RNA-Quant™ cDNA Synthesis Kit
Easily profile any RNA—small or long, non-coding or coding—via real-time qPCR with this comprehensive cDNA synthesis kit
Efficiently prepare any RNA for qPCR profiling
With a single cDNA synthesis reaction, you can efficiently prepare all of the RNAs in your sample for real-time qPCR using the RNA-Quant™ cDNA Synthesis Kit. Unlike other cDNA synthesis kits which can only prepare one or two classes of RNAs for qPCR, the RNA-Quant Kit enables qPCR measurement of many classes of RNA from a single cDNA synthesis reaction. The kit includes three endogenous RNA reference qPCR assays as for human and mouse samples—miR-16, Y RNA lncRNA, and GAPDH mRNA.
An all-in-one cDNA synthesis kit
- Tail and tag all RNAs for cDNA synthesis
- Obtain full RNA coverage for any purpose
- Profile any RNA—mRNA, miRNA, lncRNA, sn/snoRNA, rRNA
How It Works
The RNA-Quant cDNA Synthesis Kit prepares any RNA for qPCR
The poly-A tailing and tagging method in the RNA-Quant System generates cDNA that is highly unique and, when used with the 3’ Universal Reverse Primer, does not produce any signal in the absence of reverse transcriptase or lead to the detection of any residual genomic DNA. The result is zero background for RNA profiling with complete confidence.
- Tag all small RNAs with a poly-A tail
- Anneal an oligo-dT adaptor
- Reverse transcribe to create first-strand cDNA
Your final product will be a cDNA pool of anchor-tailed miRNAs that are ready for qPCR.