Exo-Fect™ siRNA/miRNA Transfection Kit
- Efficient delivery—siRNA/miRNA-loaded EVs deliver cargo to as many as 95% of target cells*
- Validated efficacy—siRNA-loaded EVs lead to significant knockdown (>80%) of target gene expression in transfected cells**
- No toxicity—unlike commonly-used lipid-based transfection reagents, the Exo-Fect siRNA/miRNA Transfection Reagent causes no toxicity in tested cells
- Fast, easy workflow—go from prepared small RNA to ready-to-use, RNA-loaded EVs in less than 1.5-hours and with only 15-minutes of hands-on time
- Minimal background—a clean-up step ensures complete removal of free small RNA and excess transfection reagent for clean, ready-to-use, RNA-loaded EVs
Products
Catalog Number | Description | Size | Price | Quantity | Add to Cart | |||
---|---|---|---|---|---|---|---|---|
EXFT200A-1 | Exo-Fect™ siRNA/miRNA Transfection Kit | 20 Reactions | $427 |
|
Overview
Overview
Headache-free loading of siRNAs and miRNAs into extracellular vesiclesWhether you’re engineering extracellular vesicles (EVs) as part of therapeutics development1 or using them as a tool for functional studies of EV-mediated nucleic acid delivery2, getting the desired miRNA or siRNA cargo into EVs is a critical first step. SBI helps you efficiently and effortlessly get this step done with the Exo-Fect™ siRNA/miRNA Transfection Kit, a first-in-class reagent specifically designed for loading small RNAs into EVs.
Unlike electroporation, a common method for introducing small RNAs into EVs3, the Exo-Fect workflow does not require access to specialized equipment or the same time-consuming optimization of voltage and timing. Instead, it leverages our proprietary Cell-Penetrating Peptide (CPP) technology to transfer siRNAs or miRNAs to the inside of your EVs.
This next-generation EV transfection reagent offers the following benefits:
- Efficient delivery—siRNA/miRNA-loaded EVs deliver cargo to as many as 95% of target cells*
- Validated efficacy—siRNA-loaded EVs lead to significant knockdown (>80%) of target gene expression in transfected cells**
- No toxicity—unlike commonly-used lipid-based transfection reagents, the Exo-Fect siRNA/miRNA Transfection Reagent causes no toxicity in tested cells
- Fast, easy workflow—go from prepared small RNA to ready-to-use, RNA-loaded EVs in less than 1.5-hours and with only 15-minutes of hands-on time
- Minimal background—a clean-up step ensures complete removal of free small RNA and excess transfection reagent for clean, ready-to-use, RNA-loaded EVs
*As assessed by exposing non-fluorescent target cells to EVs loaded with Cy3-labeled control siRNA using Exo-Fect siRNA/miRNA transfection reagent, and then measuring the percentage of fluorescent cells (HeLa, HUVEC, and HEK293 cells were all tested).
** As assessed using qPCR analysis of HPRT gene expression in cells treated with either HPRT-targeting siRNA or control siRNA.
References- Chen Z, et al. Therapeutic Potential of Mesenchymal Cell-Derived miRNA-150-5p-Expressing Exosomes in Rheumatoid Arthritis Mediated by the Modulation of MMP14 and VEGF. J Immunol. 2018 Oct 15;201(8):2472-2482. PMCID: PMC6176104.
- Pegtel DM, et al. Functional delivery of viral miRNAs via exosomes. Proc Natl Acad Sci U S A. 2010;107(14):6328-33. PMCID: PMC2851954.
- Alvarez-Erviti, L et al. Delivery of siRNA to the mouse brain by systemic injection of targeted exosomes. Nat Biotechnol. 2011 Apr; 29:341–345. PMID: 21423189.
References
How It Works
How It Works
Quick and easy loading of siRNA or miRNA into EVsThe Exo-Fect siRNA/miRNA Transfection Kit workflow is quick and easy—complete in 1.5 hours and with only 15-minutes of hands-on time.
The workflow consists of two steps:
- Load your siRNA or miRNA into already-isolated EVs.
- Incubate siRNA or miRNA with Exo-Fect siRNA/miRNA Transfection reagent for 15-minutes at room temperature.
- Add isolated EVs to the mixture and incubate at 37°C for 1-hour.
