ExoELISA Complete Kit (CD63 Detection)
- Sensitive—detect as little as 500 µg protein equivalent
- Flexible—compatible with all major exosome isolation methods (g. ExoQuick®, ultracentrifugation, ultrafiltration, and immunoaffinity capture) from human, mouse, and rat
- Quantitative—calibrated internal standards enable quantitation of exosomes carrying CD63
Products
Overview
Overview
Sensitive, ELISA-based exosome quantitation
When you would like to use an ELISA-based method to find out how many exosomes you have and time is not limiting, choose one of SBI’s ExoELISA Complete Kits. Calibrated using NanoSight Analysis, the ExoELISA Complete Kit (CD63 Detection) enables calculation of exosome abundance based on the presence of CD63, a general exosome marker. Note that our newer ExoELISA-ULTRA Complete Kit (CD63 Detection) can provide faster exosome quantitation using significantly less sample.
- Sensitive—detect as little as 500 µg protein equivalent
- Flexible—compatible with all major exosome isolation methods (g. ExoQuick®, ultracentrifugation, ultrafiltration, and immunoaffinity capture) from human, mouse, and rat
- Quantitative—calibrated internal standards enable quantitation of exosomes carrying CD63
References
How It Works
How It Works
Our ExoELISA Kits have all the reagents you need to run the ELISA—just add lysed exosome particles. The kits are compatible with exosomes isolated using most methods, including ExoQuick®, ExoQuick-TC®, or ultracentrifugation.
The lysed exosome particles (and, thus, exosomal proteins) are directly immobilized onto the wells of the microtiter plate, and after binding, a blocking agent is added to prevent non-specific binding of the primary detection antibody, anti-CD63. Following addition of anti-CD63, a secondary antibody (goat anti-rabbit) linked to horseradish peroxidase (HRP) is also added to amplify the signal and increase assay sensitivity.
The amount of CD63 is measured via activity of the bound HRP-secondary antibody using a colorimetric assay with extra-sensitive TMB as the substrate. The accumulation of colored product is proportional to the amount of CD63 present in each well, and is measured using a microtiter plate reader at 450 nm absorbance.
Each ExoELISA Kit includes a set of standards calibrated to a known amount of exosome particles as determined by NanoSight analysis. These standards can be used to generate a calibration curve enabling quantitation of exosomes carrying CD63 from the ExoELISA data.
Supporting Data
FAQs
Related Products
Resources
Citations
-
Han, D, et al. (2024) Current Technology for Production, Isolation, and Quality Control of Extracellular Vesicles. Biomedical Applications of Extracellular Vesicles. 2024;:117-146. Link: Biomedical Applications of Extracellular Vesicles
-
Bhagwan Valjee, R, et al. (2024) Investigation of exosomal tetraspanin profile in sepsis patients as a promising diagnostic biomarker. Biomarkers : biochemical indicators of exposure, response, and susceptibility to chemicals. 2024;:1-12. PM ID: 38354024
-
Johnston, J, et al. (2024) Stimulative piezoelectric nanofibrous scaffolds for enhanced small extracellular vesicle production in 3D cultures. bioRxiv : the preprint server for biology. 2024;. PM ID: 38659930
-
Robert, M, Jakhar, R & Crasta, K. (2024) Extracellular vesicles within a tumourigenic therapy-induced senescent tumour secretome are able to confer anti-cancer properties. bioRxiv. 2024;. Link: bioRxiv
-
Dias, T, et al. (2024) An electro-optical platform for the ultrasensitive detection of small extracellular vesicle sub-types and their protein epitope counts. iScience. 2024; 27(6):109866. PM ID: 38840839
-
Hudson, B. (2024) An in Vitro Investigation of Urothelial Cell Function in Response to Hypoxia and Pressure in Relation to Bladder Outlet Obstruction. Thesis. 2024;. Link: Thesis
-
Gao, H, et al. (2024) Extracellular vesicles from organoid-derived human retinal progenitor cells prevent lipid overload-induced retinal pigment epithelium injury by regulating fatty acid metabolism. Journal of extracellular vesicles. 2024; 13(1):e12401. PM ID: 38151470
-
Byappanahalli, A, et al. (2024) Extracellular vesicle mitochondrial DNA levels are associated with race and mitochondrial DNA haplogroup. iScience. 2024; 27(1):108724. Link: iScience
-
Nagao, Y, et al. (2024) Uterine leiomyosarcoma cell-derived extracellular vesicles induce the formation of cancer-associated fibroblasts. Biochimica et Biophysica Acta (BBA) - Molecular Basis of Disease. 2024;:167103. PM ID: 38417460
