Cytosol Cyto-Tracer™, pCT-Cyto-RFP (CMV)

Study cellular processes and physiology with this Cyto-Tracer that expresses un-tagged RFP from the CMV promoter - untagged RFP localizes in the cytosol
  • Stable lentivector-based system
  • Great for creating stable reporter cell lines
  • Ideal for co-localization studies
  • Monitor cellular dynamics and functional studies in real time
  • Label cells with RFP for a broad range of studies

Products

Catalog Number Description Size Price Quantity Add to Cart
CYTO119-PA-1 pCT-Cyto-RFP (pCMV, Cytosol, Untagged) 10 µg $643
- +
CYTO119-VA-1 pCT-Cyto-RFP (pCMV, Cytosol, Untagged; pre-packaged virus) >2x10^6 IFUs $741
- +

Overview

Overview

Easily study the dynamics of cellular processes with SBI’s Cyto-Tracers™

Leverage SBI’s high-quality lentivector technology to study subcellular localization and dynamics with our line of Cyto-Tracer vectors. Easily create stable reporter cell lines with our validated, pre-built lentivectors—available as transfectable plasmids or ready-to-transduce, pre-packaged virus. The Cytosol Cyto-Tracer, pCT-Cyto-RFP (CMV), expresses an untagged RFP, which naturally localizes to the cytosol. RFP is expressed from the CMV promoter for strong expression in common cell types such as HeLa, HEK293, and HT1080.
  • Stable lentivector-based system
  • Great for creating stable reporter cell lines
  • Ideal for co-localization studies
  • Monitor cellular dynamics and functional studies in real time
  • Label cells with RFP for a broad range of studies
Choose the Cyto-Tracer that’s right for you
Promoter (Cat.#)Cyto-TracerTarget LocationPeptide Tag
CMV (CYTO100-PA-1/CYTO100-VA-1)
MSCV (CYTO100-PB-1/CYTO100-VB-1)
pCT-Mem-GFPPlasma MembraneNeuromodulin
CMV (CYTO101-PA-1/CYTO101-VA-1)
MSCV (CYTO101-PB-1/CYTO101-VB-1)
pCT-InnerMem-GFPInner Leaflet of MembraneFarnesylation signal
CMV (CYTO102-PA-1/CYTO102-VA-1)
MSCV (CYTO102-PB-1/CYTO102-VB-1)
pCT-Mito-GFPMitochondriaCytochrome C oxidase VIII
CMV (CYTO103-PA-1/CYTO103-VA-1)pCT-ER-GFPEndoplasmic ReticulumER targeting consensus
CMV (CYTO104-PA-1/CYTO104-VA-1)pCT-Golgi-GFPGolgi ApparatusGalactosyltrasferase
CMV (CYTO105-PA-1/CYTO105-VA-1)pCT-Secretory-GFPER-Golgi  VesiclesSecretory consensus peptide
CMV (CYTO106-PA-1/CYTO106-VA-1)pCT-Lyso-GFPLysosomeCathepsin B
CMV (CYTO107-PA-1/CYTO107-VA-1)pCT-Endo-GFPEndosomeRhoB
CMV (CYTO107R-PA-1/CYTO107R-VA-1)pCT-Endo-RFPEndosomeRhoB
CMV (CYTO108-PA-1/CYTO108-VA-1)
MSCV (CYTO108-PB-1/CYTO108-VB-1)
pCT-Pero-GFPPeroxisomePeroxisomal consensus signal
CMV (CYTO109-PA-1/CYTO109-VA-1)pCT-Autophago-GFP
AutophagosomeLC3
CMV (CYTO109R-PA-1/CYTO109R-VA-1)pCT-Autophago-RFPAutophagosomeLC3
CMV (CYTO120-PA-1/CYTO120-VA-1)pCT-CD63-GFPExosome/SecretoryCD63 Tetraspanin
CMV (CYTO120R-PA-1/CYTO120R-VA-1)pCT-CD63-RFPExosome/SecretoryCD63 Tetraspanin
CMV (CYTO122-PA-1/CYTO122-VA-1)pCT-CD9-GFPExosome/SecretoryCD9 Tetraspanin
CMV (CYTO123-PA-1/CYTO123-VA-1)pCT-CD9-RFPExosome/SecretoryCD9 Tetraspanin
CMV (CYTO124-PA-1/CYTO124-VA-1)pCT-CD81-GFPExosome/SecretoryCD81 Tetraspanin
CMV (CYTO125-PA-1/CYTO125-VA-1)pCT-CD81-RFPExosome/SecretoryCD81 Tetraspanin
CMV (CYTO110-PA-1/CYTO110-VA-1)
MSCV (CYTO110-PB-1/CYTO110-VB-1)
pCT-Actin-GFPCytoskeletonβ-actin
CMV (CYTO111-PA-1/CYTO111-VA-1)
MSCV (CYTO111-PB-1/CYTO111-VB-1)
pCT-Tublin-GFPCytoskeletonα-tublin
CMV (CYTO111R-PA-1/CYTO111R-VA-1)pCT-Tublin-RFPCytoskeletonα-tublin
CMV (CYTO112-PA-1/CYTO112-VA-1)pCT-MAP4-GFPMicrotubuleMicrotubule associated protein 4 (MAP4)
CMV (CYTO113-PA-1/CYTO113-VA-1)
MSCV (CYTO113-PB-1/CYTO113-VB-1)
pCT-H2B-GFPNucleusH2B
CMV (CYTO114-PA-1/CYTO114-VA-1)pCT-Apoptosis-Luc Caspase 3/7 activationCyclic inactive luciferase
CMV (CYTO115-PA-1/CYTO115-VA-1)pCT-GFP-BAXCytosol to MitochondriaBAX
CMV (CYTO116-PA-1/CYTO116-VA-1)pCT- Catenin-GFPCytosol to Nucleus/Cell Membraneβ-Catenin activation
CMV (CYTO117-PA-1/CYTO117-VA-1)pCT-CMV-PSD95-GFPDendrite MembranesPSD-95
CMV (CYTO118-PA-1/CYTO118-VA-1)
MSCV (CYTO118-PB-1/CYTO118-VB-1)
pCT-Cyto-GFPCytosolic CompartmentGFP-Untagged
CMV (CYTO119-PA-1/CYTO119-VA-1)pCT-Cyto-RFPCytosolic CompartmentRFP-Untagged
MSCV (CYTO121-PB-1/CYTO121-VB-1)pCT-Cyto-GFpLucGFP and Luciferase ControlGFP and Luciferase (Untagged)

