pLL-CMV-rFLuc-T2A-mRFP-mPGK-Puro Lenti-Labeler™ Lentivector Plasmid & Pre-packaged Virus

Reliably co-label the cells of your choice with rFLUc and mRFP driven by the strong CMV promoter (with puro selection) - great for tracking cells, HTS assays, and more
  • Proven—leverages SBI’s third generation lentivector technology for optimal virus titers
  • Validated—sequence-verified from LTR to LTR and expression-verified in HT-1080 cells
  • Flexible—available in a range of fluorescent markers, selection markers, and promoters (Table 1)

Products

Catalog Number Description Size Price Quantity Add to Cart
LL320PA-1 pLL-CMV-rFLuc-T2A-mRFP-mPGK-Puro (Lenti-Labeler™ plasmid) 10 µg $643
- +
LL320VA-1 pLL-CMV-rFLuc-T2A-mRFP-mPGK-Puro (Lenti-Labeler™ virus) >2x10^6 IFUs $643
- +

Overview

Overview

Reliable cell labeling, delivered

SBI’s family of Lenti-Labeler™ constructs facilitate a wide range of studies—including cell tracking, high-throughput assays, and more—by enabling efficient and reliable labeling of your cells. The pLL-CMV-rFLuc-T2A-mRFP-mPGK-Puro Lenti-Labeler construct co-expresses rFLuc and mRFP from the CMV promoter, which delivers strong expression in most commonly-used cell lines (HeLa, HEK293, HT-1080, etc.), and co-expresses the puromycin resistance gene for selection in vitro prior to in vivo use. Available as either fully propagatable, sequence-verified plasmid DNA (Cat.# LL320PA-1) or ready-to-transduce pre-packaged lentivirus (Cat.# LL320VA-1), SBI’s pLL-CMV-rFLuc-T2A-mRFP-mPGK-Puro Lenti-Labeler is designed for reliability, so you can get to valuable insights faster.

pLL-CMV-rFLuc-T2A-mRFP-mPGK-Puro Lenti-Labeler
  • Proven—leverages SBI’s third generation lentivector technology for optimal virus titers
  • Validated—sequence-verified from LTR to LTR and expression-verified in HT-1080 cells
  • Flexible—available in a range of fluorescent markers, selection markers, and promoters (Table 1)

While the pLL-CMV-rFLuc-T2A-mRFP-mPGK-Puro Lenti-Labeler construct is compatible with most second and third generation packaging plasmid mixes, SBI recommends the use of pPACKH1 (Cat #LV500A-1) and TransDux MAX Transduction Reagent (Cat #LV860A-1) to achieve optimal virus titers and infection of target cells.

Find the right Lenti-Labeler construct for your studies

SBI offers a Lenti-Labeler constructs with a range of selection markers, reporter genes, and two different promoters.

Table 1. Available Lenti-Labeler Constructs

Cat. #ConstructPromoterReporterSelection
LL100PA-1/LL100VA-1pLL-CMV-GFP-T2A-PuroCMV
GFPPuromycin
LL105PA-1/LL105VA-1pLL-CMV-GFP-T2A-Blast

