Exo-Fect™ Exosome Transfection Kit
- Easy-to-use with a fast and straightforward loading protocol
- Introduces a wide range of biomolecules directly into isolated exosomes:
- RNAs, including siRNAs, miRNAs, and mRNAs
- DNAs, including plasmids
- Metabolites and other small molecules
Products
Catalog Number | Description | Size | Price | Quantity | Add to Cart | |||
---|---|---|---|---|---|---|---|---|
EXFT10A-1 | Exo-Fect Exosome Transfection Kit | 10 Reactions | $247 |
|
||||
EXFT20A-1 | Exo-Fect Exosome Transfection Kit | 20 Reactions | $445 |
|
Overview
Overview
Putting exosomes to work: Load your cargo directly into isolated exosomes With Exo-Fect, you can turn isolated exosomes into cargo delivery vehicles that introduce RNAs, DNAs (including plasmids), and even small molecules into recipient cells. Simply combine isolated exosomes with Exo-Fect and your desired cargo, and in less than an hour your loaded exosomes will be ready to add to recipient cells.- Easy-to-use with a fast and straightforward loading protocol
- Introduces a wide range of biomolecules directly into isolated exosomes:
- RNAs, including siRNAs, miRNAs, and mRNAs
- DNAs, including plasmids
- Metabolites and other small molecules
- Enables non-viral transduction and stable cell line creation
- Provides an alternative gene delivery method for hard-to-transfect cells
References
How It Works
How It Works
Quickly and easily load cargo into isolated exosomes
Simply combine the isolated exosomes with Exo-Fect Reagent and cargo, perform two incubations for a total of 40 minutes, and then pellet your exosomes. The Kit comes with all of the reagents you need to load cargo into exosomes and concentrate them for delivery to target cells. The protocol takes less than an hour and is highly efficient at loading cargo into exosomes for transport and delivery.
Exo-Fect is also compatible with the EV-Entry Reagent, which maximizes cargo uptake by recipient cells.
Supporting Data
Supporting Data
See how Exo-Fect can be used to create cargo-delivering exosomes
Exo-Fect efficiently loads small RNAs into exosomes for delivery to recipient cells
Exo-Fect efficiently loads mRNAs and DNA plasmids into exosomes for delivery to recipient cells
Exo-Fect efficiently loads small molecules into exosomes for delivery to recipient cells
FAQs
Related Products
Resources
Citations
-
Kim, JY, et al. (2024) Combinatory Nanovesicle with siRNA-Loaded Extracellular Vesicle and IGF-1 for Osteoarthritis Treatments. International journal of molecular sciences. 2024; 25(10). PM ID: 38791285
-
Heo, J & Ryu, J. (2024) Exosomal noncoding RNA: a potential therapy for retinal vascular diseases. Molecular Therapy - Nucleic Acids. 2024;:102128. Link: Molecular Therapy - Nucleic Acids
-
Zhang, L, et al. (2024) Engineering M2 type macrophage-derived exosomes for autoimmune hepatitis immunotherapy via loading siRIPK3. Biomedicine & pharmacotherapy = Biomedecine & pharmacotherapie. 2024; 171:116161. PM ID: 38244330
-
Xu, K, et al. (2024) Elevated extracellular matrix protein 1 in circulating extracellular vesicles supports breast cancer progression under obesity conditions. Nature communications. 2024; 15(1):1685. PM ID: 38402239
-
Guan, T, et al. (2024) Oxidized SOD1 accelerates cellular senescence in neural stem cells. Stem cell research & therapy. 2024; 15(1):55. PM ID: 38414053
-
Mao, Y, et al. (2024) Hypoxia induced exosomal Circ-ZNF609 promotes pre-metastatic niche formation and cancer progression via miR-150-5p/VEGFA and HuR/ZO-1 axes in esophageal squamous cell carcinoma. Cell death discovery. 2024; 10(1):133. PM ID: 38472174
-
Zhang, Y, et al. (2024) Islet-resident macrophage-derived miR-155 promotes β cell decompensation via targeting PDX1. iScience. 2024; 27(4):109540. Link: iScience
-
Chen, JG, et al. (2024) Engineered hsa-miR-455-3p-Abundant Extracellular Vesicles Derived from 3D-Cultured Adipose Mesenchymal Stem Cells for Tissue-Engineering Hyaline Cartilage Regeneration. Advanced healthcare materials. 2024;:e2304194. PM ID: 38508211
-