-
Rajeev Kumar, S, Sakthiswary, R & Lokanathan, Y. (2024) Potential Therapeutic Application and Mechanism of Action of Stem Cell-Derived Extracellular Vesicles (EVs) in Systemic Lupus Erythematosus (SLE). International journal of molecular sciences. 2024; 25(4). PM ID: 38397121
-
Huang, Y, et al. (2024) Identification of a Serum Exosome-Derived lncRNA‒miRNA‒mRNA ceRNA Network in Patients with Endometriosis. Clinical and Experimental Obstetrics & Gynecology. 2024; 51(2):51. Link: Clinical and Experimental Obstetrics & Gynecology
-
Muraoka, A, et al. (2024) Small extracellular vesicles in follicular fluids for predicting reproductive outcomes in assisted reproductive technology. Communications medicine. 2024; 4(1):33. PM ID: 38418565
-
Liang, W, Najor, RH & Gustafsson, ÅB. (2024) Protocol to separate small and large extracellular vesicles from mouse and human cardiac tissues. STAR protocols. 2024; 5(1):102914. PM ID: 38386549
-
Otahal, A, et al. (2024) Culture of Hoffa fat pad mesenchymal stem/stromal cells on microcarrier suspension in vertical wheel bioreactor for extracellular vesicle production. Stem cell research & therapy. 2024; 15(1):61. PM ID: 38439108
-
Ibrahim, P, et al. (2024) Profiling Small RNA From Brain Extracellular Vesicles in Individuals With Depression. The international journal of neuropsychopharmacology. 2024; 27(3). PM ID: 38457375
-
Yung, C, et al. (2024) Neonatal enteroids absorb extracellular vesicles from human milk-fed infant digestive fluid. Journal of extracellular vesicles. 2024; 13(4):e12422. PM ID: 38602306
-
DeMarino, C, et al. (2024) Autophagy Deregulation in HIV-1-Infected Cells Increases Extracellular Vesicle Release and Contributes to TLR3 Activation. Viruses. 2024; 16(4). PM ID: 38675983
-
Parikh, R, et al. (2024) Recycled melanoma-secreted melanosomes regulate tumor-associated macrophage diversification. The EMBO journal. 2024;. PM ID: 38719996
-
Ye, X, et al. (2024) A novel function and mechanism of ischemia-induced retinal astrocyte-derived exosomes for RGC apoptosis of ischemic retinopathy. Molecular therapy. Nucleic acids. 2024; 35(2):102209. PM ID: 38831900
-
Hindle, J, et al. (2024) hTERT-Immortalized Mesenchymal Stem Cell-Derived Extracellular Vesicles: Large-Scale Manufacturing, Cargo Profiling, and Functional Effects in Retinal Epithelial Cells. Cells. 2024; 13(10). PM ID: 38786083
- See More
Products
Overview
Overview
Sensitive, ELISA-based exosome quantitation
When you would like to use an ELISA-based method to find out how many exosomes you have and time is not limiting, choose one of SBI’s ExoELISA Complete Kits. Calibrated using NanoSight Analysis, the ExoELISA Complete Kit (CD63 Detection) enables calculation of exosome abundance based on the presence of CD63, a general exosome marker. Note that our newer ExoELISA-ULTRA Complete Kit (CD63 Detection) can provide faster exosome quantitation using significantly less sample.
- Sensitive—detect as little as 500 µg protein equivalent
- Flexible—compatible with all major exosome isolation methods (g. ExoQuick®, ultracentrifugation, ultrafiltration, and immunoaffinity capture) from human, mouse, and rat
- Quantitative—calibrated internal standards enable quantitation of exosomes carrying CD63
References
How It Works
How It Works
Our ExoELISA Kits have all the reagents you need to run the ELISA—just add lysed exosome particles. The kits are compatible with exosomes isolated using most methods, including ExoQuick®, ExoQuick-TC®, or ultracentrifugation.
The lysed exosome particles (and, thus, exosomal proteins) are directly immobilized onto the wells of the microtiter plate, and after binding, a blocking agent is added to prevent non-specific binding of the primary detection antibody, anti-CD63. Following addition of anti-CD63, a secondary antibody (goat anti-rabbit) linked to horseradish peroxidase (HRP) is also added to amplify the signal and increase assay sensitivity.
The amount of CD63 is measured via activity of the bound HRP-secondary antibody using a colorimetric assay with extra-sensitive TMB as the substrate. The accumulation of colored product is proportional to the amount of CD63 present in each well, and is measured using a microtiter plate reader at 450 nm absorbance.
Each ExoELISA Kit includes a set of standards calibrated to a known amount of exosome particles as determined by NanoSight analysis. These standards can be used to generate a calibration curve enabling quantitation of exosomes carrying CD63 from the ExoELISA data.
