Simplify SparQ projects with an all-in-one vector
With SBI’s SparQ™ Cumate Switch System, you can get inducible gene expression in mammalian cells with a range of cloning and expression lentivectors. The pCDH-CuO-MCS-IRES-GFP-EF1α-CymR-T2A-Puro SparQ All-in-one Cloning and Expression Lentivector (Cat.# QM812B-1) simplifies your SparQ projects by co-expressing both your gene- or miRNA-of-interest and a GFP marker from the inducible cumate switch promoter, enabling measurement of induction by GFP fluorescence*. Co-expression is mediated by an IRES element. The vector also constitutively co-expresses CymR and a puromycin resistance marker from an EF1α promoter, with co-expression mediated by the T2A element.
With SBI’s SparQ™ Cumate Switch System, you can get inducible gene expression in mammalian cells through the binding of cumate, a non-toxic small molecule, to CymR. Expression levels of your gene-of-interest are tightly controlled and increase with increasing cumate concentration until maximum induction is reached—see as much as a 32-fold increase in gene expression. Even better, induction is reversible, so you can turn expression levels on and off. Delivering negligible background expression in the absence of cumate, the SparQ System is an excellent choice for achieving controlled levels of gene expression.
- Robust—increase expression up to 32-fold
- Adjustable—tune expression levels by titrating the amount of cumate
- Reversible—turn expression on, then off, then on again
- Versatile—choose from all-in-one formats that co-express CymR and your gene-of-interest, or two-vector systems where CymR is expressed from a different plasmid
- Powerful—suitable for in vivo applications
*NOTE: Due to the size of the QM812B-1 lentivector, you may only insert up to 2 kb of DNA into the MCS. A >1 kb insert will deliver excellent expression as shown below with the 1.3 kb mouse Foxp3 cDNA cloned into the QM812B-1 vector and induced with 300 µg/mL cumate.