Exo-Flow96 Tetra IP Kit
- Selection of well-validated antibodies for reliable and reproducible purification
- Large-sized magnetic beads increase the efficiency of exosome capture
- Available in 32- and 96-well formats for high-throughput, affinity-based exosome isolation
Products
Catalog Number | Description | Size | Price | Quantity | Add to Cart | |||
---|---|---|---|---|---|---|---|---|
EXOFLOW96A-Tetra | Exo-Flow96 Tetra IP kit (CD9, CD63, CD81) | 96 Reactions | $1611 |
|
Overview
Overview
Streamline affinity-based exosome immunopurification
With magnetic beads already pre-coupled to antibodies to the tetraspanin proteins CD63, CD81, and CD9, and delivered in a 96-well format, SBI’s Exo-Flow96 Tetra IP Kit simplifies high-throughput, antibody-based exosome isolation. Our magnetic bead-coupled anti-CD9 antibodies are extensively validated, and the high-quality Exo-Flow IP kit components ensure reliable, reproducible affinity-based exosome purification directly from serum or plasma. Exosomes can also be purified from other biofluids such as media, urine, and CSF, but must first be concentrated using either ExoQuick-TC® or ultracentrifugation.
- Selection of well-validated antibodies for reliable and reproducible purification
- Large-sized magnetic beads increase the efficiency of exosome capture
- Available in 32- and 96-well formats for high-throughput, affinity-based exosome isolation
Even better, with our larger-than-typical bead size (9.1 μm diameter) exosome capture is highly efficient, enabling capture of billions of exosomes from one sample.
![Exosome capture is highly efficient with large bead size](/wp-content/uploads/exosome-research/exo-flow-beads.png)
Each IP kit comes with all the necessary reagents for immunopurification—capture antibody pre-coupled to magnetic beads in 96-well clear plates (as 8-well strips) with covers, wash buffer, Exosome Elution Buffer, and clearing reagent (to remove Exosome Elution Buffer). A 96-well plate magnet, the Exo-FlowMag96, can be purchased separately (Cat.# EXOFLOWMAG-1).
![The optional Exo-FlowMag96 simplifies Exo-Flow IP Kit isolations](/wp-content/uploads/exosome-research/exo-flow32-exo-flow96-bead-capture.jpg)
Different formats for different needs
To facilitate a range of studies, SBI built the Exo-Flow IP system to be highly modular. We offer a range of magnetic bead-coupled antibodies in 32- and 96-well formats.
Cat.# | Kit |
---|---|
EXOFLOW32A-CD63 | Exo-Flow32 CD63 IP kit |
EXOFLOW32A-CD81 | Exo-Flow32 CD81 IP kit |
EXOFLOW32A-CD9 | Exo-Flow32 CD9 IP kit |
EXOFLOW32A-Tetra | Exo-Flow32 Tetra IP kit (CD9, CD63, CD81) |
EXOFLOW96A-CD63 | Exo-Flow96 CD63 IP kit |
EXOFLOW96A-CD81 | Exo-Flow96 CD81 IP kit |
EXOFLOW96A-CD9 | Exo-Flow96 CD9 IP kit |
EXOFLOW96A-Tetra | Exo-Flow96 Tetra IP kit (CD9, CD63, CD81) |
References
How It Works
Supporting Data
Supporting Data
See isolation data using Exo-FLOW IP Kits
For these studies, either human serum or HEK293 exosomes concentrated from cell culture media using ExoQuick-TC were added to the antibody-coupled magnetic beads. After washing, exosomes were eluted and recovery estimated using a standard BCA protein assay.
Figure 1. CD63 and CD9, two exosome markers, are readily detected in samples purified using Exo-Flow Kits. Approximately 1 µg of protein was loaded per well on a 4–20% gradient protein PAGE. The proteins were separated and transferred to nitrocellulose membranes for Western blot analysis. The blots were probed with either with anti-CD63 or anti-CD9 antibodies to detect the exosome protein markers.
Figure 2. NanoSight analysis shows Exo-Flow IP Kits deliver good yields of particles whose sizes are consistent with exosomes.
