NF-κB/293/GFP-Luc™ Transcriptional Reporter Cell Line
Monitor NF-κB signaling in vitro and screen for genetic and/or small molecule inhibitors and activators, and more – sort with GFP and quantify with luciferase
- Study NF-κB signaling in 293 TN cells
- Perform live cell imaging or FACS (300-fold over background NF-κB-dependent GFP signal)
- Measure pathway activity with luciferase
- Compatible with a variety of screening methods including small molecule and RNAi
Products
| Catalog Number | Description | Size | Price | Quantity | Add to Cart | |||
|---|---|---|---|---|---|---|---|---|
| TR860A-1 | NF-kB/293/GFP-Luc Transcriptional Reporter Cell Line | >2 x 10^6 Cells | $3258 |
|
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Overview
Overview
Monitor NF-κB signaling in Jurkat cells Speed your studies with this pre-built 293 TN-based cell line for monitoring NF-κB signaling in real time. We’ve already integrated an expression cassette that includes NF-κB-responsive transcriptional elements upstream of a minimal CMV promoter (mCMV)-GFP-luciferase cassette. Expression of GFP (up to 300-fold over background) and luciferase only occurs in the presence of active NF-κB signaling, enabling screening for genetic and/or small molecule inhibitors and activators of the NF-κB signaling pathway. Use GFP fluorescence for live cell imaging and FACS, or luciferase activity to quantitatively measure activity of the signaling pathway.- Study NF-κB signaling in 293 TN cells
- Perform live cell imaging or FACS (300-fold over background NF-κB-dependent GFP signal)
- Measure pathway activity with luciferase
- Compatible with a variety of screening methods including small molecule and RNAi
References
How It Works
Supporting Data
Supporting Data
See the NF-κB/293/GFP-luc Transcriptional Reporter Cell Line in action
Monitor NF-κB in real time with GFP
Quantitate NF-κB Activation with luciferase assays
FAQs
Resources
Citations
Related Products
NF-κB/293/GFP-Luc™ Transcriptional Reporter Cell Line
$3,258.00
Products
| Catalog Number | Description | Size | Price | Quantity | Add to Cart | |||
|---|---|---|---|---|---|---|---|---|
| TR860A-1 | NF-kB/293/GFP-Luc Transcriptional Reporter Cell Line | >2 x 10^6 Cells | $3258 |
|
||||
Overview
Overview
Monitor NF-κB signaling in Jurkat cells Speed your studies with this pre-built 293 TN-based cell line for monitoring NF-κB signaling in real time. We’ve already integrated an expression cassette that includes NF-κB-responsive transcriptional elements upstream of a minimal CMV promoter (mCMV)-GFP-luciferase cassette. Expression of GFP (up to 300-fold over background) and luciferase only occurs in the presence of active NF-κB signaling, enabling screening for genetic and/or small molecule inhibitors and activators of the NF-κB signaling pathway. Use GFP fluorescence for live cell imaging and FACS, or luciferase activity to quantitatively measure activity of the signaling pathway.- Study NF-κB signaling in 293 TN cells
- Perform live cell imaging or FACS (300-fold over background NF-κB-dependent GFP signal)
- Measure pathway activity with luciferase
- Compatible with a variety of screening methods including small molecule and RNAi
References
How It Works
Supporting Data
Supporting Data
See the NF-κB/293/GFP-luc Transcriptional Reporter Cell Line in action
Monitor NF-κB in real time with GFP
Quantitate NF-κB Activation with luciferase assays