Minicircle Technology

Produced as small excised, circular DNA fragments from a parental plasmid, the non-viral, episomal Minicircle expression cassette is free of any bacterial plasmid DNA sequences, and comes with a variety of promoter and reporter combinations. Their small size facilitates more efficient transfection than what’s possible with standard-sized plasmids, and, while Minicircles do not replicate with the host cell, expression lasts for 14 days or longer in dividing cells, and can continue for months in non-dividing cells.

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Start your exosome research right with products that simplify high-quality exosome isolation

Easily detect the presence of exosomes with general and tissue-specific antibodies and arrays, as well as reagents that label exosomes

When you need to know the amount of exosomes you’ve isolated, SBI offers a range of options that meet most quantitation needs

Easily track exosomes and their cargo with our ready-to-go Cyto-Tracers, ExoGlow reagents, and XPACK products, as well as make-your-own XPACK lentivectors

Whether you’re interested in characterizing exosomal RNA or proteins for biomarker profiling, SBI offers a range of intuitive, easy-to-use products

When you’re ready to move from simply studying exosomes to using them as research tools, SBI offers a complete set of products for exosome engineering

Find the best Cas9 and gRNA delivery options for your genome engineering application—choose from mRNA, lentivectors, protein, AAV, and more

Increase the efficiency of making multiple changes to a genome with our Multiplex gRNA Cloning Products

Move your genome engineering projects forward with confidence with products for verifying Cas9 mRNA and protein expression

Whether you’re using CRISPR/Cas9 for gene knock-ins, knock-outs, or edits, SBI’s ready-to-clone HR Targeting Vectors are carefully designed for easier workflows

Leverage the power of the AAVS1 Safe Harbor Site for robust gene expression with these HR donor vectors already targeted to the AAVS1 site

Fast, streamlined genotyping of cultured mammalian cells using a protocol that mitigates the risk of sample cross-contamination

Explore a new frontier in the study of gene expression regulation with inducible, reversible chromatin loop formation at your location-of-interest

Targeted Protein Degradation Products

New to lentivirus or scaling up production? Our LentiSuite and LentiStarter Kits provide all of SBI’s great lentivirus products in ready-to-go kits

Get high-quality, high-titer lentivirus with our well-regarded lentivirus packaging systems, available in both integrating and non-integrating systems

Find what you need for fast lentivirus titering as well as lentivirus concentration

Improve transduction and transfection efficiencies with high-quality reagents that really deliver

Get the confidence of controls and move forward fast with SBI’s ready-to-transduce, pre-packaged control viruses—in a range of marker and promoter options

Get reliable, stable gene expression from SBI’s highly-regarded lentivectors—our wide range of promoters and reporters support a wealth of applications

Get the efficiency of transduction without viral integration with our AAV-based expression systems—great for gene therapy research and more

For the ultimate in gene expression control, use SBI’s cumate-inducible systems—up to 32-fold induction that’s titratable, reversible, and background-free

Easy, consistent transgenesis free from limitations on insert size and available in a footprint-free format

When you want non-viral expression from low or single-copy integration events, and/or have a large insert size, choose one of SBI’s integrase-based systems

Get sustained, episomal gene expression free from foreign DNA—great for model animal generation and gene therapy research

An easy-to-produce non-integrating option for gene expression that can deliver sustained expression from a non-viral system

From an efficient system for in vitro transcription to ready-to-transfect pre-made mRNA transcripts, SBI streamlines your mRNA production

The Cold Fusion technology is a simple, rapid and highly efficient cloning kit

Simplify your studies of T cell dynamics with SBI’s ready-to-use, pre-built Treg and Th17 vectors and cell lines

Targeted Protein Degradation System

Easily follow signal transduction with our pre-built, ready-to-go vectors that provide qualitative and quantitative data on a variety of signaling pathways

Clearly visualize cells in vivo and ex vivo for tracking cell fate and more with our pre-built imaging vectors

Jumpstart your studies of dynamic cellular processes and subcellular localization with our pre-built Cyto-Tracer Vectors

Save time with our ready-to-go pGreenFire, pGreenZeo, pRedZeo, and pRedTK vectors for monitoring pluripotency and tracking differentiation

Simplify your studies of T cell dynamics with SBI’s ready-to-use, pre-built Treg and Th17 vectors and cell lines

Alter gene expression or study miR function with human and mouse lentivector-delivered precursors that are efficiently and accurately processed into mature miRs

Leverage SBI’s powerful and highly-effective Lenti-miR technology for efficient miRNA screening with our pooled Lenti-miR libraries

Permanently knockdown miRs with SBI’s miRZip anti-miRNA Expression Lentivectors

Quickly and easily obtain highly accurate qPCR profiling of miRNAs with sample prep kits and ready-to-use arrays

Quickly and easily obtain highly accurate qPCR profiling of lncRNAs with sample prep kits and ready-to-use arrays

Find the SBI product or service that supports your specific research application

SBI can streamline your research with an array of custom services to suit your needs

Get in-depth information about SBI’s products and services with videos, webinars, product manuals, and more

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Minicircle Technology

MC-Easy Minicircle DNA Production Kit

MC-Easy Minicircle DNA Production Kit with Competent Cells

pMC.CMV-MCS-EF1α-GFP-SV40polyA Parental Minicircle Cloning Vector

pMC.EF1α-MCS-SV40polyA Parental Minicircle Cloning Vector

ZYCY10P3S2T Minicircle Production Strain (Competent Cells)

Mouse IL-23 Pre-made Minicircle DNA (RSV->FLAG-mP40-mP19-pA)

Resources

Sustained Transgene Expression using Minicircles Webinar

PiggyBac for Easy, Consistent Transgenesis Webinar

Minicircle DNA Breathes Life into Gene Therapy Development

Fast, Efficient Molecular Cloning to Generate Constructs

Cumate-Inducible Gene Expression

Minicircle Production Custom Service

Minicircle Technology

Product Filters

MC-Easy™ Minicircle DNA Production Kit (with Competent Cells)

MC-Easy™ Bonus Media Pack

Kanamycin Solution

Arabinose Induction Solution

ZYCY10P3S2T Minicircle Production Strain (Competent Cells)

MC-Easy™ Minicircle DNA Production Kit (without Competent Cells)

pMC.CMV-GFP-SV40PolyA Parental Minicircle Positive Control Plasmid Vector

pMC.CMV-GFP-SV40PolyA Pre-made Positive Control Minicircle

pMC.CMV-RFP-SV40PolyA Parental Minicircle Positive Control Plasmid Vector

pMC.CMV-RFP-SV40PolyA Pre-made Positive Control Minicircle

pMC.BESPX-MCS2 Empty Parental Minicircle Cloning Vector

pMC.CMV-MCS-SV40polyA Parental Minicircle Cloning Vector

pMC.EF1α-MCS-SV40polyA Parental Minicircle Cloning Vector

pMC.CMV-MCS-EF1α-GFP-SV40polyA Parental Minicircle Cloning Vector

pMC.CMV-MCS-EF1α-RFP-SV40polyA Parental Minicircle Cloning Vector

pMC.EF1α-MCS-IRES-GFP-SV40polyA Parental Minicircle Cloning Vector

pMC.EF1α-MCS-IRES-RFP-SV40polyA Parental Minicircle Cloning Vector

Mouse IL-23 Pre-made Minicircle DNA (RSV->FLAG-mP40-mP19-pA)

pMC.BESPX-MCS1 Empty Parental Minicircle Cloning Vector

pMC.CMV-GFP-T2A-Puro-H1-Scramble Parental Minicircle shRNA Scramble Control Plasmid