XPack MSCV-XP-MCS-EF1α-Puro Cloning Lentivector
- Powerful—enables a wealth of applications, including
- Exosome labeling
- Exosome cargo tracking
- Exosomes as cargo delivery vehicles
- Exosomes as biotherapeutics
- Exosome-generating stable cell lines with protein cargo-of-interest
- Optimized—SBI developed the XPack peptide tag for robust exosome targeting
- Reliable—based on SBI’s highly-cited, well-proven lentivector technology
- Versatile
- Two promoter options for expression in a wide range of cells
- Comes as pre-built reporters (GFP, RFP, luciferase) or ready-to-customize cloning lentivectors
Products
Catalog Number | Description | Size | Price | Quantity | Add to Cart | |||
---|---|---|---|---|---|---|---|---|
XPAK710PA-1 | XPack MSCV-XP-MCS-EF1α-Puro Cloning Lentivector | 10 µg | $757 |
|
Overview
Overview
Turn your protein into exosome cargo with XPack™
Whether you’re engineering exosomes as protein delivery vehicles, labeling exosomes with a custom reporter protein, or performing functional studies, XPack gives you the power to package your protein-of-interest into exosomes.
With the XPack MSCV-XP-MCS-EF1α-Puro Cloning Lentivector, you can use SBI’s well-proven lentivector technology to express your protein-of-interest and package it into exosomes. The strong MSCV promoter delivers high expression levels in hematopoietic and stem cell lines and the multiple cloning site downstream of the XPack tag enables fusion of the exosome-targeting peptide to your protein-of-interest (also available with a CMV promoter for expression in hematopoietic and stem cells).
The XPack System at a glance:
- Powerful—enables a wealth of applications, including
- Exosome labeling
- Exosome cargo tracking
- Exosomes as cargo delivery vehicles
- Exosomes as biotherapeutics
- Exosome-generating stable cell lines with protein cargo-of-interest
- Optimized—SBI developed the XPack peptide tag for robust exosome targeting
- Reliable—based on SBI’s highly-cited, well-proven lentivector technology
- Versatile
- Two promoter options for expression in a wide range of cells
- Comes as pre-built reporters (GFP, RFP, luciferase) or ready-to-customize cloning lentivectors
Reporter | CMV Promoter (For most cell lines) | MSCV Promoter (For hematopoietic and stem cell lines) | ||
---|---|---|---|---|
Customize with our cloning vector |
||||
XPAK510PA-1 | XPack CMV-XP-MCS-EF1α-Puro | XPAK710PA-1 | XPack MSCV-XP-MCS-EF1α-Puro | |
Label exosomes with pre-built reporters |
||||
GFP | XPAK530CL-1 XPAK530PA-1 XPAK530VA-1 | XPack CMV-XP-GFP-EF1α-Puro | XPAK730PA-1 XPAK730VA-1 | XPack MSCV-XP-GFP-EF1α-Puro |
RFP | XPAK531PA-1 | XPack CMV-XP-RFP-EF1α-Puro | ||
Luciferase | XPAK532CL-1 XPAK532PA-1 XPAK532VA-1 | XPack CMV-XP-Luciferase-EF1α-Puro | XPAK732PA-1 XPAK732VA-1 | XPack MSCV-XP-Luciferase-EF1α-Puro |
References
How It Works
How It Works
Optimized for success
With XPack, SBI has developed an optimized N-terminal peptide sequence that targets the peptide—and any protein fused to it—to the inner surface of the exosome membrane. Clone your protein of interest into one of the XPack Cloning Vectors and, when expressed, your protein will be packaged into exosomes.
Supporting Data
Supporting Data
Engineer exosomes with XPack and deliver functional protein to cells, changing cellular physiology
Cyclin-dependent kinase inhibitor 1B (CDKN1B) is a 22 KDa protein which is a cell cycle inhibitor and tumor suppressor. To demonstrate how exosomes can be used to deliver functional protein to cells, we generated exosomes containing CDKN1B and show that addition of these exosomes HEK-293 cells can reduce cellular proliferation (Figure 1).
FAQs
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Related Products
Products
Catalog Number | Description | Size | Price | Quantity | Add to Cart | |||
---|---|---|---|---|---|---|---|---|
XPAK710PA-1 | XPack MSCV-XP-MCS-EF1α-Puro Cloning Lentivector | 10 µg | $757 |
|
Overview
Overview
Turn your protein into exosome cargo with XPack™
Whether you’re engineering exosomes as protein delivery vehicles, labeling exosomes with a custom reporter protein, or performing functional studies, XPack gives you the power to package your protein-of-interest into exosomes.
With the XPack MSCV-XP-MCS-EF1α-Puro Cloning Lentivector, you can use SBI’s well-proven lentivector technology to express your protein-of-interest and package it into exosomes. The strong MSCV promoter delivers high expression levels in hematopoietic and stem cell lines and the multiple cloning site downstream of the XPack tag enables fusion of the exosome-targeting peptide to your protein-of-interest (also available with a CMV promoter for expression in hematopoietic and stem cells).
The XPack System at a glance:
- Powerful—enables a wealth of applications, including
- Exosome labeling
- Exosome cargo tracking
- Exosomes as cargo delivery vehicles
- Exosomes as biotherapeutics
- Exosome-generating stable cell lines with protein cargo-of-interest
- Optimized—SBI developed the XPack peptide tag for robust exosome targeting
- Reliable—based on SBI’s highly-cited, well-proven lentivector technology
- Versatile
- Two promoter options for expression in a wide range of cells
- Comes as pre-built reporters (GFP, RFP, luciferase) or ready-to-customize cloning lentivectors
Reporter | CMV Promoter (For most cell lines) | MSCV Promoter (For hematopoietic and stem cell lines) | ||
---|---|---|---|---|
Customize with our cloning vector |
||||
XPAK510PA-1 | XPack CMV-XP-MCS-EF1α-Puro | XPAK710PA-1 | XPack MSCV-XP-MCS-EF1α-Puro | |
Label exosomes with pre-built reporters |
||||
GFP | XPAK530CL-1 XPAK530PA-1 XPAK530VA-1 | XPack CMV-XP-GFP-EF1α-Puro | XPAK730PA-1 XPAK730VA-1 | XPack MSCV-XP-GFP-EF1α-Puro |
RFP | XPAK531PA-1 | XPack CMV-XP-RFP-EF1α-Puro | ||
Luciferase | XPAK532CL-1 XPAK532PA-1 XPAK532VA-1 | XPack CMV-XP-Luciferase-EF1α-Puro | XPAK732PA-1 XPAK732VA-1 | XPack MSCV-XP-Luciferase-EF1α-Puro |
References
How It Works
How It Works
Optimized for success
With XPack, SBI has developed an optimized N-terminal peptide sequence that targets the peptide—and any protein fused to it—to the inner surface of the exosome membrane. Clone your protein of interest into one of the XPack Cloning Vectors and, when expressed, your protein will be packaged into exosomes.
Supporting Data
Supporting Data
Engineer exosomes with XPack and deliver functional protein to cells, changing cellular physiology
Cyclin-dependent kinase inhibitor 1B (CDKN1B) is a 22 KDa protein which is a cell cycle inhibitor and tumor suppressor. To demonstrate how exosomes can be used to deliver functional protein to cells, we generated exosomes containing CDKN1B and show that addition of these exosomes HEK-293 cells can reduce cellular proliferation (Figure 1).