pPACK-SPIKE™ B.1.525 Variant Spike Protein Lentivector Packaging Mix
- Based on SBI’s popular and highly cited pPACKH1 Packaging System
- Uses codon-optimized SARS-CoV-2 “S” protein from variant B.1.525 in place of VSV-G envelope protein
- Spike protein mutations are ΔH69/V70, Q52R, E484K, D614G, Q677H, F888L
- Ideal for vaccine and antiviral efficacy studies under BSL2 conditions
- Package any 3rd-generation lentivector reporter of your choice, including SBI’s popular LentiLabeler reporters
Products
| Catalog Number | Description | Size | Price | Quantity | Add to Cart | |||
|---|---|---|---|---|---|---|---|---|
| CVD19-620A-1 | pPACK-SPIKE B.1.525, SARS-CoV-2 “S” Pseudotype – B.1.525 Variant – Lentivector Packaging Mix | 10 Reactions | $702 |
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| CVD19-625A-1 | pPACK-SPIKE B.1.525, SARS-CoV-2 “S” Pseudotype – B.1.525 Variant – Lentivector Packaging Mix | 25 Reactions | $1399 |
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| CVD19-629A-KIT | pPACK-SPIKE B.1.525 Combo Kit, includes Cat# CVD19-620A-1, plus PureFection Transfection Reagent (Cat# LV750A-1) and PEG-it Virus Concentration solution(Cat# LV810A-1) | 1 Kit | $1183 |
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Overview
Overview
Safely study the spike protein from emerging SARS-CoV-2 variants As potentially more transmissible variants of SARS-CoV-2 emerge, understanding how mutations in the spike protein impact SARS-CoV-2 behavior is critical. With the pPACK-SPIKE™ B.1.525 Variant Spike Protein Lentivector Packaging Mix, you can safely characterize the SARS-CoV-2 S1 spike protein from the emerging lineage B.1.525 [1], which contains the following mutations:- ΔH69/V70
- Q52R
- E484K
- D614G
- Q677H
- F888L
Designed to efficiently package most third-generation lentivectors, the pPACK-SPIKE B.1.525 Variant Spike Protein Lentivector Packaging Mix speeds and simplifies preparation of lentiviral particles pseudotyped with the SARS-CoV-2 ΔH69/V70, Q52R, E484K, D614G, Q677H, F888L spike glycoprotein, making it an ideal reagent for vaccine and antiviral drug discovery projects.
Based on SBI's highly cited pPACKH1 packaging system but with a truncated SARS-CoV-2 ΔH69/V70, Q52R, E484K, D614G, Q677H, F888L Spike protein [1] replacing the standard VSV-G envelope protein, the pPACK-SPIKE B.1.525 Variant Spike Protein Packaging Mix consists of three plasmids that produce all of the structural and replication proteins needed to transcribe and package an RNA copy of an expression lentivector into recombinant, ΔH69/V70, Q52R, E484K, D614G, Q677H, F888L “Spike” pseudotyped lentiviral particles. As a result, you can conduct a range of SARS-CoV-2 studies under BSL-2 conditions, including neutralization assays, studies of virus interactions with host surface proteins, and the development of vaccines and therapeutics.
For added convenience we also offer pPACK-BALD™, an envelope protein-free lentivector packaging mix that can be used as a negative control for any pPACK-SPIKE study, or for creating lentivirus particles pseudotyped with the envelope protein of your choice.
- Based on SBI’s popular and highly cited pPACKH1 Packaging System
- Uses codon-optimized SARS-CoV-2 “S” protein from variant B.1.525 in place of VSV-G envelope protein
- Spike protein mutations are ΔH69/V70, Q52R, E484K, D614G, Q677H, F888L
- Ideal for vaccine and antiviral efficacy studies under BSL2 conditions
- Package any 3rd-generation lentivector reporter of your choice, including SBI’s popular LentiLabeler reporters
- Compare with our full range of pPACK-SPIKE S protein pseudotyped lentiviruses to better understand emerging variants
- Use with pPACK-BALD™, an envelope protein-free lentivector packaging system that is an ideal negative control
pPACK-SPIKE B.1.525 Variant Spike Protein Lentivector Packaging Mix is available as stand-alone packaging mixes with sufficient plasmids for 10 reactions (standard size) or 25 reactions (XL), as well as in a convenient kit format that includes PureFection™ Transfection Reagent and PEG-it Virus Precipitation Solution.
