EV-LuminiteTM
- Accurate Luminous Quantification
- High Sensitivity and Reliability
- Stable Lenti-based System
- Enhanced Visibility into Subpopulation EV Processes
- Customizable Applications High-Content Screening (HCS) Applications
- Advanced Research and Therapeutic Potential
Products
| Catalog Number | Description | Size | Price | Quantity | Add to Cart | |||
|---|---|---|---|---|---|---|---|---|
| EVLT-CD9-PA-P | EV-LuminiteTM pCDH-MSCV-NLuc-CD9-EF1-Puro Expression Lentivector | 10 µg | $789 |
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| EVLT-CD9-PA-B | EV-LuminiteTM pCDH-MSCV-NLuc-CD9- EF1-Blast Expression Lentivector | 10 µg | $789 |
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| EVLT-CD63-PA-B | EV-LuminiteTM pCDH-MSCV-NLuc-CD63- EF1-Blast Expression Lentivector | 10 µg | $789 |
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| EVLT-CD63-PA-P | EV-LuminiteTM pCDH-MSCV-NLuc-CD63- EF1-Puro Expression Lentivector | 10 µg | $789 |
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| EVLT-CD81-PA-P | EV-LuminiteTM pCDH-MSCV-NLuc-CD81- EF1-Puro Expression Lentivector | 10 µg | $789 |
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| EVLT-CD81-PA-B | EV-LuminiteTM pCDH-MSCV-NLuc-CD81- EF1-Blast Expression Lentivector | 10 µg | $789 |
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| EVLT-CD9-VA-P | EV-LuminiteTM pCDH-MSCV-NLuc-CD9-EF1-Puro Expression Lentivirus | >1 x 10^6 IFUs | $789 |
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| EVLT-CD63-VA-P | EV-LuminiteTM pCDH-MSCV-NLuc-CD63- EF1-Puro Expression Lentivirus | >1 x 10^6 IFUs | $789 |
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| EVLT-CD81-VA-P | EV-LuminiteTM pCDH-MSCV-NLuc-CD81- EF1-Puro Expression Lentivirus | >1 x 10^6 IFUs | $789 |
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| EVLT-CD9-CL-1 | EV-LuminiteTM MSCV-NLuc-CD9 HEK293 puro resistant stable cell line | 2 x 10^6 Cells | $1355 |
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| EVLT-CD63-CL-1 | EV-LuminiteTM MSCV-NLuc-CD63 HEK293 puro resistant stable cell line | 2 x 10^6 Cells | $1355 |
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| EVLT-CD81-CL-1 | EV-LuminiteTM MSCV-NLuc-CD81 HEK293 puro resistant stable cell line | 2 x 10^6 Cells | $1355 |
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| EVLT-CD9-EXOP-1 | Exosomes from EV-LuminiteTM pCDH-MSCV-NLuc-CD9 HEK293 puro resistant stable cell line | 50 µg | $498 |
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| EVLT-CD63-EXOP-1 | Exosomes from EV-LuminiteTM pCDH-MSCV-NLuc-CD63 HEK293 puro resistant stable cell line | 50 µg | $498 |
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| EVLT-CD81-EXOP-1 | Exosomes from EV-LuminiteTM pCDH-MSCV-NLuc-CD81 HEK293 puro resistant stable cell line | 50 µg | $498 |
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Overview
EV-LuminiteTM: Illuminating the Future of Extracellular Vesicle Research and Analysis
EV-LuminiteTM is an innovative product designed to revolutionize the field of Extracellular Vehicles (EVs) research and analysis. With its advanced technology and unique approach, EV-LuminiteTM enables the precise quantification of subpopulation EV secretion and delivery by harnessing a luminescent signal. This cutting-edge product allows researchers and scientists to shed light on the dynamics and magnitude of EV release, transfer and subpopulation EV function, opening new avenues for understanding cellular communication and therapeutic applications, including studies on the biodistribution of EVs in vivo.
SBI’s EV-LuminiteTM offers:
- Accurate Luminous Quantification
- High Sensitivity and Reliability
- Stable Lenti-based System
- Enhanced Visibility into Subpopulation EV Processes
- Customizable High-Content Screening (HCS) Applications
- Advanced Research and Therapeutic Potential
Unlock the secrets hidden within extracellular vesicles and elevate your research to new heights with EV-LuminiteTM. Join the revolution in cellular communication and explore the limitless possibilities this innovative tool offers. Illuminate the future of EV world with EV-LuminiteTM.
EV-LuminiteTM constructs 
Figure 1: EV-LuminiteTM constructs.Nluc enzyme was cloned into CD9, CD63, or CD81 encoding lenti construct at the N-terminal position with either puromycin or blasticidin resistant. Middle: Scheme of the topology of a tetraspanin (CD9, CD63, or CD81) with N-terminal Nluc-tag.
References
How It Works
EV- LuminteTM works by utilizing Nano luciferase detection system to quantify the production/secretion of Extracellular Vesicles (EVs), evaluate EV delivery and even tracing the cellular communication of EV. Here's an overview of how it works.
