Exosome Cyto-Tracer, pCT-CD9-GFP

Label exosomes with GFP by expressing the pCT-CD9-GFP Cyto-Tracer in the parent cells. Great for tracking exosomes and monitoring cargo delivery.
  • Stable lentivector-based system
  • Ideal for co-localization studies
  • Monitor exosome dynamics and functional studies in real time
  • Label exosomes from primary cells, tumor cells, stem cells, and more

Products

Catalog Number Description Size Price Quantity Add to Cart
CYTO122-PA-1 pCT-CD9-GFP (pCMV, Exosome/Secretory, CD9 Tetraspanin Tag) 10 µg $643
- +
CYTO122-VA-1 pCT-CD9-GFP (pCMV, Exosome/Secretory, CD9 Tetraspanin Tag; pre-packaged virus) >2x10^6 IFUs $741
- +

Overview

Overview

Easily track exosomes with a CD9-GFP fusion

Monitor exosome dynamics with the pCT-CD9-GFP (pCMV) Exosome Cyto-Tracer (also available as a CD9-RFP fusion, pCT-CD9-RFP). Provided as either lentivector or pre-packaged virus, this Cyto-Tracer fuses GFP to the tetraspanin CD9, which then gets packaged into exosomes produced by the transfected cells. Great for creating stable cell lines that produce GFP-labeled exosomes as well as labeling exosomes from primary cells, tumor cells, stem cells, and more.

  • Stable lentivector-based system
  • Ideal for co-localization studies
  • Monitor exosome dynamics and functional studies in real time
  • Label exosomes from primary cells, tumor cells, stem cells, and more
Choose the exosome quantitation method that’s best for your studies
Promoter (Cat.#)Cyto-TracerTarget LocationPeptide Tag
CMV (CYTO100-PA-1/CYTO100-VA-1)
MSCV (CYTO100-PB-1/CYTO100-VB-1)
pCT-Mem-GFPPlasma MembraneNeuromodulin
CMV (CYTO101-PA-1/CYTO101-VA-1)
MSCV (CYTO101-PB-1/CYTO101-VB-1)
pCT-InnerMem-GFPInner Leaflet of MembraneFarnesylation signal
CMV (CYTO102-PA-1/CYTO102-VA-1)
MSCV (CYTO102-PB-1/CYTO102-VB-1)
pCT-Mito-GFPMitochondriaCytochrome C oxidase VIII
CMV (CYTO103-PA-1/CYTO103-VA-1)pCT-ER-GFPEndoplasmic ReticulumER targeting consensus
CMV (CYTO104-PA-1/CYTO104-VA-1)pCT-Golgi-GFPGolgi ApparatusGalactosyltrasferase
CMV (CYTO105-PA-1/CYTO105-VA-1)pCT-Secretory-GFPER-Golgi  VesiclesSecretory consensus peptide
CMV (CYTO106-PA-1/CYTO106-VA-1)pCT-Lyso-GFPLysosomeCathepsin B
CMV (CYTO107-PA-1/CYTO107-VA-1)pCT-Endo-GFPEndosomeRhoB
CMV (CYTO107R-PA-1/CYTO107R-VA-1)pCT-Endo-RFPEndosomeRhoB
CMV (CYTO108-PA-1/CYTO108-VA-1)
MSCV (CYTO108-PB-1/CYTO108-VB-1)
pCT-Pero-GFPPeroxisomePeroxisomal consensus signal
CMV (CYTO109-PA-1/CYTO109-VA-1)pCT-Autophago-GFP
AutophagosomeLC3
CMV (CYTO109R-PA-1/CYTO109R-VA-1)pCT-Autophago-RFPAutophagosomeLC3
CMV (CYTO120-PA-1/CYTO120-VA-1)pCT-CD63-GFPExosome/SecretoryCD63 Tetraspanin
CMV (CYTO120R-PA-1/CYTO120R-VA-1)pCT-CD63-RFPExosome/SecretoryCD63 Tetraspanin
CMV (CYTO122-PA-1/CYTO122-VA-1)pCT-CD9-GFPExosome/SecretoryCD9 Tetraspanin
CMV (CYTO123-PA-1/CYTO123-VA-1)pCT-CD9-RFPExosome/SecretoryCD9 Tetraspanin
CMV (CYTO124-PA-1/CYTO124-VA-1)pCT-CD81-GFPExosome/SecretoryCD81 Tetraspanin
CMV (CYTO125-PA-1/CYTO125-VA-1)pCT-CD81-RFPExosome/SecretoryCD81 Tetraspanin
CMV (CYTO110-PA-1/CYTO110-VA-1)
MSCV (CYTO110-PB-1/CYTO110-VB-1)
pCT-Actin-GFPCytoskeletonβ-actin
CMV (CYTO111-PA-1/CYTO111-VA-1)
MSCV (CYTO111-PB-1/CYTO111-VB-1)
pCT-Tublin-GFPCytoskeletonα-tublin
CMV (CYTO111R-PA-1/CYTO111R-VA-1)pCT-Tublin-RFPCytoskeletonα-tublin
CMV (CYTO112-PA-1/CYTO112-VA-1)pCT-MAP4-GFPMicrotubuleMicrotubule associated protein 4 (MAP4)
CMV (CYTO113-PA-1/CYTO113-VA-1)
MSCV (CYTO113-PB-1/CYTO113-VB-1)
pCT-H2B-GFPNucleusH2B
CMV (CYTO114-PA-1/CYTO114-VA-1)pCT-Apoptosis-Luc Caspase 3/7 activationCyclic inactive luciferase
CMV (CYTO115-PA-1/CYTO115-VA-1)pCT-GFP-BAXCytosol to MitochondriaBAX
CMV (CYTO116-PA-1/CYTO116-VA-1)pCT- Catenin-GFPCytosol to Nucleus/Cell Membraneβ-Catenin activation
CMV (CYTO117-PA-1/CYTO117-VA-1)pCT-CMV-PSD95-GFPDendrite MembranesPSD-95
CMV (CYTO118-PA-1/CYTO118-VA-1)
MSCV (CYTO118-PB-1/CYTO118-VB-1)
pCT-Cyto-GFPCytosolic CompartmentGFP-Untagged
CMV (CYTO119-PA-1/CYTO119-VA-1)pCT-Cyto-RFPCytosolic CompartmentRFP-Untagged
MSCV (CYTO121-PB-1/CYTO121-VB-1)pCT-Cyto-GFpLucGFP and Luciferase ControlGFP and Luciferase (Untagged)

