pLL-EF1a-RFP-T2A-Blast Lenti-Labeler™ Lentivector Plasmid & Pre-packaged Virus
- Proven—leverages SBI’s third generation lentivector technology for optimal virus titers
- Validated—sequence-verified from LTR to LTR and expression-verified in HT-1080 cells
- Flexible—available in a range of fluorescent markers, selection markers, and promoters (Table 1)
Products
Catalog Number | Description | Size | Price | Quantity | Add to Cart | |||
---|---|---|---|---|---|---|---|---|
LL215PA-1 | pLL-EF1a-RFP-T2A-Blast (Lenti-Labeler™ plasmid) | 10 µg | $635 |
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LL215VA-1 | pLL-EF1a-RFP-T2A-Blast (Lenti-Labeler™ virus) | >2x10^6 IFUs | $635 |
|
Overview
Overview
Reliable cell labeling, delivered
SBI’s family of Lenti-Labeler™ constructs facilitate a wide range of studies—including cell tracking, high-throughput assays, and more—by enabling efficient and reliable labeling of your cells. The pLL-EF1α-RFP-T2A-Blast Lenti-Labeler™ construct expresses RFP from the EF1α promoter, which delivers moderate expression in most cell types (including primary and stem cells), and co-expresses the blasticidin resistance gene for selection in vitro prior to in vivo use. Available as either fully propagatable, sequence-verified plasmid DNA (Cat.# LL215PA-1) or ready-to-transduce pre-packaged lentivirus (Cat.# LL215VA-1), SBI’s pLL-EF1α-RFP-T2A-Blast Lenti-Labeler™ is designed for reliability, so you can get to valuable insights faster.
- Proven—leverages SBI’s third generation lentivector technology for optimal virus titers
- Validated—sequence-verified from LTR to LTR and expression-verified in HT-1080 cells
- Flexible—available in a range of fluorescent markers, selection markers, and promoters (Table 1)
While the pLL-EF1α-RFP-T2A-Blast Lenti-Labeler construct is compatible with most second and third generation packaging plasmid mixes, SBI recommends the use of pPACKH1 (Cat #LV500A-1) and TransDux MAX Transduction Reagent (Cat #LV860A-1) to achieve optimal virus titers and infection of target cells.
Find the right Lenti-Labeler construct for your studies
SBI offers Lenti-Labeler constructs with a range of selection markers, reporter genes, and two different promoters.
Table 1. Available Lenti-Labeler Constructs
Cat. # | Construct | Promoter | Reporter | Selection |
---|---|---|---|---|
LL100PA-1/LL100VA-1 | pLL-CMV-GFP-T2A-Puro | CMV | GFP | Puromycin |
LL105PA-1/LL105VA-1 | pLL-CMV-GFP-T2A-Blast | CMV | GFP | Blasticidin |
LL110PA-1/LL110VA-1 | pLL-CMV-RFP-T2A-Puro | CMV | RFP | Puromycin |
LL115PA-1/LL115VA-1 | pLL-CMV-RFP-T2A-Blast | CMV | RFP | Blasticidin |
LL120PA-1/LL120VA-1 | pLL-CMV-BFP-T2A-Puro | CMV | BFP | Puromycin |
LL125PA-1/LL125VA-1 | pLL-CMV-BFP-T2A-Blast | CMV | BFP | Blasticidin |
LL150PA-1/LL150VA-1 | pLL-CMV-Luciferase-T2A-Puro | CMV | Luciferase | Puromycin |
LL200PA-1/LL200VA-1 | pLL-EF1α-GFP-T2A-Puro | EF1α | GFP | Puromycin |
LL205PA-1/LL205VA-1 | pLL-EF1α-GFP-T2A-Blast | EF1α | GFP | Blasticidin |
LL210PA-1/LL210VA-1 | pLL-EF1α-RFP-T2A-Puro | EF1α | RFP | Puromycin |
LL215PA-1/LL215VA-1 | pLL-EF1α-RFP-T2A-Blast | EF1α | RFP | Blasticidin |
LL220PA-1/LL220VA-1 | pLL-EF1α-BFP-T2A-Puro | EF1α | BFP | Puromycin |
LL225PA-1/LL225VA-1 | pLL-EF1α-BFP-T2A-Blast | EF1α | BFP | Blasticidin |
LL250PA-1/LL250VA-1 | pLL-EF1α-Luciferase-T2A-Puro | EF1α | Luciferase | Puromycin |
LL300PA-1/LL300VA-1 | pLL-CMV-rFLuc-T2A-GFP | CMV | Luciferase & GFP | N/A |
LL310PA-1/LL310VA-1 | pLL-CMV-rFLuc-T2A-GFP-mPGK-Puro | CMV | Luciferase & GFP | Puromycin |
LL320PA-1/LL320VA-1 | pLL-CMV-rFLuc-T2A-mRFP-mPGK-Puro | CMV | Luciferase & RFP | Puromycin |
LL410PA-1/LL410VA-1 | pLL-EF1a-rFLuc-T2A-GFP-mPGK-Puro | EF1α | Luciferase & GFP | Puromycin |
LL420PA-1/LL420VA-1 | pLL-EF1a-rFLuc-T2A-mRFP-mPGK-Puro | EF1α | Luciferase & RFP | Puromycin |
References
How It Works
Supporting Data
FAQs
Resources
Citations
Related Products
Products
Catalog Number | Description | Size | Price | Quantity | Add to Cart | |||
---|---|---|---|---|---|---|---|---|
LL215PA-1 | pLL-EF1a-RFP-T2A-Blast (Lenti-Labeler™ plasmid) | 10 µg | $635 |
|
||||
LL215VA-1 | pLL-EF1a-RFP-T2A-Blast (Lenti-Labeler™ virus) | >2x10^6 IFUs | $635 |
|
Overview
Overview
Reliable cell labeling, delivered
SBI’s family of Lenti-Labeler™ constructs facilitate a wide range of studies—including cell tracking, high-throughput assays, and more—by enabling efficient and reliable labeling of your cells. The pLL-EF1α-RFP-T2A-Blast Lenti-Labeler™ construct expresses RFP from the EF1α promoter, which delivers moderate expression in most cell types (including primary and stem cells), and co-expresses the blasticidin resistance gene for selection in vitro prior to in vivo use. Available as either fully propagatable, sequence-verified plasmid DNA (Cat.# LL215PA-1) or ready-to-transduce pre-packaged lentivirus (Cat.# LL215VA-1), SBI’s pLL-EF1α-RFP-T2A-Blast Lenti-Labeler™ is designed for reliability, so you can get to valuable insights faster.
