AAVS1 Safe Harbor Targeting

CMV-hspCas9-T2A-Puro-H1-AAVS1_gRNA All-in-one Cas9 SmartNuclease™ & AAVS1 gRNA Lentivector

Streamline genome editing at the AAVS1 Safe Harbor Site in hard-to-transfect cells with a lentivector that expresses both Cas9 and an AAVS1-targeting gRNA

Sizes:10 µg (Cat. # CASLV601PA-1), 2 x 25 µL (Cat. # CASLV601VA-1)

All-in-one Cas9 SmartNuclease™ & AAVS1 gRNA Plasmid

Streamline genome editing at the AAVS1 Safe Harbor Site with a plasmid that expresses both Cas9 and a gRNA that targets the AAVS1 site

Size:10 µg (Cat. # CAS601A-1)

DIY Cas9-expressing Cells HR Donor

Streamline gRNA screening, functional validation and more—make your own Cas9-expressing cell lines with this HR donor that knocks-in Cas9 to the AAVS1 site.

Size:10 µg (Cat. # CAS620A-1)

DIY Cas9-expressing Cells Complete Kit

Streamline gRNA screening, functional validation and more—get everything you need to make your own cell lines expressing Cas9 from the AAVS1 site.

Size:1 Kit (Cat. # CAS620A-KIT)

AAVS1 Safe Harbor Site Targeting Knock-in HR Donor Vector 2.0 (AAVS1-SA-puro-EF1α-MCS)

Easily knock-in any gene into the AAVS1 Safe Harbor Site—your gene-of-interest is driven by the constitutive EF1α promoter

Size:10 µg (Cat. # GE622A-1)

AAVS1 Safe Harbor Site Targeting 2.0 Complete Kit with All-purpose HR Donor Vector 2.0 (AAVS1-SA-puro-EF1α-MCS)

Easily knock-in and constitutively express any gene from the AAVS1 Site—includes HR Donor, All-in-one Cas9 & AAVS1 gRNA Vector, and verification primer mix

Size:1 Kit (Cat. # GE622A-KIT)

AAVS1 Safe Harbor Site Targeting Reporter Knock-in HR Donor Vector 2.0 (AAVS1-SA-puro-MCS-GFP)

Easily create reporter cell lines that express GFP from your promoter at the AAVS1 Safe Harbor Site—clone your promoter into the MCS upstream of GFP

Size:10 µg (Cat. # GE624A-1)

AAVS1 Safe Harbor Site Targeting 2.0 Complete Kit with All-purpose HR Donor Vector 2.0 (AAVS1-SA-puro-MCS-GFP)

Easily create reporter cell lines that express GFP from the AAVS1 Site—includes HR Donor, All-in-one Cas9 & AAVS1 gRNA Vector, and verification primer mix

Size:1 Kit (Cat. # GE624A-KIT)

pAAVS1D-PGK-MCS-EF1α-copGFPpuro PrecisionX™ HR Targeting Vectors

Knock-in a gene- or miRNA-of-interest into the AAVS1 site with our first-generation AAVS1 HR Targeting Vector—pAAVS1D-PGK-MCS-EF1α-copGFPpuro

Size:10 µg (Cat. # GE602A-1)

pAAVS1D-CMV-RFP-EF1α-copGFPpuro PrecisionX™ HR Targeting Vector

Knock-in RFP into the AAVS1 site with our first-generation positive control AAVS1 HR Targeting Vector—pAAVS1D-CMV-RFP-EF1α-copGFPpuro

Size:10 µg (Cat. # GE603A-1)

AAVS1 Safe Harbor Site Targeting 2.0 Complete Kit with All-purpose HR Donor Vector 2.0 (AAVS1-SA-puro-MCS)

Easily knock-in any gene or promoter-gene combination into the AAVS1 Site—includes HR Donor, All-in-one Cas9 & AAVS1 gRNA Vector, and verification primer mix

Size:1 Kit (Cat. # GE620A-KIT)

AAVS1 Safe Harbor Site Targeting All-purpose HR Donor Vector 2.0 (AAVS1-SA-puro-MCS)

Easily knock-in any gene or promoter-gene combination into the AAVS1 Safe Harbor Site

Size:10 µg (Cat. # GE620A-1)

Primer Mix to Confirm AAVS1 Integration

Easily confirm integration at the AAVS1 site with this 5’ and 3’ primer mix for Junction PCR Assays.

Size:50 Reactions (Cat. # GE640PR-1)

Primer Mix to Confirm Knock-in of AAVS1 Positive Control Donor

Confirm successful knock-in of positive control AAVS1 HR Targeting Vector pAAVS1D-CMV-RFP-EF1α-copGFPpuro with these 5’ and 3’ primers for junction PCR assays

Size:100 µL (Cat. # GE603PR-1)

Leverage the power of the AAVS1 Safe Harbor Site for robust gene expression with these HR donor vectors already targeted to the AAVS1 site