CMVGFPBlasticidin
LL110PA-1/LL110VA-1pLL-CMV-RFP-T2A-PuroCMVRFPPuromycin
LL115PA-1/LL115VA-1pLL-CMV-RFP-T2A-BlastCMVRFPBlasticidin
LL120PA-1/LL120VA-1pLL-CMV-BFP-T2A-PuroCMVBFPPuromycin
LL125PA-1/LL125VA-1pLL-CMV-BFP-T2A-BlastCMVBFPBlasticidin
LL150PA-1/LL150VA-1pLL-CMV-Luciferase-T2A-PuroCMVLuciferasePuromycin
LL200PA-1/LL200VA-1pLL-EF1α-GFP-T2A-PuroEF1αGFPPuromycin
LL205PA-1/LL205VA-1pLL-EF1α-GFP-T2A-BlastEF1αGFPBlasticidin
LL210PA-1/LL210VA-1pLL-EF1α-RFP-T2A-PuroEF1αRFPPuromycin
LL215PA-1/LL215VA-1pLL-EF1α-RFP-T2A-BlastEF1αRFPBlasticidin
LL220PA-1/LL220VA-1pLL-EF1α-BFP-T2A-PuroEF1αBFPPuromycin
LL225PA-1/LL225VA-1pLL-EF1α-BFP-T2A-BlastEF1αBFPBlasticidin
LL250PA-1/LL250VA-1pLL-EF1α-Luciferase-T2A-PuroEF1αLuciferasePuromycin
LL300PA-1/LL300VA-1pLL-CMV-rFLuc-T2A-GFPCMVLuciferase & GFPN/A
LL310PA-1/LL310VA-1pLL-CMV-rFLuc-T2A-GFP-mPGK-Puro CMVLuciferase & GFPPuromycin
LL320PA-1/LL320VA-1pLL-CMV-rFLuc-T2A-mRFP-mPGK-Puro CMVLuciferase & RFPPuromycin
LL410PA-1/LL410VA-1pLL-EF1a-rFLuc-T2A-GFP-mPGK-Puro EF1αLuciferase & GFPPuromycin
LL420PA-1/LL420VA-1pLL-EF1a-rFLuc-T2A-mRFP-mPGK-Puro EF1αLuciferase & RFPPuromycin

References

How It Works

Supporting Data

Supporting Data

SBI’s Lenti-Labelers get your cells labeled efficiently, reliably

pLL-CMV-GFP-T2A-Puro Lenti-Labeler

Figure 1. SBI Lenti-Labeler constructs reliably and efficiently label cells. Comparison of the number of fluorescently-labeled cells to the total number of cells seen in the corresponding phase contrast images reveals the high labeling efficiency of SBI’s Lenti-Labeler constructs.

Double Reporter Lenti-Labelers

Figure 2. Dual reporter Lenti-Labeler vectors deliver robust fluorescence (A) and luminescence (B) signals. Data was obtained three days after infection of MDA-MB-231 cells with the dual reporter Lenti-Labeler Lentivirus CMV-rFLuc-T2A-GFP-mPGK-Puro (Cat #LL310VA-1). Infection efficiency was maximized by using SBI’s PEG-it Reagent (Cat.# LV810A-1) to concentrate the Lenti-Labeler Lentivirus prior to infection.

FAQs

Resources

Citations

pLL-CMV-rFLuc-T2A-mRFP-mPGK-Puro Lenti-Labeler™ Lentivector Plasmid & Pre-packaged Virus $643.00

Products

Catalog Number Description Size Price Quantity Add to Cart
LL320PA-1 pLL-CMV-rFLuc-T2A-mRFP-mPGK-Puro (Lenti-Labeler™ plasmid) 10 µg $643
- +
LL320VA-1 pLL-CMV-rFLuc-T2A-mRFP-mPGK-Puro (Lenti-Labeler™ virus) >2x10^6 IFUs $643
- +

Overview

Overview

Reliable cell labeling, delivered

SBI’s family of Lenti-Labeler™ constructs facilitate a wide range of studies—including cell tracking, high-throughput assays, and more—by enabling efficient and reliable labeling of your cells. The pLL-CMV-rFLuc-T2A-mRFP-mPGK-Puro Lenti-Labeler construct co-expresses rFLuc and mRFP from the CMV promoter, which delivers strong expression in most commonly-used cell lines (HeLa, HEK293, HT-1080, etc.), and co-expresses the puromycin resistance gene for selection in vitro prior to in vivo use. Available as either fully propagatable, sequence-verified plasmid DNA (Cat.# LL320PA-1) or ready-to-transduce pre-packaged lentivirus (Cat.# LL320VA-1), SBI’s pLL-CMV-rFLuc-T2A-mRFP-mPGK-Puro Lenti-Labeler is designed for reliability, so you can get to valuable insights faster.

pLL-CMV-rFLuc-T2A-mRFP-mPGK-Puro Lenti-Labeler
  • Proven—leverages SBI’s third generation lentivector technology for optimal virus titers
  • Validated—sequence-verified from LTR to LTR and expression-verified in HT-1080 cells
  • Flexible—available in a range of fluorescent markers, selection markers, and promoters (Table 1)

While the pLL-CMV-rFLuc-T2A-mRFP-mPGK-Puro Lenti-Labeler construct is compatible with most second and third generation packaging plasmid mixes, SBI recommends the use of pPACKH1 (Cat #LV500A-1) and TransDux MAX Transduction Reagent (Cat #LV860A-1) to achieve optimal virus titers and infection of target cells.