- Proven—leverages SBI’s third generation lentivector technology for optimal virus titers
- Validated—sequence-verified from LTR to LTR and expression-verified in HT-1080 cells
- Flexible—available in a range of fluorescent markers, selection markers, and promoters (Table 1)
While the pLL-EF1α-RFP-T2A-Blast Lenti-Labeler construct is compatible with most second and third generation packaging plasmid mixes, SBI recommends the use of pPACKH1 (Cat #LV500A-1) and TransDux MAX Transduction Reagent (Cat #LV860A-1) to achieve optimal virus titers and infection of target cells.
Find the right Lenti-Labeler construct for your studies
SBI offers Lenti-Labeler constructs with a range of selection markers, reporter genes, and two different promoters.
Table 1. Available Lenti-Labeler Constructs
Cat. # | Construct | Promoter | Reporter | Selection |
---|---|---|---|---|
LL100PA-1/LL100VA-1 | pLL-CMV-GFP-T2A-Puro | CMV | GFP | Puromycin |
LL105PA-1/LL105VA-1 | pLL-CMV-GFP-T2A-Blast | CMV | GFP | Blasticidin |
LL110PA-1/LL110VA-1 | pLL-CMV-RFP-T2A-Puro | CMV | RFP | Puromycin |
LL115PA-1/LL115VA-1 | pLL-CMV-RFP-T2A-Blast | CMV | RFP | Blasticidin |
LL120PA-1/LL120VA-1 | pLL-CMV-BFP-T2A-Puro | CMV | BFP | Puromycin |
LL125PA-1/LL125VA-1 | pLL-CMV-BFP-T2A-Blast | CMV | BFP | Blasticidin |
LL150PA-1/LL150VA-1 | pLL-CMV-Luciferase-T2A-Puro | CMV | Luciferase | Puromycin |
LL200PA-1/LL200VA-1 | pLL-EF1α-GFP-T2A-Puro | EF1α | GFP | Puromycin |
LL205PA-1/LL205VA-1 | pLL-EF1α-GFP-T2A-Blast | EF1α | GFP | Blasticidin |
LL210PA-1/LL210VA-1 | pLL-EF1α-RFP-T2A-Puro | EF1α | RFP | Puromycin |
LL215PA-1/LL215VA-1 | pLL-EF1α-RFP-T2A-Blast | EF1α | RFP | Blasticidin |
LL220PA-1/LL220VA-1 | pLL-EF1α-BFP-T2A-Puro | EF1α | BFP | Puromycin |
LL225PA-1/LL225VA-1 | pLL-EF1α-BFP-T2A-Blast | EF1α | BFP | Blasticidin |
LL250PA-1/LL250VA-1 | pLL-EF1α-Luciferase-T2A-Puro | EF1α | Luciferase | Puromycin |
LL300PA-1/LL300VA-1 | pLL-CMV-rFLuc-T2A-GFP | CMV | Luciferase & GFP | N/A |
LL310PA-1/LL310VA-1 | pLL-CMV-rFLuc-T2A-GFP-mPGK-Puro | CMV | Luciferase & GFP | Puromycin |
LL320PA-1/LL320VA-1 | pLL-CMV-rFLuc-T2A-mRFP-mPGK-Puro | CMV | Luciferase & RFP | Puromycin |
LL410PA-1/LL410VA-1 | pLL-EF1a-rFLuc-T2A-GFP-mPGK-Puro | EF1α | Luciferase & GFP | Puromycin |
LL420PA-1/LL420VA-1 | pLL-EF1a-rFLuc-T2A-mRFP-mPGK-Puro | EF1α | Luciferase & RFP | Puromycin |