- Clean-up the reaction by removing free siRNA/miRNA, transfection reagent, and free siRNA/miRNA-Exo-Fect complexes
- Transfer reaction to the included, pre-washed spin-column and incubate with gentle rotation for 10-minutes at room temperature.
- Collect EVs by centrifuging the spin-column for 30-seconds at 1,000 x g. Loaded EVs are ready for use in downstream applications.
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Products
Catalog Number | Description | Size | Price | Quantity | Add to Cart | |||
---|---|---|---|---|---|---|---|---|
EXFT200A-1 | Exo-Fect™ siRNA/miRNA Transfection Kit | 20 Reactions | $427 |
|
Overview
Overview
Headache-free loading of siRNAs and miRNAs into extracellular vesiclesWhether you’re engineering extracellular vesicles (EVs) as part of therapeutics development1 or using them as a tool for functional studies of EV-mediated nucleic acid delivery2, getting the desired miRNA or siRNA cargo into EVs is a critical first step. SBI helps you efficiently and effortlessly get this step done with the Exo-Fect™ siRNA/miRNA Transfection Kit, a first-in-class reagent specifically designed for loading small RNAs into EVs.
Unlike electroporation, a common method for introducing small RNAs into EVs3, the Exo-Fect workflow does not require access to specialized equipment or the same time-consuming optimization of voltage and timing. Instead, it leverages our proprietary Cell-Penetrating Peptide (CPP) technology to transfer siRNAs or miRNAs to the inside of your EVs.
This next-generation EV transfection reagent offers the following benefits:
- Efficient delivery—siRNA/miRNA-loaded EVs deliver cargo to as many as 95% of target cells*
- Validated efficacy—siRNA-loaded EVs lead to significant knockdown (>80%) of target gene expression in transfected cells**
- No toxicity—unlike commonly-used lipid-based transfection reagents, the Exo-Fect siRNA/miRNA Transfection Reagent causes no toxicity in tested cells
- Fast, easy workflow—go from prepared small RNA to ready-to-use, RNA-loaded EVs in less than 1.5-hours and with only 15-minutes of hands-on time
- Minimal background—a clean-up step ensures complete removal of free small RNA and excess transfection reagent for clean, ready-to-use, RNA-loaded EVs
*As assessed by exposing non-fluorescent target cells to EVs loaded with Cy3-labeled control siRNA using Exo-Fect siRNA/miRNA transfection reagent, and then measuring the percentage of fluorescent cells (HeLa, HUVEC, and HEK293 cells were all tested).
** As assessed using qPCR analysis of HPRT gene expression in cells treated with either HPRT-targeting siRNA or control siRNA.
References- Chen Z, et al. Therapeutic Potential of Mesenchymal Cell-Derived miRNA-150-5p-Expressing Exosomes in Rheumatoid Arthritis Mediated by the Modulation of MMP14 and VEGF. J Immunol. 2018 Oct 15;201(8):2472-2482. PMCID: PMC6176104.
- Pegtel DM, et al. Functional delivery of viral miRNAs via exosomes. Proc Natl Acad Sci U S A. 2010;107(14):6328-33. PMCID: PMC2851954.
- Alvarez-Erviti, L et al. Delivery of siRNA to the mouse brain by systemic injection of targeted exosomes. Nat Biotechnol. 2011 Apr; 29:341–345. PMID: 21423189.
References
How It Works
How It Works
Quick and easy loading of siRNA or miRNA into EVsThe Exo-Fect siRNA/miRNA Transfection Kit workflow is quick and easy—complete in 1.5 hours and with only 15-minutes of hands-on time.
The workflow consists of two steps:
- Load your siRNA or miRNA into already-isolated EVs.
- Incubate siRNA or miRNA with Exo-Fect siRNA/miRNA Transfection reagent for 15-minutes at room temperature.
- Add isolated EVs to the mixture and incubate at 37°C for 1-hour.
- Clean-up the reaction by removing free siRNA/miRNA, transfection reagent, and free siRNA/miRNA-Exo-Fect complexes
- Transfer reaction to the included, pre-washed spin-column and incubate with gentle rotation for 10-minutes at room temperature.
- Collect EVs by centrifuging the spin-column for 30-seconds at 1,000 x g. Loaded EVs are ready for use in downstream applications.