Figure 2: Bright up your EV research with EV-LuminiteTM. Scheme of how EV-LuminiteTM can help with your EV research. Transduce your target cells with EV- LuminteTM lentivirus to generate EV-LuminiteTM stable cell line. EV released from EV-LuminteTM stable cell line can be qualified by nano luciferase assay, can be delivered to recipient cells to assess EV delivery/uptaken or can be delivered in mice to study EV dynamic biodistribution. The EV-LuminiteTM stable cell line can be used for any customized high-content screening, such as screening for compound that can increase EV secretion, et al.
Supporting Data
Figure 3: EV derived from EV-LuminiteTM Stable cell lines reveal strong Nano Luciferase activity. EV were isolated from Nluc-CD9 and Nluc-CD81 EV-LuminiteTM Stable cell lines using ExoQuick-TC. Nano luciferase activity was measured using Nano-Glo Luciferase Assay and normalized by EV protein amount.
EV-LuminiteTM offers accurate and sensitive quantification of EV
Figure 4: EV-LuminiteTM allows accurate and sensitive quantification of EV. EV from Nluc-CD63 EV-LuminiteTM stable cell line was isolated using ExoQuick-TC. A) EV samples with different protein amount were used for measuring the Nano luciferase activity by Nano-Glo Luciferase Assay. B) EV samples with series dilution were used for measuring the Nano luciferase activity by Nano-Glo Luciferase Assay and particle number by NTA. Nano luciferase activity shows nice correlation with EV protein amount and particle number.
EV-LuminiteTM enables sensitive measurement of EV delivery to recipient cells
Figure 5: EV-LuminiteTM provides a sensitive means of quantifying the delivery of EV to recipient cells. EV were isolated from Nluc-CD9 and Nluc-CD63 EV-LuminiteTM Stable cell lines using ExoQuick-TC. Isolated EV were then added to the culture medium of HEK 293 cells. 24-48 hours after EV delivery, HEK 293 cells were carefully washed then lysed. Nano luciferase activity of recipient cells were measured using Nano-Glo luciferase assay to assess the EV delivery.
EV-LuminiteTM empowers the evaluation of reagent impact on EV secretion/production
Figure 6: EV-LuminiteTM serves as a useful tool to assess the effect of reagent on EV secretion/production. Nluc-CD9 and Nluc-CD81 EV-LuminiteTM stable cells were treated with DMSO or Bafilomycin A1 (100nM) for over 5 hours. EV were isolated using ExQuick-TC. Nano luciferase activity of isolated EV was measured using Nano-Glo luciferase assay normalized by EV protein amount.
FAQs
Resources
Citations
Related Products
Products
| Catalog Number | Description | Size | Price | Quantity | Add to Cart | |||
|---|---|---|---|---|---|---|---|---|
| EVLT-CD9-PA-P | EV-LuminiteTM pCDH-MSCV-NLuc-CD9-EF1-Puro Expression Lentivector | 10 µg | $789 |
|
||||
| EVLT-CD9-PA-B | EV-LuminiteTM pCDH-MSCV-NLuc-CD9- EF1-Blast Expression Lentivector | 10 µg | $789 |
|
||||
| EVLT-CD63-PA-B | EV-LuminiteTM pCDH-MSCV-NLuc-CD63- EF1-Blast Expression Lentivector | 10 µg | $789 |
|
||||
| EVLT-CD63-PA-P | EV-LuminiteTM pCDH-MSCV-NLuc-CD63- EF1-Puro Expression Lentivector | 10 µg | $789 |
|
||||
| EVLT-CD81-PA-P | EV-LuminiteTM pCDH-MSCV-NLuc-CD81- EF1-Puro Expression Lentivector | 10 µg | $789 |
|
||||
| EVLT-CD81-PA-B | EV-LuminiteTM pCDH-MSCV-NLuc-CD81- EF1-Blast Expression Lentivector | 10 µg | $789 |
|
||||
| EVLT-CD9-VA-P | EV-LuminiteTM pCDH-MSCV-NLuc-CD9-EF1-Puro Expression Lentivirus | >1 x 10^6 IFUs | $789 |
|
||||
| EVLT-CD63-VA-P | EV-LuminiteTM pCDH-MSCV-NLuc-CD63- EF1-Puro Expression Lentivirus | >1 x 10^6 IFUs | $789 |
|
||||
| EVLT-CD81-VA-P | EV-LuminiteTM pCDH-MSCV-NLuc-CD81- EF1-Puro Expression Lentivirus | >1 x 10^6 IFUs | $789 |
|
||||
| EVLT-CD9-CL-1 | EV-LuminiteTM MSCV-NLuc-CD9 HEK293 puro resistant stable cell line | 2 x 10^6 Cells | $1355 |
|
||||
| EVLT-CD63-CL-1 | EV-LuminiteTM MSCV-NLuc-CD63 HEK293 puro resistant stable cell line | 2 x 10^6 Cells | $1355 |
|
||||
| EVLT-CD81-CL-1 | EV-LuminiteTM MSCV-NLuc-CD81 HEK293 puro resistant stable cell line | 2 x 10^6 Cells | $1355 |
|
||||
| EVLT-CD9-EXOP-1 | Exosomes from EV-LuminiteTM pCDH-MSCV-NLuc-CD9 HEK293 puro resistant stable cell line | 50 µg | $498 |
|
||||
| EVLT-CD63-EXOP-1 | Exosomes from EV-LuminiteTM pCDH-MSCV-NLuc-CD63 HEK293 puro resistant stable cell line | 50 µg | $498 |
|
||||
| EVLT-CD81-EXOP-1 | Exosomes from EV-LuminiteTM pCDH-MSCV-NLuc-CD81 HEK293 puro resistant stable cell line | 50 µg | $498 |
|
||||
Overview
EV-LuminiteTM: Illuminating the Future of Extracellular Vesicle Research and Analysis
EV-LuminiteTM is an innovative product designed to revolutionize the field of Extracellular Vehicles (EVs) research and analysis. With its advanced technology and unique approach, EV-LuminiteTM enables the precise quantification of subpopulation EV secretion and delivery by harnessing a luminescent signal. This cutting-edge product allows researchers and scientists to shed light on the dynamics and magnitude of EV release, transfer and subpopulation EV function, opening new avenues for understanding cellular communication and therapeutic applications, including studies on the biodistribution of EVs in vivo.