References

How It Works

How It Works

Visualizing the cell with Cyto-Tracers

SBI’s Cyto-Tracers fuse a specific protein tag to copGFP or RFP, directing the fluorescent protein to the desired subcellular location.

SBI’s Cyto-Tracers fuse a specific protein tag to copGFP or RFP, directing the fluorescent protein to the desired subcellular location

Supporting Data

Supporting Data

See some of our Cyto-Tracers in action

SBI’s Cyto-Tracers in action

FAQs

Resources

Citations

Products

Catalog Number Description Size Price Quantity Add to Cart
CYTO119-PA-1 pCT-Cyto-RFP (pCMV, Cytosol, Untagged) 10 µg $643
- +
CYTO119-VA-1 pCT-Cyto-RFP (pCMV, Cytosol, Untagged; pre-packaged virus) >2x10^6 IFUs $741
- +

Overview

Overview

Easily study the dynamics of cellular processes with SBI’s Cyto-Tracers™

Leverage SBI’s high-quality lentivector technology to study subcellular localization and dynamics with our line of Cyto-Tracer vectors. Easily create stable reporter cell lines with our validated, pre-built lentivectors—available as transfectable plasmids or ready-to-transduce, pre-packaged virus. The Cytosol Cyto-Tracer, pCT-Cyto-RFP (CMV), expresses an untagged RFP, which naturally localizes to the cytosol. RFP is expressed from the CMV promoter for strong expression in common cell types such as HeLa, HEK293, and HT1080.
  • Stable lentivector-based system
  • Great for creating stable reporter cell lines
  • Ideal for co-localization studies
  • Monitor cellular dynamics and functional studies in real time
  • Label cells with RFP for a broad range of studies
Choose the Cyto-Tracer that’s right for you
Promoter (Cat.#)Cyto-TracerTarget LocationPeptide Tag
CMV (CYTO100-PA-1/CYTO100-VA-1)
MSCV (CYTO100-PB-1/CYTO100-VB-1)
pCT-Mem-GFPPlasma MembraneNeuromodulin
CMV (CYTO101-PA-1/CYTO101-VA-1)
MSCV (CYTO101-PB-1/CYTO101-VB-1)
pCT-InnerMem-GFPInner Leaflet of MembraneFarnesylation signal
CMV (CYTO102-PA-1/CYTO102-VA-1)
MSCV (CYTO102-PB-1/CYTO102-VB-1)
pCT-Mito-GFPMitochondriaCytochrome C oxidase VIII
CMV (CYTO103-PA-1/CYTO103-VA-1)pCT-ER-GFPEndoplasmic ReticulumER targeting consensus
CMV (CYTO104-PA-1/CYTO104-VA-1)pCT-Golgi-GFPGolgi ApparatusGalactosyltrasferase
CMV (CYTO105-PA-1/CYTO105-VA-1)pCT-Secretory-GFPER-Golgi  VesiclesSecretory consensus peptide
CMV (CYTO106-PA-1/CYTO106-VA-1)pCT-Lyso-GFPLysosomeCathepsin B
