SeraMir Exosome RNA Amplification Kit for Media & Urine
- Reliable, reproducible exoRNA isolation from most biofluids
- Sensitive detection via exoRNA amplification
- Excellent preparation method for qPCR
- Can be used to amplify sense-strand exoRNAs for microarray construction
Products
Catalog Number | Description | Size | Price | Quantity | Add to Cart | |||
---|---|---|---|---|---|---|---|---|
RA800TC-1 | Complete SeraMir Exosome RNA Amplification kit from Media and Urine (10 mL ExoQuick-TC, 10 exoRNA columns, cDNA synthesis and amplification components) | 10 Reactions | $617 |
|
Overview
Overview
Profile exosomal RNA biomarkers and moreLooking for biomarkers? SBI’s SeraMir Exosome RNA Amplification Kit for Media & Urine can help. Providing everything you need to accurately and sensitively measure exosomal RNAs (exoRNAs) from most biofluids, our SeraMir Exosome RNA Amplification Kit for Media & Urine can take you from sample to amplified exoRNAs in a single day (for exoRNA isolation from serum, plasma, or ascites fluid, use the SeraMir Exosome RNA Amplification Kit for Media and Urine).
- Reliable, reproducible exoRNA isolation from most biofluids
- Sensitive detection via exoRNA amplification
- Excellent preparation method for qPCR
- Can be used to amplify sense-strand exoRNAs for microarray construction
With cargo that reflects the makeup of their parent cells and their easy isolation, researchers are increasingly turning to exosomes as a source of disease-related biomarkers. To simplify and standardize the isolation of RNA from exosomes, SBI has developed the SeraMir family of products.
The SeraMir Exosome RNA Amplification Kit for Media & Urine includes everything you need to accurately and sensitively measure RNAs from most biofluids—ExoQuick-TC for fast and efficient exosome isolation, a phenol-free lysis buffer and rapid spin columns for exoRNA isolation, and reagents for 3’ tailing and simultaneous tagging of both 5’ and 3’ ends during cDNA synthesis so you can go straight to qPCR.
In addition, primers for PCR amplification are included to make double-stranded cDNA via T7 IVT. These amplified exoRNAs can be used to construct microarrays or for next generation sequencing applications.
Choose the SeraMir Kit that’s right for youCat. # | Name | Kit includes | |||
---|---|---|---|---|---|
ExoQuick or ExoQuick-TC | SeraMir RNA columns and reagents | SeraMir cDNA synthesis and amplification reagents | 384-well plate miRNAs for human, mouse, or rat | ||
RA800A-1 | Complete SeraMir Exosome RNA Amplification Kit | ||||
RA800TC-1 | Complete SeraMir Exosome RNA Amplification Kit for Media and Urine | ||||
RA806A-1 | SeraMir Exosome RNA Purification Kit | ||||
RA806TC-1 | SeraMir Exosome RNA Purification Kit for Media and Urine | ||||
RA808A-1 | SeraMir Exosome RNA Purification Column Kit | ||||
RA820A-1 | Human Complete SeraMir Exosome RNA Amplification and Profiling Kit | ||||
RA820TC-1 | Human Complete SeraMir Exosome RNA Amplification and Profiling Kit for Media and Urine | ||||
RA821A-1 | Mouse Complete SeraMir Exosome RNA Amplification and Profiling Kit | ||||
RA821TC-1 | Mouse Complete SeraMir Exosome RNA Amplification and Profiling Kit for Media and Urine | ||||
RA822A-1 | Rat Complete SeraMir Exosome RNA Amplification and Profiling Kit | ||||
RA822TC-1 | Rat Complete SeraMir Exosome RNA Amplification and Profiling Kit for Media and Urine |
How It Works
How It Works
Go from sample to amplified exoRNAs in a single day
- Isolate exosomes from patient biofluids with the included ExoQuick-TC reagent
- Purify exoRNAs with SeraMir columns
- Tail and tag all exoRNAs for qPCR
- Perform second strand synthesis to generate cDNAs for amplification and T7 IVT
The amplified exoRNAs are ready for use in microarrays or NGS. Because RNA ligase is not used, you can avoid complicating adaptor concatemer artifacts.
Isolate serum exosomes and purify exoRNAs
Tail exoRNAs and synthesize double-tagged cDNA
Use the SeraMir spike-in RNA control in a qPCR assay to control for exoRNA recovery, tailing, and cDNA synthesis.