Supporting Data
FAQs
Citations
-
Han, D, et al. (2024) Current Technology for Production, Isolation, and Quality Control of Extracellular Vesicles. Biomedical Applications of Extracellular Vesicles. 2024;:117-146. Link: Biomedical Applications of Extracellular Vesicles
-
Bhagwan Valjee, R, et al. (2024) Investigation of exosomal tetraspanin profile in sepsis patients as a promising diagnostic biomarker. Biomarkers : biochemical indicators of exposure, response, and susceptibility to chemicals. 2024;:1-12. PM ID: 38354024
-
Johnston, J, et al. (2024) Stimulative piezoelectric nanofibrous scaffolds for enhanced small extracellular vesicle production in 3D cultures. bioRxiv : the preprint server for biology. 2024;. PM ID: 38659930
-
Robert, M, Jakhar, R & Crasta, K. (2024) Extracellular vesicles within a tumourigenic therapy-induced senescent tumour secretome are able to confer anti-cancer properties. bioRxiv. 2024;. Link: bioRxiv
-
Dias, T, et al. (2024) An electro-optical platform for the ultrasensitive detection of small extracellular vesicle sub-types and their protein epitope counts. iScience. 2024; 27(6):109866. PM ID: 38840839
-
Hudson, B. (2024) An in Vitro Investigation of Urothelial Cell Function in Response to Hypoxia and Pressure in Relation to Bladder Outlet Obstruction. Thesis. 2024;. Link: Thesis
-
Gao, H, et al. (2024) Extracellular vesicles from organoid-derived human retinal progenitor cells prevent lipid overload-induced retinal pigment epithelium injury by regulating fatty acid metabolism. Journal of extracellular vesicles. 2024; 13(1):e12401. PM ID: 38151470
-
Byappanahalli, A, et al. (2024) Extracellular vesicle mitochondrial DNA levels are associated with race and mitochondrial DNA haplogroup. iScience. 2024; 27(1):108724. Link: iScience
-
Nagao, Y, et al. (2024) Uterine leiomyosarcoma cell-derived extracellular vesicles induce the formation of cancer-associated fibroblasts. Biochimica et Biophysica Acta (BBA) - Molecular Basis of Disease. 2024;:167103. PM ID: 38417460
-
Rajeev Kumar, S, Sakthiswary, R & Lokanathan, Y. (2024) Potential Therapeutic Application and Mechanism of Action of Stem Cell-Derived Extracellular Vesicles (EVs) in Systemic Lupus Erythematosus (SLE). International journal of molecular sciences. 2024; 25(4). PM ID: 38397121
-
Huang, Y, et al. (2024) Identification of a Serum Exosome-Derived lncRNA‒miRNA‒mRNA ceRNA Network in Patients with Endometriosis. Clinical and Experimental Obstetrics & Gynecology. 2024; 51(2):51. Link: Clinical and Experimental Obstetrics & Gynecology
-
Muraoka, A, et al. (2024) Small extracellular vesicles in follicular fluids for predicting reproductive outcomes in assisted reproductive technology. Communications medicine. 2024; 4(1):33. PM ID: 38418565
-
Liang, W, Najor, RH & Gustafsson, ÅB. (2024) Protocol to separate small and large extracellular vesicles from mouse and human cardiac tissues. STAR protocols. 2024; 5(1):102914. PM ID: 38386549
-
Otahal, A, et al. (2024) Culture of Hoffa fat pad mesenchymal stem/stromal cells on microcarrier suspension in vertical wheel bioreactor for extracellular vesicle production. Stem cell research & therapy. 2024; 15(1):61. PM ID: 38439108
-
Ibrahim, P, et al. (2024) Profiling Small RNA From Brain Extracellular Vesicles in Individuals With Depression. The international journal of neuropsychopharmacology. 2024; 27(3). PM ID: 38457375
-
Yung, C, et al. (2024) Neonatal enteroids absorb extracellular vesicles from human milk-fed infant digestive fluid. Journal of extracellular vesicles. 2024; 13(4):e12422. PM ID: 38602306
-
DeMarino, C, et al. (2024) Autophagy Deregulation in HIV-1-Infected Cells Increases Extracellular Vesicle Release and Contributes to TLR3 Activation. Viruses. 2024; 16(4). PM ID: 38675983
-
Parikh, R, et al. (2024) Recycled melanoma-secreted melanosomes regulate tumor-associated macrophage diversification. The EMBO journal. 2024;. PM ID: 38719996
-
Ye, X, et al. (2024) A novel function and mechanism of ischemia-induced retinal astrocyte-derived exosomes for RGC apoptosis of ischemic retinopathy. Molecular therapy. Nucleic acids. 2024; 35(2):102209. PM ID: 38831900
-
Hindle, J, et al. (2024) hTERT-Immortalized Mesenchymal Stem Cell-Derived Extracellular Vesicles: Large-Scale Manufacturing, Cargo Profiling, and Functional Effects in Retinal Epithelial Cells. Cells. 2024; 13(10). PM ID: 38786083
- See More