ExoGlow™-Membrane EV Labeling Kit

Label EV membranes (465 nm excitation/635 nm emission) with a reagent specifically optimized for EV labeling.
  • Specific—carefully developed to generate a robust signal specific for EV membranes, leading to very low levels of background
  • Compatible—delivers robust performance on EVs isolated using all methods tested—including ExoQuick®, ultracentrifugation, and column-based workflows
  • Easy-to-use—labeling protocol is quick and straightforward
  • Powerful—can be used with as little as 1 µg of EVs

Products

Catalog Number Description Size Price Quantity Add to Cart
EXOGM600A-1 ExoGlowTM-Membrane EV Labeling Kit 25 Reactions $340
- +

Overview

Overview

See EVs better with reagents specifically optimized for labeling SBI’s new generation of extracellular vesicle (EV) labeling reagents take your EV visualization to new levels of clarity, with low background and high selectivity. Unlike general-purpose labeling reagents that are not optimized for EVs and suffer from high levels of background signal, ExoGlow™-Membrane improves your ability to track and localize EVs by specifically labeling EV membranes with a proprietary fluorescent dye that delivers very low levels of background signal. The result is unmatched EV imaging for more accurate studies (see below for our full range of next generation ExoGlow reagents).
  • Specific—carefully developed to generate a robust signal specific for EV membranes, leading to very low levels of background
  • Compatible—delivers robust performance on EVs isolated using all methods tested—including ExoQuick®, ultracentrifugation, and column-based workflows
  • Easy-to-use—labeling protocol is quick and straightforward
  • Powerful—can be used with as little as 1 µg of EVs
ExoGlow-Membrane properties: Excitation: 465 nm
Emission: 635 nm
Laser line: 488 nm Available next generation ExoGlow EV imaging reagents
EV Component LabeledCat. #ProductExcitation (nm)Emission (nm)Laser Line (nm)
Internal proteinEXOGP100A-1ExoGlow-Protein EV Labeling Kit (Red)573
588561
EXOGP300A-1ExoGlow-Protein EV Labeling Kit (Green)

511525488
EXOGP400A-1ExoGlow-Protein EV Labeling Kit (Blue)403454
405
RNAEXOGR800A-1ExoGlow-RNA EV Labeling Kit485537488
MembraneEXOGM600A-1ExoGlow-Membrane EV Labeling Kit465635488

References

How It Works

Supporting Data

Supporting Data

Imaging EVs with a new level of clarity

ExoGlow-Membranes enables clear visualization of labeled EVs being internalized by target cells

Figure 1. ExoGlow-Membrane enables clear visualization of labeled EVs being internalized by target cells. We labeled HEK293T EVs with ExoGlow-Membrane and followed uptake by HEK293T cells. The evenly distributed fluorescence signal shows internalization of labeled EVs by the target cells and the distribution of EV membranes to cellular membranes.

ExoGlow-Membranes enables clear visualization of labeled EVs being internalized by target cells

Figure 2. ExoGlow-Membrane is specific for intact membranes. We incubated either PBS, liposomes, liposomes pre-solubilized with detergent (0.05% Tx100), EVs, or lipoproteins. Only the samples with intact membranes—liposomes and EVs (blue)—show a high fluorescence signal, demonstrating ExoGlow-Membrane’s specificity for intact membranes.

FAQs

Resources

Citations

Products

Catalog Number Description Size Price Quantity Add to Cart
EXOGM600A-1 ExoGlowTM-Membrane EV Labeling Kit 25 Reactions $340
- +

Overview

Overview

See EVs better with reagents specifically optimized for labeling SBI’s new generation of extracellular vesicle (EV) labeling reagents take your EV visualization to new levels of clarity, with low background and high selectivity. Unlike general-purpose labeling reagents that are not optimized for EVs and suffer from high levels of background signal, ExoGlow™-Membrane improves your ability to track and localize EVs by specifically labeling EV membranes with a proprietary fluorescent dye that delivers very low levels of background signal. The result is unmatched EV imaging for more accurate studies (see below for our full range of next generation ExoGlow reagents).
  • Specific—carefully developed to generate a robust signal specific for EV membranes, leading to very low levels of background
  • Compatible—delivers robust performance on EVs isolated using all methods tested—including ExoQuick®, ultracentrifugation, and column-based workflows
  • Easy-to-use—labeling protocol is quick and straightforward
  • Powerful—can be used with as little as 1 µg of EVs
ExoGlow-Membrane properties: Excitation: 465 nm
Emission: 635 nm
Laser line: 488 nm Available next generation ExoGlow EV imaging reagents
EV Component LabeledCat. #ProductExcitation (nm)Emission (nm)Laser Line (nm)
Internal proteinEXOGP100A-1ExoGlow-Protein EV Labeling Kit (Red)573
588561
EXOGP300A-1ExoGlow-Protein EV Labeling Kit (Green)

511525488
EXOGP400A-1ExoGlow-Protein EV Labeling Kit (Blue)403454
405
RNAEXOGR800A-1ExoGlow-RNA EV Labeling Kit485537488
MembraneEXOGM600A-1ExoGlow-Membrane EV Labeling Kit465635488

References

How It Works

Supporting Data

Supporting Data

Imaging EVs with a new level of clarity

ExoGlow-Membranes enables clear visualization of labeled EVs being internalized by target cells

Figure 1. ExoGlow-Membrane enables clear visualization of labeled EVs being internalized by target cells. We labeled HEK293T EVs with ExoGlow-Membrane and followed uptake by HEK293T cells. The evenly distributed fluorescence signal shows internalization of labeled EVs by the target cells and the distribution of EV membranes to cellular membranes.

ExoGlow-Membranes enables clear visualization of labeled EVs being internalized by target cells

Figure 2. ExoGlow-Membrane is specific for intact membranes. We incubated either PBS, liposomes, liposomes pre-solubilized with detergent (0.05% Tx100), EVs, or lipoproteins. Only the samples with intact membranes—liposomes and EVs (blue)—show a high fluorescence signal, demonstrating ExoGlow-Membrane’s specificity for intact membranes.

FAQs

Citations