Supporting Data
FAQs
Resources
Related Products
Citations
-
Bakr, S, et al. (2018) Extracellular RNA Isolation from Cell Culture Supernatant. Methods Mol. Biol.. 2018; 1740:23-34. PM ID: 29388133
-
Nakano, T, et al. (2017) Hepatic miR-301a as a Liver Transplant Rejection Biomarker? And Its Role for Interleukin-6 Production in Hepatocytes. OMICS. 2017; 21(1):55-66. PM ID: 28271982
-
Peng, Y, Huleihel, L & Cardenes, N. (2017) Different MiroRNA Expression In MSC-Derived Exosomes: IPF Patients And Age-Matched Normal Individuals. Conference. 2017;. Link: Conference
-
Val, S, et al. (2017) Purification and characterization of microRNAs within middle ear fluid exosomes: implication in otitis media pathophysiology. Pediatr. Res.. 2017;. PM ID: 28157838
-
Zhou, X, et al. (2017) Characterization of mouse serum exosomal small RNA content: The origins and their roles in modulating inflammatory response. Oncotarget. 2017; 8(26):42712-42727. PM ID: 28514744
-
Holliday, LS, et al. (2017) Exosomes: novel regulators of bone remodelling and potential therapeutic agents for orthodontics. Orthod Craniofac Res. 2017;:95-99. PM ID: 28643924
-
Tsukita, S, et al. (2017) MicroRNAs 106b and 222 Improve Hyperglycemia in a Mouse Model of Insulin-Deficient Diabetes via Pancreatic β-Cell Proliferation. EBioMedicine. 2017; 15:163-172. PM ID: 27974246
-
Sueta, A, et al. (2017) Abstract P1-02-13: Differential expression of exosomal miRNAs between breast cancer patients with recurrence and no-recurrence. Abstract. 2017;. Link: Abstract
-
Selmaj, I, et al. (2017) Global Exosome Transcriptome Profiling Reveals Biomarkers for Multiple Sclerosis. Ann. Neurol.. 2017;. PM ID: 28411393
-
Koyama, S, et al. (2017) Dynamic changes of serum microRNA-122-5p through therapeutic courses indicates amelioration of acute liver injury accompanied by acute cardiac decompensation. ESC Heart Fail. 2017; 4(2):112-121. PM ID: 28451447
-
Hubal, MJ, et al. (2017) Circulating adipocyte-derived exosomal MicroRNAs associated with decreased insulin resistance after gastric bypass. Obesity (Silver Spring). 2017; 25(1):102-110. PM ID: 27883272
-
Faust, A, et al. (2017) Urinary bladder extracellular matrix hydrogels and matrix-bound vesicles differentially regulate central nervous system neuron viability and axon growth and branching. J Biomater Appl. 2017; 31(9):1277-1295. PM ID: 28447547
-
Zhang, R, et al. (2017) Serum long non coding RNA MALAT-1 protected by exosomes is up-regulated and promotes cell proliferation and migration in non-small cell lung cancer. Biochem. Biophys. Res. Commun.. 2017;. PM ID: 28623135
-
Huleihel, L, et al. (2017) Matrix bound nanovesicles recapitulate extracellular matrix effects on macrophage phenotype. Tissue Eng Part A. 2017;. PM ID: 28580875
-
Protocol, IIEI. (2017) List of Components. Product. 2017;. Link: Product
-
Josson, S, Gururajan, M & WKChung, L. (2017) miRNA Characterization from the Extracellular Vesicles. bio-protocol. 2017; 7(4). Link: bio-protocol
-
Gomez, D & Sempere, L. (2016) Characterization of Exosomal microRNAs in Pancreatic Cancer. Thesis. 2016;. Link: Thesis
-
Huang, Y, et al. (2016) 血清中外泌体及外泌体 RNA 提取方法的比较. 中华检验医学杂志. 2016; 39:427-432. Link: 中华检验医学杂志
-
Clayton, A, et al. (2016) The 2nd United Kingdom Extracellular Vesicle Forum Meeting Abstracts: 15 December 2015, Hadyn Ellis Building, Cardiff University.. J Extracell Vesicles. 2016; 5:30924. PM ID: 26928673
-
Crossland, RE, et al. (2016) Evaluation of optimal extracellular vesicle small RNA isolation and qRT-PCR normalisation for serum and urine. J. Immunol. Methods. 2016; 429:39-49. PM ID: 26723490
- See More
Products
Catalog Number | Description | Size | Price | Quantity | Add to Cart | |||
---|---|---|---|---|---|---|---|---|
RA800TC-1 | Complete SeraMir Exosome RNA Amplification kit from Media and Urine (10 mL ExoQuick-TC, 10 exoRNA columns, cDNA synthesis and amplification components) | 10 Reactions | $617 |
|
Overview
Overview
Profile exosomal RNA biomarkers and moreLooking for biomarkers? SBI’s SeraMir Exosome RNA Amplification Kit for Media & Urine can help. Providing everything you need to accurately and sensitively measure exosomal RNAs (exoRNAs) from most biofluids, our SeraMir Exosome RNA Amplification Kit for Media & Urine can take you from sample to amplified exoRNAs in a single day (for exoRNA isolation from serum, plasma, or ascites fluid, use the SeraMir Exosome RNA Amplification Kit for Media and Urine).