How It Works

Supporting Data

Supporting Data

See some of our exosome Cyto-Tracers in action

The following figure and videos are from:

Garcia NA, et al. Glucose Starvation in Cardiomyocytes Enhances Exosome Secretion and Promotes Angiogenesis in Endothelial Cells. PLoS ONE. 2015. 10(9). PMCID: PMC4578916.

See exosomes being transferred from cardiomyocytes to mouse endothelial cells and human cord blood endothelial cells (HUVEC)

Figure 4 from Garcia, et al. Exosome transfer from CMs to ECs. (A) H9c2 transfected with pCT-CD63-GFP. FACS analysis of 90% GFP positive cells. (B) Representative images from confocal time-lapse microscopy of mouse ECs (ACTB-DsRed) (EC; red) co-cultured with H9C2-CD63-GFP cells (green), previously cultured for 24 h in +/-St medium. Exosome transfer from H9C2 CMs to EC can be observed (S1 and S2 Movies, below). White arrows show CD63-GFP structures inside ECs (C) Representative immunostaining of H9C2-CD63-GFP and HUVEC co-cultures; anti-GFP (green) and anti-CD31 (red). The images illustrate GFP fluorescence from CD63-GFP exosomes in CD31-positive cells (red) after 24 h incubation in +/-St medium. White arrows show CD63-GFP structures inside ECs.


Video S1 from Garcia, et al. Exosome transfer from H9C2-CD63-GFP (green) to endothelial DsRed cells (red) under +St conditions.

Video S2 from Garcia, et al. Exosome transfer from H9C2-CD63-GFP (green) to endothelial DsRed cells (red) under -St conditions.

Exosomes labeled with CD63-GFP (top panels) and co-transfected with CD9-GFP and CD9-RFP (bottom panels)

Labeled exosomes. (Top panels) CD63-GFP Cyto-Tracers transfected into a human fibrosarcoma cell line. (Bottom panels) CD9-GFP and CD9-RFP Cyto-Tracers co-transfected into HEK293 cells.