FAQs
Related Products
Resources
Citations
-
Boyer, E, et al. (2024) Comparison of plasma soluble and extracellular vesicles-associated biomarkers in Alzheimer’s disease patients and cognitively normal individuals. Alzheimer's research & therapy. 2024; 16(1):141. PM ID: 38943196
-
Kim, H & Kim, B. (2024) Osteogenic and Protective Effects of Erucic Acid and Erucic Acid-Induced Exosomes against Fine Dust. Preprint. 2024;. Link: Preprint
-
Karpurapu, M, et al. (2024) The calcineurin-NFATc pathway modulates the lipid mediators in BAL fluid extracellular vesicles, thereby regulating microvascular endothelial cell barrier function. Frontiers in physiology. 2024; 15:1378565. PM ID: 38812883
-
Siametis, A, et al. (2024) Transcription stress at telomeres leads to cytosolic DNA release and paracrine senescence. Nature communications. 2024; 15(1):4061. PM ID: 38744897
-
Boyer, E, et al. (2024) Comparison of plasma soluble and extracellular vesicles-associated biomarkers in Alzheimer’s Disease patients and cognitively normal individuals. medRxiv. 2024;. Link: medRxiv
-
Klemetti, MM, et al. (2024) Lipid profile of circulating placental extracellular vesicles during pregnancy identifies foetal growth restriction risk. Journal of extracellular vesicles. 2024; 13(2):e12413. PM ID: 38353485
-
Mekala, N, et al. (2024) Alcohol and e-cigarette damage alveolar-epithelial barrier by activation of P2X7r and provoke brain endothelial injury via extracellular vesicles. Cell communication and signaling : CCS. 2024; 22(1):39. PM ID: 38225580
-
Yuan, X, et al. (2023) Contribution of Hepatic Steatosis-Intensified Extracellular Vesicle Release to Aggravated Inflammatory Endothelial Injury in Liver-Specific Asah1 Gene Knockout Mice. The American journal of pathology. 2023;. PM ID: 36638912
-
Shen, H & Lane, RA. (2023) Extracellular Vesicles from Inflammation-Primed Adipose-Derived Stem Cells Enhance Achilles Tendon Repair by Reducing Inflammation and Promoting Intrinsic Healing. bioRxiv : the preprint server for biology. 2023;. PM ID: 36778262
-
Bertolini, I, et al. (2023) Intercellular HIF1a reprogams mammary progenitors and myeloid immune evasion to drive high-risk breast lesions. The Journal of clinical investigation. 2023;. PM ID: 36892943
-
Mimmi, S, et al. (2023) SARS CoV-2 spike protein-guided exosome isolation facilitates detection of potential miRNA biomarkers in COVID-19 infections. Clinical chemistry and laboratory medicine. 2023;. PM ID: 36972680
-
Guda, P, et al. (2023) Nanoscopic and functional characterization of keratinocyte-originating exosomes in the wound fluid of non-diabetic and diabetic chronic wound patients. Nano Today. 2023; 52:101954. Link: Nano Today
-
Garbin, A, et al. (2023) MiR-146a-5p enrichment in small-extracellular vesicles of relapsed pediatric ALCL patients promotes macrophages infiltration and differentiation. Biochemical pharmacology. 2023; 215:115747. PM ID: 37591448
-
Nakazaki, M, et al. (2023) Human mesenchymal stem-derived extracellular vesicles improve body growth and motor function following severe spinal cord injury in rat. Clinical and translational medicine. 2023; 13(6):e1284. PM ID: 37323108
-
Nirujogi, TS, et al. (2022) Lipidomic Profiling of Bronchoalveolar Lavage Fluid Extracellular Vesicles Indicates Their Involvement in Lipopolysaccharide-Induced Acute Lung Injury. Journal of innate immunity. 2022;:1-14. PM ID: 35367992
-
Luo, Z, et al. (2022) Human bone marrow mesenchymal stem cell-derived extracellular vesicles inhibit shoulder stiffness via let-7a/Tgfbr1 axis. Bioactive Materials. 2022;. Link: Bioactive Materials
-
Marra, KV, et al. (2022) Bioactive extracellular vesicles from a subset of endothelial progenitor cells rescue retinal ischemia and neurodegeneration. JCI insight. 2022; 7(12). PM ID: 35639473
-
Li, Y, et al. (2022) Exosome-shuttled miR-126 mediates ethanol-induced disruption of neural crest cell-placode cell interaction by targeting SDF1. Research Square. 2022;. Link: Research Square
-
Koken, GY, et al. (2022) Wharton Jelly Derived Mesenchymal Stem Cell’s Exosomes Demonstrate Significant Antileishmanial and Wound Healing Effects in Combination with Aloe-Emodin: An in Vitro Study. Journal of pharmaceutical sciences. 2022;. PM ID: 35995206
-
Mullen, M, et al. (2022) Mechanical strain drives exosome production, function, and miRNA cargo in C2C12 muscle progenitor cells. Journal of orthopaedic research : official publication of the Orthopaedic Research Society. 2022;. PM ID: 36250617
- See More
BizGenius Citations
Products
Catalog Number | Description | Size | Price | Quantity | Add to Cart | |||
---|---|---|---|---|---|---|---|---|
EXOFLOW96A-Tetra | Exo-Flow96 Tetra IP kit (CD9, CD63, CD81) | 96 Reactions | $1611 |
|
Overview
Overview
Streamline affinity-based exosome immunopurification
With magnetic beads already pre-coupled to antibodies to the tetraspanin proteins CD63, CD81, and CD9, and delivered in a 96-well format, SBI’s Exo-Flow96 Tetra IP Kit simplifies high-throughput, antibody-based exosome isolation. Our magnetic bead-coupled anti-CD9 antibodies are extensively validated, and the high-quality Exo-Flow IP kit components ensure reliable, reproducible affinity-based exosome purification directly from serum or plasma. Exosomes can also be purified from other biofluids such as media, urine, and CSF, but must first be concentrated using either ExoQuick-TC® or ultracentrifugation.