Jiang, P, et al. (2024) RVG29 Peptide-Modified Exosomes Loaded with Mir-133b Mediate the RhoA-ROCK Pathway to Improve Motor and Neurological Symptoms in Parkinson’s Disease. ACS biomaterials science & engineering. 2024;. PM ID: 38578110
-
Sun, H, et al. (2024) C166 EVs potentiate miR cardiac reprogramming via miR-148a-3p. Journal of molecular and cellular cardiology. 2024; 190:48-61. PM ID: 38582260
-
Bader, J, et al. (2024) Loading of extracellular vesicles with nucleic acids via hybridization with sponge-like lipid nanoparticles. bioRxiv. 2024;. Link: bioRxiv
-
Menjivar, NG, et al. (2024) MicroRNA Nano-Shuttles: Engineering Extracellular Vesicles as a Cutting-Edge Biotechnology Platform for Clinical Use in Therapeutics. Biological procedures online. 2024; 26(1):14. PM ID: 38773366
-
Xie, W, et al. (2023) Inhibition of osteosarcoma by European Mistletoe derived val-miR218. Extracellular Vesicles and Circulating Nucleic Acids. 2023; 4(2):306-322. Link: Extracellular Vesicles and Circulating Nucleic Acids
-
Lang, HL, et al. (2023) Small extracellular vesicles secreted by induced pluripotent stem cell-derived mesenchymal stem cells improve postoperative cognitive dysfunction in mice with diabetes. Neural regeneration research. 2023; 18(3):609-617. PM ID: 36018185
-
Lin, W, et al. (2023) Tumor-intrinsic YTHDF1 drives immune evasion and resistance to immune checkpoint inhibitors via promoting MHC-I degradation. Nature communications. 2023; 14(1):265. PM ID: 36650153
-
Zhao, W, et al. (2023) Accelerating corneal wound healing using exosome-mediated targeting of NF-κB c-Rel. Inflammation and regeneration. 2023; 43(1):6. PM ID: 36703231
-
Lu, Y, et al. (2023) Exosome-Based Carrier for RNA Delivery: Progress and Challenges. Pharmaceutics. 2023; 15(2). PM ID: 36839920
-
Ellipilli, S, et al. (2023) Ligand-displaying-exosomes using RNA nanotechnology for targeted delivery of multi-specific drugs for liver cancer regression. Nanomedicine : nanotechnology, biology, and medicine. 2023; 50:102667. PM ID: 36948369
-
Zheng, Z, et al. (2023) Acinous cell AR42J-derived exosome miR125b-5p promotes acute pancreatitis exacerbation by inhibiting M2 macrophage polarization via PI3K/AKT signaling pathway. World Journal of Gastrointestinal Surgery. 2023; 15(4):600-620. Link: World Journal of Gastrointestinal Surgery
-
Hao, L, et al. (2023) Bone targeting miR-26a loaded exosome-mimetics for bone regeneration therapy by activating Wnt signaling pathway. Chemical Engineering Journal. 2023; 471:144594. Link: Chemical Engineering Journal
- See More
Products
Catalog Number | Description | Size | Price | Quantity | Add to Cart | |||
---|---|---|---|---|---|---|---|---|
EXFT10A-1 | Exo-Fect Exosome Transfection Kit | 10 Reactions | $247 |
|
||||
EXFT20A-1 | Exo-Fect Exosome Transfection Kit | 20 Reactions | $445 |
|
Overview
Overview
Putting exosomes to work: Load your cargo directly into isolated exosomes With Exo-Fect, you can turn isolated exosomes into cargo delivery vehicles that introduce RNAs, DNAs (including plasmids), and even small molecules into recipient cells. Simply combine isolated exosomes with Exo-Fect and your desired cargo, and in less than an hour your loaded exosomes will be ready to add to recipient cells.- Easy-to-use with a fast and straightforward loading protocol
- Introduces a wide range of biomolecules directly into isolated exosomes:
- RNAs, including siRNAs, miRNAs, and mRNAs
- DNAs, including plasmids
- Metabolites and other small molecules
- Enables non-viral transduction and stable cell line creation
- Provides an alternative gene delivery method for hard-to-transfect cells
References
How It Works
How It Works
Quickly and easily load cargo into isolated exosomes
Simply combine the isolated exosomes with Exo-Fect Reagent and cargo, perform two incubations for a total of 40 minutes, and then pellet your exosomes. The Kit comes with all of the reagents you need to load cargo into exosomes and concentrate them for delivery to target cells. The protocol takes less than an hour and is highly efficient at loading cargo into exosomes for transport and delivery.
Exo-Fect is also compatible with the EV-Entry Reagent, which maximizes cargo uptake by recipient cells.