FAQs

Resources

Citations

  • Kessler, M, et al. (2019) Chronic Chlamydia infection in human organoids increases stemness and promotes age-dependent CpG methylation. Nat Commun. 2019; 10(1):1194. PM ID: 30886143
  • Gonzalez-King, H, et al. (2017) Hypoxia Inducible Factor-1α Potentiates Jagged 1-Mediated Angiogenesis by Mesenchymal Stem Cell-Derived Exosomes. Stem Cells. 2017; 35(7):1747-1759. PM ID: 28376567
  • Jawhari, S, et al. (2017) Autophagy and TrkC/NT-3 signaling joined forces boost the hypoxic glioblastoma cell survival. Carcinogenesis. 2017; 38(6):592-603. PM ID: 28402394
  • Soares, VYR. (2017) O papel das vesículas extracelulares na fisiopatologia da perda auditiva ocasionada pelo Schwannoma vestibular. Thesis. 2017;. Link: Thesis
  • Braganza, A, et al. (2017) UBE3B Is a Calmodulin-regulated, Mitochondrion-associated E3 Ubiquitin Ligase. J. Biol. Chem.. 2017; 292(6):2470-2484. PM ID: 28003368
  • Yoon, C, et al. (2016) Delivery of miR-155 to retinal pigment epithelial cells mediated by Burkitt’s lymphoma exosomes. Tumour Biol.. 2016; 37(1):313-21. PM ID: 26211004
  • Lo Cicero, A, et al. (2015) Exosomes released by keratinocytes modulate melanocyte pigmentation. Nat Commun. 2015; 6:7506. PM ID: 26103923
  • Liang, Y. (2015) Exosome/Microvesicle Cargo Contents Recruitment in a Glycosylation-dependent Pathway. Thesis. 2015;. Link: Thesis
  • Jawhari, S. (2015) Etude des signalisations autophagique et neurotrophique dans des lignées de glioblastome humain activées lors de l’hypoxie. Thesis. 2015;. Link: Thesis
  • VARLET, P, PU-PH, P & DJAVAHERI-MERGNY, M. (2015) SOHA JAWHARI. Thesis. 2015;. Link: Thesis
  • Liang, Y, et al. (2014) Complex N-linked glycans serve as a determinant for exosome/microvesicle cargo recruitment. J. Biol. Chem.. 2014; 289(47):32526-37. PM ID: 25261472
  • Fang, Q, et al. (2014) HSP90 regulates DNA repair via the interaction between XRCC1 and DNA polymerase β. Nat Commun. 2014; 5:5513. PM ID: 25423885
  • Sato, Y, et al. (2014) MTCL1 crosslinks and stabilizes non-centrosomal microtubules on the Golgi membrane. Nat Commun. 2014; 5:5266. PM ID: 25366663

Products

Catalog Number Description Size Price Quantity Add to Cart
CYTO122-PA-1 pCT-CD9-GFP (pCMV, Exosome/Secretory, CD9 Tetraspanin Tag) 10 µg $643
- +
CYTO122-VA-1 pCT-CD9-GFP (pCMV, Exosome/Secretory, CD9 Tetraspanin Tag; pre-packaged virus) >2x10^6 IFUs $741
- +

Overview

Overview

Easily track exosomes with a CD9-GFP fusion

Monitor exosome dynamics with the pCT-CD9-GFP (pCMV) Exosome Cyto-Tracer (also available as a CD9-RFP fusion, pCT-CD9-RFP). Provided as either lentivector or pre-packaged virus, this Cyto-Tracer fuses GFP to the tetraspanin CD9, which then gets packaged into exosomes produced by the transfected cells. Great for creating stable cell lines that produce GFP-labeled exosomes as well as labeling exosomes from primary cells, tumor cells, stem cells, and more.