Find the right Lenti-Labeler construct for your studies

SBI offers a Lenti-Labeler constructs with a range of selection markers, reporter genes, and two different promoters.

Table 1. Available Lenti-Labeler Constructs

Cat. #ConstructPromoterReporterSelection
LL100PA-1/LL100VA-1pLL-CMV-GFP-T2A-PuroCMV
GFPPuromycin
LL105PA-1/LL105VA-1pLL-CMV-GFP-T2A-Blast

CMVGFPBlasticidin
LL110PA-1/LL110VA-1pLL-CMV-RFP-T2A-PuroCMVRFPPuromycin
LL115PA-1/LL115VA-1pLL-CMV-RFP-T2A-BlastCMVRFPBlasticidin
LL120PA-1/LL120VA-1pLL-CMV-BFP-T2A-PuroCMVBFPPuromycin
LL125PA-1/LL125VA-1pLL-CMV-BFP-T2A-BlastCMVBFPBlasticidin
LL150PA-1/LL150VA-1pLL-CMV-Luciferase-T2A-PuroCMVLuciferasePuromycin
LL200PA-1/LL200VA-1pLL-EF1α-GFP-T2A-PuroEF1αGFPPuromycin
LL205PA-1/LL205VA-1pLL-EF1α-GFP-T2A-BlastEF1αGFPBlasticidin
LL210PA-1/LL210VA-1pLL-EF1α-RFP-T2A-PuroEF1αRFPPuromycin
LL215PA-1/LL215VA-1pLL-EF1α-RFP-T2A-BlastEF1αRFPBlasticidin
LL220PA-1/LL220VA-1pLL-EF1α-BFP-T2A-PuroEF1αBFPPuromycin
LL225PA-1/LL225VA-1pLL-EF1α-BFP-T2A-BlastEF1αBFPBlasticidin
LL250PA-1/LL250VA-1pLL-EF1α-Luciferase-T2A-PuroEF1αLuciferasePuromycin
LL300PA-1/LL300VA-1pLL-CMV-rFLuc-T2A-GFPCMVLuciferase & GFPN/A
LL310PA-1/LL310VA-1pLL-CMV-rFLuc-T2A-GFP-mPGK-Puro CMVLuciferase & GFPPuromycin
LL320PA-1/LL320VA-1pLL-CMV-rFLuc-T2A-mRFP-mPGK-Puro CMVLuciferase & RFPPuromycin
LL410PA-1/LL410VA-1pLL-EF1a-rFLuc-T2A-GFP-mPGK-Puro EF1αLuciferase & GFPPuromycin
LL420PA-1/LL420VA-1pLL-EF1a-rFLuc-T2A-mRFP-mPGK-Puro EF1αLuciferase & RFPPuromycin

References

How It Works

Supporting Data

Supporting Data

SBI’s Lenti-Labelers get your cells labeled efficiently, reliably

pLL-CMV-GFP-T2A-Puro Lenti-Labeler

Figure 1. SBI Lenti-Labeler constructs reliably and efficiently label cells. Comparison of the number of fluorescently-labeled cells to the total number of cells seen in the corresponding phase contrast images reveals the high labeling efficiency of SBI’s Lenti-Labeler constructs.

Double Reporter Lenti-Labelers

Figure 2. Dual reporter Lenti-Labeler vectors deliver robust fluorescence (A) and luminescence (B) signals. Data was obtained three days after infection of MDA-MB-231 cells with the dual reporter Lenti-Labeler Lentivirus CMV-rFLuc-T2A-GFP-mPGK-Puro (Cat #LL310VA-1). Infection efficiency was maximized by using SBI’s PEG-it Reagent (Cat.# LV810A-1) to concentrate the Lenti-Labeler Lentivirus prior to infection.

FAQs

Citations