XStamp™ Pro anti-CD16 EV Targeting Kit

Engineer EVs to deliver cargo to cells expressing CD16 on their surface using the streamlined XStamp Pro anti-CD16 workflow—works with already-purified EVs

Description
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XStamp™ Pro anti-CD16 EV Targeting Kit
10 Reactions
XSTP910A-1
$ 695
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1-888-266-5066

Overview

Creating target-specific EVs just got a whole lot easier

The XStamp™ Pro anti-CD16 EV Targeting Kit enables streamlined generation of extracellular vesicles (EVs) specific for cells that express CD16 on their surface. Just decorate the outside of already isolated EVs with the XStamp Pro anti-CD16 ScFv and then isolate your target-specific EVs (see all of the targeting options in Table 1). Save time and simplify your EV engineering workflow with XStamp Pro.

While conventional methods for creating target-specific EVs are highly robust (see our XStamp Cloning and Expression Lentiector, Cat# XSTP710PA-1), they involve cloning and manipulation of the producer cells which can be challenging, especially if the cells are hard to transfect. To streamline this process and help researchers more quickly and easily create EVs that preferentially localize at a specific target, SBI turned to an exciting new technology, XStamp Pro, which works on already purified EVs—no cloning or transfection required. Simply incubate purified EVs with the XStamp Pro ScFv for one-hour and then isolate your target-specific EVs.

  • Speed production of target-specific EVs with the next generation of XStamp technology
  • Streamline engineering by working directly with purified EVs—no expression vector or transfection required
  • Save hands-on time with a simple workflow—just incubate EVs with the XStamp ScFv for one hour and isolate target-specific EVs
  • Easily generate multiple targeting variants for quick screening studies
  • Compatible with any EV loading protocol

Each XStamp Pro anti-CD16 EV Targeting Kit comes with enough XStamp Pro anti-CD16 ScFv for 10 reactions* and ExoQuick-TC for EV isolation. Once the reaction is complete and your target-specific EVs are isolated, they are ready to use for cargo delivery.

For even faster workflows, you can use Exo-Fect siRNA/miRNA EV Transfection Reagent (Cat.# EXFT200A-1) or the original Exo-Fect Reagent (Cat.# EXFT10A-1 /20A-1) to load cargo into isolated EVs before adding the targeting specificity with XStamp Pro.

Cat. #ProductSpecificity
XSTP900A-1XStamp Pro StreptavidinAny - determined by user-supplied biotinylated moiety
XSTP905A-1XStamp Pro anti-HIVHIV-infected cells
XSTP910A-1XStamp Pro anti-CD16CD16-expressing cells
XSTP915A-1XStamp Pro anti-PD-1PD-1-epxressing cells

*One reaction can label up to 300 µg protein equivalent of EVs.

How It Works

Engineer target specificity into already-purified EVs

XStamp Pro takes advantage of the same EV surface-directing protein used in our original XStamp products but skips the expression vector and the transfection step for a faster, simpler workflow. You get an already purified peptide consisting of the target-specific ScFv fused to the EV surface-directing protein—the XStamp Pro ScFv. Upon incubation with isolated EVs, the XStamp Pro ScFv self-inserts into EV membranes creating your target-specific EVs (Figure 1). Incubation is complete in 1 hour, after which the target-specific EVs can be isolated using the included ExoQuick-TC reagent or other isolation method of your choice, and then used to deliver cargo.

The XStamp Pro anti-CD16 workflow

Figure 1. The streamlined XStamp Pro Streptavidin workflow.

 

Supporting Data

See how effective XStamp Pro anti-CD16 scFv is at delivering cargo

Target-specific EVs engineered using XStamp Pro anti-CD16 are highly specific and efficiently internalized

 

Figure 2. Target-specific EVs engineered using XStamp Pro anti-CD16 are highly specific and efficiently internalized. We stimulated human peripheral blood mononuclear cells (PBMCs) with IL-10 for 60 hours, and then added EVs coated with XStamp Pro anti-CD16 ScFv. These EVs were derived from human monocytes and loaded with a Cy5-labeled miRNA. After 15 hours, the cells were fixed, stained, and analyzed on a flow cytometer. Only CD16+ cells had internalized the EVs (47.9% of CD16+ cells compared to 0.772% of CD16− cells) demonstrating the specificity of XStamp Pro anti-CD16. Data courtesy of Lynn Pulliam, UCSF. 


Product Documentation