Creating target-specific EVs just got a whole lot easier
The XStamp™ Pro anti-PD-1 EV Targeting Kit enables streamlined generation of extracellular vesicles (EVs) specific for cells that express PD-1 on their surface. Just decorate the outside of already isolated EVs with the XStamp Pro anti-PD-1 ScFv and then isolated your targeting EVs (see all of the targeting options in Table 1). Save time and simplify your EV engineering workflow with XStamp Pro.
While conventional methods for creating target-specific EVs are highly robust (see our XStamp Cloning and Expression Lentiector, Cat# XSTP710PA-1), they involve cloning and manipulation of the producer cells which can be challenging, especially if the cells are hard to transfect. To streamline this process and help researchers more quickly and easily create EVs that preferentially localize at a specific target, SBI turned to an exciting new technology, XStamp Pro, which works on already purified EVs—no cloning or transfection required. Simply incubate purified EVs with the XStamp Pro ScFv for one-hour and then isolate your target-specific EVs.
- Speed production of target-specific EVs with the next generation of XStamp technology
- Streamline engineering by working directly with purified EVs—no expression vector or transfection required
- Save hands-on time with a simple workflow—just incubate EVs with the XStamp ScFv for one hour and isolate target-specific EVs
- Easily generate multiple targeting variants for quick screening studies
- Compatible with any EV loading protocol
Each XStamp Pro anti-CD16 EV Targeting Kit comes with enough XStamp Pro anti-CD16 ScFv for 10 reactions* and ExoQuick-TC for EV isolation. Once the reaction is complete and your target-specific EVs are isolated, they are ready to use for cargo delivery.
For even faster workflows, you can use Exo-Fect siRNA/miRNA EV Transfection Reagent (Cat.# EXFT200A-1) or the original Exo-Fect Reagent (Cat.# EXFT10A-1 /20A-1) to load cargo into isolated EVs before adding the targeting specificity with XStamp Pro.
|XSTP900A-1||XStamp Pro Streptavidin||Any - determined by user-supplied biotinylated moiety|
|XSTP905A-1||XStamp Pro anti-HIV||HIV-infected cells|
|XSTP910A-1||XStamp Pro anti-CD16||CD16-expressing cells|
|XSTP915A-1||XStamp Pro anti-PD-1||PD-1-epxressing cells|
*One reaction can label up to 300 µg protein equivalent of EVs.