XStamp™ Pro anti-PD-1 EV Targeting Kit

Engineer EVs to deliver cargo to cells expressing PD-1 on their surface using the streamlined XStamp Pro PD-1 workflow—works with already-purified EVs

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XStamp™ Pro anti-PD-1 EV Targeting Kit
10 Reactions
XSTP915A-1
$ 695
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1-888-266-5066

Overview

Creating target-specific EVs just got a whole lot easier

The XStamp™ Pro anti-PD-1 EV Targeting Kit enables streamlined generation of extracellular vesicles (EVs) specific for cells that express PD-1 on their surface. Just decorate the outside of already isolated EVs with the XStamp Pro anti-PD-1 ScFv and then isolated your targeting EVs (see all of the targeting options in Table 1). Save time and simplify your EV engineering workflow with XStamp Pro.

While conventional methods for creating target-specific EVs are highly robust (see our XStamp Cloning and Expression Lentiector, Cat# XSTP710PA-1), they involve cloning and manipulation of the producer cells which can be challenging, especially if the cells are hard to transfect. To streamline this process and help researchers more quickly and easily create EVs that preferentially localize at a specific target, SBI turned to an exciting new technology, XStamp Pro, which works on already purified EVs—no cloning or transfection required. Simply incubate purified EVs with the XStamp Pro ScFv for one-hour and then isolate your target-specific EVs.

  • Speed production of target-specific EVs with the next generation of XStamp technology
  • Streamline engineering by working directly with purified EVs—no expression vector or transfection required
  • Save hands-on time with a simple workflow—just incubate EVs with the XStamp ScFv for one hour and isolate target-specific EVs
  • Easily generate multiple targeting variants for quick screening studies
  • Compatible with any EV loading protocol

Each XStamp Pro anti-CD16 EV Targeting Kit comes with enough XStamp Pro anti-CD16 ScFv for 10 reactions* and ExoQuick-TC for EV isolation. Once the reaction is complete and your target-specific EVs are isolated, they are ready to use for cargo delivery.

For even faster workflows, you can use Exo-Fect siRNA/miRNA EV Transfection Reagent (Cat.# EXFT200A-1) or the original Exo-Fect Reagent (Cat.# EXFT10A-1 /20A-1) to load cargo into isolated EVs before adding the targeting specificity with XStamp Pro.

Cat. #ProductSpecificity
XSTP900A-1XStamp Pro StreptavidinAny - determined by user-supplied biotinylated moiety
XSTP905A-1XStamp Pro anti-HIVHIV-infected cells
XSTP910A-1XStamp Pro anti-CD16CD16-expressing cells
XSTP915A-1XStamp Pro anti-PD-1PD-1-epxressing cells

*One reaction can label up to 300 µg protein equivalent of EVs.

How It Works

Engineer target specificity into already-purified EVs

XStamp Pro takes advantage of the same EV surface-directing protein used in our original XStamp products but skips the expression vector and the transfection step for a faster, simpler workflow. You get an already purified peptide consisting of the target-specific ScFv fused to the EV surface-directing protein—the XStamp Pro ScFv. Upon incubation with isolated EVs, the XStamp Pro ScFv self-inserts into EV membranes creating your target-specific EVs (Figure 1). Incubation is complete in 1 hour, after which the target-specific EVs can be isolated using the included ExoQuick-TC reagent or other isolation method of your choice, and then used to deliver cargo.

The XStamp Pro anti-PD-1 workflow

Figure 1. The streamlined XStamp Pro Streptavidin workflow.

 

Supporting Data

See how effective XStamp Pro anti-PD-1 scFv is at delivering cargo

 

Target-specific EVs engineered using XStamp Pro are highly specific

Target-specific EVs engineered using XStamp Pro are highly specific

Figure 2. Target-specific EVs engineered using XStamp Pro are highly specific. (A) We labeled serum-derived EVs with SBI’s ExoFlow-ONE Topaz Blue flow cytometry dye (Cat.# EXOF400A-1) and then coated them with either XStamp Pro anti-HIV ScFv (mistargeted control) or XStamp Pro anti-PD-1 ScFv. These EVs were incubated with activated CD4+ T cells for 36 hours, the cells stained for PD-1, and then analyzed by flow cytometry. Only XStamp Pro anti-PD-1 EVs are internalized by PD-1-expressing cells (compare bottom right panel to bottom left panel, blue arrow). (B) A comparison of the absolute cell count from each condition further demonstrates the specificity of the XStamp Pro anti-PD-1 EVs as the mistargeted EVs (decorated with XStamp Pro anti-HIV ScFv) were not taken up by the activated cells. Data courtesy of Pooja Bhardwaj and Satish Pillai, UCSF. 

 


Product Documentation