pGreenZeo-mCMV Differentiation Reporter Negative Control

Run your pGreenZeo projects with confidence with the addition of this negative control that drives dscGFP and zeomycin resistance with a minimal CMV promoter

Products

Catalog Number Description Size Price Quantity Add to Cart
SR500PA-1 pGreenZeo-mCMV Plasmid (negative control) 10 µg $492
- +
SR500VA-1 pGreenZeo-mCMV Virus (negative control) >2 x 10^6 IFUs $630
- +

Overview

Overview

Supporting your studies with ready-to-go controls

No need to make a negative control for your pGreenZeo projects—SBI’s already built one for you. The GreenZeo cassette is driven by a minimal CMV promoter with the dscGFP (destabilized copGFP, 2-hour half-life) and zeomycin marker co-expression mediated by a T2A element. This configuration provides a control for background levels of GFP and zeomycin expression in the absence of enhancer elements, such as the ones used in our Pluripotency Reporters.

Choose the pluripotency reporter that’s right for you

Pluripotency Reporters

Catalog #SpeciesPromoter/enhancer elementReporter
SR10033PA/VA-1HumanOct-4pGreenZeo
SR10043PA/VA-1HumanOct-4pRedZeo
SR10053PA/VA-1HumanOct-4pRedTK
SR10029PA/VA-1MouseOct-4pGreenZeo
SR10045PA/VA-1MouseOct-4pRedZeo
SR10054PA/VA-1MouseOct-4pRedTK
SR10030PA/VA-1HumanNanogpGreenZeo
SR10042PA/VA-1HumanNanogpRedZeo
SR10055PA/VA-1HumanNanogpRedTK
SR10031PA/VA-1MouseNanogpGreenZeo
SR10044PA/VA-1MouseNanogpRedZeo
SR10056PA/VA-1MouseNanogpRedTK

References

How It Works

Supporting Data

Supporting Data

See some of our differentiation reporters in action

SBI’s differentiation reporters are used in a number of papers. The data shown below are just one example (from Ravin R, Hoeppner DJ, Munno DM, Carmel L, Sullivan J, Levitt DL, Miller JL, Athaide C, Panchision DM, McKay RD. Potency and fate specification in CNS stem cell populations in vitro. Cell Stem Cell. 2008 Dec 4; 3(6):670-80. PMID: 19041783)

Live imaging of neuronal differentiation

Figure 1. Live imaging of neuronal differentiation. Ravin, et al, used SBI’s Human Nestin pGreenFire Differentiation Reporter (Cat.# SR10016VA-1), which drives GFP expression from the glial fibrillary acidic protein promoter, to watch human neural stem cells differentiate into a network of mature neurons, oligodendrocytes, and astrocytes over the course of seven days. The periodic “flashes” seen in this video correspond to fluorescent photos taken of the growing cells to identify the GFP signals. The final photo taken after the network formation is shown below the video (color added). Among the network of neurons, only the astrocytes are bright green, demonsthumaning the specificity of SBI’s human Nestin pGreenFire Differentiation Reporter.

Simultaneously track multiple lineages from iPS and progenitor cells

Figure 2. Simultaneously track multiple lineages from iPS and progenitor cells.

See SBI’s differentiation reporters in action

Figure 3. Additional differentiation reporter data.

FAQs

Resources

Citations

Products

Catalog Number Description Size Price Quantity Add to Cart
SR500PA-1 pGreenZeo-mCMV Plasmid (negative control) 10 µg $492
- +
SR500VA-1 pGreenZeo-mCMV Virus (negative control) >2 x 10^6 IFUs $630
- +

Overview

Overview

Supporting your studies with ready-to-go controls

No need to make a negative control for your pGreenZeo projects—SBI’s already built one for you. The GreenZeo cassette is driven by a minimal CMV promoter with the dscGFP (destabilized copGFP, 2-hour half-life) and zeomycin marker co-expression mediated by a T2A element. This configuration provides a control for background levels of GFP and zeomycin expression in the absence of enhancer elements, such as the ones used in our Pluripotency Reporters.

Choose the pluripotency reporter that’s right for you

Pluripotency Reporters

Catalog #SpeciesPromoter/enhancer elementReporter
SR10033PA/VA-1HumanOct-4pGreenZeo
SR10043PA/VA-1HumanOct-4pRedZeo
SR10053PA/VA-1HumanOct-4pRedTK
SR10029PA/VA-1MouseOct-4pGreenZeo
SR10045PA/VA-1MouseOct-4pRedZeo
SR10054PA/VA-1MouseOct-4pRedTK
SR10030PA/VA-1HumanNanogpGreenZeo
SR10042PA/VA-1HumanNanogpRedZeo
SR10055PA/VA-1HumanNanogpRedTK
SR10031PA/VA-1MouseNanogpGreenZeo
SR10044PA/VA-1MouseNanogpRedZeo
SR10056PA/VA-1MouseNanogpRedTK

References

How It Works

Supporting Data

Supporting Data

See some of our differentiation reporters in action

SBI’s differentiation reporters are used in a number of papers. The data shown below are just one example (from Ravin R, Hoeppner DJ, Munno DM, Carmel L, Sullivan J, Levitt DL, Miller JL, Athaide C, Panchision DM, McKay RD. Potency and fate specification in CNS stem cell populations in vitro. Cell Stem Cell. 2008 Dec 4; 3(6):670-80. PMID: 19041783)

Live imaging of neuronal differentiation

Figure 1. Live imaging of neuronal differentiation. Ravin, et al, used SBI’s Human Nestin pGreenFire Differentiation Reporter (Cat.# SR10016VA-1), which drives GFP expression from the glial fibrillary acidic protein promoter, to watch human neural stem cells differentiate into a network of mature neurons, oligodendrocytes, and astrocytes over the course of seven days. The periodic “flashes” seen in this video correspond to fluorescent photos taken of the growing cells to identify the GFP signals. The final photo taken after the network formation is shown below the video (color added). Among the network of neurons, only the astrocytes are bright green, demonsthumaning the specificity of SBI’s human Nestin pGreenFire Differentiation Reporter.

Simultaneously track multiple lineages from iPS and progenitor cells

Figure 2. Simultaneously track multiple lineages from iPS and progenitor cells.

See SBI’s differentiation reporters in action

Figure 3. Additional differentiation reporter data.

FAQs

Citations