- Reliable, reproducible exoRNA isolation from most biofluids
- Sensitive detection via exoRNA amplification
- Excellent preparation method for qPCR
- Can be used to amplify sense-strand exoRNAs for microarray construction
With cargo that reflects the makeup of their parent cells and their easy isolation, researchers are increasingly turning to exosomes as a source of disease-related biomarkers. To simplify and standardize the isolation of RNA from exosomes, SBI has developed the SeraMir family of products.
The SeraMir Exosome RNA Amplification Kit for Media & Urine includes everything you need to accurately and sensitively measure RNAs from most biofluids—ExoQuick-TC for fast and efficient exosome isolation, a phenol-free lysis buffer and rapid spin columns for exoRNA isolation, and reagents for 3’ tailing and simultaneous tagging of both 5’ and 3’ ends during cDNA synthesis so you can go straight to qPCR.
In addition, primers for PCR amplification are included to make double-stranded cDNA via T7 IVT. These amplified exoRNAs can be used to construct microarrays or for next generation sequencing applications.
Choose the SeraMir Kit that’s right for youCat. # | Name | Kit includes | |||
---|---|---|---|---|---|
ExoQuick or ExoQuick-TC | SeraMir RNA columns and reagents | SeraMir cDNA synthesis and amplification reagents | 384-well plate miRNAs for human, mouse, or rat | ||
RA800A-1 | Complete SeraMir Exosome RNA Amplification Kit | ||||
RA800TC-1 | Complete SeraMir Exosome RNA Amplification Kit for Media and Urine | ||||
RA806A-1 | SeraMir Exosome RNA Purification Kit | ||||
RA806TC-1 | SeraMir Exosome RNA Purification Kit for Media and Urine | ||||
RA808A-1 | SeraMir Exosome RNA Purification Column Kit | ||||
RA820A-1 | Human Complete SeraMir Exosome RNA Amplification and Profiling Kit | ||||
RA820TC-1 | Human Complete SeraMir Exosome RNA Amplification and Profiling Kit for Media and Urine | ||||
RA821A-1 | Mouse Complete SeraMir Exosome RNA Amplification and Profiling Kit | ||||
RA821TC-1 | Mouse Complete SeraMir Exosome RNA Amplification and Profiling Kit for Media and Urine | ||||
RA822A-1 | Rat Complete SeraMir Exosome RNA Amplification and Profiling Kit | ||||
RA822TC-1 | Rat Complete SeraMir Exosome RNA Amplification and Profiling Kit for Media and Urine |
How It Works
How It Works
Go from sample to amplified exoRNAs in a single day
- Isolate exosomes from patient biofluids with the included ExoQuick-TC reagent
- Purify exoRNAs with SeraMir columns
- Tail and tag all exoRNAs for qPCR
- Perform second strand synthesis to generate cDNAs for amplification and T7 IVT
The amplified exoRNAs are ready for use in microarrays or NGS. Because RNA ligase is not used, you can avoid complicating adaptor concatemer artifacts.
Isolate serum exosomes and purify exoRNAs
Tail exoRNAs and synthesize double-tagged cDNA
Use the SeraMir spike-in RNA control in a qPCR assay to control for exoRNA recovery, tailing, and cDNA synthesis.
