Cumate Solution for the PiggyBac Transposon System
- Make transgenic cell lines with a single transfection
- Integrate multiple PiggyBac Vectors in a single transfection
- Insert an expression cassette into human, mouse, and rat cells
- Deliver virtually any-sized DNA insert, from 10 – 100 kb
- Choose from PiggyBac Vectors that express your gene-of-interest from constitutive or inducible promoters and include a variety of markers
Products
| Catalog Number | Description | Size | Price | Quantity | Add to Cart | |||
|---|---|---|---|---|---|---|---|---|
| PBQM100A-1 | Cumate Solution, high concentration, 10,000x for use with PiggyBac | 500 µL | $110 |
|
||||
Overview
Overview
The reagent behind tight, titratable gene expression
Prepared specifically for use with SBI’s inducible PiggyBac Vectors, the Cumate Solution for the PiggyBac Transposon System is a ready-to-add 10,000x inducer solution.
With the PiggyBac Transposon System, you can:
- Make transgenic cell lines with a single transfection
- Integrate multiple PiggyBac Vectors in a single transfection
- Insert an expression cassette into human, mouse, and rat cells
- Deliver virtually any-sized DNA insert, from 10 – 100 kb
- Choose from PiggyBac Vectors that express your gene-of-interest from constitutive or inducible promoters and include a variety of markers
- Determine the number of integration events with the PiggyBac qPCR Copy Number Kit (# PBC100A-1)
Customer Agreements Academic customers can purchase PiggyBac Transposon System components for internal research purposes for indefinite use, whereas commercial customers must sign a customer agreement for a six-month, limited-use license to evaluate the technology.
* SBI is fully licensed to distribute PiggyBac vectors as a partnership with Hera BioLabs, Inc.
References
How It Works
How It Works
Tightly-controlled, inducible gene expression
Get robust, titratable gene expression with low background using SBI’s cumate-inducible vectors. These vectors take advantage of CymR, a repressor that binds to cumate operator sequences (CuO) with high affinity in the absence of cumate, a non-toxic small molecule. Providing much lower background expression than similar systems, SBI’s cumate-inducible vectors can provide up to 32-fold induction of gene expression.
- Robust—increase expression up to 32-fold
- Adjustable—tune expression levels by titrating the amount of cumate
- Reversible—turn expression on, then off, then on again
- Powerful—suitable for in vivo applications
Supporting Data
Supporting Data
Tight expression control with low background
Figure 2. In the absence of cumate, the cumate-inducible PiggyBac Vector shows undetectable levels of expression.
Figure 3. The PiggyBac cumate switch is titratable and can be turned off.
FAQs
Resources
Citations
Related Products
Products
| Catalog Number | Description | Size | Price | Quantity | Add to Cart | |||
|---|---|---|---|---|---|---|---|---|
| PBQM100A-1 | Cumate Solution, high concentration, 10,000x for use with PiggyBac | 500 µL | $110 |
|
||||
Overview
Overview
The reagent behind tight, titratable gene expression
Prepared specifically for use with SBI’s inducible PiggyBac Vectors, the Cumate Solution for the PiggyBac Transposon System is a ready-to-add 10,000x inducer solution.
With the PiggyBac Transposon System, you can:
- Make transgenic cell lines with a single transfection
- Integrate multiple PiggyBac Vectors in a single transfection
- Insert an expression cassette into human, mouse, and rat cells
- Deliver virtually any-sized DNA insert, from 10 – 100 kb
- Choose from PiggyBac Vectors that express your gene-of-interest from constitutive or inducible promoters and include a variety of markers
- Determine the number of integration events with the PiggyBac qPCR Copy Number Kit (# PBC100A-1)
Customer Agreements Academic customers can purchase PiggyBac Transposon System components for internal research purposes for indefinite use, whereas commercial customers must sign a customer agreement for a six-month, limited-use license to evaluate the technology.
* SBI is fully licensed to distribute PiggyBac vectors as a partnership with Hera BioLabs, Inc.
References
How It Works
How It Works
Tightly-controlled, inducible gene expression
Get robust, titratable gene expression with low background using SBI’s cumate-inducible vectors. These vectors take advantage of CymR, a repressor that binds to cumate operator sequences (CuO) with high affinity in the absence of cumate, a non-toxic small molecule. Providing much lower background expression than similar systems, SBI’s cumate-inducible vectors can provide up to 32-fold induction of gene expression.
- Robust—increase expression up to 32-fold
- Adjustable—tune expression levels by titrating the amount of cumate
- Reversible—turn expression on, then off, then on again
- Powerful—suitable for in vivo applications
Supporting Data
Supporting Data
Tight expression control with low background
Figure 2. In the absence of cumate, the cumate-inducible PiggyBac Vector shows undetectable levels of expression.
Figure 3. The PiggyBac cumate switch is titratable and can be turned off.