  • Stable lentivector-based system
  • Ideal for co-localization studies
  • Monitor exosome dynamics and functional studies in real time
  • Label exosomes from primary cells, tumor cells, stem cells, and more
Choose the exosome quantitation method that’s best for your studies
Promoter (Cat.#)Cyto-TracerTarget LocationPeptide Tag
CMV (CYTO100-PA-1/CYTO100-VA-1)
MSCV (CYTO100-PB-1/CYTO100-VB-1)
pCT-Mem-GFPPlasma MembraneNeuromodulin
CMV (CYTO101-PA-1/CYTO101-VA-1)
MSCV (CYTO101-PB-1/CYTO101-VB-1)
pCT-InnerMem-GFPInner Leaflet of MembraneFarnesylation signal
CMV (CYTO102-PA-1/CYTO102-VA-1)
MSCV (CYTO102-PB-1/CYTO102-VB-1)
pCT-Mito-GFPMitochondriaCytochrome C oxidase VIII
CMV (CYTO103-PA-1/CYTO103-VA-1)pCT-ER-GFPEndoplasmic ReticulumER targeting consensus
CMV (CYTO104-PA-1/CYTO104-VA-1)pCT-Golgi-GFPGolgi ApparatusGalactosyltrasferase
CMV (CYTO105-PA-1/CYTO105-VA-1)pCT-Secretory-GFPER-Golgi  VesiclesSecretory consensus peptide
CMV (CYTO106-PA-1/CYTO106-VA-1)pCT-Lyso-GFPLysosomeCathepsin B
CMV (CYTO107-PA-1/CYTO107-VA-1)pCT-Endo-GFPEndosomeRhoB
CMV (CYTO107R-PA-1/CYTO107R-VA-1)pCT-Endo-RFPEndosomeRhoB
CMV (CYTO108-PA-1/CYTO108-VA-1)
MSCV (CYTO108-PB-1/CYTO108-VB-1)
pCT-Pero-GFPPeroxisomePeroxisomal consensus signal
CMV (CYTO109-PA-1/CYTO109-VA-1)pCT-Autophago-GFP
AutophagosomeLC3
CMV (CYTO109R-PA-1/CYTO109R-VA-1)pCT-Autophago-RFPAutophagosomeLC3
CMV (CYTO120-PA-1/CYTO120-VA-1)pCT-CD63-GFPExosome/SecretoryCD63 Tetraspanin
CMV (CYTO120R-PA-1/CYTO120R-VA-1)pCT-CD63-RFPExosome/SecretoryCD63 Tetraspanin
CMV (CYTO122-PA-1/CYTO122-VA-1)pCT-CD9-GFPExosome/SecretoryCD9 Tetraspanin
CMV (CYTO123-PA-1/CYTO123-VA-1)pCT-CD9-RFPExosome/SecretoryCD9 Tetraspanin
CMV (CYTO124-PA-1/CYTO124-VA-1)pCT-CD81-GFPExosome/SecretoryCD81 Tetraspanin
CMV (CYTO125-PA-1/CYTO125-VA-1)pCT-CD81-RFPExosome/SecretoryCD81 Tetraspanin
CMV (CYTO110-PA-1/CYTO110-VA-1)
MSCV (CYTO110-PB-1/CYTO110-VB-1)
pCT-Actin-GFPCytoskeletonβ-actin
CMV (CYTO111-PA-1/CYTO111-VA-1)
MSCV (CYTO111-PB-1/CYTO111-VB-1)
pCT-Tublin-GFPCytoskeletonα-tublin
CMV (CYTO111R-PA-1/CYTO111R-VA-1)pCT-Tublin-RFPCytoskeletonα-tublin
CMV (CYTO112-PA-1/CYTO112-VA-1)pCT-MAP4-GFPMicrotubuleMicrotubule associated protein 4 (MAP4)
CMV (CYTO113-PA-1/CYTO113-VA-1)
MSCV (CYTO113-PB-1/CYTO113-VB-1)
pCT-H2B-GFPNucleusH2B
CMV (CYTO114-PA-1/CYTO114-VA-1)pCT-Apoptosis-Luc Caspase 3/7 activationCyclic inactive luciferase
CMV (CYTO115-PA-1/CYTO115-VA-1)pCT-GFP-BAXCytosol to MitochondriaBAX
CMV (CYTO116-PA-1/CYTO116-VA-1)pCT- Catenin-GFPCytosol to Nucleus/Cell Membraneβ-Catenin activation
CMV (CYTO117-PA-1/CYTO117-VA-1)pCT-CMV-PSD95-GFPDendrite MembranesPSD-95
CMV (CYTO118-PA-1/CYTO118-VA-1)
MSCV (CYTO118-PB-1/CYTO118-VB-1)
pCT-Cyto-GFPCytosolic CompartmentGFP-Untagged
CMV (CYTO119-PA-1/CYTO119-VA-1)pCT-Cyto-RFPCytosolic CompartmentRFP-Untagged
MSCV (CYTO121-PB-1/CYTO121-VB-1)pCT-Cyto-GFpLucGFP and Luciferase ControlGFP and Luciferase (Untagged)

How It Works

Supporting Data

Supporting Data

See some of our exosome Cyto-Tracers in action

The following figure and videos are from:

Garcia NA, et al. Glucose Starvation in Cardiomyocytes Enhances Exosome Secretion and Promotes Angiogenesis in Endothelial Cells. PLoS ONE. 2015. 10(9). PMCID: PMC4578916.