See all of the available pPACK-SPIKE S protein variants:
| Catalog Number | Product Description | SPIKE PROTEIN MUTATION |
|---|---|---|
| CVD19-500A-1 | pPACK-SPIKE SARS-CoV-2 “S” Pseudotype Lentivector Packaging Mix | Original Wuhan-Hu-1 strain |
| CVD19-590A-1 | pPACK-SPIKE Alpha (B.1.1.7), SARS-CoV-2 "S" Pseudotype - Alpha (B.1.1.7, UK) Variant - Lentivector Packaging Mix | ΔH69/V70, ΔY144, N501Y, A570D, D614G, P681H, T716I, S982A, D1118H |
| CVD19-640A-1 | pPACK-SPIKE Beta (B.1.351), SARS-CoV-2 "S" Pseudotype - Beta (B.1.351) Variant - Lentivector Packaging Mix | L18F, D80A, D215G, Δ242-244, R246I, K417N, E484K, N501Y, D614G, A701V |
| CVD19-650A-1 | pPACK-SPIKE Delta (B.1.617.2), SARS-CoV-2 "S" Pseudotype - Delta (B.1.617.2) Variant - Lentivector Packaging Mix | T19R, G142D, ΔE156-157, R158G, L452R, T478K, D614G, P681R, D950N |
| CVD19-630A-1 | pPACK-SPIKE Gamma (P1), SARS-CoV-2 "S" Pseudotype - Gamma (P1) Variant - Lentivector Packaging Mix | L18F, T20N, P26S, D138Y, R190S, K417T, E484K, N501Y, D614G, H655Y, T1027I, V1176F |
| CVD19-660A-1 | pPACK-SPIKE Omicron (B.1.1.529), SARS-CoV-2 "S" Pseudotype - Omicron (B.1.1.529) Variant - Lentivector Packaging Mix | A67V, Δ69-70, T95I, Δ142-144, Y145D, Δ211, L212I, ins214EPE, G339D, S371L, S373P, S375F, K417N, N440K, G446S, S477N, T478K, E484A, Q493R, G496S,Q498R, N501Y, Y505H, T547K, D614G, H655Y, N679K, P681H, N764K, D796Y, N856K, Q954H, N969K, L981F |
| CVD19-530A-1 | pPACK-SPIKE D614G, SARS-CoV-2 “S” Pseudotype – D614G Mutant – Lentivector Packaging Mix | D614G |
| CVD19-560A-1 | pPACK-SPIKE N501Y, SARS-CoV-2 "S" Pseudotype - N501Y Mutant - Lentivector Packaging Mix | N501Y |
| CVD19-610A-1 | pPACK-SPIKE B.1.429, SARS-CoV-2 "S" Pseudotype - B.1.429 (CAL.20C) Variant - Lentivector Packaging Mix | S13I, W152C, L452R, & D614G |
| CVD19-620A-1 | pPACK-SPIKE B.1.525, SARS-CoV-2 "S" Pseudotype - B.1.525 Variant - Lentivector Packaging Mix | ΔH69/V70, Q52R, E484K, D614G, Q677H, & F888L |
| CVD19-580A-1 | pPACK-SPIKE B.1.351 RBD Mutations Lentivector Packaging Mix | K417N, E484K, & N501Y |
| CVD19-600A-1 | pPACK-SPIKE B.1.351 S1 Mutations (RBD+D614G) Lentivector Packaging Mix | K417N, E484K, N501Y, & D614G |
| LV550A-1 | pPACK-BALD™ (Negative Control Packaging Mix) | An envelope protein-free negative control for pPACK-SPIKE |
- O'Toole A, et al. pangolin: lineage assignment in an emerging pandemic as an epidemiological tool. Accessed April 10, 2021. https://cov-lineages.org/global_report_B.1.525.html
- Ou X, et al. Characterization of spike glycoprotein of SARS-CoV-2 on virus entry and its immune cross-reactivity with SARS-CoV. Nat. commun. 2020 Mar 27;11(1):1620. doi: 10.1038/s41467-020-15562-9. PMCID: PMC7100515.
