ExoGlow™-RNA EV Labeling Kit

Label EV mRNA (485 nm excitation/537 nm emission) with a reagent specifically optimized for EV labeling.
  • Specific—carefully developed to generate a robust signal specific for EV mRNAs, leading to very low levels of background
  • Compatible—delivers robust performance on EVs isolated using all methods tested—including ExoQuick®, ultracentrifugation, and column-based workflows
  • Easy-to-use—labeling protocol is quick and straightforward
  • Powerful—can be used with as little as 50 µg of EVs

Products

Catalog Number Description Size Price Quantity Add to Cart
EXOGR800A-1 ExoGlowTM-RNA EV Labeling Kit 10 Reactions $571
- +

Overview

Overview

See EVs better with reagents specifically optimized for labeling SBI’s new generation of extracellular vesicle (EV) labeling reagents take your EV visualization to new levels of clarity, with low background and high selectivity. Unlike general-purpose labeling reagents that are not optimized for EVs and suffer from high levels of background signal, ExoGlow™-RNA improves your ability to track and localize EVs by specifically labeling EV mRNAs with a proprietary fluorescent dye that delivers very low levels of background signal. The result is unmatched EV imaging for more accurate EV studies (see below for our full range of next generation ExoGlow reagents).
  • Specific—carefully developed to generate a robust signal specific for EV mRNAs, leading to very low levels of background
  • Compatible—delivers robust performance on EVs isolated using all methods tested—including ExoQuick®, ultracentrifugation, and column-based workflows
  • Easy-to-use—labeling protocol is quick and straightforward
  • Powerful—can be used with as little as 50 µg of EVs
ExoGlow-RNA properties: Excitation: 485 nm
Emission: 537 nm
Laser line: 488 nm Available next generation ExoGlow EV imaging reagents
EV Component LabeledCat. #ProductExcitation (nm)Emission (nm)Laser Line (nm)
Internal proteinEXOGP100A-1ExoGlow-Protein EV Labeling Kit (Red)573
588561
EXOGP300A-1ExoGlow-Protein EV Labeling Kit (Green)

511525488
EXOGP400A-1ExoGlow-Protein EV Labeling Kit (Blue)403454
405
RNAEXOGR800A-1ExoGlow-RNA EV Labeling Kit485537488
MembraneEXOGM600A-1ExoGlow-Membrane EV Labeling Kit465635488

References

How It Works

Supporting Data

Supporting Data

Imaging EVs with a new level of clarity

ExoGlow-RNA enables clear visualization of labeled EVs being internalized by target cells

Figure 1. ExoGlow-RNA enables clear visualization of labeled EVs being internalized by target cells. We labeled HEK293T EVs with ExoGlow-RNA and followed uptake by HEK293T cells. The fluorescence signal localizes to perinuclear and cytoplasmic regions, as would be expected for mRNA.

ExoGlow-RNA specifically labels mRNA

Figure 2. ExoGlow-RNA specifically labels mRNA. We used ExoGlow-RNA to label total RNA isolated from HEK293T cells, and then specifically depleted mRNA using polyT magnetic beads. The almost complete loss of signal in the mRNA-depleted sample demonstrates the specificity of ExoGlow-RNA dye for mRNA.

FAQs

Resources

Citations

Products

Catalog Number Description Size Price Quantity Add to Cart
EXOGR800A-1 ExoGlowTM-RNA EV Labeling Kit 10 Reactions $571
- +

Overview

Overview

See EVs better with reagents specifically optimized for labeling SBI’s new generation of extracellular vesicle (EV) labeling reagents take your EV visualization to new levels of clarity, with low background and high selectivity. Unlike general-purpose labeling reagents that are not optimized for EVs and suffer from high levels of background signal, ExoGlow™-RNA improves your ability to track and localize EVs by specifically labeling EV mRNAs with a proprietary fluorescent dye that delivers very low levels of background signal. The result is unmatched EV imaging for more accurate EV studies (see below for our full range of next generation ExoGlow reagents).
  • Specific—carefully developed to generate a robust signal specific for EV mRNAs, leading to very low levels of background
  • Compatible—delivers robust performance on EVs isolated using all methods tested—including ExoQuick®, ultracentrifugation, and column-based workflows
  • Easy-to-use—labeling protocol is quick and straightforward
  • Powerful—can be used with as little as 50 µg of EVs
ExoGlow-RNA properties: Excitation: 485 nm
Emission: 537 nm
Laser line: 488 nm Available next generation ExoGlow EV imaging reagents
EV Component LabeledCat. #ProductExcitation (nm)Emission (nm)Laser Line (nm)
Internal proteinEXOGP100A-1ExoGlow-Protein EV Labeling Kit (Red)573
588561
EXOGP300A-1ExoGlow-Protein EV Labeling Kit (Green)

511525488
EXOGP400A-1ExoGlow-Protein EV Labeling Kit (Blue)403454
405
RNAEXOGR800A-1ExoGlow-RNA EV Labeling Kit485537488
MembraneEXOGM600A-1ExoGlow-Membrane EV Labeling Kit465635488

References

How It Works

Supporting Data

Supporting Data

Imaging EVs with a new level of clarity

ExoGlow-RNA enables clear visualization of labeled EVs being internalized by target cells

Figure 1. ExoGlow-RNA enables clear visualization of labeled EVs being internalized by target cells. We labeled HEK293T EVs with ExoGlow-RNA and followed uptake by HEK293T cells. The fluorescence signal localizes to perinuclear and cytoplasmic regions, as would be expected for mRNA.

ExoGlow-RNA specifically labels mRNA

Figure 2. ExoGlow-RNA specifically labels mRNA. We used ExoGlow-RNA to label total RNA isolated from HEK293T cells, and then specifically depleted mRNA using polyT magnetic beads. The almost complete loss of signal in the mRNA-depleted sample demonstrates the specificity of ExoGlow-RNA dye for mRNA.

FAQs

Citations