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pPACK-SPIKE™ P1 Variant Spike Protein Lentivector Packaging Mix

Study the spike protein from SARS-CoV-2 Variant P1, a CDC variant of concern, with this packaging mix for lentivirus pseudotyped with the P1 spike protein

  • Based on SBI’s popular and highly cited pPACKH1 Packaging System
  • Uses codon-optimized SARS-CoV-2 “S” protein from variant P1 in place of VSV-G envelope protein
  • Spike protein mutations are L18F, T20N, P26S, D138Y, R190S, K417T, E484K, N501Y, D614G, H655Y, T1027I, V1176F
  • Ideal for vaccine and antiviral efficacy studies under BSL2 conditions
  • Package any 3rd-generation lentivector reporter of your choice, including SBI’s popular LentiLabeler reporters
Catalog Number
Description
Size
Price
Quantity
Add to Cart
CVD19-630A-1
pPACK-SPIKE P1, SARS-CoV-2 “S” Pseudotype – P1 Variant – Lentivector Packaging Mix
10 Reactions
$ 650
CVD19-635A-1
pPACK-SPIKE P1, SARS-CoV-2 “S” Pseudotype – P1 Variant – Lentivector Packaging Mix
25 Reactions
$ 1295
CVD19-639A-KIT
pPACK-SPIKE P1 Combo Kit, includes Cat# CVD19-630A-1, plus PureFection Transfection Reagent (Cat# LV750A-1) and PEG-it Virus Concentration solution (Cat# LV810A-1)
1 Kit
$ 1095

Overview

Safely study the spike protein from emerging SARS-CoV-2 variants 

Classified as a variant of concern by the Centers for Disease Control (CDC) in the United States, the SARS-CoV-2 P1 Variant has been a major driver of disease in Brazil and shows increased transmissibility [1] and increased resistance to neutralization [2]. With the pPACK-SPIKE™ P1 Variant Spike Protein Lentivector Packaging Mix, you can safely characterize the SARS-CoV-2 S1 spike protein from the emerging lineage P1, which contains the following mutations:

  • L18F
  • T20N
  • P26S
  • D138Y
  • R190S
  • K417T
  • E484K
  • N501Y
  • D614G
  • H655Y
  • T1027I
  • V1176F

Designed to efficiently package most third-generation lentivectors, the pPACK-SPIKE P1 Variant Spike Protein Lentivector Packaging Mix speeds and simplifies preparation of lentiviral particles pseudotyped with the SARS-CoV-2 L18F, T20N, P26S, D138Y, R190S, K417T, E484K, N501Y, D614G, H655Y, T1027I, V1176F spike glycoprotein, making it an ideal reagent for vaccine and antiviral drug discovery projects.

Based on SBI’s highly cited pPACKH1 packaging system but with a truncated SARS-CoV-2 L18F, T20N, P26S, D138Y, R190S, K417T, E484K, N501Y, D614G, H655Y, T1027I, V1176F Spike protein [1] replacing the standard VSV-G envelope protein, the pPACK-SPIKE P1 Variant Spike Protein Packaging Mix consists of three plasmids that produce all of the structural and  replication proteins needed to transcribe and package an RNA copy of an expression lentivector into recombinant, L18F, T20N, P26S, D138Y, R190S, K417T, E484K, N501Y, D614G, H655Y, T1027I, V1176F “Spike” pseudotyped lentiviral particles. As a result, you can conduct a range of SARS-CoV-2 studies under BSL-2 conditions, including neutralization assays, studies of virus interactions with host surface proteins, and the development of vaccines and therapeutics.

For added convenience we also offer pPACK-BALD™, an envelope protein-free lentivector packaging mix that can be used as a negative control for any pPACK-SPIKE study, or for creating lentivirus particles pseudotyped with the envelope protein of your choice.