References
How It Works
How It Works
pPACK-SPIKE is easy to use—simply co-transfect the pPACK-SPIKE Plasmids and your lentivector construct into 293TN producer cells or other lentiviral production cells, isolate pseudoviral particles with an optional concentration step, and conduct your studies.
Supporting Data
Supporting Data
To see data generated using the pPACK-SPIKE with the S protein from the original Wuhan-Hu-1 strain, visit the pPACK-SPIKE page.FAQs
Resources
Citations
Related Products
Products
| Catalog Number | Description | Size | Price | Quantity | Add to Cart | |||
|---|---|---|---|---|---|---|---|---|
| CVD19-620A-1 | pPACK-SPIKE B.1.525, SARS-CoV-2 “S” Pseudotype – B.1.525 Variant – Lentivector Packaging Mix | 10 Reactions | $702 |
|
||||
| CVD19-625A-1 | pPACK-SPIKE B.1.525, SARS-CoV-2 “S” Pseudotype – B.1.525 Variant – Lentivector Packaging Mix | 25 Reactions | $1399 |
|
||||
| CVD19-629A-KIT | pPACK-SPIKE B.1.525 Combo Kit, includes Cat# CVD19-620A-1, plus PureFection Transfection Reagent (Cat# LV750A-1) and PEG-it Virus Concentration solution(Cat# LV810A-1) | 1 Kit | $1183 |
|
||||
Overview
Overview
Safely study the spike protein from emerging SARS-CoV-2 variants As potentially more transmissible variants of SARS-CoV-2 emerge, understanding how mutations in the spike protein impact SARS-CoV-2 behavior is critical. With the pPACK-SPIKE™ B.1.525 Variant Spike Protein Lentivector Packaging Mix, you can safely characterize the SARS-CoV-2 S1 spike protein from the emerging lineage B.1.525 [1], which contains the following mutations:- ΔH69/V70
- Q52R
- E484K
- D614G
- Q677H
- F888L
Designed to efficiently package most third-generation lentivectors, the pPACK-SPIKE B.1.525 Variant Spike Protein Lentivector Packaging Mix speeds and simplifies preparation of lentiviral particles pseudotyped with the SARS-CoV-2 ΔH69/V70, Q52R, E484K, D614G, Q677H, F888L spike glycoprotein, making it an ideal reagent for vaccine and antiviral drug discovery projects.
Based on SBI's highly cited pPACKH1 packaging system but with a truncated SARS-CoV-2 ΔH69/V70, Q52R, E484K, D614G, Q677H, F888L Spike protein [1] replacing the standard VSV-G envelope protein, the pPACK-SPIKE B.1.525 Variant Spike Protein Packaging Mix consists of three plasmids that produce all of the structural and replication proteins needed to transcribe and package an RNA copy of an expression lentivector into recombinant, ΔH69/V70, Q52R, E484K, D614G, Q677H, F888L “Spike” pseudotyped lentiviral particles. As a result, you can conduct a range of SARS-CoV-2 studies under BSL-2 conditions, including neutralization assays, studies of virus interactions with host surface proteins, and the development of vaccines and therapeutics.
For added convenience we also offer pPACK-BALD™, an envelope protein-free lentivector packaging mix that can be used as a negative control for any pPACK-SPIKE study, or for creating lentivirus particles pseudotyped with the envelope protein of your choice.
- Based on SBI’s popular and highly cited pPACKH1 Packaging System
- Uses codon-optimized SARS-CoV-2 “S” protein from variant B.1.525 in place of VSV-G envelope protein
- Spike protein mutations are ΔH69/V70, Q52R, E484K, D614G, Q677H, F888L
- Ideal for vaccine and antiviral efficacy studies under BSL2 conditions
- Package any 3rd-generation lentivector reporter of your choice, including SBI’s popular LentiLabeler reporters
- Compare with our full range of pPACK-SPIKE S protein pseudotyped lentiviruses to better understand emerging variants
- Use with pPACK-BALD™, an envelope protein-free lentivector packaging system that is an ideal negative control
pPACK-SPIKE B.1.525 Variant Spike Protein Lentivector Packaging Mix is available as stand-alone packaging mixes with sufficient plasmids for 10 reactions (standard size) or 25 reactions (XL), as well as in a convenient kit format that includes PureFection™ Transfection Reagent and PEG-it Virus Precipitation Solution.