FAQs
Citations
-
Bakr, S, et al. (2018) Extracellular RNA Isolation from Cell Culture Supernatant. Methods Mol. Biol.. 2018; 1740:23-34. PM ID: 29388133
-
Nakano, T, et al. (2017) Hepatic miR-301a as a Liver Transplant Rejection Biomarker? And Its Role for Interleukin-6 Production in Hepatocytes. OMICS. 2017; 21(1):55-66. PM ID: 28271982
-
Peng, Y, Huleihel, L & Cardenes, N. (2017) Different MiroRNA Expression In MSC-Derived Exosomes: IPF Patients And Age-Matched Normal Individuals. Conference. 2017;. Link: Conference
-
Val, S, et al. (2017) Purification and characterization of microRNAs within middle ear fluid exosomes: implication in otitis media pathophysiology. Pediatr. Res.. 2017;. PM ID: 28157838
-
Zhou, X, et al. (2017) Characterization of mouse serum exosomal small RNA content: The origins and their roles in modulating inflammatory response. Oncotarget. 2017; 8(26):42712-42727. PM ID: 28514744
-
Holliday, LS, et al. (2017) Exosomes: novel regulators of bone remodelling and potential therapeutic agents for orthodontics. Orthod Craniofac Res. 2017;:95-99. PM ID: 28643924
-
Tsukita, S, et al. (2017) MicroRNAs 106b and 222 Improve Hyperglycemia in a Mouse Model of Insulin-Deficient Diabetes via Pancreatic β-Cell Proliferation. EBioMedicine. 2017; 15:163-172. PM ID: 27974246
-
Sueta, A, et al. (2017) Abstract P1-02-13: Differential expression of exosomal miRNAs between breast cancer patients with recurrence and no-recurrence. Abstract. 2017;. Link: Abstract
-
Selmaj, I, et al. (2017) Global Exosome Transcriptome Profiling Reveals Biomarkers for Multiple Sclerosis. Ann. Neurol.. 2017;. PM ID: 28411393
-
Koyama, S, et al. (2017) Dynamic changes of serum microRNA-122-5p through therapeutic courses indicates amelioration of acute liver injury accompanied by acute cardiac decompensation. ESC Heart Fail. 2017; 4(2):112-121. PM ID: 28451447
-
Hubal, MJ, et al. (2017) Circulating adipocyte-derived exosomal MicroRNAs associated with decreased insulin resistance after gastric bypass. Obesity (Silver Spring). 2017; 25(1):102-110. PM ID: 27883272
-
Faust, A, et al. (2017) Urinary bladder extracellular matrix hydrogels and matrix-bound vesicles differentially regulate central nervous system neuron viability and axon growth and branching. J Biomater Appl. 2017; 31(9):1277-1295. PM ID: 28447547
-
Zhang, R, et al. (2017) Serum long non coding RNA MALAT-1 protected by exosomes is up-regulated and promotes cell proliferation and migration in non-small cell lung cancer. Biochem. Biophys. Res. Commun.. 2017;. PM ID: 28623135
-
Huleihel, L, et al. (2017) Matrix bound nanovesicles recapitulate extracellular matrix effects on macrophage phenotype. Tissue Eng Part A. 2017;. PM ID: 28580875
-
Protocol, IIEI. (2017) List of Components. Product. 2017;. Link: Product
-
Josson, S, Gururajan, M & WKChung, L. (2017) miRNA Characterization from the Extracellular Vesicles. bio-protocol. 2017; 7(4). Link: bio-protocol
-
Gomez, D & Sempere, L. (2016) Characterization of Exosomal microRNAs in Pancreatic Cancer. Thesis. 2016;. Link: Thesis
-
Huang, Y, et al. (2016) 血清中外泌体及外泌体 RNA 提取方法的比较. 中华检验医学杂志. 2016; 39:427-432. Link: 中华检验医学杂志
-
Clayton, A, et al. (2016) The 2nd United Kingdom Extracellular Vesicle Forum Meeting Abstracts: 15 December 2015, Hadyn Ellis Building, Cardiff University.. J Extracell Vesicles. 2016; 5:30924. PM ID: 26928673
-
Crossland, RE, et al. (2016) Evaluation of optimal extracellular vesicle small RNA isolation and qRT-PCR normalisation for serum and urine. J. Immunol. Methods. 2016; 429:39-49. PM ID: 26723490
- See More