pCDH-CMV-MCS-EF1α-Puro Cloning and Expression Lentivector

With the strong CMV promoter upstream of an MCS and EF1α driving the puromycin resistance marker, pCDH-CMV-MCS-EF1α-Puro is a great, all-purpose lentivector
  • Multiple promoter options
  • Multiple marker options—Puro, Neo, Hygro, GFP, or RFP
  • Coordinated co-expression via T2A or IRES
  • Single promoter, dual promoter, and bidirectional promoter formats
  • High expression in most hematopoietic, embryonic, and stem cells via the MSCV CpG-deficient promoter

Products

Catalog Number Description Size Price Quantity Add to Cart
CD510B-1 pCDH-CMV-MCS-EF1α-Puro cDNA Dual Promoter Cloning and Expression Lentivector 10 µg $593
- +

Overview

Overview

Get robust, stable expression of your gene-of-interest in dividing and quiescent cells

Well-regarded in the industry for high, reliable gene expression, SBI’s lentiviral vectors come in a variety of formats that support a wide range of applications. The pCDH-CMV-MCS-EF1α-Puro Cloning and Expression Lentivector drives expression of your gene-of-interest from the strong CMV promoter, and expression of the puromycin resistance marker from the EF1α promoter.

pCDH-CMV-MCS-EF1α-Puro Cloning and Expression Lentivector With over thirty HIV-based lentivector options for gene expression, SBI’s family of pCDH Cloning and Expression Lentivectors provide the right lentivector for almost any gene expression project:
  • Multiple promoter options
  • Multiple marker options—Puro, Neo, Hygro, GFP, or RFP
  • Coordinated co-expression via T2A or IRES
  • Single promoter, dual promoter, and bidirectional promoter formats
  • High expression in most hematopoietic, embryonic, and stem cells via the MSCV CpG-deficient promoter
Not sure which promoter is right for you? This table should help:
PromoterExpression LevelApplication
CMVHighCommonly used in most cell lines (HeLa, HEK293, HT1080, etc.)
MSCVHighHematopoietic and stem cells
EF1αMediumMost cell types including primary cells and stem cells
PGKMediumMost cell types including primary cells and stem cells
UbCLowMost cell types including primary cells and stem cells