Supporting Data
Supporting Data
See how Exo-Fect can be used to create cargo-delivering exosomes
Exo-Fect efficiently loads small RNAs into exosomes for delivery to recipient cells
Exo-Fect efficiently loads mRNAs and DNA plasmids into exosomes for delivery to recipient cells
Exo-Fect efficiently loads small molecules into exosomes for delivery to recipient cells
FAQs
Citations
-
Kim, JY, et al. (2024) Combinatory Nanovesicle with siRNA-Loaded Extracellular Vesicle and IGF-1 for Osteoarthritis Treatments. International journal of molecular sciences. 2024; 25(10). PM ID: 38791285
-
Heo, J & Ryu, J. (2024) Exosomal noncoding RNA: a potential therapy for retinal vascular diseases. Molecular Therapy - Nucleic Acids. 2024;:102128. Link: Molecular Therapy - Nucleic Acids
-
Zhang, L, et al. (2024) Engineering M2 type macrophage-derived exosomes for autoimmune hepatitis immunotherapy via loading siRIPK3. Biomedicine & pharmacotherapy = Biomedecine & pharmacotherapie. 2024; 171:116161. PM ID: 38244330
-
Xu, K, et al. (2024) Elevated extracellular matrix protein 1 in circulating extracellular vesicles supports breast cancer progression under obesity conditions. Nature communications. 2024; 15(1):1685. PM ID: 38402239
-
Guan, T, et al. (2024) Oxidized SOD1 accelerates cellular senescence in neural stem cells. Stem cell research & therapy. 2024; 15(1):55. PM ID: 38414053
-
Mao, Y, et al. (2024) Hypoxia induced exosomal Circ-ZNF609 promotes pre-metastatic niche formation and cancer progression via miR-150-5p/VEGFA and HuR/ZO-1 axes in esophageal squamous cell carcinoma. Cell death discovery. 2024; 10(1):133. PM ID: 38472174
-
Zhang, Y, et al. (2024) Islet-resident macrophage-derived miR-155 promotes β cell decompensation via targeting PDX1. iScience. 2024; 27(4):109540. Link: iScience
-
Chen, JG, et al. (2024) Engineered hsa-miR-455-3p-Abundant Extracellular Vesicles Derived from 3D-Cultured Adipose Mesenchymal Stem Cells for Tissue-Engineering Hyaline Cartilage Regeneration. Advanced healthcare materials. 2024;:e2304194. PM ID: 38508211
-
Jiang, P, et al. (2024) RVG29 Peptide-Modified Exosomes Loaded with Mir-133b Mediate the RhoA-ROCK Pathway to Improve Motor and Neurological Symptoms in Parkinson’s Disease. ACS biomaterials science & engineering. 2024;. PM ID: 38578110
-
Sun, H, et al. (2024) C166 EVs potentiate miR cardiac reprogramming via miR-148a-3p. Journal of molecular and cellular cardiology. 2024; 190:48-61. PM ID: 38582260
-
Bader, J, et al. (2024) Loading of extracellular vesicles with nucleic acids via hybridization with sponge-like lipid nanoparticles. bioRxiv. 2024;. Link: bioRxiv
-
Menjivar, NG, et al. (2024) MicroRNA Nano-Shuttles: Engineering Extracellular Vesicles as a Cutting-Edge Biotechnology Platform for Clinical Use in Therapeutics. Biological procedures online. 2024; 26(1):14. PM ID: 38773366
-
Xie, W, et al. (2023) Inhibition of osteosarcoma by European Mistletoe derived val-miR218. Extracellular Vesicles and Circulating Nucleic Acids. 2023; 4(2):306-322. Link: Extracellular Vesicles and Circulating Nucleic Acids
-
Lang, HL, et al. (2023) Small extracellular vesicles secreted by induced pluripotent stem cell-derived mesenchymal stem cells improve postoperative cognitive dysfunction in mice with diabetes. Neural regeneration research. 2023; 18(3):609-617. PM ID: 36018185
-
Lin, W, et al. (2023) Tumor-intrinsic YTHDF1 drives immune evasion and resistance to immune checkpoint inhibitors via promoting MHC-I degradation. Nature communications. 2023; 14(1):265. PM ID: 36650153
-
Zhao, W, et al. (2023) Accelerating corneal wound healing using exosome-mediated targeting of NF-κB c-Rel. Inflammation and regeneration. 2023; 43(1):6. PM ID: 36703231
-
Lu, Y, et al. (2023) Exosome-Based Carrier for RNA Delivery: Progress and Challenges. Pharmaceutics. 2023; 15(2). PM ID: 36839920
-
Ellipilli, S, et al. (2023) Ligand-displaying-exosomes using RNA nanotechnology for targeted delivery of multi-specific drugs for liver cancer regression. Nanomedicine : nanotechnology, biology, and medicine. 2023; 50:102667. PM ID: 36948369
-
Zheng, Z, et al. (2023) Acinous cell AR42J-derived exosome miR125b-5p promotes acute pancreatitis exacerbation by inhibiting M2 macrophage polarization via PI3K/AKT signaling pathway. World Journal of Gastrointestinal Surgery. 2023; 15(4):600-620. Link: World Journal of Gastrointestinal Surgery
-
Hao, L, et al. (2023) Bone targeting miR-26a loaded exosome-mimetics for bone regeneration therapy by activating Wnt signaling pathway. Chemical Engineering Journal. 2023; 471:144594. Link: Chemical Engineering Journal
- See More