See all of the available pPACK-SPIKE S protein variants:
| Catalog Number | Product Description | SPIKE PROTEIN MUTATION |
|---|---|---|
| CVD19-500A-1 | pPACK-SPIKE SARS-CoV-2 “S” Pseudotype Lentivector Packaging Mix | Original Wuhan-Hu-1 strain |
| CVD19-590A-1 | pPACK-SPIKE Alpha (B.1.1.7), SARS-CoV-2 "S" Pseudotype - Alpha (B.1.1.7, UK) Variant - Lentivector Packaging Mix | ΔH69/V70, ΔY144, N501Y, A570D, D614G, P681H, T716I, S982A, D1118H |
| CVD19-640A-1 | pPACK-SPIKE Beta (B.1.351), SARS-CoV-2 "S" Pseudotype - Beta (B.1.351) Variant - Lentivector Packaging Mix | L18F, D80A, D215G, Δ242-244, R246I, K417N, E484K, N501Y, D614G, A701V |
| CVD19-650A-1 | pPACK-SPIKE Delta (B.1.617.2), SARS-CoV-2 "S" Pseudotype - Delta (B.1.617.2) Variant - Lentivector Packaging Mix | T19R, G142D, ΔE156-157, R158G, L452R, T478K, D614G, P681R, D950N |
| CVD19-630A-1 | pPACK-SPIKE Gamma (P1), SARS-CoV-2 "S" Pseudotype - Gamma (P1) Variant - Lentivector Packaging Mix | L18F, T20N, P26S, D138Y, R190S, K417T, E484K, N501Y, D614G, H655Y, T1027I, V1176F |
| CVD19-660A-1 | pPACK-SPIKE Omicron (B.1.1.529), SARS-CoV-2 "S" Pseudotype - Omicron (B.1.1.529) Variant - Lentivector Packaging Mix | A67V, Δ69-70, T95I, Δ142-144, Y145D, Δ211, L212I, ins214EPE, G339D, S371L, S373P, S375F, K417N, N440K, G446S, S477N, T478K, E484A, Q493R, G496S,Q498R, N501Y, Y505H, T547K, D614G, H655Y, N679K, P681H, N764K, D796Y, N856K, Q954H, N969K, L981F |
| CVD19-530A-1 | pPACK-SPIKE D614G, SARS-CoV-2 “S” Pseudotype – D614G Mutant – Lentivector Packaging Mix | D614G |
| CVD19-560A-1 | pPACK-SPIKE N501Y, SARS-CoV-2 "S" Pseudotype - N501Y Mutant - Lentivector Packaging Mix | N501Y |
| CVD19-610A-1 | pPACK-SPIKE B.1.429, SARS-CoV-2 "S" Pseudotype - B.1.429 (CAL.20C) Variant - Lentivector Packaging Mix | S13I, W152C, L452R, & D614G |
| CVD19-620A-1 | pPACK-SPIKE B.1.525, SARS-CoV-2 "S" Pseudotype - B.1.525 Variant - Lentivector Packaging Mix | ΔH69/V70, Q52R, E484K, D614G, Q677H, & F888L |
| CVD19-580A-1 | pPACK-SPIKE B.1.351 RBD Mutations Lentivector Packaging Mix | K417N, E484K, & N501Y |
| CVD19-600A-1 | pPACK-SPIKE B.1.351 S1 Mutations (RBD+D614G) Lentivector Packaging Mix | K417N, E484K, N501Y, & D614G |
| LV550A-1 | pPACK-BALD™ (Negative Control Packaging Mix) | An envelope protein-free negative control for pPACK-SPIKE |
- O'Toole A, et al. pangolin: lineage assignment in an emerging pandemic as an epidemiological tool. Accessed April 10, 2021. https://cov-lineages.org/global_report_B.1.525.html
- Ou X, et al. Characterization of spike glycoprotein of SARS-CoV-2 on virus entry and its immune cross-reactivity with SARS-CoV. Nat. commun. 2020 Mar 27;11(1):1620. doi: 10.1038/s41467-020-15562-9. PMCID: PMC7100515.
References
How It Works
How It Works
pPACK-SPIKE is easy to use—simply co-transfect the pPACK-SPIKE Plasmids and your lentivector construct into 293TN producer cells or other lentiviral production cells, isolate pseudoviral particles with an optional concentration step, and conduct your studies.