ExoFlow-ONE EV Labeling Kit for Flow Cytometry (Sapphire Blue)
- Directly detect EVs by specifically labeling internal EV components
- Achieve near single-vesicle visualization* with our proprietary ExoFlow-ONE dyes that
deliver
- High quantum efficiency
- Negligible background signal when not bound to EVs
- Accurately estimate the size of labeled EVs and ensure proper run-to-run calibration with the included size standards
Products
Catalog Number | Description | Size | Price | Quantity | Add to Cart | |||
---|---|---|---|---|---|---|---|---|
EXOF400A-1 | ExoFlow-ONE EV Labeling Kit for Flow Cytometry, Sapphire Blue | 25 Reactions | $433 |
|
Overview
Overview
Harness the power of flow cytometry for EV analysis
Finally, you can take full advantage of the power of flow-based methods for analyzing extracellular vesicles (EVs) with SBI’s exclusive ExoFlow-ONE Gemstone dyes. By specifically labeling internal EV components with one of these proprietary, high quantum efficiency dyes, you can achieve near single-vesicle resolution* for more powerful flow cytometry and FACS studies, and uncover greater insights into EV biology.
ExoFlow-ONE EV Labeling Kits:
- Directly detect EVs by specifically labeling internal EV components
- Achieve near single-vesicle visualization* with our proprietary ExoFlow-ONE dyes that deliver
- High quantum efficiency
- Negligible background signal when not bound to EVs
- Accurately estimate the size of labeled EVs and ensure proper run-to-run calibration with the included size standards
- Learn more from a single sample by multiplexing with our range of spectrally separated ExoFlow-ONE dyes
The ExoFlow-ONE Sapphire Blue Gemstone Dye Kit comes with enough labeling reagents for 25 reactions**. Each kit also includes 1.5 mL of silica bead size standards (110 nm – 1300 nm), two of which are fluorescent. The silica beads, sourced from Apogee Flow Systems, are specifically designed to produce a refractive index and light scattering patterns similar to biological particles, enabling more accurate estimation of EV particle size.
*Requires a specialized flow cytometry imager capable of high-resolution vesicle analysis (e.g. Amnis® ImageStreamX Mark II, Apogee Micro-PLUS).
**Each reaction is defined as 1 µL of working dye combined with 200 – 500 µg of EV protein resuspended in 500 µL of buffer.
Choose the right ExoFlow-ONE dye for your project
Cat.# | ExoFlow ONE Dye | Excitation/Emission Wavelength | Recommended Laser Line |
---|---|---|---|
EXOF100A-1 | Ruby Red | 573/588 nm | 561 nm |
EXOF200A-1 | Garnet Far Red | 628/643 nm | 633 nm |
EXOF300A-1 | Emerald Green | 511/525 nm | 488 nm |
EXOF400A-1 | Sapphire Blue | 403/454 nm | 405 nm |
EXOF500A-1 | Citrine Yellow | 542/556 nm | 532 nm |
References
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Products
Catalog Number | Description | Size | Price | Quantity | Add to Cart | |||
---|---|---|---|---|---|---|---|---|
EXOF400A-1 | ExoFlow-ONE EV Labeling Kit for Flow Cytometry, Sapphire Blue | 25 Reactions | $433 |
|
Overview
Overview
Harness the power of flow cytometry for EV analysis
Finally, you can take full advantage of the power of flow-based methods for analyzing extracellular vesicles (EVs) with SBI’s exclusive ExoFlow-ONE Gemstone dyes. By specifically labeling internal EV components with one of these proprietary, high quantum efficiency dyes, you can achieve near single-vesicle resolution* for more powerful flow cytometry and FACS studies, and uncover greater insights into EV biology.
ExoFlow-ONE EV Labeling Kits:
- Directly detect EVs by specifically labeling internal EV components
- Achieve near single-vesicle visualization* with our proprietary ExoFlow-ONE dyes that deliver
- High quantum efficiency
- Negligible background signal when not bound to EVs
- Accurately estimate the size of labeled EVs and ensure proper run-to-run calibration with the included size standards
- Learn more from a single sample by multiplexing with our range of spectrally separated ExoFlow-ONE dyes
The ExoFlow-ONE Sapphire Blue Gemstone Dye Kit comes with enough labeling reagents for 25 reactions**. Each kit also includes 1.5 mL of silica bead size standards (110 nm – 1300 nm), two of which are fluorescent. The silica beads, sourced from Apogee Flow Systems, are specifically designed to produce a refractive index and light scattering patterns similar to biological particles, enabling more accurate estimation of EV particle size.
*Requires a specialized flow cytometry imager capable of high-resolution vesicle analysis (e.g. Amnis® ImageStreamX Mark II, Apogee Micro-PLUS).
**Each reaction is defined as 1 µL of working dye combined with 200 – 500 µg of EV protein resuspended in 500 µL of buffer.
Choose the right ExoFlow-ONE dye for your project
Cat.# | ExoFlow ONE Dye | Excitation/Emission Wavelength | Recommended Laser Line |
---|---|---|---|
EXOF100A-1 | Ruby Red | 573/588 nm | 561 nm |
EXOF200A-1 | Garnet Far Red | 628/643 nm | 633 nm |
EXOF300A-1 | Emerald Green | 511/525 nm | 488 nm |
EXOF400A-1 | Sapphire Blue | 403/454 nm | 405 nm |
EXOF500A-1 | Citrine Yellow | 542/556 nm | 532 nm |