Exo-FlowMag96 96-well Magnetic Plate
Products
| Catalog Number | Description | Size | Price | Quantity | Add to Cart | |||
|---|---|---|---|---|---|---|---|---|
| EXOFLOWMAG-1 | Exo-FlowMag96 96-well magnetic plate | 1 Kit | $657 |
|
||||
Overview
Overview
Simplify your bead-based immunopurifications
Validated to work with any of our Exo-Flow32 or Exo-Flow96 IP Kits, the Exo-FlowMag96 Magnetic Plate simplifies plate-based exosome isolation using magnetic beads.
Use the Exo-Flow Multifunctional Magnetic Stand with any of our Exo-Flow Capture Kits:
| Cat.# | Kit |
|---|---|
| EXOFLOW100A-1 | CD9 Exo-Flow Capture Kit |
| EXOFLOW150A-1 | Tetraspanin Exo-Flow Combo Capture Kit |
| EXOFLOW200A-1 | CD31 Exo-Flow Capture Kit |
| EXOFLOW210A-1 | CD44 Exo-Flow Capture Kit |
| EXOFLOW300A-1 | CD63 Exo-Flow Capture Kit |
| EXOFLOW400A-1 | CD81 Exo-Flow Capture Kit |
| EXOFLOW500A-1 | Rab5b Exo-Flow Capture Kit |
| EXOFLOW600A-1 | HLA-G Exo-Flow Capture Kit |
| EXOFLOW610A-1 | CD14 Exo-Flow Capture Kit |
| EXOFLOW620A-1 | CD68 Exo-Flow Capture Kit |
| EXOFLOW630A-1 | EpCAM Exo-Flow Capture Kit |
| EXOFLOW660A-1 | CD73 Exo-Flow Capture Kit |
References
How It Works
How It Works
Take a closer look at the Exo-FlowMag96 96-well Magnetic Plate
Once the Exo-Flow32 or Exo-Flow96 clear plastic plate is placed into the Exo-FlowMag96 96-well Magnetic Plate, the magnetic beads stick to the bottom of each well simplifying removal of buffer for washing and elution.
Supporting Data
Supporting Data
See isolation data using Exo-FLOW IP Kits
For these studies, either human serum or HEK293 exosomes concentrated from cell culture media using ExoQuick-TC were added to the antibody-coupled magnetic beads. After washing, exosomes were eluted and recovery estimated using a standard BCA protein assay.
Figure 1. CD63 and CD9, two exosome markers, are readily detected in samples purified using Exo-Flow Kits. Approximately 1 µg of protein was loaded per well on a 4-20% gradient protein PAGE. The proteins were separated and transferred to nitrocellulose membranes for Western blot analysis. The blots were probed with either with anti-CD63 or anti-CD9 antibodies to detect the exosome protein markers.
Figure 2. NanoSight analysis shows Exo-Flow IP Kits deliver good yields of particles whose sizes are consistent with exosomes.
FAQs
Resources
Citations
Related Products
Products
| Catalog Number | Description | Size | Price | Quantity | Add to Cart | |||
|---|---|---|---|---|---|---|---|---|
| EXOFLOWMAG-1 | Exo-FlowMag96 96-well magnetic plate | 1 Kit | $657 |
|
||||
Overview
Overview
Simplify your bead-based immunopurifications
Validated to work with any of our Exo-Flow32 or Exo-Flow96 IP Kits, the Exo-FlowMag96 Magnetic Plate simplifies plate-based exosome isolation using magnetic beads.
Use the Exo-Flow Multifunctional Magnetic Stand with any of our Exo-Flow Capture Kits:
| Cat.# | Kit |
|---|---|
| EXOFLOW100A-1 | CD9 Exo-Flow Capture Kit |
| EXOFLOW150A-1 | Tetraspanin Exo-Flow Combo Capture Kit |
| EXOFLOW200A-1 | CD31 Exo-Flow Capture Kit |
| EXOFLOW210A-1 | CD44 Exo-Flow Capture Kit |
| EXOFLOW300A-1 | CD63 Exo-Flow Capture Kit |
| EXOFLOW400A-1 | CD81 Exo-Flow Capture Kit |
| EXOFLOW500A-1 | Rab5b Exo-Flow Capture Kit |
| EXOFLOW600A-1 | HLA-G Exo-Flow Capture Kit |
| EXOFLOW610A-1 | CD14 Exo-Flow Capture Kit |
| EXOFLOW620A-1 | CD68 Exo-Flow Capture Kit |
| EXOFLOW630A-1 | EpCAM Exo-Flow Capture Kit |
| EXOFLOW660A-1 | CD73 Exo-Flow Capture Kit |
References
How It Works
How It Works
Take a closer look at the Exo-FlowMag96 96-well Magnetic Plate
Once the Exo-Flow32 or Exo-Flow96 clear plastic plate is placed into the Exo-FlowMag96 96-well Magnetic Plate, the magnetic beads stick to the bottom of each well simplifying removal of buffer for washing and elution.
Supporting Data
Supporting Data
See isolation data using Exo-FLOW IP Kits
For these studies, either human serum or HEK293 exosomes concentrated from cell culture media using ExoQuick-TC were added to the antibody-coupled magnetic beads. After washing, exosomes were eluted and recovery estimated using a standard BCA protein assay.
Figure 1. CD63 and CD9, two exosome markers, are readily detected in samples purified using Exo-Flow Kits. Approximately 1 µg of protein was loaded per well on a 4-20% gradient protein PAGE. The proteins were separated and transferred to nitrocellulose membranes for Western blot analysis. The blots were probed with either with anti-CD63 or anti-CD9 antibodies to detect the exosome protein markers.
Figure 2. NanoSight analysis shows Exo-Flow IP Kits deliver good yields of particles whose sizes are consistent with exosomes.