pLL-EF1a-Luciferase-T2A-Puro Lenti-Labeler™ Lentivector Plasmid & Pre-packaged Virus

Overview

Reliable cell labeling, delivered

SBI’s family of Lenti-Labeler™ constructs facilitate a wide range of studies—including cell tracking, high-throughput assays, and more—by enabling efficient and reliable labeling of your cells. The pLL-EF1α-Luciferase-T2A-Puro Lenti-Labeler™ construct expresses luciferase from the EF1α promoter, which delivers moderate expression in most cell types (including primary and stem cells), and co-expresses the puromycin resistance gene for selection in vitro prior to in vivo use. Available as either fully propagatable, sequence-verified plasmid DNA (Cat.# LL250PA-1) or ready-to-transduce pre-packaged lentivirus (Cat.# LL250VA-1), SBI’s pLL-EF1α-Luciferase-T2A-Puro Lenti-Labeler™ is designed for reliability, so you can get to valuable insights faster.

• Proven—leverages SBI’s third generation lentivector technology for optimal virus titers
• Validated—sequence-verified from LTR to LTR and expression-verified in HT-1080 cells
• Flexible—available in a range of fluorescent markers, selection markers, and promoters (Table 1)

While the pLL-EF1α-Luciferase-T2A-Puro Lenti-Labeler construct is compatible with most second and third generation packaging plasmid mixes, SBI recommends the use of pPACKH1 (Cat #LV500A-1) and TransDux MAX Transduction Reagent (Cat #LV860A-1) to achieve optimal virus titers and infection of target cells.

Find the right Lenti-Labeler construct for your studies

SBI offers a Lenti-Labeler constructs with a range of selection markers, reporter genes, and two different promoters.

Table 1. Available Lenti-Labeler Constructs

Cat. #	Construct	Promoter	Reporter	Selection
LL100PA-1/LL100VA-1	pLL-CMV-GFP-T2A-Puro	CMV	GFP	Puromycin
LL105PA-1/LL105VA-1	pLL-CMV-GFP-T2A-Blast	CMV	GFP	Blasticidin
LL110PA-1/LL110VA-1	pLL-CMV-RFP-T2A-Puro	CMV	RFP	Puromycin
LL115PA-1/LL115VA-1	pLL-CMV-RFP-T2A-Blast	CMV	RFP	Blasticidin
LL120PA-1/LL120VA-1	pLL-CMV-BFP-T2A-Puro	CMV	BFP	Puromycin
LL125PA-1/LL125VA-1	pLL-CMV-BFP-T2A-Blast	CMV	BFP	Blasticidin
LL150PA-1/LL150VA-1	pLL-CMV-Luciferase-T2A-Puro	CMV	Luciferase	Puromycin
LL200PA-1/LL200VA-1	pLL-EF1α-GFP-T2A-Puro	EF1α	GFP	Puromycin
LL205PA-1/LL205VA-1	pLL-EF1α-GFP-T2A-Blast	EF1α	GFP	Blasticidin
LL210PA-1/LL210VA-1	pLL-EF1α-RFP-T2A-Puro	EF1α	RFP	Puromycin
LL215PA-1/LL215VA-1	pLL-EF1α-RFP-T2A-Blast	EF1α	RFP	Blasticidin
LL220PA-1/LL220VA-1	pLL-EF1α-BFP-T2A-Puro	EF1α	BFP	Puromycin
LL225PA-1/LL225VA-1	pLL-EF1α-BFP-T2A-Blast	EF1α	BFP	Blasticidin
LL250PA-1/LL250VA-1	pLL-EF1α-Luciferase-T2A-Puro	EF1α	Luciferase	Puromycin
LL300PA-1/LL300VA-1	pLL-CMV-rFLuc-T2A-GFP	CMV	Luciferase & GFP	N/A
LL310PA-1/LL310VA-1	pLL-CMV-rFLuc-T2A-GFP-mPGK-Puro	CMV	Luciferase & GFP	Puromycin
LL320PA-1/LL320VA-1	pLL-CMV-rFLuc-T2A-mRFP-mPGK-Puro	CMV	Luciferase & RFP	Puromycin
LL410PA-1/LL410VA-1	pLL-EF1a-rFLuc-T2A-GFP-mPGK-Puro	EF1α	Luciferase & GFP	Puromycin
LL420PA-1/LL420VA-1	pLL-EF1a-rFLuc-T2A-mRFP-mPGK-Puro	EF1α	Luciferase & RFP	Puromycin