Anti-FLOT1 Antibody with Goat Anti-Rabbit HRP Secondary Antibody
- Rigorously validated
- Hand-picked selection
- Expert exosome support
Products
| Catalog Number | Description | Size | Price | Quantity | Add to Cart | |||
|---|---|---|---|---|---|---|---|---|
| EXOAB-FLOT1-1 | Anti-FLOT1 Antibody (rabbit anti-human, mouse) with goat anti-rabbit HRP secondary antibody | 25 µL | $171 |
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Overview
Overview
Confirm exosome isolation with confidence
When you’re ready to confirm that you’ve isolated exosomes, stick with the exosome experts at SBI. Our polyclonal rabbit anti-FLOT1 antibodies recognize the human and mouse forms of the protein, and are a great choice for general exosome detection, especially when used in conjunction with antibodies for other exosome markers such as the tetraspanins CD9, CD63, and CD81, as well as the heat shock protein HSP70 (if you have any questions about the best antibodies to use for your exosome project, we’re ready to help! Just email us at tech@systembio.com). And, like all of our antibodies, our anti-FLOT1 antibody and the included goat anti-rabbit HRP secondary antibodies are rigorously validated for exosome detection via Western blotting.
- Rigorously validated
- Hand-picked selection
- Expert exosome support
Choose the right antibody for your exosome detection needs
| Cat.# | Antibody to | Notes | Link to ExoCarta entry for the human protein |
|---|---|---|---|
| General exosome markers | |||
| EXOAB-CD9A-1 | CD9 | Tetraspanin | ExoCarta_928 |
| EXOAB-CD63A-1 | CD63 | Tetraspanin | ExoCarta_967 |
| EXOAB-CD81A-1 | CD81 | Tetraspanin | ExoCarta_93185 |
| EXOAB-Hsp70A-1 | HSP70 | ExoCarta_3303, ExoCarta_3304, ExoCarta_3306, ExoCarta_3308, ExoCarta 3310, ExoCarta_3311 | |
| EXOAB-ANXA5-1 | ANXA5-1 | Annexin A5 | ExoCarta_308 |
| EXOAB-TSG101-1 | TSG101 | Tumor susceptibility gene 101 | ExoCarta_7251 |
| EXOAB-FLOT1-1 | FLOT-1 | Flotillin-1 | ExoCarta_10211 |
| Tissue-specific exosome markers | |||
| EXOAB-CXCR4-1 | CXCR4 | Specific for B-cells, T-cells, and platelets | ExoCarta_7852 |
| EXOAB-EPCAM1-1 | EpCam | Specific for cancer-derived exosomes | ExoCarta_4072 |
| EXOAB-VMTN-1 | Vimentin | Specific for mesenchymal stem cells | ExoCarta_7431 |
References
How It Works
Supporting Data
Supporting Data
See isolation data using Exo-FLOW IP Kits
For these studies, either human serum or HEK293 exosomes concentrated from cell culture media using ExoQuick-TC were added to the antibody-coupled magnetic beads. After washing, exosomes were eluted and recovery estimated using a standard BCA protein assay.
Figure 1. CD63 and CD9, two exosome markers, are readily detected in samples purified using Exo-Flow Kits. Approximately 1 µg of protein was loaded per well on a 4-20% gradient protein PAGE. The proteins were separated and transferred to nitrocellulose membranes for Western blot analysis. The blots were probed with either with anti-CD63 or anti-CD9 antibodies to detect the exosome protein markers.
Figure 2. NanoSight analysis shows Exo-Flow IP Kits deliver good yields of particles whose sizes are consistent with exosomes.
FAQs
Resources
Citations
Related Products
Products
| Catalog Number | Description | Size | Price | Quantity | Add to Cart | |||
|---|---|---|---|---|---|---|---|---|
| EXOAB-FLOT1-1 | Anti-FLOT1 Antibody (rabbit anti-human, mouse) with goat anti-rabbit HRP secondary antibody | 25 µL | $171 |
|
||||
Overview
Overview
Confirm exosome isolation with confidence
When you’re ready to confirm that you’ve isolated exosomes, stick with the exosome experts at SBI. Our polyclonal rabbit anti-FLOT1 antibodies recognize the human and mouse forms of the protein, and are a great choice for general exosome detection, especially when used in conjunction with antibodies for other exosome markers such as the tetraspanins CD9, CD63, and CD81, as well as the heat shock protein HSP70 (if you have any questions about the best antibodies to use for your exosome project, we’re ready to help! Just email us at tech@systembio.com). And, like all of our antibodies, our anti-FLOT1 antibody and the included goat anti-rabbit HRP secondary antibodies are rigorously validated for exosome detection via Western blotting.
- Rigorously validated
- Hand-picked selection
- Expert exosome support
Choose the right antibody for your exosome detection needs
| Cat.# | Antibody to | Notes | Link to ExoCarta entry for the human protein |
|---|---|---|---|
| General exosome markers | |||
| EXOAB-CD9A-1 | CD9 | Tetraspanin | ExoCarta_928 |
| EXOAB-CD63A-1 | CD63 | Tetraspanin | ExoCarta_967 |
| EXOAB-CD81A-1 | CD81 | Tetraspanin | ExoCarta_93185 |
| EXOAB-Hsp70A-1 | HSP70 | ExoCarta_3303, ExoCarta_3304, ExoCarta_3306, ExoCarta_3308, ExoCarta 3310, ExoCarta_3311 | |
| EXOAB-ANXA5-1 | ANXA5-1 | Annexin A5 | ExoCarta_308 |
| EXOAB-TSG101-1 | TSG101 | Tumor susceptibility gene 101 | ExoCarta_7251 |
| EXOAB-FLOT1-1 | FLOT-1 | Flotillin-1 | ExoCarta_10211 |
| Tissue-specific exosome markers | |||
| EXOAB-CXCR4-1 | CXCR4 | Specific for B-cells, T-cells, and platelets | ExoCarta_7852 |
| EXOAB-EPCAM1-1 | EpCam | Specific for cancer-derived exosomes | ExoCarta_4072 |
| EXOAB-VMTN-1 | Vimentin | Specific for mesenchymal stem cells | ExoCarta_7431 |
References
How It Works
Supporting Data
Supporting Data
See isolation data using Exo-FLOW IP Kits
For these studies, either human serum or HEK293 exosomes concentrated from cell culture media using ExoQuick-TC were added to the antibody-coupled magnetic beads. After washing, exosomes were eluted and recovery estimated using a standard BCA protein assay.
Figure 1. CD63 and CD9, two exosome markers, are readily detected in samples purified using Exo-Flow Kits. Approximately 1 µg of protein was loaded per well on a 4-20% gradient protein PAGE. The proteins were separated and transferred to nitrocellulose membranes for Western blot analysis. The blots were probed with either with anti-CD63 or anti-CD9 antibodies to detect the exosome protein markers.
Figure 2. NanoSight analysis shows Exo-Flow IP Kits deliver good yields of particles whose sizes are consistent with exosomes.