How It Works

Supporting Data

FAQs

Resources

Citations

  • Ge, LP, et al. (2024) ZNF689 deficiency promotes intratumor heterogeneity and immunotherapy resistance in triple-negative breast cancer. Cell research. 2024; 34(1):58-75. PM ID: 38168642
  • Zhai, X, et al. (2024) LDLR is used as a cell entry receptor by multiple alphaviruses. Nature communications. 2024; 15(1):622. PM ID: 38245515
  • Sharma, P, et al. (2024) Disordered-to-ordered transitions in assembly factors allow the complex II catalytic subunit to switch binding partners. Nature communications. 2024; 15(1):473. PM ID: 38212624
  • Zhu, Q, et al. (2024) Stabilization of Pin1 by USP34 promotes Ubc9 isomerization and protein sumoylation in glioma stem cells. Nature communications. 2024; 15(1):40. PM ID: 38167292
  • Chen, SY, et al. (2024) Spermatid perinuclear RNA-binding protein promotes UBR5-mediated proteolysis of Dicer to accelerate triple-negative breast cancer progression. Cancer letters. 2024;:216672. PM ID: 38280476
  • Shi, T, et al. (2024) Bivalent activity of super-enhancer RNA LINC02454 controls 3D chromatin structure and regulates glioma sensitivity to temozolomide. Cell death & disease. 2024; 15(1):6. PM ID: 38177123
  • Rivera, M, et al. (2024) Malignant A-to-I RNA editing by ADAR1 drives T cell acute lymphoblastic leukemia relapse via attenuating dsRNA sensing. Cell reports. 2024; 43(2):113704. PM ID: 38265938
  • He, J, et al. (2024) Exosome-specific loading Sox10 for the treatment of Cuprizone-induced demyelinating model. Biomedicine & pharmacotherapy = Biomedecine & pharmacotherapie. 2024; 171:116128. PM ID: 38218078
  • Xiao, G, et al. (2024) Fbxw7 suppresses carcinogenesis and stemness in triple-negative breast cancer through CHD4 degradation and Wnt/β-catenin pathway inhibition. Journal of translational medicine. 2024; 22(1):99. PM ID: 38268032
  • Wang, Q, et al. (2024) The pleiotropic enhancer enh9 promotes cell proliferation and migration in non-small cell lung cancer via ERMP1 and PD-L1. Biochimica et biophysica acta. Molecular basis of disease. 2024; 1870(3):167015. PM ID: 38182069
  • Samer, C, et al. (2024) Multi-targeted loss of the antigen presentation molecule MR1 during HSV-1 and HSV-2 infection. iScience. 2024; 27(2):108801. PM ID: 38303725
  • Shen, Z, et al. (2024) Intrahepatic homeobox protein MSX-1 is a novel host restriction factor of hepatitis B virus. Journal of virology. 2024;:e0134523. PM ID: 38226815
  • Wu, H, et al. (2024) METTL14/miR-29c-3p axis drives aerobic glycolysis to promote triple-negative breast cancer progression though TRIM9-mediated PKM2 ubiquitination. Journal of cellular and molecular medicine. 2024;. PM ID: 38263865
  • Gong, Y, et al. (2024) Transcriptome sequencing analysis reveals miR-30c-5p promotes ferroptosis in cervical cancer and inhibits growth and metastasis of cervical cancer xenografts by targeting the METTL3/KRAS axis. Cellular signalling. 2024;:111068. PM ID: 38286198
  • Hong, J, et al. (2024) All-in-one IQ toggle switches with high versatilities for fine tuning of transgene expression in mammalian cells and tissues. Molecular Therapy - Methods & Clinical Development. 2024;:101202. Link: Molecular Therapy - Methods & Clinical Development
  • Li, J, et al. (2024) ECM1-associated miR-1260b promotes osteogenic differentiation by targeting GDI1. Acta histochemica. 2024; 126(1):152133. PM ID: 38266317
  • Yang, W, et al. (2024) Protein SUMOylation promotes cAMP-independent EPAC1 activation. bioRxiv : the preprint server for biology. 2024;. PM ID: 38260470
  • Wu, Z, et al. (2024) Targeting CaMKII-δ/DHCR24 axis is a novel strategy against acute myeloid leukemia. Research Square. 2024;. Link: Research Square
  • Bataclan, M, et al. (2024) Crosstalk between the RNA-binding proteins Regnase-1 and -3 shapes mast cell survival and cytokine expression. bioRxiv. 2024;. Link: bioRxiv
  • Giblin, A, et al. (2024) Neuronal polyunsaturated fatty acids are protective in FTD/ALS. bioRxiv. 2024;. Link: bioRxiv

Products

Catalog Number Description Size Price Quantity Add to Cart
CD510B-1 pCDH-CMV-MCS-EF1α-Puro cDNA Dual Promoter Cloning and Expression Lentivector 10 µg $593
- +

Overview

Overview

Get robust, stable expression of your gene-of-interest in dividing and quiescent cells

Well-regarded in the industry for high, reliable gene expression, SBI’s lentiviral vectors come in a variety of formats that support a wide range of applications. The pCDH-CMV-MCS-EF1α-Puro Cloning and Expression Lentivector drives expression of your gene-of-interest from the strong CMV promoter, and expression of the puromycin resistance marker from the EF1α promoter.

pCDH-CMV-MCS-EF1α-Puro Cloning and Expression Lentivector With over thirty HIV-based lentivector options for gene expression, SBI’s family of pCDH Cloning and Expression Lentivectors provide the right lentivector for almost any gene expression project:
  • Multiple promoter options
  • Multiple marker options—Puro, Neo, Hygro, GFP, or RFP
  • Coordinated co-expression via T2A or IRES
  • Single promoter, dual promoter, and bidirectional promoter formats
  • High expression in most hematopoietic, embryonic, and stem cells via the MSCV CpG-deficient promoter
Not sure which promoter is right for you? This table should help:
PromoterExpression LevelApplication
CMVHighCommonly used in most cell lines (HeLa, HEK293, HT1080, etc.)
MSCVHighHematopoietic and stem cells
EF1αMediumMost cell types including primary cells and stem cells
PGKMediumMost cell types including primary cells and stem cells
UbCLowMost cell types including primary cells and stem cells