  • Based on SBI’s popular and highly cited pPACKH1 Packaging System
  • Uses codon-optimized SARS-CoV-2 “S” protein from variant P1 in place of VSV-G envelope protein
  • Spike protein mutations are L18F, T20N, P26S, D138Y, R190S, K417T, E484K, N501Y, D614G, H655Y, T1027I, V1176F
  • Ideal for vaccine and antiviral efficacy studies under BSL2 conditions
  • Package any 3rd-generation lentivector reporter of your choice, including SBI’s popular LentiLabeler reporters
  • Compare with our full range of pPACK-SPIKE S protein pseudotyped lentiviruses to better understand emerging variants
  • Use with pPACK-BALD™, an envelope protein-free lentivector packaging system that is an ideal negative control

pPACK-SPIKE P1 Variant Spike Protein Lentivector Packaging Mix is available as stand-alone packaging mixes with sufficient plasmids for 10 reactions (standard size) or 25 reactions (XL), as well as in a convenient kit format that includes PureFection™ Transfection Reagent and PEG-it Virus Precipitation Solution.

See all of the available pPACK-SPIKE S protein variants:

Catalog NumberProduct DescriptionSpike protein mutation
CVD19-500A-1pPACK-SPIKE SARS-CoV-2 “S” Pseudotype Lentivector Packaging MixOriginal Wuhan-Hu-1 strain
CVD19-530A-1pPACK-SPIKE D614G, SARS-CoV-2 “S” Pseudotype – D614G Mutant – Lentivector Packaging MixD614G
CVD19-560A-1pPACK-SPIKE N501Y, SARS-CoV-2 "S" Pseudotype - N501Y Mutant - Lentivector Packaging MixN501Y
CVD19-580A-1pPACK-SPIKE B.1.351 RBD Mutations Lentivector Packaging MixK417N, E484K, & N501Y
CVD19-600A-1pPACK-SPIKE B.1.351 S1 Mutations (RBD+D614G) Lentivector Packaging MixK417N, E484K, N501Y, & D614G
CVD19-590A-1pPACK-SPIKE B.1.1.7, SARS-CoV-2 "S" Pseudotype - B.1.1.7 (UK) Variant - Lentivector Packaging MixΔH69/V70, ΔY144, N501Y, A570D, D614G, P681H, T716I, S982A, D1118H
CVD19-610A-1pPACK-SPIKE B.1.429, SARS-CoV-2 "S" Pseudotype - B.1.429 (CAL.20C) Variant - Lentivector Packaging MixS13I, W152C, L452R, & D614G
CVD19-620A-1pPACK-SPIKE B.1.525, SARS-CoV-2 "S" Pseudotype - B.1.525 Variant - Lentivector Packaging MixΔH69/V70, Q52R, E484K, D614G, Q677H, & F888L
CVD19-630A-1pPACK-SPIKE P1, SARS-CoV-2 "S" Pseudotype - P1 Variant - Lentivector Packaging MixL18F, T20N, P26S, D138Y, R190S, K417T, E484K, N501Y, D614G, H655Y, T1027I, V1176F
LV550A-1pPACK-BALD™ (Negative Control Packaging Mix)An envelope protein-free negative control for pPACK-SPIKE

References

  1. Coutinho RM, et al. Model-based estimation of transmissibility and reinfection of SARS-CoV-2 P.1 variant. medRxiv. 2021.03.03.21252706; doi: https://doi.org/10.1101/2021.03.03.21252706.
  2. Wang P, et al. Increased Resistance of SARS-CoV-2 Variant P.1 to Antibody Neutralization. bioRxiv. 2021.03.01.433466; doi: https://doi.org/10.1101/2021.03.01.433466.
  3. Ou X, et al. Characterization of spike glycoprotein of SARS-CoV-2 on virus entry and its immune cross-reactivity with SARS-CoV. Nat. commun. 2020 Mar 27;11(1):1620. doi: 10.1038/s41467-020-15562-9. PMCID: PMC7100515.

How It Works

pPACK-SPIKE is easy to use—simply co-transfect the pPACK-SPIKE Plasmids and your lentivector construct into 293TN producer cells or other lentiviral production cells, isolate pseudoviral particles with an optional concentration step, and conduct your studies.

Supporting Data

To see data generated using the pPACK-SPIKE with the S protein from the original Wuhan-Hu-1 strain, visit the pPACK-SPIKE  page.

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