hsa-miRNome MicroRNA Profiling Kit (Human)
Products
Overview
Overview
Profile across the human miRNome
SBI has simplified qPCR profiling of human miRNAs by bringing together a comprehensive qPCR array of human miRNAs. Our Human hsa-miRNome MicroRNA Profiling Kit comes with all the reagents necessary to tag and convert small RNAs into quantifiable cDNA using the sensitive QuantiMir™ technology and a large set of human miRNA assays, including major and minor miR forms (1,113 mature miRNAs).
The kits include assays in three, pre-formatted 384-well assay plates with three endogenous reference miRNA controls on each plate (U1, U6, and RNU43). All microRNA assays are based on miRBase entries.
- Identify miRNA biomarkers and expression pattern signatures
- Rapidly tag and convert all small RNAs into detectable cDNA for qPCR
- Measure as little as picogram amounts of starting total RNA
- Conduct high-throughput screens of cell lines, tissues, and clinical samples
About the included QuantiMir Kit
When you’d like to profile miRNAs with qPCR, SBI’s QuantiMir™ Kit can get your samples ready. By efficiently converting all of your miRNA into cDNA, you can get accurate, sensitive, and quantitative qPCR data on the miRs in your sample. Great for understanding differential miRNA expression, simultaneously profiling siRNA and mRNA, and more.
- Simple and robust procedure
- Rapidly and efficiently convert all small RNAs into cDNAs for qPCR
- Suitable for high-throughput screening of clinical samples
- Sensitive and accurate
- Versatile—design your own miRNA assays
References
How It Works
How It Works
Using the hsa-miRNome MicroRNA Profiling Kit
First, prepare RNA for qPCR using the QuantiMir Kit
Highly efficient poly-A tailing and reverse transcription in a single reaction tube provides uniform cDNA synthesis of miRNAs. The optimized reaction conditions and buffer components maximize cDNA yield when starting with several micrograms down to picograms of input total RNA. The universal 3′ tag sequence incorporated during reverse transcription enables easily scalable and accurate miRNA expression analysis by qPCR—profile thousands of different miRNAs from a single reverse transcription reaction.
- Tag all small RNAs with a poly-A tail
- Anneal an oligo-dT adaptor
- Reverse transcribe to create first-strand cDNA
The result is a cDNA pool of anchor-tailed miRNAs that are ready for qPCR.
Then conduct qPCR assays
Get additional information about the array including miRNA sequences, miRbase accession numbers, and qPCR analysis templates in the Excel document, “Free Analysis Software for Human miRNome Ver. 15”
Supporting Data
Supporting Data
miRNome Profiler Performance Data
Figure 1. Accurately normalize your microRNA expression profiling data using three reference controls—U6 snRNA, RNU1A, and RNU43.
Figure 2. Get high reproducibility—excellent technical replicates across 8-logs. Reliable replicates from plate-to-plate enable precise comparisons of numerous samples across large microRNA profiling studies.
FAQs
Resources
Citations
Related Products
Products
Overview
Overview
Profile across the human miRNome
SBI has simplified qPCR profiling of human miRNAs by bringing together a comprehensive qPCR array of human miRNAs. Our Human hsa-miRNome MicroRNA Profiling Kit comes with all the reagents necessary to tag and convert small RNAs into quantifiable cDNA using the sensitive QuantiMir™ technology and a large set of human miRNA assays, including major and minor miR forms (1,113 mature miRNAs).
The kits include assays in three, pre-formatted 384-well assay plates with three endogenous reference miRNA controls on each plate (U1, U6, and RNU43). All microRNA assays are based on miRBase entries.
- Identify miRNA biomarkers and expression pattern signatures
- Rapidly tag and convert all small RNAs into detectable cDNA for qPCR
- Measure as little as picogram amounts of starting total RNA
- Conduct high-throughput screens of cell lines, tissues, and clinical samples
About the included QuantiMir Kit
When you’d like to profile miRNAs with qPCR, SBI’s QuantiMir™ Kit can get your samples ready. By efficiently converting all of your miRNA into cDNA, you can get accurate, sensitive, and quantitative qPCR data on the miRs in your sample. Great for understanding differential miRNA expression, simultaneously profiling siRNA and mRNA, and more.
- Simple and robust procedure
- Rapidly and efficiently convert all small RNAs into cDNAs for qPCR
- Suitable for high-throughput screening of clinical samples
- Sensitive and accurate
- Versatile—design your own miRNA assays
References
How It Works
How It Works
Using the hsa-miRNome MicroRNA Profiling Kit
First, prepare RNA for qPCR using the QuantiMir Kit
Highly efficient poly-A tailing and reverse transcription in a single reaction tube provides uniform cDNA synthesis of miRNAs. The optimized reaction conditions and buffer components maximize cDNA yield when starting with several micrograms down to picograms of input total RNA. The universal 3′ tag sequence incorporated during reverse transcription enables easily scalable and accurate miRNA expression analysis by qPCR—profile thousands of different miRNAs from a single reverse transcription reaction.
- Tag all small RNAs with a poly-A tail
- Anneal an oligo-dT adaptor
- Reverse transcribe to create first-strand cDNA
The result is a cDNA pool of anchor-tailed miRNAs that are ready for qPCR.
Then conduct qPCR assays
Get additional information about the array including miRNA sequences, miRbase accession numbers, and qPCR analysis templates in the Excel document, “Free Analysis Software for Human miRNome Ver. 15”
Supporting Data
Supporting Data
miRNome Profiler Performance Data
Figure 1. Accurately normalize your microRNA expression profiling data using three reference controls—U6 snRNA, RNU1A, and RNU43.
Figure 2. Get high reproducibility—excellent technical replicates across 8-logs. Reliable replicates from plate-to-plate enable precise comparisons of numerous samples across large microRNA profiling studies.