Home | Products | Uncategorized | sample product table

sample product table

Regulate gene expression or study miRNA function with lentivector-delivered precursor miRs that get efficiently and accurately processed into mature miRs.

There are 611 individual products in this category.

Want to speak to a specialist? Contact Us or 1-888-266-5066

Products

Available Human pre-miRNA Expression Lentivectors (Lenti-miRs)

Overview

Streamline your studies and maximize your options for miR expression

Whether you’re using miRNAs to study cellular processes or develop the next generation of therapeutics, SBI’s comprehensive collection of Lenti-miRsTM—human precursor miRNA lentivectors—are a superior alternative to synthetic RNAs. Like synthetic miRs, SBI’s Lenti-miRs can be transfected into target cells for transient miR expression. But unlike synthetic miRs, they can also be transduced into a variety of target cells—including primary cells, stem cells, and other hard-to-transfect cell lines—to create stable miR-producing cell lines, maximizing your options for miR expression.

Why else choose SBI’s precursor miRNA lentivectors?

In addition, SBI’s Lenti-miRs are designed for efficient expression and accurate processing into mature miRNAs, for native miR-like behavior. Each construct is cloned with the native stem-loop structure plus an additional 200 – 400 bps of upstream and downstream DNA, ensuring that the miRNA processing machinery operates just as it would with the native transcript.

And because our collection includes cancer and stem cell-related miRNA clusters, we offer a wider range of constructs than just the mature miRNA sequences listed in miRbase.

The precursor miRNA Expression Lentivectors drive expression of the miR precursor with the constitutive CMV promoter, for strong expression in common cell types including HeLa, HEK293, and HT1080 cell lines. Transductants/transfectants can be easily sorted using copGFP.

  • Transfect or transduce the precursor miRNA lentivectors
  • Create stably-expressing miR cell lines
  • Leverage SBI’s highly-regarded lentivectors
  • Obtain accurate miR processing and efficient expression through the use of native precursor sequences
  • Use copGFP and puromycin markers to identify desired clones

How it Works

SBI’s Human pre-miRNA Expression Lentivectors use the native miRNA processing machinery

Supporting Data

Get high levels of miRs from our Human pre-miRNA Expression Lentivectors

Lenti-miR-205 is expressed at high levels in MCF-7 cells

Lenti-miRs are overexpressed in HEK293 cells

Lenti-miR stem cell cluster constructs are overexpressed

 

Citations

  • Winston, C, et al. (2019) Complement protein levels in plasma astrocyte-derived exosomes are abnormal in conversion from mild cognitive impairment to Alzheimer’s disease dementia. Alzheimer's & Dementia: Diagnosis, Assessment & Disease Monitoring. 2019 Dec 1; 11:61-66. Link: Alzheimer's & Dementia: Diagnosis, Assessment & Disease Monitoring
  • Kim, Y & Mok, H. (2019) Citraconylated exosomes for improved internalization into macrophages. Appl Biol Chem. 2019 Dec 1; 62(1). Link: Appl Biol Chem
  • Saunders, CA, et al. (2019) Genetic manipulation of PLB-985 cells and quantification of chemotaxis using the underagarose assay. Methods Cell Biol.. 2019 Oct 29; 149:31-56. PM ID: 30616826
  • Sekiba, K, et al. (2019) Inhibition of HBV Transcription From cccDNA With Nitazoxanide by Targeting the HBx-DDB1 Interaction. Cell Mol Gastroenterol Hepatol. 2019 Oct 24; 7(2):297-312. PM ID: 30704981
  • Yang, JS, et al. (2019) Investigation of lipidomic perturbations in oxidatively stressed subcellular organelles and exosomes by asymmetrical flow field-flow fractionation and nanoflow ultrahigh performance liquid chromatography-tandem mass spectrometry. Anal. Chim. Acta. 2019 Sep 27; 1073:79-89. PM ID: 31146839
  • Ishihara, R, et al. (2019) Design of a surface-functionalized power-free microchip for extracellular vesicle detection utilizing UV grafting. Reactive and Functional Polymers. 2019 Sep 1; 142:183-188. Link: Reactive and Functional Polymers
  • Gamboa, L, et al. (2019) Heat-triggered remote control of CRISPR-dCas9 for tunable transcriptional modulation. bioRxiv. 2019 Aug 14;. Link: bioRxiv
  • Xu, Q, et al. (2019) miR‑132 inhibits high glucose‑induced vascular smooth muscle cell proliferation and migration by targeting E2F5. Mol Med Rep. 2019 Aug 1; 20(2):2012-2020. PM ID: 31257477
  • Liu, L, et al. (2019) IL‑17A promotes CXCR2‑dependent angiogenesis in a mouse model of liver cancer. Mol Med Rep. 2019 Aug 1; 20(2):1065-1074. PM ID: 31173199
  • Hisano, Y, et al. (2019) Lysolipid receptor cross-talk regulates lymphatic endothelial junctions in lymph nodes. J. Exp. Med.. 2019 Jul 1; 216(7):1582-1598. PM ID: 31147448
  • Choi, YW, et al. (2019) Abrogation of B-RafV600E induced senescence by FoxM1 expression. Biochem. Biophys. Res. Commun.. 2019 Jun 30;. PM ID: 31270027
  • Madhu, LN, et al. (2019) Neuroinflammation in Gulf War Illness is linked with HMGB1 and complement activation, which can be discerned from brain-derived extracellular vesicles in the blood. Brain Behav. Immun.. 2019 Jun 27;. PM ID: 31255677
  • Amado, F, et al. (2019) Sample Treatment for Saliva Proteomics. Adv. Exp. Med. Biol.. 2019 Jun 26; 1073:23-56. PM ID: 31236838
  • Tian, L, et al. (2019) Long-read sequencing unveils IGH-DUX4 translocation into the silenced IGH allele in B-cell acute lymphoblastic leukemia. Nat Commun. 2019 Jun 26; 10(1):2789. PM ID: 31243274
  • Chen, CY, et al. (2019) Extracellular vesicles from human urine-derived stem cells prevent osteoporosis by transferring CTHRC1 and OPG. Bone Res. 2019 Jun 26; 7:18. PM ID: 31263627
  • Zhang, L & Li, D. (2019) MORC2 regulates DNA damage response through a PARP1-dependent pathway. Nucleic Acids Research. 2019 Jun 25;. Link: Nucleic Acids Research
  • Ariyoshi, K, et al. (2019) Radiation-Induced Bystander Effect is Mediated by Mitochondrial DNA in Exosome-Like Vesicles. Sci Rep. 2019 Jun 24; 9(1):9103. PM ID: 31235776
  • Lee, H, et al. (2019) Caveolin-1 selectively regulates microRNA sorting into microvesicles after noxious stimuli. J. Exp. Med.. 2019 Jun 24;. PM ID: 31235510
  • Zhao, J, et al. (2019) Tumor-Derived Extracellular Vesicles Inhibit Natural Killer Cell Function in Pancreatic Cancer. Cancers (Basel). 2019 Jun 22; 11(6). PM ID: 31234517
  • Wang, X, et al. (2019) Prostate carcinoma cell-derived exosomal MicroRNA-26a modulates the metastasis and tumor growth of prostate carcinoma. Biomed. Pharmacother.. 2019 Jun 20; 117:109109. PM ID: 31229922

Have Questions?

A System Biosciences technical expert is happy to help!

(888) 266-5066 or Contact Us

Sign up to receive technical advice and exclusive deals directly to your inbox.