Exosomes from RAW 264.7, a mouse macrophage cell line
- Highly pure
- Ready-to-use
- Well-characterized
- Fully functional
Products
| Catalog Number | Description | Size | Price | Quantity | Add to Cart | |||
|---|---|---|---|---|---|---|---|---|
| EXOP-165A-1 | Frozen exosomes (>1x10^6) from RAW 264.7 Mouse macrophage cell line | 50 µg | $544 |
|
||||
Overview
Overview
Save time with our ready-to-use purified exosomes Whether you’re looking for exosome standards, performing functional studies, evaluating biomarkers, or engineering exosomes for therapeutic delivery, you can get your research moving faster with our ready-to-use purified exosomes. Prepared with quality and care Like all of our purified exosomes, exosomes isolated from RAW 264.7, a mouse macrophage cell line, are carefully manufactured in SBI’s Palo Alto facilities. Starting with cells grown in Exo-FBS (our exosome-depleted FBS) to maximize purity, exosomes are isolated using ExoQuick-TC® and then quality checked by Western blot for the presence of CD63, and NanoSight for particle size and intactness. Provided in amounts > 1 x 106 exosomes (or 50 µg protein equivalent), all of our preps deliver functional exosomes.
- Highly pure
- Ready-to-use
- Well-characterized
- Fully functional
Available purified exosomes
| Cat.# | |
|---|---|
| Exosomes isolated from cancer cell lines | |
| EXOP-100A-1 | MCF-7 Human breast cancer, noninvasive cell line |
| EXOP-105A-1 | MDA-MB-231 Human breast cancer, aggressive/invasive/metastatic cell line |
| EXOP-115A-1 | PC-3 Human prostate cancer cells derived from metastatic cancer cell line |
| EXOP-120A-1 | A549 Human non-small cell lung cancer cell line |
| EXOP-125A-1 | H841 Human small cell lung cancer cell line |
| EXOP-130A-1 | H196 Human small cell lung cancer cell line |
| EXOP-135A-1 | DMS114 Human small cell lung cancer cell line |
| Exosomes isolated from stem cell lines | |
| EXOP-140A-1 | PCS-500-011 Human pre-adipose derived mesenchymal stem cell |
| EXOP-145A-1 | PCS-500-012 Human bone marrow-derived mesenchymal stem cell line |
| Exosomes isolated from immune-related cell lines | |
| EXOP-150A-1 | BC-3 Human B lymphocyte cell line |
| EXOP-155A-1 | Jurkat Clone E6-1 Human T lymphocyte cell line |
| EXOP-160A-1 | JM1 Human T pre-B lymphoblast cell line |
| EXOP-165A-1 | RAW 264.7 Mouse macrophage cell line |
| EXOP-200A-1 | JAWS II Mouse bone marrow immature dendritic cell line |
| Exosomes isolated from general cell lines | |
| EXOP-110A-1 | HEK293 Human embryonic kidney cell line |
| XPAK100EX-G | XPack-GFP-loaded HEK293 exosomes |
| Exosomes isolated from biofluid exosomes | |
| EXOP-500A-1 | Human pooled serum (healthy donors) |
| EXOP-510A-1 | Human pooled saliva (healthy donors) |
| EXOP-520A-1 | Human pooled urine (healthy donors) |
| EXOP-530A-1 | Human pooled CSF (healthy donors) |
| EXOP-540A-1 | Human breast milk, normal (single donor) |
| EXOP-550A-1 | Human biofluids ascites fluid (single donor) |
| Exosome isolated from mouse | |
| EXOP-560M-1 | Mouse Pooled Serum |
References
How It Works
Supporting Data
Supporting Data
Validated using Western blotting
All exosome preps are checked for the presence of CD63, a common exosome marker, via Western blotting (Figure 1).
Figure 1. All exosome preps contain CD63. Aliquots of purified exosomes from the cell lines and from human serum were lysed with either RIPA or M-PER buffer to make exosome protein lysates. Approximately 20 ug of protein for each sample was separated on a gradient SDS-PAGE and then transferred to nitrocellulose membranes. The membranes were probed for CD63 profiles using SBI’s anti-CD63 antibody (cat# EXOAB-CD63A-1) at a 1:1,000 dilution. Bands were detected using the secondary HRP-conjugated antibody at 1:10,000 and blots imaged. All purified exosome preparations were positive, immunoreactive for CD63 with the expected, variable banding patterns common to published exosome CD63 profiles.
