ExoMAX™ Opti Enhancer
- High purity—supports separation of exosomes from viruses and protein aggregates
- High yield—delivers more exosomes than the traditional protocol, start with smaller samples
- More hands-free—three simple steps before the density gradient
- Flexible—compatible with downstream biomarker discovery and functional assays
- Scalable—pellet exosomes from any volume of conditioned media or biofluid and resuspend as needed for the density gradient
Products
Catalog Number | Description | Size | Price | Quantity | Add to Cart | |||
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EXOMAX12A-1 | ExoMAX™ Opti Enhancer | 12 Reactions | $403 |
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EXOMAX24A-1 | ExoMAX™ Opti Enhancer | 1 Kit | $606 |
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Overview
Overview
Easier preparation for density gradient ultracentrifugation
For researchers needing highly pure exosomes, sucrose or OptiPrep™ (iodixanol) density gradient ultracentrifugation are the methods of choice. However, sample preparation prior to the density gradient is a time-consuming and multi-step process. To streamline the pre-density gradient steps, SBI has developed ExoMAX™ Opti Enhancer, an easy-to-use reagent that can move samples to the density gradient in three easy steps.
- High purity—supports separation of exosomes from viruses and protein aggregates
- High yield—delivers more exosomes than the traditional protocol, start with smaller samples
- More hands-free—three simple steps before the density gradient
- Flexible—compatible with downstream biomarker discovery and functional assays
- Scalable—pellet exosomes from any volume of conditioned media or biofluid and resuspend as needed for the density gradient
"We have tested the ExoMAX reagent. It gave us 5-fold more yield than conventional ultracentrifugation in a side-by-side comparison." —Zongdi Feng, Nationwide Children's Hospital
References
How It Works
How It Works
Get to the gradient fast
Instead of multiple low speed centrifugation steps followed by a high-speed ultracentrifugation step (left panel), with ExoMAX Opti Enhancer you can simply centrifuge the cell culture medium or biofluid to pellet cellular debris, incubate with ExoMAX Opti Enhancer, centrifuge again, and load the resuspended pellet onto the density gradient—no preliminary high-speed spin necessary (right panel). The resulting exosomes harvested from the density gradient are present in higher amounts compared to standard preparation methods, allowing you to start with smaller sample volumes, and can be easily separated from other co-precipitating particles such as viruses and protein.
Supporting Data
Supporting Data
High yield exosome isolation from virus-infected cells
To demonstrate the excellent performance of ExoMAX Opti Enhancer, we isolated exosomes from HIV-infected T-cells. Five mL of conditioned medium (for ExoMAX protocol) and 10 mL of conditioned medium (for standard UC protocol) from infected cells were collected, and vesicles isolated using either ExoMAX Opti Enhancer reagent (Figures 1 and 2, top panels) or the standard protocol (Figures 1 and 2, bottom panels) before separation using OptiPrep gradient medium and ultracentrifugation at 110,000g for 70 minutes.
Fractions from the gradient were collected and lysed for subsequent Western blot analysis, which show exosome yield (Figure 1), and separation of exosomes from virus particles (Figure 2). Compared to the standard density ultracentrifugation workflow, ExoMAX Opti Enhancer delivers a higher yield of exosomes (Figure 3).
References
- Narayanan A, et al. Exosomes derived from HIV-1-infected cells contain trans-activation response element RNA. J Biol Chem. 2013; 288(27):20014-33. PMCID: PMC3707700.
FAQs
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Citations
Related Products
Products
Catalog Number | Description | Size | Price | Quantity | Add to Cart | |||
---|---|---|---|---|---|---|---|---|
EXOMAX12A-1 | ExoMAX™ Opti Enhancer | 12 Reactions | $403 |
|
||||
EXOMAX24A-1 | ExoMAX™ Opti Enhancer | 1 Kit | $606 |
|
Overview
Overview
Easier preparation for density gradient ultracentrifugation
For researchers needing highly pure exosomes, sucrose or OptiPrep™ (iodixanol) density gradient ultracentrifugation are the methods of choice. However, sample preparation prior to the density gradient is a time-consuming and multi-step process. To streamline the pre-density gradient steps, SBI has developed ExoMAX™ Opti Enhancer, an easy-to-use reagent that can move samples to the density gradient in three easy steps.
- High purity—supports separation of exosomes from viruses and protein aggregates
- High yield—delivers more exosomes than the traditional protocol, start with smaller samples
- More hands-free—three simple steps before the density gradient
- Flexible—compatible with downstream biomarker discovery and functional assays
- Scalable—pellet exosomes from any volume of conditioned media or biofluid and resuspend as needed for the density gradient
"We have tested the ExoMAX reagent. It gave us 5-fold more yield than conventional ultracentrifugation in a side-by-side comparison." —Zongdi Feng, Nationwide Children's Hospital
References
How It Works
How It Works
Get to the gradient fast
Instead of multiple low speed centrifugation steps followed by a high-speed ultracentrifugation step (left panel), with ExoMAX Opti Enhancer you can simply centrifuge the cell culture medium or biofluid to pellet cellular debris, incubate with ExoMAX Opti Enhancer, centrifuge again, and load the resuspended pellet onto the density gradient—no preliminary high-speed spin necessary (right panel). The resulting exosomes harvested from the density gradient are present in higher amounts compared to standard preparation methods, allowing you to start with smaller sample volumes, and can be easily separated from other co-precipitating particles such as viruses and protein.
Supporting Data
Supporting Data
High yield exosome isolation from virus-infected cells
To demonstrate the excellent performance of ExoMAX Opti Enhancer, we isolated exosomes from HIV-infected T-cells. Five mL of conditioned medium (for ExoMAX protocol) and 10 mL of conditioned medium (for standard UC protocol) from infected cells were collected, and vesicles isolated using either ExoMAX Opti Enhancer reagent (Figures 1 and 2, top panels) or the standard protocol (Figures 1 and 2, bottom panels) before separation using OptiPrep gradient medium and ultracentrifugation at 110,000g for 70 minutes.
Fractions from the gradient were collected and lysed for subsequent Western blot analysis, which show exosome yield (Figure 1), and separation of exosomes from virus particles (Figure 2). Compared to the standard density ultracentrifugation workflow, ExoMAX Opti Enhancer delivers a higher yield of exosomes (Figure 3).
References
- Narayanan A, et al. Exosomes derived from HIV-1-infected cells contain trans-activation response element RNA. J Biol Chem. 2013; 288(27):20014-33. PMCID: PMC3707700.