- Selection of well-validated antibodies for reliable and reproducible purification
- Large-sized magnetic beads increase the efficiency of exosome capture
- Available in 32- and 96-well formats for high-throughput, affinity-based exosome isolation
Even better, with our larger-than-typical bead size (9.1 μm diameter) exosome capture is highly efficient, enabling capture of billions of exosomes from one sample.
![Exosome capture is highly efficient with large bead size](/wp-content/uploads/exosome-research/exo-flow-beads.png)
Each IP kit comes with all the necessary reagents for immunopurification—capture antibody pre-coupled to magnetic beads in 96-well clear plates (as 8-well strips) with covers, wash buffer, Exosome Elution Buffer, and clearing reagent (to remove Exosome Elution Buffer). A 96-well plate magnet, the Exo-FlowMag96, can be purchased separately (Cat.# EXOFLOWMAG-1).
![The optional Exo-FlowMag96 simplifies Exo-Flow IP Kit isolations](/wp-content/uploads/exosome-research/exo-flow32-exo-flow96-bead-capture.jpg)
Different formats for different needs
To facilitate a range of studies, SBI built the Exo-Flow IP system to be highly modular. We offer a range of magnetic bead-coupled antibodies in 32- and 96-well formats.
Cat.# | Kit |
---|---|
EXOFLOW32A-CD63 | Exo-Flow32 CD63 IP kit |
EXOFLOW32A-CD81 | Exo-Flow32 CD81 IP kit |
EXOFLOW32A-CD9 | Exo-Flow32 CD9 IP kit |
EXOFLOW32A-Tetra | Exo-Flow32 Tetra IP kit (CD9, CD63, CD81) |
EXOFLOW96A-CD63 | Exo-Flow96 CD63 IP kit |
EXOFLOW96A-CD81 | Exo-Flow96 CD81 IP kit |
EXOFLOW96A-CD9 | Exo-Flow96 CD9 IP kit |
EXOFLOW96A-Tetra | Exo-Flow96 Tetra IP kit (CD9, CD63, CD81) |
References
How It Works
Supporting Data
Supporting Data
See isolation data using Exo-FLOW IP Kits
For these studies, either human serum or HEK293 exosomes concentrated from cell culture media using ExoQuick-TC were added to the antibody-coupled magnetic beads. After washing, exosomes were eluted and recovery estimated using a standard BCA protein assay.
Figure 1. CD63 and CD9, two exosome markers, are readily detected in samples purified using Exo-Flow Kits. Approximately 1 µg of protein was loaded per well on a 4–20% gradient protein PAGE. The proteins were separated and transferred to nitrocellulose membranes for Western blot analysis. The blots were probed with either with anti-CD63 or anti-CD9 antibodies to detect the exosome protein markers.
Figure 2. NanoSight analysis shows Exo-Flow IP Kits deliver good yields of particles whose sizes are consistent with exosomes.