SBI’s EV-LuminiteTM offers:
- Accurate Luminous Quantification
- High Sensitivity and Reliability
- Stable Lenti-based System
- Enhanced Visibility into Subpopulation EV Processes
- Customizable High-Content Screening (HCS) Applications
- Advanced Research and Therapeutic Potential
Unlock the secrets hidden within extracellular vesicles and elevate your research to new heights with EV-LuminiteTM. Join the revolution in cellular communication and explore the limitless possibilities this innovative tool offers. Illuminate the future of EV world with EV-LuminiteTM.
EV-LuminiteTM constructs
Figure 1: EV-LuminiteTM constructs.Nluc enzyme was cloned into CD9, CD63, or CD81 encoding lenti construct at the N-terminal position with either puromycin or blasticidin resistant. Middle: Scheme of the topology of a tetraspanin (CD9, CD63, or CD81) with N-terminal Nluc-tag.
References
How It Works
EV- LuminteTM works by utilizing Nano luciferase detection system to quantify the production/secretion of Extracellular Vesicles (EVs), evaluate EV delivery and even tracing the cellular communication of EV. Here's an overview of how it works.
Figure 2: Bright up your EV research with EV-LuminiteTM. Scheme of how EV-LuminiteTM can help with your EV research. Transduce your target cells with EV- LuminteTM lentivirus to generate EV-LuminiteTM stable cell line. EV released from EV-LuminteTM stable cell line can be qualified by nano luciferase assay, can be delivered to recipient cells to assess EV delivery/uptaken or can be delivered in mice to study EV dynamic biodistribution. The EV-LuminiteTM stable cell line can be used for any customized high-content screening, such as screening for compound that can increase EV secretion, et al.
Supporting Data
Figure 3: EV derived from EV-LuminiteTM Stable cell lines reveal strong Nano Luciferase activity. EV were isolated from Nluc-CD9 and Nluc-CD81 EV-LuminiteTM Stable cell lines using ExoQuick-TC. Nano luciferase activity was measured using Nano-Glo Luciferase Assay and normalized by EV protein amount.
EV-LuminiteTM offers accurate and sensitive quantification of EV
Figure 4: EV-LuminiteTM allows accurate and sensitive quantification of EV. EV from Nluc-CD63 EV-LuminiteTM stable cell line was isolated using ExoQuick-TC. A) EV samples with different protein amount were used for measuring the Nano luciferase activity by Nano-Glo Luciferase Assay. B) EV samples with series dilution were used for measuring the Nano luciferase activity by Nano-Glo Luciferase Assay and particle number by NTA. Nano luciferase activity shows nice correlation with EV protein amount and particle number.
EV-LuminiteTM enables sensitive measurement of EV delivery to recipient cells
Figure 5: EV-LuminiteTM provides a sensitive means of quantifying the delivery of EV to recipient cells. EV were isolated from Nluc-CD9 and Nluc-CD63 EV-LuminiteTM Stable cell lines using ExoQuick-TC. Isolated EV were then added to the culture medium of HEK 293 cells. 24-48 hours after EV delivery, HEK 293 cells were carefully washed then lysed. Nano luciferase activity of recipient cells were measured using Nano-Glo luciferase assay to assess the EV delivery.
EV-LuminiteTM empowers the evaluation of reagent impact on EV secretion/production
Figure 6: EV-LuminiteTM serves as a useful tool to assess the effect of reagent on EV secretion/production. Nluc-CD9 and Nluc-CD81 EV-LuminiteTM stable cells were treated with DMSO or Bafilomycin A1 (100nM) for over 5 hours. EV were isolated using ExQuick-TC. Nano luciferase activity of isolated EV was measured using Nano-Glo luciferase assay normalized by EV protein amount.