ExoELISA Complete Kit (CD63 Detection)

A sensitive ELISA assay for exosome quantitation based on the presence of CD63, a general exosome marker. Compatible with most biofluids.

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Exosome ELISA Complete Kit (CD63 detection)

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EXOEL-CD63A-1
$ 670
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Overview

Sensitive, ELISA-based exosome quantitation

When you would like to use an ELISA-based method to find out how many exosomes you have and time is not limiting, choose one of SBI’s ExoELISA Complete Kits. Calibrated using NanoSight Analysis, the ExoELISA Complete Kit (CD63 Detection) enables calculation of exosome abundance based on the presence of CD63, a general exosome marker. Note that our newer ExoELISA-ULTRA Complete Kit (CD63 Detection) can provide faster exosome quantitation using significantly less sample.

  • Sensitive—detect as little as 500 µg protein equivalent
  • Flexible—compatible with all major exosome isolation methods (g. ExoQuick®, ultracentrifugation, ultrafiltration, and immunoaffinity capture) from human, mouse, and rat
  • Quantitative—calibrated internal standards enable quantitation of exosomes carrying CD63

Choose the exosome quantitation method that’s best for your studies

  ExoELISA-ULTRA CD63
ExoELISA-ULTRA CD81
ExoELISA CD9
ExoELISA CD63
ExoELISA CD81
EXOCET FluoroCet
Use For fast and sensitive antibody-based quantitation of exosomes For sensitive quantitation of exosomes when time and input sample are not limiting For fast quantitation of extracellular vesicles with moderate sample input requirements For the most sensitive quantitation of extracellular vesicles with very low sample input requirements
Detection method Antibody Antibody Enzymatic Enzymatic
Quantitation chemistry Enzymatic (HRP) Enzymatic (HRP) Colorimetric Fluorescent
Total protocol time 4 hours (no overnight incubation) 24 hours 20 min 60 min
Input sample amount (protein equivalent) 1 – 200 µg >500 µg 50 µg <1 µg
Learn More ExoELISA-ULTRA CD63

ExoELISA-ULTRA CD81

ExoELISA CD9
ExoELISA CD63
ExoELISA CD81
EXOCET FluoroCet

How It Works

Our ExoELISA Kits have all the reagents you need to run the ELISA—just add lysed exosome particles. The kits are compatible with exosomes isolated using most methods, including ExoQuick®, ExoQuick-TC®, or ultracentrifugation.

The ExoELISA assay uses a colorimetric, HRP activity-based readout using extra-sensitive TMB as the substrate.

The lysed exosome particles (and, thus, exosomal proteins) are directly immobilized onto the wells of the microtiter plate, and after binding, a blocking agent is added to prevent non-specific binding of the primary detection antibody, anti-CD63. Following addition of anti-CD63, a secondary antibody (goat anti-rabbit) linked to horseradish peroxidase (HRP) is also added to amplify the signal and increase assay sensitivity.

The amount of CD63 is measured via activity of the bound HRP-secondary antibody using a colorimetric assay with extra-sensitive TMB as the substrate. The accumulation of colored product is proportional to the amount of CD63 present in each well, and is measured using a microtiter plate reader at 450 nm absorbance.

Each ExoELISA Kit includes a set of standards calibrated to a known amount of exosome particles as determined by NanoSight analysis. These standards can be used to generate a calibration curve enabling quantitation of exosomes carrying CD63 from the ExoELISA data.An example ExoELISA CD63 calibration curve using the included exosome standards.The number of exosome particles in the standards supplied with each ExoELISA Kit are quantified via NanoSight Analysis.


Citations

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