ExoELISA-ULTRA Complete Kit (GroEL, For E. coli OMV Detection)
- Sensitive - detect as little as 0.5 µg protein equivalent
- Fast - complete in less than 4-hours; no more overnight incubation
- Flexible - compatible with common OMV isolation methods (e.g. ExoBacteriaTM OMV Isolation Kit, ultracentrifugation) from E.coli culture
- Quantitative - calibrated internal standards enable quantitation of OMVs carrying GroEL
- Sample-saving - requires significantly low sample amount, leaving more for other downstream applications
Products
| Catalog Number | Description | Size | Price | Quantity | Add to Cart | |||
|---|---|---|---|---|---|---|---|---|
| EXEL-ULTRA-GroEL-1 | ExoELISA-ULTRA Complete Kit (GroEL, For E.coli OMV Detection) | 96 Reactions | $699 |
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Overview
Completing the collection of our popular ExoELISA-ULTRA Kits, the ExoELISA-ULTRA GroEL Kit is brought to users as the first and only kit for easy quantitation and characterization of OMVs from E. coli, with supreme sensitivity of OMV detection - as low as 0.5 µg protein equivalent - and a total assay time of only 4 hours.
Configured for detection of E.coli OMVs, an OMV associated molecular chaperonin GroEL was selected as the bacterial OMV marker 1-4. The ExoELISA-ULTRA GroEL detection assay is based on an ultra-sensitive direct capture colorimetric ELISA that is compatible with OMVs isolated from E. coli culture by common OMV isolation methods. To enable quantitation of OMVs carrying GroEL in your target sample, the kit includes an internal standard calibrated to OMVs isolated with different OMV isolation methods that have been analyzed by nanoparticle tracking analysis (NTA). This assay is also predicted to work with OMVs derived from Shigella dysenteriae and Salmonella enterica.
One ExoELISA-ULTRA GroEL Kit contains all of the necessary reagents (including assay plate) to perform up to 96 reactions.
- Sensitive - detect as little as 0.5 µg protein equivalent
- Fast - complete in less than 4-hours; no more overnight incubation
- Flexible - compatible with common OMV isolation methods (e.g. ExoBacteria™ OMV Isolation Kit, ultracentrifugation) from E.coli culture
- Quantitative - calibrated internal standards enable quantitation of OMVs carrying GroEL
- Sample-saving - requires significantly low sample amount, leaving more for other downstream applications
References
- Alzahrani, H. et al. Characterisation of outer membrane vesicles from a neonatal meningitic strain of Cronobacter sakazakii. FEMS Microbiol Lett. 2015 Jun; 362(12):fnv085.
- Cao, S. et al. Secretome and Comparative Proteomics of Yersinia pestis Identify Two Novel E3 Ubiquitin Ligases That Contribute to Plague Virulence. Molecular & Cellular Proteomics. 2021; 20:100066. doi: 10.1016/j.mcpro.2021.100066.
- Tiwari, V. et al. Significances of OMV and Extracellular Vesicle Proteomics. Journal of Data Mining in Genomics & Proteomics. 2017; 08. 10.4172/2153-0602.1000208.
- Zhu, D. et al. (2023). Characterization of Molecular Chaperone GroEL as a Potential Virulence Factor in Cronobacter sakazakii. Foods. 2023 Sep 12;12(18):3404.
Associated Kits
Choose the exosome quantitation method that’s best for your studies
| ExoELISA-ULTRA Complete Kits | EXOCET | FluoroCet | |
|---|---|---|---|
| Use | For fast and sensitive antibody-based quantitation of exosomes | For fast quantitation of extracellular vesicles with moderate sample input requirements | For the most sensitive quantitation of extracellular vesicles with very low sample input requirements |
| Detection method | Antibody | Enzymatic | Enzymatic |
| Quantitation chemistry | Enzymatic (HRP) | Colorimetric | Fluorescent |
| Total protocol time | 4 hours (no overnight incubation) | 20 min | 60 min |
| Input sample amount (protein equivalent) | 1 – 200 µg | 50 µg | <1 µg |
| Learn More | ExoELISA-ULTRA CD63 ExoELISA-ULTRA CD81 ExoELISA-ULTRA CD9 | EXOCET | FluoroCet |
References
How It Works
Supporting Data
Figure 1.ExoELISA-ULTRA GroEL standard curve shows robust linearity down to ~ 0.82 x 109 OMVs. OD 450 nm values of OMVs isolated with common OMV isolation methods (Ultracentrifuge and ExoBacteria™ OMV Isolation Kit) fall well within the standard curve for the assay.
