pGreenZeo-mCMV Differentiation Reporter Negative Control
Products
| Catalog Number | Description | Size | Price | Quantity | Add to Cart | |||
|---|---|---|---|---|---|---|---|---|
| SR500PA-1 | pGreenZeo-mCMV Plasmid (negative control) | 10 µg | $492 |
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| SR500VA-1 | pGreenZeo-mCMV Virus (negative control) | >2 x 10^6 IFUs | $630 |
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Overview
Overview
Supporting your studies with ready-to-go controls
No need to make a negative control for your pGreenZeo projects—SBI’s already built one for you. The GreenZeo cassette is driven by a minimal CMV promoter with the dscGFP (destabilized copGFP, 2-hour half-life) and zeomycin marker co-expression mediated by a T2A element. This configuration provides a control for background levels of GFP and zeomycin expression in the absence of enhancer elements, such as the ones used in our Pluripotency Reporters.
Choose the pluripotency reporter that’s right for you
Pluripotency Reporters
| Catalog # | Species | Promoter/enhancer element | Reporter |
|---|---|---|---|
| SR10033PA/VA-1 | Human | Oct-4 | pGreenZeo |
| SR10043PA/VA-1 | Human | Oct-4 | pRedZeo |
| SR10053PA/VA-1 | Human | Oct-4 | pRedTK |
| SR10029PA/VA-1 | Mouse | Oct-4 | pGreenZeo |
| SR10045PA/VA-1 | Mouse | Oct-4 | pRedZeo |
| SR10054PA/VA-1 | Mouse | Oct-4 | pRedTK |
| SR10030PA/VA-1 | Human | Nanog | pGreenZeo |
| SR10042PA/VA-1 | Human | Nanog | pRedZeo |
| SR10055PA/VA-1 | Human | Nanog | pRedTK |
| SR10031PA/VA-1 | Mouse | Nanog | pGreenZeo |
| SR10044PA/VA-1 | Mouse | Nanog | pRedZeo |
| SR10056PA/VA-1 | Mouse | Nanog | pRedTK |
References
How It Works
Supporting Data
Supporting Data
See some of our differentiation reporters in action
SBI’s differentiation reporters are used in a number of papers. The data shown below are just one example (from Ravin R, Hoeppner DJ, Munno DM, Carmel L, Sullivan J, Levitt DL, Miller JL, Athaide C, Panchision DM, McKay RD. Potency and fate specification in CNS stem cell populations in vitro. Cell Stem Cell. 2008 Dec 4; 3(6):670-80. PMID: 19041783)
Figure 1. Live imaging of neuronal differentiation. Ravin, et al, used SBI’s Human Nestin pGreenFire Differentiation Reporter (Cat.# SR10016VA-1), which drives GFP expression from the glial fibrillary acidic protein promoter, to watch human neural stem cells differentiate into a network of mature neurons, oligodendrocytes, and astrocytes over the course of seven days. The periodic “flashes” seen in this video correspond to fluorescent photos taken of the growing cells to identify the GFP signals. The final photo taken after the network formation is shown below the video (color added). Among the network of neurons, only the astrocytes are bright green, demonsthumaning the specificity of SBI’s human Nestin pGreenFire Differentiation Reporter.
Figure 2. Simultaneously track multiple lineages from iPS and progenitor cells.
Figure 3. Additional differentiation reporter data.
FAQs
Resources
Citations
Related Products
Products
| Catalog Number | Description | Size | Price | Quantity | Add to Cart | |||
|---|---|---|---|---|---|---|---|---|
| SR500PA-1 | pGreenZeo-mCMV Plasmid (negative control) | 10 µg | $492 |
|
||||
| SR500VA-1 | pGreenZeo-mCMV Virus (negative control) | >2 x 10^6 IFUs | $630 |
|
||||
Overview
Overview
Supporting your studies with ready-to-go controls
No need to make a negative control for your pGreenZeo projects—SBI’s already built one for you. The GreenZeo cassette is driven by a minimal CMV promoter with the dscGFP (destabilized copGFP, 2-hour half-life) and zeomycin marker co-expression mediated by a T2A element. This configuration provides a control for background levels of GFP and zeomycin expression in the absence of enhancer elements, such as the ones used in our Pluripotency Reporters.
Choose the pluripotency reporter that’s right for you
Pluripotency Reporters
| Catalog # | Species | Promoter/enhancer element | Reporter |
|---|---|---|---|
| SR10033PA/VA-1 | Human | Oct-4 | pGreenZeo |
| SR10043PA/VA-1 | Human | Oct-4 | pRedZeo |
| SR10053PA/VA-1 | Human | Oct-4 | pRedTK |
| SR10029PA/VA-1 | Mouse | Oct-4 | pGreenZeo |
| SR10045PA/VA-1 | Mouse | Oct-4 | pRedZeo |
| SR10054PA/VA-1 | Mouse | Oct-4 | pRedTK |
| SR10030PA/VA-1 | Human | Nanog | pGreenZeo |
| SR10042PA/VA-1 | Human | Nanog | pRedZeo |
| SR10055PA/VA-1 | Human | Nanog | pRedTK |
| SR10031PA/VA-1 | Mouse | Nanog | pGreenZeo |
| SR10044PA/VA-1 | Mouse | Nanog | pRedZeo |
| SR10056PA/VA-1 | Mouse | Nanog | pRedTK |
References
How It Works
Supporting Data
Supporting Data
See some of our differentiation reporters in action
SBI’s differentiation reporters are used in a number of papers. The data shown below are just one example (from Ravin R, Hoeppner DJ, Munno DM, Carmel L, Sullivan J, Levitt DL, Miller JL, Athaide C, Panchision DM, McKay RD. Potency and fate specification in CNS stem cell populations in vitro. Cell Stem Cell. 2008 Dec 4; 3(6):670-80. PMID: 19041783)
Figure 1. Live imaging of neuronal differentiation. Ravin, et al, used SBI’s Human Nestin pGreenFire Differentiation Reporter (Cat.# SR10016VA-1), which drives GFP expression from the glial fibrillary acidic protein promoter, to watch human neural stem cells differentiate into a network of mature neurons, oligodendrocytes, and astrocytes over the course of seven days. The periodic “flashes” seen in this video correspond to fluorescent photos taken of the growing cells to identify the GFP signals. The final photo taken after the network formation is shown below the video (color added). Among the network of neurons, only the astrocytes are bright green, demonsthumaning the specificity of SBI’s human Nestin pGreenFire Differentiation Reporter.
Figure 2. Simultaneously track multiple lineages from iPS and progenitor cells.
Figure 3. Additional differentiation reporter data.