How It Works

Supporting Data

FAQs

Citations

  • Ge, LP, et al. (2024) ZNF689 deficiency promotes intratumor heterogeneity and immunotherapy resistance in triple-negative breast cancer. Cell research. 2024; 34(1):58-75. PM ID: 38168642
  • Zhai, X, et al. (2024) LDLR is used as a cell entry receptor by multiple alphaviruses. Nature communications. 2024; 15(1):622. PM ID: 38245515
  • Sharma, P, et al. (2024) Disordered-to-ordered transitions in assembly factors allow the complex II catalytic subunit to switch binding partners. Nature communications. 2024; 15(1):473. PM ID: 38212624
  • Zhu, Q, et al. (2024) Stabilization of Pin1 by USP34 promotes Ubc9 isomerization and protein sumoylation in glioma stem cells. Nature communications. 2024; 15(1):40. PM ID: 38167292
  • Chen, SY, et al. (2024) Spermatid perinuclear RNA-binding protein promotes UBR5-mediated proteolysis of Dicer to accelerate triple-negative breast cancer progression. Cancer letters. 2024;:216672. PM ID: 38280476
  • Shi, T, et al. (2024) Bivalent activity of super-enhancer RNA LINC02454 controls 3D chromatin structure and regulates glioma sensitivity to temozolomide. Cell death & disease. 2024; 15(1):6. PM ID: 38177123
  • Rivera, M, et al. (2024) Malignant A-to-I RNA editing by ADAR1 drives T cell acute lymphoblastic leukemia relapse via attenuating dsRNA sensing. Cell reports. 2024; 43(2):113704. PM ID: 38265938
  • He, J, et al. (2024) Exosome-specific loading Sox10 for the treatment of Cuprizone-induced demyelinating model. Biomedicine & pharmacotherapy = Biomedecine & pharmacotherapie. 2024; 171:116128. PM ID: 38218078
  • Xiao, G, et al. (2024) Fbxw7 suppresses carcinogenesis and stemness in triple-negative breast cancer through CHD4 degradation and Wnt/β-catenin pathway inhibition. Journal of translational medicine. 2024; 22(1):99. PM ID: 38268032
  • Wang, Q, et al. (2024) The pleiotropic enhancer enh9 promotes cell proliferation and migration in non-small cell lung cancer via ERMP1 and PD-L1. Biochimica et biophysica acta. Molecular basis of disease. 2024; 1870(3):167015. PM ID: 38182069
  • Samer, C, et al. (2024) Multi-targeted loss of the antigen presentation molecule MR1 during HSV-1 and HSV-2 infection. iScience. 2024; 27(2):108801. PM ID: 38303725
  • Shen, Z, et al. (2024) Intrahepatic homeobox protein MSX-1 is a novel host restriction factor of hepatitis B virus. Journal of virology. 2024;:e0134523. PM ID: 38226815
  • Wu, H, et al. (2024) METTL14/miR-29c-3p axis drives aerobic glycolysis to promote triple-negative breast cancer progression though TRIM9-mediated PKM2 ubiquitination. Journal of cellular and molecular medicine. 2024;. PM ID: 38263865
  • Gong, Y, et al. (2024) Transcriptome sequencing analysis reveals miR-30c-5p promotes ferroptosis in cervical cancer and inhibits growth and metastasis of cervical cancer xenografts by targeting the METTL3/KRAS axis. Cellular signalling. 2024;:111068. PM ID: 38286198
  • Hong, J, et al. (2024) All-in-one IQ toggle switches with high versatilities for fine tuning of transgene expression in mammalian cells and tissues. Molecular Therapy - Methods & Clinical Development. 2024;:101202. Link: Molecular Therapy - Methods & Clinical Development
  • Li, J, et al. (2024) ECM1-associated miR-1260b promotes osteogenic differentiation by targeting GDI1. Acta histochemica. 2024; 126(1):152133. PM ID: 38266317
  • Yang, W, et al. (2024) Protein SUMOylation promotes cAMP-independent EPAC1 activation. bioRxiv : the preprint server for biology. 2024;. PM ID: 38260470
  • Wu, Z, et al. (2024) Targeting CaMKII-δ/DHCR24 axis is a novel strategy against acute myeloid leukemia. Research Square. 2024;. Link: Research Square
  • Bataclan, M, et al. (2024) Crosstalk between the RNA-binding proteins Regnase-1 and -3 shapes mast cell survival and cytokine expression. bioRxiv. 2024;. Link: bioRxiv
  • Giblin, A, et al. (2024) Neuronal polyunsaturated fatty acids are protective in FTD/ALS. bioRxiv. 2024;. Link: bioRxiv