyoD Transcript contains a number of different features to enhance performance:
- An anti-reverse cap analog (ARCA) to increase translation efficiency
- Modified nucleotides (5-Methylcytidine-5′-Triphosphate and Pseudouridine-5′-Triphosphate) and a poly-A tail to enhance stability and reduce host cell immune response
- Removal of the 5′ triphosphates at the end of the RNA to further reduce innate immune responses in mammalian cells
Generate induced pluripotent stem cells (iPSCs) with mRNAExpress transcripts:
| Cat.# | mRNAExpress Transcript |
|---|---|
| MR100A-1 | mRNAExpress hOct4 transcript |
| MR200A-1 | mRNAExpress hSox2 transcript |
| MR300A-1 | mRNAExpress hKlf4 transcript |
| MR400A-1 | mRNAExpress hcMyc transcript |
| MR500A-1 | mRNAExpress hLin28 transcript |
| MR600A-1 | mRNAExpress hNanog transcript |
| MR700A-1 | mRNAExpress GFP transcript (positive control) |
Supporting Data
The mRNAExpress hMyoD transcript drives transdifferentiation
Figure 1. The mRNAExpress hMyoD transcript drives transdifferentiation into myocytes. Human foreskin fibroblasts were transfected with 1 µg of the mRNAExpress hMyoD Transcript per 24-well plate three times on three consecutive days (transfection was enhanced with RNAfection Transfection Reagent (Cat.# MR750A-1)). After an additional three days of transdifferentiation, the cells were immunostained for Desmin, a myogenic marker, to visualize myotube formation, and nuclei imaged with DAPI. The mRNAExpress hMyoD Transcript successfully transdifferentiated fibroblasts into myocytes.
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