See exosomes being transferred from cardiomyocytes to mouse endothelial cells and human cord blood endothelial cells (HUVEC)

Figure 4 from Garcia, et al. Exosome transfer from CMs to ECs. (A) H9c2 transfected with pCT-CD63-GFP. FACS analysis of 90% GFP positive cells. (B) Representative images from confocal time-lapse microscopy of mouse ECs (ACTB-DsRed) (EC; red) co-cultured with H9C2-CD63-GFP cells (green), previously cultured for 24 h in +/-St medium. Exosome transfer from H9C2 CMs to EC can be observed (S1 and S2 Movies, below). White arrows show CD63-GFP structures inside ECs (C) Representative immunostaining of H9C2-CD63-GFP and HUVEC co-cultures; anti-GFP (green) and anti-CD31 (red). The images illustrate GFP fluorescence from CD63-GFP exosomes in CD31-positive cells (red) after 24 h incubation in +/-St medium. White arrows show CD63-GFP structures inside ECs.


Video S1 from Garcia, et al. Exosome transfer from H9C2-CD63-GFP (green) to endothelial DsRed cells (red) under +St conditions.

Video S2 from Garcia, et al. Exosome transfer from H9C2-CD63-GFP (green) to endothelial DsRed cells (red) under -St conditions.

Exosomes labeled with CD63-GFP (top panels) and co-transfected with CD9-GFP and CD9-RFP (bottom panels)

Labeled exosomes. (Top panels) CD63-GFP Cyto-Tracers transfected into a human fibrosarcoma cell line. (Bottom panels) CD9-GFP and CD9-RFP Cyto-Tracers co-transfected into HEK293 cells.

FAQs

Citations

  • Kessler, M, et al. (2019) Chronic Chlamydia infection in human organoids increases stemness and promotes age-dependent CpG methylation. Nat Commun. 2019; 10(1):1194. PM ID: 30886143
  • Gonzalez-King, H, et al. (2017) Hypoxia Inducible Factor-1α Potentiates Jagged 1-Mediated Angiogenesis by Mesenchymal Stem Cell-Derived Exosomes. Stem Cells. 2017; 35(7):1747-1759. PM ID: 28376567
  • Jawhari, S, et al. (2017) Autophagy and TrkC/NT-3 signaling joined forces boost the hypoxic glioblastoma cell survival. Carcinogenesis. 2017; 38(6):592-603. PM ID: 28402394
  • Soares, VYR. (2017) O papel das vesículas extracelulares na fisiopatologia da perda auditiva ocasionada pelo Schwannoma vestibular. Thesis. 2017;. Link: Thesis
  • Braganza, A, et al. (2017) UBE3B Is a Calmodulin-regulated, Mitochondrion-associated E3 Ubiquitin Ligase. J. Biol. Chem.. 2017; 292(6):2470-2484. PM ID: 28003368
  • Yoon, C, et al. (2016) Delivery of miR-155 to retinal pigment epithelial cells mediated by Burkitt’s lymphoma exosomes. Tumour Biol.. 2016; 37(1):313-21. PM ID: 26211004
  • Lo Cicero, A, et al. (2015) Exosomes released by keratinocytes modulate melanocyte pigmentation. Nat Commun. 2015; 6:7506. PM ID: 26103923
  • Liang, Y. (2015) Exosome/Microvesicle Cargo Contents Recruitment in a Glycosylation-dependent Pathway. Thesis. 2015;. Link: Thesis
  • Jawhari, S. (2015) Etude des signalisations autophagique et neurotrophique dans des lignées de glioblastome humain activées lors de l’hypoxie. Thesis. 2015;. Link: Thesis
  • VARLET, P, PU-PH, P & DJAVAHERI-MERGNY, M. (2015) SOHA JAWHARI. Thesis. 2015;. Link: Thesis
  • Liang, Y, et al. (2014) Complex N-linked glycans serve as a determinant for exosome/microvesicle cargo recruitment. J. Biol. Chem.. 2014; 289(47):32526-37. PM ID: 25261472
  • Fang, Q, et al. (2014) HSP90 regulates DNA repair via the interaction between XRCC1 and DNA polymerase β. Nat Commun. 2014; 5:5513. PM ID: 25423885
  • Sato, Y, et al. (2014) MTCL1 crosslinks and stabilizes non-centrosomal microtubules on the Golgi membrane. Nat Commun. 2014; 5:5266. PM ID: 25366663