FAQs
Resources
Citations
Related Products
Products
| Catalog Number | Description | Size | Price | Quantity | Add to Cart | |||
|---|---|---|---|---|---|---|---|---|
| EXOP-165A-1 | Frozen exosomes (>1x10^6) from RAW 264.7 Mouse macrophage cell line | 50 µg | $544 |
|
||||
Overview
Overview
Save time with our ready-to-use purified exosomes Whether you’re looking for exosome standards, performing functional studies, evaluating biomarkers, or engineering exosomes for therapeutic delivery, you can get your research moving faster with our ready-to-use purified exosomes. Prepared with quality and care Like all of our purified exosomes, exosomes isolated from RAW 264.7, a mouse macrophage cell line, are carefully manufactured in SBI’s Palo Alto facilities. Starting with cells grown in Exo-FBS (our exosome-depleted FBS) to maximize purity, exosomes are isolated using ExoQuick-TC® and then quality checked by Western blot for the presence of CD63, and NanoSight for particle size and intactness. Provided in amounts > 1 x 106 exosomes (or 50 µg protein equivalent), all of our preps deliver functional exosomes.
- Highly pure
- Ready-to-use
- Well-characterized
- Fully functional
Available purified exosomes
| Cat.# | |
|---|---|
| Exosomes isolated from cancer cell lines | |
| EXOP-100A-1 | MCF-7 Human breast cancer, noninvasive cell line |
| EXOP-105A-1 | MDA-MB-231 Human breast cancer, aggressive/invasive/metastatic cell line |
| EXOP-115A-1 | PC-3 Human prostate cancer cells derived from metastatic cancer cell line |
| EXOP-120A-1 | A549 Human non-small cell lung cancer cell line |
| EXOP-125A-1 | H841 Human small cell lung cancer cell line |
| EXOP-130A-1 | H196 Human small cell lung cancer cell line |
| EXOP-135A-1 | DMS114 Human small cell lung cancer cell line |
| Exosomes isolated from stem cell lines | |
| EXOP-140A-1 | PCS-500-011 Human pre-adipose derived mesenchymal stem cell |
| EXOP-145A-1 | PCS-500-012 Human bone marrow-derived mesenchymal stem cell line |
| Exosomes isolated from immune-related cell lines | |
| EXOP-150A-1 | BC-3 Human B lymphocyte cell line |
| EXOP-155A-1 | Jurkat Clone E6-1 Human T lymphocyte cell line |
| EXOP-160A-1 | JM1 Human T pre-B lymphoblast cell line |
| EXOP-165A-1 | RAW 264.7 Mouse macrophage cell line |
| EXOP-200A-1 | JAWS II Mouse bone marrow immature dendritic cell line |
| Exosomes isolated from general cell lines | |
| EXOP-110A-1 | HEK293 Human embryonic kidney cell line |
| XPAK100EX-G | XPack-GFP-loaded HEK293 exosomes |
| Exosomes isolated from biofluid exosomes | |
| EXOP-500A-1 | Human pooled serum (healthy donors) |
| EXOP-510A-1 | Human pooled saliva (healthy donors) |
| EXOP-520A-1 | Human pooled urine (healthy donors) |
| EXOP-530A-1 | Human pooled CSF (healthy donors) |
| EXOP-540A-1 | Human breast milk, normal (single donor) |
| EXOP-550A-1 | Human biofluids ascites fluid (single donor) |
| Exosome isolated from mouse | |
| EXOP-560M-1 | Mouse Pooled Serum |
References
How It Works
Supporting Data
Supporting Data
Validated using Western blotting
All exosome preps are checked for the presence of CD63, a common exosome marker, via Western blotting (Figure 1).
Figure 1. All exosome preps contain CD63. Aliquots of purified exosomes from the cell lines and from human serum were lysed with either RIPA or M-PER buffer to make exosome protein lysates. Approximately 20 ug of protein for each sample was separated on a gradient SDS-PAGE and then transferred to nitrocellulose membranes. The membranes were probed for CD63 profiles using SBI’s anti-CD63 antibody (cat# EXOAB-CD63A-1) at a 1:1,000 dilution. Bands were detected using the secondary HRP-conjugated antibody at 1:10,000 and blots imaged. All purified exosome preparations were positive, immunoreactive for CD63 with the expected, variable banding patterns common to published exosome CD63 profiles.