CMV (CYTO107-PA-1/CYTO107-VA-1)pCT-Endo-GFPEndosomeRhoB
CMV (CYTO107R-PA-1/CYTO107R-VA-1)pCT-Endo-RFPEndosomeRhoB
CMV (CYTO108-PA-1/CYTO108-VA-1)
MSCV (CYTO108-PB-1/CYTO108-VB-1)
pCT-Pero-GFPPeroxisomePeroxisomal consensus signal
CMV (CYTO109-PA-1/CYTO109-VA-1)pCT-Autophago-GFP
AutophagosomeLC3
CMV (CYTO109R-PA-1/CYTO109R-VA-1)pCT-Autophago-RFPAutophagosomeLC3
CMV (CYTO120-PA-1/CYTO120-VA-1)pCT-CD63-GFPExosome/SecretoryCD63 Tetraspanin
CMV (CYTO120R-PA-1/CYTO120R-VA-1)pCT-CD63-RFPExosome/SecretoryCD63 Tetraspanin
CMV (CYTO122-PA-1/CYTO122-VA-1)pCT-CD9-GFPExosome/SecretoryCD9 Tetraspanin
CMV (CYTO123-PA-1/CYTO123-VA-1)pCT-CD9-RFPExosome/SecretoryCD9 Tetraspanin
CMV (CYTO124-PA-1/CYTO124-VA-1)pCT-CD81-GFPExosome/SecretoryCD81 Tetraspanin
CMV (CYTO125-PA-1/CYTO125-VA-1)pCT-CD81-RFPExosome/SecretoryCD81 Tetraspanin
CMV (CYTO110-PA-1/CYTO110-VA-1)
MSCV (CYTO110-PB-1/CYTO110-VB-1)
pCT-Actin-GFPCytoskeletonβ-actin
CMV (CYTO111-PA-1/CYTO111-VA-1)
MSCV (CYTO111-PB-1/CYTO111-VB-1)
pCT-Tublin-GFPCytoskeletonα-tublin
CMV (CYTO111R-PA-1/CYTO111R-VA-1)pCT-Tublin-RFPCytoskeletonα-tublin
CMV (CYTO112-PA-1/CYTO112-VA-1)pCT-MAP4-GFPMicrotubuleMicrotubule associated protein 4 (MAP4)
CMV (CYTO113-PA-1/CYTO113-VA-1)
MSCV (CYTO113-PB-1/CYTO113-VB-1)
pCT-H2B-GFPNucleusH2B
CMV (CYTO114-PA-1/CYTO114-VA-1)pCT-Apoptosis-Luc Caspase 3/7 activationCyclic inactive luciferase
CMV (CYTO115-PA-1/CYTO115-VA-1)pCT-GFP-BAXCytosol to MitochondriaBAX
CMV (CYTO116-PA-1/CYTO116-VA-1)pCT- Catenin-GFPCytosol to Nucleus/Cell Membraneβ-Catenin activation
CMV (CYTO117-PA-1/CYTO117-VA-1)pCT-CMV-PSD95-GFPDendrite MembranesPSD-95
CMV (CYTO118-PA-1/CYTO118-VA-1)
MSCV (CYTO118-PB-1/CYTO118-VB-1)
pCT-Cyto-GFPCytosolic CompartmentGFP-Untagged
CMV (CYTO119-PA-1/CYTO119-VA-1)pCT-Cyto-RFPCytosolic CompartmentRFP-Untagged
MSCV (CYTO121-PB-1/CYTO121-VB-1)pCT-Cyto-GFpLucGFP and Luciferase ControlGFP and Luciferase (Untagged)

References

How It Works

How It Works

Visualizing the cell with Cyto-Tracers

SBI’s Cyto-Tracers fuse a specific protein tag to copGFP or RFP, directing the fluorescent protein to the desired subcellular location.

SBI’s Cyto-Tracers fuse a specific protein tag to copGFP or RFP, directing the fluorescent protein to the desired subcellular location

Supporting Data

Supporting Data

See some of our Cyto-Tracers in action

SBI’s Cyto-Tracers in action

FAQs

Citations