pPACK-ID Integrase-defective Lentiviral Packaging Mix
- Safer—avoids the risks of insertional mutagenesis
- Controlled—transient expression in dividing cells, stable expression in quiescent cells
- Flexible—infect a wide range of cells with the VSV-G pseudotyped envelope
- Optimized—well-characterized virus production based on SBI’s proven pPACK system
- Ready-to-use—fully compatible with all third-generation lentiviral vectors and downstream virus concentration methods (PEG-it, ultracentrifugation, and ultrafiltration)
Products
Catalog Number | Description | Size | Price | Quantity | Add to Cart | |||
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LV520A-ID | pPACK-ID: Integrase-defective lentiviral packaging mix | 15 Reactions | $656 |
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LV525A-ID | pPACK-ID: Integrase-defective lentiviral packaging mix | 30 Reactions | $1097 |
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Overview
Overview
Robust, controlled transgene expression with a non-integrating lentiviral system
Combining all the advantages of lentiviral vectors with the benefits of episomal expression, SBI’s pPACK-ID Integrase-defective Lentiviral Packaging Mix is a great option for researchers who want to express transgenes in mammalian cells.
Based on SBI’s popular pPACKH1 lentiviral packaging system, pPACK-ID contains targeted mutations in the POL gene which codes for the integrase protein1. The result is a powerful lentiviral packaging system that is completely compatible with the full range of SBI’s lentiviral vectors, but without integration of the vector into the host genome. This enables transient gene expression in dividing cells and stable expression in quiescent or non-dividing cells. In addition, for researchers interested in gene therapy studies, this system supports transgene expression via lentiviral vectors without the potential risks of insertional mutagenesis.
- Safer—avoids the risks of insertional mutagenesis
- Controlled—transient expression in dividing cells, stable expression in quiescent cells
- Flexible—infect a wide range of cells with the VSV-G pseudotyped envelope
- Optimized—well-characterized virus production based on SBI’s proven pPACK system
- Ready-to-use—fully compatible with all third-generation lentiviral vectors and downstream virus concentration methods (PEG-it, ultracentrifugation, and ultrafiltration)
- The pPACK-ID-GAG plasmid contains the structural (Gag), and replication (Pol) genes which code for some of the proteins required to produce the lentivirus. It also encodes the viral Env gene, which encodes the envelope protein that defines the tropism (e. the range of infectable cells). The defective integrase is also located on this plasmid.
- The pPACK-ID-REV plasmid contains the regulatory protein Rev that is required for HIV replication.
- The pVSV-G plasmid expresses the envelope glycoprotein of vesicular stomatitis virus (VSV-G) from the CMV promoter. VSV-G pseudotyped viral particles mediate viral entry through lipid binding and plasma membrane fusion and can infect both mammalian and non-mammalian cells.
- Sengupta, et al. An Optimized Protocol for Packaging Pseudotyped Integrase Defective Lentivirus. Biol Proced Online. July 11, 2016. 18:14. PMCID: PMC4939624.
References
How It Works
Supporting Data
Supporting Data
pPACK-ID—the same great pPACK system but with episomal expression
pPACK-ID leverages the same great pPACK system that SBI customers have appreciated for years—easy-to-use, high-efficiency packaging resulting in high viral titers and robust transduction—but with no transgene integration.
With pPACK-ID, you still get great transduction efficiency (Figure 1, 2), but the non-integrating, episomal nature of the expression vector means that both the transgene (Figure 2) and transgene expression (Figure 3) are rapidly lost in dividing cells. In contrast, expression remains stable in quiescent and non-dividing cells (Figure 4).
FAQs
Resources
Citations
Related Products
Products
Catalog Number | Description | Size | Price | Quantity | Add to Cart | |||
---|---|---|---|---|---|---|---|---|
LV520A-ID | pPACK-ID: Integrase-defective lentiviral packaging mix | 15 Reactions | $656 |
|
||||
LV525A-ID | pPACK-ID: Integrase-defective lentiviral packaging mix | 30 Reactions | $1097 |
|
Overview
Overview
Robust, controlled transgene expression with a non-integrating lentiviral system
Combining all the advantages of lentiviral vectors with the benefits of episomal expression, SBI’s pPACK-ID Integrase-defective Lentiviral Packaging Mix is a great option for researchers who want to express transgenes in mammalian cells.
Based on SBI’s popular pPACKH1 lentiviral packaging system, pPACK-ID contains targeted mutations in the POL gene which codes for the integrase protein1. The result is a powerful lentiviral packaging system that is completely compatible with the full range of SBI’s lentiviral vectors, but without integration of the vector into the host genome. This enables transient gene expression in dividing cells and stable expression in quiescent or non-dividing cells. In addition, for researchers interested in gene therapy studies, this system supports transgene expression via lentiviral vectors without the potential risks of insertional mutagenesis.
- Safer—avoids the risks of insertional mutagenesis
- Controlled—transient expression in dividing cells, stable expression in quiescent cells
- Flexible—infect a wide range of cells with the VSV-G pseudotyped envelope
- Optimized—well-characterized virus production based on SBI’s proven pPACK system
- Ready-to-use—fully compatible with all third-generation lentiviral vectors and downstream virus concentration methods (PEG-it, ultracentrifugation, and ultrafiltration)
- The pPACK-ID-GAG plasmid contains the structural (Gag), and replication (Pol) genes which code for some of the proteins required to produce the lentivirus. It also encodes the viral Env gene, which encodes the envelope protein that defines the tropism (e. the range of infectable cells). The defective integrase is also located on this plasmid.
- The pPACK-ID-REV plasmid contains the regulatory protein Rev that is required for HIV replication.
- The pVSV-G plasmid expresses the envelope glycoprotein of vesicular stomatitis virus (VSV-G) from the CMV promoter. VSV-G pseudotyped viral particles mediate viral entry through lipid binding and plasma membrane fusion and can infect both mammalian and non-mammalian cells.
- Sengupta, et al. An Optimized Protocol for Packaging Pseudotyped Integrase Defective Lentivirus. Biol Proced Online. July 11, 2016. 18:14. PMCID: PMC4939624.
References
Supporting Data
Supporting Data
pPACK-ID—the same great pPACK system but with episomal expression
pPACK-ID leverages the same great pPACK system that SBI customers have appreciated for years—easy-to-use, high-efficiency packaging resulting in high viral titers and robust transduction—but with no transgene integration.
With pPACK-ID, you still get great transduction efficiency (Figure 1, 2), but the non-integrating, episomal nature of the expression vector means that both the transgene (Figure 2) and transgene expression (Figure 3) are rapidly lost in dividing cells. In contrast, expression remains stable in quiescent and non-dividing cells (Figure 4).