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Related Products
Products
| Catalog Number | Description | Size | Price | Quantity | Add to Cart | |||
|---|---|---|---|---|---|---|---|---|
| EXEL-ULTRA-GroEL-1 | ExoELISA-ULTRA Complete Kit (GroEL, For E.coli OMV Detection) | 96 Reactions | $699 |
|
||||
Overview
Completing the collection of our popular ExoELISA-ULTRA Kits, the ExoELISA-ULTRA GroEL Kit is brought to users as the first and only kit for easy quantitation and characterization of OMVs from E. coli, with supreme sensitivity of OMV detection - as low as 0.5 µg protein equivalent - and a total assay time of only 4 hours.
Configured for detection of E.coli OMVs, an OMV associated molecular chaperonin GroEL was selected as the bacterial OMV marker 1-4. The ExoELISA-ULTRA GroEL detection assay is based on an ultra-sensitive direct capture colorimetric ELISA that is compatible with OMVs isolated from E. coli culture by common OMV isolation methods. To enable quantitation of OMVs carrying GroEL in your target sample, the kit includes an internal standard calibrated to OMVs isolated with different OMV isolation methods that have been analyzed by nanoparticle tracking analysis (NTA). This assay is also predicted to work with OMVs derived from Shigella dysenteriae and Salmonella enterica.
One ExoELISA-ULTRA GroEL Kit contains all of the necessary reagents (including assay plate) to perform up to 96 reactions.
- Sensitive - detect as little as 0.5 µg protein equivalent
- Fast - complete in less than 4-hours; no more overnight incubation
- Flexible - compatible with common OMV isolation methods (e.g. ExoBacteria™ OMV Isolation Kit, ultracentrifugation) from E.coli culture
- Quantitative - calibrated internal standards enable quantitation of OMVs carrying GroEL
- Sample-saving - requires significantly low sample amount, leaving more for other downstream applications
References
- Alzahrani, H. et al. Characterisation of outer membrane vesicles from a neonatal meningitic strain of Cronobacter sakazakii. FEMS Microbiol Lett. 2015 Jun; 362(12):fnv085.
- Cao, S. et al. Secretome and Comparative Proteomics of Yersinia pestis Identify Two Novel E3 Ubiquitin Ligases That Contribute to Plague Virulence. Molecular & Cellular Proteomics. 2021; 20:100066. doi: 10.1016/j.mcpro.2021.100066.
- Tiwari, V. et al. Significances of OMV and Extracellular Vesicle Proteomics. Journal of Data Mining in Genomics & Proteomics. 2017; 08. 10.4172/2153-0602.1000208.
- Zhu, D. et al. (2023). Characterization of Molecular Chaperone GroEL as a Potential Virulence Factor in Cronobacter sakazakii. Foods. 2023 Sep 12;12(18):3404.
Associated Kits
Choose the exosome quantitation method that’s best for your studies
| ExoELISA-ULTRA Complete Kits | EXOCET | FluoroCet | |
|---|---|---|---|
| Use | For fast and sensitive antibody-based quantitation of exosomes | For fast quantitation of extracellular vesicles with moderate sample input requirements | For the most sensitive quantitation of extracellular vesicles with very low sample input requirements |
| Detection method | Antibody | Enzymatic | Enzymatic |
| Quantitation chemistry | Enzymatic (HRP) | Colorimetric | Fluorescent |
| Total protocol time | 4 hours (no overnight incubation) | 20 min | 60 min |
| Input sample amount (protein equivalent) | 1 – 200 µg | 50 µg | <1 µg |
| Learn More | ExoELISA-ULTRA CD63 ExoELISA-ULTRA CD81 ExoELISA-ULTRA CD9 | EXOCET | FluoroCet |
References
How It Works
Supporting Data
Figure 1.ExoELISA-ULTRA GroEL standard curve shows robust linearity down to ~ 0.82 x 109 OMVs. OD 450 nm values of OMVs isolated with common OMV isolation methods (Ultracentrifuge and ExoBacteria™ OMV Isolation Kit) fall well within the standard curve for the assay.