PinPoint-FC 293T Platform Cell Line for Targeted Gene Insertion (PinPoint attP already placed)
Jump start your PinPoint cell line creation with this 293T cell line that already has a single PinPoint attP site inserted into the genome
Making the two-step PinPoint process a single, easy step
With the PinPoint-FC 293T Platform Cell Line for Targeted Gene Insertion you can skip the first step of the PinPoint Targeted Integration workflow because SBI’s already done it for you. This cell line comes with a PinPoint attP site already placed and validated to be present in single copy, so you can simply co-transfect with the PinPoint Integrase Expression Vector and an appropriate PinPoint Donor Vector.
With the PinPoint-FC 293T Platform Cell Line for Targeted Gene Insertion, generating isogenic cell lines becomes a straightforward, one-step process that can be easily implemented as a high-throughput assay.
How It Works
Move a step ahead with the PinPoint-FC293T Platform Cell Line
Engineering cells with the PinPoint-FC 293T Platform Cell Line for Targeted Gene Insertion simplifies a two-step process into a single step. You can skip the placement of the PinPoint attP site and associated validation to verify copy number, and move straight to the insertion of your expression cassette with the PinPoint Donor Vector and PinPoint Integrase.
The PinPoint Integrase mediates a recombination event between the PinPoint attB site (located on the PinPoint Vector) and the PinPoint attP site. Because the PinPoint Integrase only recognizes the pre-placed PinPoint attP site, you get very stringent control of integration location.