FAQs
Citations
-
Boyer, E, et al. (2024) Comparison of plasma soluble and extracellular vesicles-associated biomarkers in Alzheimer’s disease patients and cognitively normal individuals. Alzheimer's research & therapy. 2024; 16(1):141. PM ID: 38943196
-
Kim, H & Kim, B. (2024) Osteogenic and Protective Effects of Erucic Acid and Erucic Acid-Induced Exosomes against Fine Dust. Preprint. 2024;. Link: Preprint
-
Karpurapu, M, et al. (2024) The calcineurin-NFATc pathway modulates the lipid mediators in BAL fluid extracellular vesicles, thereby regulating microvascular endothelial cell barrier function. Frontiers in physiology. 2024; 15:1378565. PM ID: 38812883
-
Siametis, A, et al. (2024) Transcription stress at telomeres leads to cytosolic DNA release and paracrine senescence. Nature communications. 2024; 15(1):4061. PM ID: 38744897
-
Boyer, E, et al. (2024) Comparison of plasma soluble and extracellular vesicles-associated biomarkers in Alzheimer’s Disease patients and cognitively normal individuals. medRxiv. 2024;. Link: medRxiv
-
Klemetti, MM, et al. (2024) Lipid profile of circulating placental extracellular vesicles during pregnancy identifies foetal growth restriction risk. Journal of extracellular vesicles. 2024; 13(2):e12413. PM ID: 38353485
-
Mekala, N, et al. (2024) Alcohol and e-cigarette damage alveolar-epithelial barrier by activation of P2X7r and provoke brain endothelial injury via extracellular vesicles. Cell communication and signaling : CCS. 2024; 22(1):39. PM ID: 38225580
-
Yuan, X, et al. (2023) Contribution of Hepatic Steatosis-Intensified Extracellular Vesicle Release to Aggravated Inflammatory Endothelial Injury in Liver-Specific Asah1 Gene Knockout Mice. The American journal of pathology. 2023;. PM ID: 36638912
-
Shen, H & Lane, RA. (2023) Extracellular Vesicles from Inflammation-Primed Adipose-Derived Stem Cells Enhance Achilles Tendon Repair by Reducing Inflammation and Promoting Intrinsic Healing. bioRxiv : the preprint server for biology. 2023;. PM ID: 36778262
-
Bertolini, I, et al. (2023) Intercellular HIF1a reprogams mammary progenitors and myeloid immune evasion to drive high-risk breast lesions. The Journal of clinical investigation. 2023;. PM ID: 36892943
-
Mimmi, S, et al. (2023) SARS CoV-2 spike protein-guided exosome isolation facilitates detection of potential miRNA biomarkers in COVID-19 infections. Clinical chemistry and laboratory medicine. 2023;. PM ID: 36972680
-
Guda, P, et al. (2023) Nanoscopic and functional characterization of keratinocyte-originating exosomes in the wound fluid of non-diabetic and diabetic chronic wound patients. Nano Today. 2023; 52:101954. Link: Nano Today
-
Garbin, A, et al. (2023) MiR-146a-5p enrichment in small-extracellular vesicles of relapsed pediatric ALCL patients promotes macrophages infiltration and differentiation. Biochemical pharmacology. 2023; 215:115747. PM ID: 37591448
-
Nakazaki, M, et al. (2023) Human mesenchymal stem-derived extracellular vesicles improve body growth and motor function following severe spinal cord injury in rat. Clinical and translational medicine. 2023; 13(6):e1284. PM ID: 37323108
-
Nirujogi, TS, et al. (2022) Lipidomic Profiling of Bronchoalveolar Lavage Fluid Extracellular Vesicles Indicates Their Involvement in Lipopolysaccharide-Induced Acute Lung Injury. Journal of innate immunity. 2022;:1-14. PM ID: 35367992
-
Luo, Z, et al. (2022) Human bone marrow mesenchymal stem cell-derived extracellular vesicles inhibit shoulder stiffness via let-7a/Tgfbr1 axis. Bioactive Materials. 2022;. Link: Bioactive Materials
-
Marra, KV, et al. (2022) Bioactive extracellular vesicles from a subset of endothelial progenitor cells rescue retinal ischemia and neurodegeneration. JCI insight. 2022; 7(12). PM ID: 35639473
-
Li, Y, et al. (2022) Exosome-shuttled miR-126 mediates ethanol-induced disruption of neural crest cell-placode cell interaction by targeting SDF1. Research Square. 2022;. Link: Research Square
-
Koken, GY, et al. (2022) Wharton Jelly Derived Mesenchymal Stem Cell’s Exosomes Demonstrate Significant Antileishmanial and Wound Healing Effects in Combination with Aloe-Emodin: An in Vitro Study. Journal of pharmaceutical sciences. 2022;. PM ID: 35995206
-
Mullen, M, et al. (2022) Mechanical strain drives exosome production, function, and miRNA cargo in C2C12 muscle progenitor cells. Journal of orthopaedic research : official publication of the Orthopaedic Research Society. 2022;. PM ID: 36250617
- See More